Spdef

Functionⁱ

May function as an androgen-independent transactivator of the prostate-specific antigen (PSA) promoter. Binds to 5'-GGAT-3' DNA sequences. May play a role in the regulation of the prostate gland and/or prostate cancer development. Acts as a transcriptional activator for SERPINB5 promoter (By similarity).By similarity1 Publication

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
DNA bindingⁱ	239 – 322	ETSPROSITE-ProRule annotationAdd BLAST		84

GO - Molecular functionⁱ

DNA-binding transcription factor activity, RNA polymerase II-specific
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
sequence-specific DNA binding Source: MGI

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

cell differentiation
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
intestinal epithelial cell development
Source: MGI
Inferred from mutant phenotypeⁱ
- 19549527
lung goblet cell differentiation
Source: MGI
Inferred from direct assayⁱ
- 17347682
negative regulation of cell fate commitment
Source: MGI
Inferred from mutant phenotypeⁱ
- 19759516
negative regulation of transcription by RNA polymerase II
Source: MGI
Inferred from direct assayⁱ
- 19759516
positive regulation of apoptotic process Source: MGI
positive regulation of cell fate commitment
Source: MGI
Inferred from mutant phenotypeⁱ
- 19759516
positive regulation of transcription by RNA polymerase II
Source: MGI
Inferred from direct assayⁱ
- 17347682
- 19759516
regulation of transcription by RNA polymerase II
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Activator, DNA-binding
Biological process	Transcription, Transcription regulation

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: SAM pointed domain-containing Ets transcription factor Alternative name(s): Prostate epithelium-specific Ets transcription factor Short name: Prostate-specific Ets Prostate-derived Ets factor
Gene namesⁱ	Name:Spdef Synonyms:Pdef, Pse
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 17

Organism-specific databases

MGIⁱ	MGI:1353422 Spdef

Keywords - Cellular componentⁱ

Nucleus

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000223959	1 – 325	SAM pointed domain-containing Ets transcription factorAdd BLAST		325

Proteomic databases

PaxDbⁱ	Q9WTP3
PRIDEⁱ	Q9WTP3

PTM databases

PhosphoSitePlusⁱ	Q9WTP3

PhosphoSitePlusⁱ

Q9WTP3

Expressionⁱ

Tissue specificityⁱ

Expressed in the accessory glands of sex organs including the prostate, seminal vesicle, coagulating gland in males, the oviduct in females, and in intestines. Expression is epithelial-specific.1 Publication

Gene expression databases

Bgeeⁱ	ENSMUSG00000024215 Expressed in 99 organ(s), highest expression level in head
ExpressionAtlasⁱ	Q9WTP3 baseline and differential
Genevisibleⁱ	Q9WTP3 MM

Interactionⁱ

Subunit structureⁱ

Interacts with the DNA-binding domain of the androgen receptor. Interacts with NKX3-1 (By similarity).By similarity

Protein-protein interaction databases

BioGridⁱ	205953, 2 interactors
STRINGⁱ	10090.ENSMUSP00000025054

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q9WTP3
SMRⁱ	Q9WTP3
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	119 – 203	PNTPROSITE-ProRule annotationAdd BLAST		85

Sequence similaritiesⁱ

Belongs to the ETS family.Curated

Phylogenomic databases

eggNOGⁱ	KOG3805 Eukaryota ENOG410XSXU LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00760000118996
HOGENOMⁱ	HOG000237327
HOVERGENⁱ	HBG080776
InParanoidⁱ	Q9WTP3
KEGG Orthology (KO) More...KOⁱ	K09442
OMAⁱ	DEPEQCP
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G07NK
PhylomeDBⁱ	Q9WTP3
TreeFamⁱ	TF318679

Family and domain databases

Gene3Dⁱ	1.10.10.10, 1 hit
InterProⁱ	View protein in InterPro IPR000418 Ets_dom IPR003118 Pointed_dom IPR013761 SAM/pointed_sf IPR036388 WH-like_DNA-bd_sf IPR036390 WH_DNA-bd_sf
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00178 Ets, 1 hit PF02198 SAM_PNT, 1 hit
PRINTSⁱ	PR00454 ETSDOMAIN
SMARTⁱ	View protein in SMART SM00413 ETS, 1 hit SM00251 SAM_PNT, 1 hit
SUPFAMⁱ	SSF46785 SSF46785, 1 hit SSF47769 SSF47769, 1 hit
PROSITEⁱ	View protein in PROSITE PS00345 ETS_DOMAIN_1, 1 hit PS00346 ETS_DOMAIN_2, 1 hit PS50061 ETS_DOMAIN_3, 1 hit PS51433 PNT, 1 hit

Sequence (1+)ⁱ

Sequence statusⁱ: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show all Align All

Q9WTP3-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MGSASPGLSN VSPGCLLLFP DVAPRTGTEK AASGAMGPEK QEWSPSPPAT 
        60         70         80         90        100
PEQGLSAFYL SYFNMYPDDS SWVAKVPEAR AGEDHPEEPE QCPVIDSQAS 
       110        120        130        140        150
GSTLDEHSLE QVQSMVVGEV LKDIETACKL LNITADPGDW SPGNVQKWLL 
       160        170        180        190        200
WTEHQYRLPP AGKAFQELGG KELCAMSEEQ FRQRAPLGGD VLHAHLDIWK 
       210        220        230        240        250
SAAWMKERTS PGTLHYCAST SEEGWTDGEV DSSCSGQPIH LWQFLKELLL 
       260        270        280        290        300
KPHSYGRFIR WLNKEKGIFK IEDSAQVARL WGVRKNRPAM NYDKLSRSIR 
       310        320 
QYYKKGIIRK PDISQRLVYQ FVHPV

Length:325

Mass (Da):36,356

Last modified:November 1, 1999 - v1

Checksum:ⁱB55CF6CEC4501125

GO

Computationally mapped potential isoform sequencesⁱ

There is 1 potential isoform mapped to this entry.BLAST Align Show all Add to basket

	Entry	Entry name		Protein names	Gene names	Length	Annotation

	B2KF87	B2KF87_MOUSE		SAM pointed domain-containing Ets t... SAM pointed domain-containing Ets transcription factor	Spdef	126	Annotation score:

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AB019436 mRNA Translation: BAA77329.1 AK078863 mRNA Translation: BAC37426.1 BC012648 mRNA Translation: AAH12648.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS28568.1
NCBI Reference Sequences More...RefSeqⁱ	NP_038919.1, NM_013891.4 XP_006524442.1, XM_006524379.3 XP_006524443.1, XM_006524380.3 XP_006524444.1, XM_006524381.3 XP_017172988.1, XM_017317499.1
UniGeneⁱ	Mm.26768

Genome annotation databases

Ensemblⁱ	ENSMUST00000025054; ENSMUSP00000025054; ENSMUSG00000024215 ENSMUST00000114870; ENSMUSP00000110520; ENSMUSG00000024215 ENSMUST00000167489; ENSMUSP00000127056; ENSMUSG00000024215
GeneIDⁱ	30051
KEGGⁱ	mmu:30051
UCSC genome browser More...UCSCⁱ	uc008bpo.2 mouse

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q9WTP3	SAM pointed domain-containing Ets transcription factor (Fragment)		MOUSE		126	UniRef100_Q9WTP3

Protein	Similar proteins		Species	Score	Length	Source
Q9WTP3	SAM pointed domain-containing Ets transcription factor (Fragment)		MOUSE		126	UniRef90_Q9WTP3
RCG60727		RAT		325
SAM pointed domain-containing Ets transcription factor isoform X1		MESAU		325
SAM pointed domain-containing Ets transcription factor		CRIGR		325
Uncharacterized protein (Fragment)		NEOLE		311
+13

Protein	Similar proteins		Species	Score	Length	Source
Q9WTP3	Isoform 2 of SAM pointed domain-containing Ets transcription factor		HUMAN		319	UniRef50_O95238-2
Uncharacterized protein		PIG		363
Uncharacterized protein		PROCO		319
Uncharacterized protein		CALJA		319
Uncharacterized protein		CERAT		319
+280

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AB019436 mRNA Translation: BAA77329.1 AK078863 mRNA Translation: BAC37426.1 BC012648 mRNA Translation: AAH12648.1
CCDSⁱ	CCDS28568.1
RefSeqⁱ	NP_038919.1, NM_013891.4 XP_006524442.1, XM_006524379.3 XP_006524443.1, XM_006524380.3 XP_006524444.1, XM_006524381.3 XP_017172988.1, XM_017317499.1
UniGeneⁱ	Mm.26768

3D structure databases

ProteinModelPortalⁱ	Q9WTP3
SMRⁱ	Q9WTP3
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	205953, 2 interactors
STRINGⁱ	10090.ENSMUSP00000025054

PTM databases

PhosphoSitePlusⁱ	Q9WTP3

PhosphoSitePlusⁱ

Q9WTP3

Proteomic databases

PaxDbⁱ	Q9WTP3
PRIDEⁱ	Q9WTP3

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000025054; ENSMUSP00000025054; ENSMUSG00000024215 ENSMUST00000114870; ENSMUSP00000110520; ENSMUSG00000024215 ENSMUST00000167489; ENSMUSP00000127056; ENSMUSG00000024215
GeneIDⁱ	30051
KEGGⁱ	mmu:30051
UCSCⁱ	uc008bpo.2 mouse

Organism-specific databases

CTDⁱ	25803
MGIⁱ	MGI:1353422 Spdef

Phylogenomic databases

eggNOGⁱ	KOG3805 Eukaryota ENOG410XSXU LUCA
GeneTreeⁱ	ENSGT00760000118996
HOGENOMⁱ	HOG000237327
HOVERGENⁱ	HBG080776
InParanoidⁱ	Q9WTP3
KOⁱ	K09442
OMAⁱ	DEPEQCP
OrthoDBⁱ	EOG091G07NK
PhylomeDBⁱ	Q9WTP3
TreeFamⁱ	TF318679

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:Q9WTP3
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000024215 Expressed in 99 organ(s), highest expression level in head
ExpressionAtlasⁱ	Q9WTP3 baseline and differential
Genevisibleⁱ	Q9WTP3 MM

Family and domain databases

Gene3Dⁱ	1.10.10.10, 1 hit
InterProⁱ	View protein in InterPro IPR000418 Ets_dom IPR003118 Pointed_dom IPR013761 SAM/pointed_sf IPR036388 WH-like_DNA-bd_sf IPR036390 WH_DNA-bd_sf
Pfamⁱ	View protein in Pfam PF00178 Ets, 1 hit PF02198 SAM_PNT, 1 hit
PRINTSⁱ	PR00454 ETSDOMAIN
SMARTⁱ	View protein in SMART SM00413 ETS, 1 hit SM00251 SAM_PNT, 1 hit
SUPFAMⁱ	SSF46785 SSF46785, 1 hit SSF47769 SSF47769, 1 hit
PROSITEⁱ	View protein in PROSITE PS00345 ETS_DOMAIN_1, 1 hit PS00346 ETS_DOMAIN_2, 1 hit PS50061 ETS_DOMAIN_3, 1 hit PS51433 PNT, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	SPDEF_MOUSE
Accessionⁱ	Q9WTP3Primary (citable) accession number: Q9WTP3
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	February 21, 2006
	Last sequence update:	November 1, 1999
	Last modified:	November 7, 2018
	This is version 145 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q9WTP3 (SPDEF_MOUSE)

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SAM pointed domain-containing Ets transcription factor

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Nucleus

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ