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Protein

Protein sprouty homolog 4

Gene

Spry4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Suppresses the insulin receptor and EGFR-transduced MAPK signaling pathway, but does not inhibit MAPK activation by a constitutively active mutant Ras. Probably impairs the formation of GTP-Ras (By similarity). Inhibits Ras-independent, but not Ras-dependent, activation of RAF1 (By similarity).By similarity

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein

Names & Taxonomyi

Protein namesi
Recommended name:
Protein sprouty homolog 4
Short name:
Spry-4
Gene namesi
Name:Spry4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:1345144 Spry4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000769061 – 300Protein sprouty homolog 4Add BLAST300

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei126PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ9WTP2
PaxDbiQ9WTP2
PeptideAtlasiQ9WTP2
PRIDEiQ9WTP2

PTM databases

iPTMnetiQ9WTP2
PhosphoSitePlusiQ9WTP2
SwissPalmiQ9WTP2

Expressioni

Tissue specificityi

Expressed in the embryo and adult tissues including heart, brain, lung, kidney, and skeletal muscle.

Developmental stagei

At 8 dpc expressed in the lateral plate mesoderm of the primitive streak. At 9.5 and 10.5 dpc expressed in the nasal placodes, maxillary and mandibular processes, posterior part of the hyoid arch and the progress zone of the limb buds and the presomitic mesoderm. At 11.5 dpc expressed in the dorso-lateral region of the somites (mostly in the myotome) and in the otic vesicle. At 11.5 and 12.5 dpc expressed in the distal lung mesenchyme, with a strong expression in the accessory lobe of the lung.1 Publication

Inductioni

By FGF signaling.

Gene expression databases

BgeeiENSMUSG00000024427 Expressed in 272 organ(s), highest expression level in primary oocyte
CleanExiMM_SPRY4
GenevisibleiQ9WTP2 MM

Interactioni

Subunit structurei

Interacts with the C-terminus of TESK1. Interacts with RAF1.By similarity

Protein-protein interaction databases

BioGridi204878, 7 interactors
STRINGi10090.ENSMUSP00000025295

Structurei

3D structure databases

ProteinModelPortaliQ9WTP2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini167 – 274SPRPROSITE-ProRule annotationAdd BLAST108

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi98 – 108Poly-SerAdd BLAST11
Compositional biasi160 – 284Cys-richAdd BLAST125

Domaini

The Cys-rich domain is responsible for the localization of the protein to the membrane ruffles.

Sequence similaritiesi

Belongs to the sprouty family.Curated

Phylogenomic databases

eggNOGiENOG410IH70 Eukaryota
ENOG410ZPNT LUCA
GeneTreeiENSGT00390000003535
HOGENOMiHOG000233079
HOVERGENiHBG003544
InParanoidiQ9WTP2
KOiK17385
OMAiPPELDKH
OrthoDBiEOG091G0DOQ
PhylomeDBiQ9WTP2
TreeFamiTF325070

Family and domain databases

InterProiView protein in InterPro
IPR007875 Sprouty
IPR030790 SPRY4
PANTHERiPTHR12365:SF6 PTHR12365:SF6, 1 hit
PfamiView protein in Pfam
PF05210 Sprouty, 1 hit
PROSITEiView protein in PROSITE
PS51227 SPR, 1 hit

Sequencei

Sequence statusi: Complete.

Q9WTP2-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEPPVPQSSV PVNPSSVMVQ PLLDSRAPHS RLQHPLTILP IDQMKTSHVE
60 70 80 90 100
NDYIDNPSLA PATGPKRPRG GPPELAPTPA RCDQDITHHW ISFSGRPSSV
110 120 130 140 150
SSSSSTSSDQ RLLDHMAPPP VAEQASPRAV RLQPKVVHCK PLDLKGPTAP
160 170 180 190 200
PELDKHFLLC EACGKCKCKE CASPRTLPSC WVCNQECLCS AQTLVNYGTC
210 220 230 240 250
MCLVQGIFYH CTNEDDEGSC ADHPCSCSGS NCCARWSFMG ALSVVLPCLL
260 270 280 290 300
CYLPATGCVK LAQRGYDRLR RPGCRCKHTN SVICKAASGD TKTSRSDKPF
Length:300
Mass (Da):32,523
Last modified:November 1, 1999 - v1
Checksum:iDA963036EFC0E73F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti10V → F in AAD56007 (PubMed:10498682).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB019280 mRNA Translation: BAA77689.1
AF176906 mRNA Translation: AAD56007.1
AK037180 mRNA Translation: BAC29739.1
AK048122 mRNA Translation: BAC33249.1
CH466528 Genomic DNA Translation: EDL10072.1
BC050944 mRNA Translation: AAH50944.1
BC057005 mRNA Translation: AAH57005.1
CCDSiCCDS29202.1
RefSeqiNP_036028.2, NM_011898.2
UniGeneiMm.42038

Genome annotation databases

EnsembliENSMUST00000025295; ENSMUSP00000025295; ENSMUSG00000024427
GeneIDi24066
KEGGimmu:24066
UCSCiuc008esl.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB019280 mRNA Translation: BAA77689.1
AF176906 mRNA Translation: AAD56007.1
AK037180 mRNA Translation: BAC29739.1
AK048122 mRNA Translation: BAC33249.1
CH466528 Genomic DNA Translation: EDL10072.1
BC050944 mRNA Translation: AAH50944.1
BC057005 mRNA Translation: AAH57005.1
CCDSiCCDS29202.1
RefSeqiNP_036028.2, NM_011898.2
UniGeneiMm.42038

3D structure databases

ProteinModelPortaliQ9WTP2
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204878, 7 interactors
STRINGi10090.ENSMUSP00000025295

PTM databases

iPTMnetiQ9WTP2
PhosphoSitePlusiQ9WTP2
SwissPalmiQ9WTP2

Proteomic databases

MaxQBiQ9WTP2
PaxDbiQ9WTP2
PeptideAtlasiQ9WTP2
PRIDEiQ9WTP2

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000025295; ENSMUSP00000025295; ENSMUSG00000024427
GeneIDi24066
KEGGimmu:24066
UCSCiuc008esl.1 mouse

Organism-specific databases

CTDi81848
MGIiMGI:1345144 Spry4

Phylogenomic databases

eggNOGiENOG410IH70 Eukaryota
ENOG410ZPNT LUCA
GeneTreeiENSGT00390000003535
HOGENOMiHOG000233079
HOVERGENiHBG003544
InParanoidiQ9WTP2
KOiK17385
OMAiPPELDKH
OrthoDBiEOG091G0DOQ
PhylomeDBiQ9WTP2
TreeFamiTF325070

Miscellaneous databases

PROiPR:Q9WTP2
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000024427 Expressed in 272 organ(s), highest expression level in primary oocyte
CleanExiMM_SPRY4
GenevisibleiQ9WTP2 MM

Family and domain databases

InterProiView protein in InterPro
IPR007875 Sprouty
IPR030790 SPRY4
PANTHERiPTHR12365:SF6 PTHR12365:SF6, 1 hit
PfamiView protein in Pfam
PF05210 Sprouty, 1 hit
PROSITEiView protein in PROSITE
PS51227 SPR, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSPY4_MOUSE
AccessioniPrimary (citable) accession number: Q9WTP2
Secondary accession number(s): Q543R1, Q9QXV7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: November 1, 1999
Last modified: November 7, 2018
This is version 118 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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