UniProtKB - Q9WTK8 (SPO11_MOUSE)
Protein
Meiotic recombination protein SPO11
Gene
Spo11
Organism
Mus musculus (Mouse)
Status
Functioni
Component of a topoisomerase 6 complex specifically required for meiotic recombination. Together with TOP6BL, mediates DNA cleavage that forms the double-strand breaks (DSB) that initiate meiotic recombination (PubMed:26917764). The complex promotes relaxation of negative and positive supercoiled DNA and DNA decatenation through cleavage and ligation cycles. Essential for the phosphorylation of SMC3, HORMAD1 and HORMAD2 (PubMed:22346761).2 Publications
In contrast to isoform 1, does not mediate DNA cleavage that forms the double-strand breaks (DSB) that initiate meiotic recombination.1 Publication
Catalytic activityi
- ATP-dependent breakage, passage and rejoining of double-stranded DNA.Curated EC:5.6.2.2
Cofactori
Mg2+By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 138 | NucleophileBy similarity | 1 | |
Metal bindingi | 224 | MagnesiumBy similarity | 1 | |
Metal bindingi | 277 | MagnesiumBy similarity | 1 |
GO - Molecular functioni
- ATP binding Source: InterPro
- DNA binding Source: GO_Central
- DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity Source: UniProtKB-EC
- endodeoxyribonuclease activity, producing 3'-phosphomonoesters Source: GO_Central
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- double-strand break repair involved in meiotic recombination Source: MGI
- homologous chromosome pairing at meiosis Source: MGI
- male meiosis I Source: MGI
- meiotic DNA double-strand break formation Source: GO_Central
- meiotic DNA double-strand break processing Source: GO_Central
- meiotic telomere clustering Source: MGI
- oogenesis Source: MGI
- ovarian follicle development Source: MGI
- protein localization to chromosome Source: MGI
- reciprocal meiotic recombination Source: MGI
- spermatid development Source: MGI
- synaptonemal complex assembly Source: MGI
Keywordsi
Molecular function | DNA-binding, Hydrolase, Isomerase |
Biological process | Meiosis |
Ligand | Magnesium, Metal-binding |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Spo11 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1349669, Spo11 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Nucleus
- nuclear chromosome Source: GO_Central
- nucleoplasm Source: Reactome
Other locations
- chromosome, telomeric region Source: MGI
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000145475 | 1 – 396 | Meiotic recombination protein SPO11Add BLAST | 396 |
Proteomic databases
PaxDbi | Q9WTK8 |
PRIDEi | Q9WTK8 |
PTM databases
iPTMneti | Q9WTK8 |
PhosphoSitePlusi | Q9WTK8 |
Expressioni
Tissue specificityi
High levels are found only in the testis where expression is restricted primarily to meiotic germ cells. Not expressed in spermatogonia. Highest levels are found in pachytene spermatocytes. Very low levels are found in thymus, brain and oocytes of embryonic ovary. Not detected in adult ovary (PubMed:10622720, PubMed:10855504). Isoform 1: Expressed early in meiosis, when most double-strand breaks (DSB) are formed (PubMed:21330546).4 Publications
Developmental stagei
Not detected at day 7 postpartum (dpp). Levels are low at 12 dpp but increase by 17 dpp. High levels are maintained throughout the remainder of testis development.2 Publications
Gene expression databases
Bgeei | ENSMUSG00000005883, Expressed in spermatocyte and 60 other tissues |
Genevisiblei | Q9WTK8, MM |
Interactioni
Subunit structurei
Heterotetramer of SPO11 and 2 TOP6BL chains (Probable).
Interacts with TOP6BL (PubMed:26917764).
Curated1 PublicationBinary interactionsi
Isoform 1 [Q9WTK8-1]
With | #Exp. | IntAct |
---|---|---|
Top6bl [J3QMY9] | 6 | EBI-16201014,EBI-16200997 |
Protein-protein interaction databases
BioGRIDi | 205092, 1 interactor |
DIPi | DIP-62006N |
IntActi | Q9WTK8, 1 interactor |
STRINGi | 10090.ENSMUSP00000059056 |
Miscellaneous databases
RNActi | Q9WTK8, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the TOP6A family.Curated
Phylogenomic databases
eggNOGi | KOG2795, Eukaryota |
GeneTreei | ENSGT00390000001787 |
HOGENOMi | CLU_037229_1_1_1 |
InParanoidi | Q9WTK8 |
OMAi | FTDGDPY |
OrthoDBi | 1272299at2759 |
PhylomeDBi | Q9WTK8 |
TreeFami | TF314157 |
Family and domain databases
CDDi | cd00223, TOPRIM_TopoIIB_SPO, 1 hit |
Gene3Di | 1.10.10.10, 1 hit |
InterProi | View protein in InterPro IPR004084, Meiosis_Spo11 IPR013048, Meiotic_Spo11 IPR002815, Spo11/TopoVI_A IPR013049, Spo11/TopoVI_A_N IPR036078, Spo11/TopoVI_A_sf IPR034136, TOPRIM_Topo6A/Spo11 IPR036388, WH-like_DNA-bd_sf |
PANTHERi | PTHR10848, PTHR10848, 1 hit |
Pfami | View protein in Pfam PF03533, SPO11_like, 1 hit PF04406, TP6A_N, 1 hit |
PRINTSi | PR01551, SPO11HOMOLOG PR01550, TOP6AFAMILY |
SUPFAMi | SSF56726, SSF56726, 1 hit |
s (5)i Sequence
Sequence statusi: Complete.
This entry describes 5 produced by isoformsialternative splicing. AlignAdd to basketIsoform 1 (identifier: Q9WTK8-1) [UniParc]FASTAAdd to basket
Also known as: Spo11beta1 Publication, Beta1 Publication
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAFAPMGPEA SFFDALDRHR ASLLAMVKRG AGETPAGATR VASSSEVLTA
60 70 80 90 100
IENIIQDIIK SLARNEVPAF TIDNRSSWEN IMFDDSVGLR MIPQCTTRKI
110 120 130 140 150
RSDSPKSVKK FALILKVLSM IYKLIQSDTY ATKRDIYYTD SQLFGNQAAV
160 170 180 190 200
DSAIDDISCM LKVPRRSLHV LSTSKGLIAG NLRYMEEDGT RVQCTCSATA
210 220 230 240 250
TAVPTNIQGM QHLITDAKFL LIVEKDATFQ RLLDDNFCSR MSPCIMVTGK
260 270 280 290 300
GVPDLNTRLL VKKLWDTFHI PVFTLVDADP YGIEIMCIYK YGSMSMSFEA
310 320 330 340 350
HNLTIPTIRW LGLLPSDIQR LNIPKDSLIP LTKHDQMKLD SILKRPYITY
360 370 380 390
QPLWKKELEM MADSKMKAEI QALTLLSSDY LSRVYLPNKL RFGGWI
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_007197 | 44 – 81 | Missing in isoform 3, isoform 4 and isoform 5. 3 PublicationsAdd BLAST | 38 | |
Alternative sequenceiVSP_007198 | 82 | M → R in isoform 3, isoform 4 and isoform 5. 3 Publications | 1 | |
Alternative sequenceiVSP_007199 | 134 | R → RSNAHSVLTLHLHR in isoform 3. 2 Publications | 1 | |
Alternative sequenceiVSP_007200 | 213 – 216 | Missing in isoform 2 and isoform 5. 2 Publications | 4 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF126400 mRNA Translation: AAD49561.1 AF149309 mRNA Translation: AAD44811.1 AF169386 mRNA Translation: AAD52563.1 AF163053 mRNA Translation: AAD43588.1 AF163052, AF163049, AF163051 Genomic DNA Translation: AAD43591.1 AF163052, AF163049, AF163051 Genomic DNA Translation: AAD43592.1 AF163054 mRNA Translation: AAD43589.1 AF165313 mRNA Translation: AAF87090.1 AF167439 mRNA Translation: AAF87094.1 AF173848 mRNA Translation: AAF87096.1 AF443910 Genomic DNA Translation: AAL32063.1 |
CCDSi | CCDS17137.1 [Q9WTK8-1] CCDS50811.1 [Q9WTK8-3] CCDS50812.1 [Q9WTK8-4] |
RefSeqi | NP_001077428.1, NM_001083959.1 [Q9WTK8-3] NP_001077429.1, NM_001083960.1 [Q9WTK8-4] NP_001292363.1, NM_001305434.1 [Q9WTK8-5] NP_036176.1, NM_012046.2 [Q9WTK8-1] |
Genome annotation databases
Ensembli | ENSMUST00000050442; ENSMUSP00000059056; ENSMUSG00000005883 [Q9WTK8-1] ENSMUST00000109125; ENSMUSP00000104753; ENSMUSG00000005883 [Q9WTK8-4] ENSMUST00000109126; ENSMUSP00000104754; ENSMUSG00000005883 [Q9WTK8-3] |
GeneIDi | 26972 |
KEGGi | mmu:26972 |
UCSCi | uc008odd.1, mouse [Q9WTK8-1] uc008ode.1, mouse [Q9WTK8-4] uc008odf.1, mouse [Q9WTK8-3] uc012ckq.1, mouse [Q9WTK8-5] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF126400 mRNA Translation: AAD49561.1 AF149309 mRNA Translation: AAD44811.1 AF169386 mRNA Translation: AAD52563.1 AF163053 mRNA Translation: AAD43588.1 AF163052, AF163049, AF163051 Genomic DNA Translation: AAD43591.1 AF163052, AF163049, AF163051 Genomic DNA Translation: AAD43592.1 AF163054 mRNA Translation: AAD43589.1 AF165313 mRNA Translation: AAF87090.1 AF167439 mRNA Translation: AAF87094.1 AF173848 mRNA Translation: AAF87096.1 AF443910 Genomic DNA Translation: AAL32063.1 |
CCDSi | CCDS17137.1 [Q9WTK8-1] CCDS50811.1 [Q9WTK8-3] CCDS50812.1 [Q9WTK8-4] |
RefSeqi | NP_001077428.1, NM_001083959.1 [Q9WTK8-3] NP_001077429.1, NM_001083960.1 [Q9WTK8-4] NP_001292363.1, NM_001305434.1 [Q9WTK8-5] NP_036176.1, NM_012046.2 [Q9WTK8-1] |
3D structure databases
SMRi | Q9WTK8 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 205092, 1 interactor |
DIPi | DIP-62006N |
IntActi | Q9WTK8, 1 interactor |
STRINGi | 10090.ENSMUSP00000059056 |
PTM databases
iPTMneti | Q9WTK8 |
PhosphoSitePlusi | Q9WTK8 |
Proteomic databases
PaxDbi | Q9WTK8 |
PRIDEi | Q9WTK8 |
Protocols and materials databases
Antibodypediai | 28951, 189 antibodies |
DNASUi | 26972 |
Genome annotation databases
Ensembli | ENSMUST00000050442; ENSMUSP00000059056; ENSMUSG00000005883 [Q9WTK8-1] ENSMUST00000109125; ENSMUSP00000104753; ENSMUSG00000005883 [Q9WTK8-4] ENSMUST00000109126; ENSMUSP00000104754; ENSMUSG00000005883 [Q9WTK8-3] |
GeneIDi | 26972 |
KEGGi | mmu:26972 |
UCSCi | uc008odd.1, mouse [Q9WTK8-1] uc008ode.1, mouse [Q9WTK8-4] uc008odf.1, mouse [Q9WTK8-3] uc012ckq.1, mouse [Q9WTK8-5] |
Organism-specific databases
CTDi | 23626 |
MGIi | MGI:1349669, Spo11 |
Phylogenomic databases
eggNOGi | KOG2795, Eukaryota |
GeneTreei | ENSGT00390000001787 |
HOGENOMi | CLU_037229_1_1_1 |
InParanoidi | Q9WTK8 |
OMAi | FTDGDPY |
OrthoDBi | 1272299at2759 |
PhylomeDBi | Q9WTK8 |
TreeFami | TF314157 |
Miscellaneous databases
BioGRID-ORCSi | 26972, 0 hits in 17 CRISPR screens |
PROi | PR:Q9WTK8 |
RNActi | Q9WTK8, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000005883, Expressed in spermatocyte and 60 other tissues |
Genevisiblei | Q9WTK8, MM |
Family and domain databases
CDDi | cd00223, TOPRIM_TopoIIB_SPO, 1 hit |
Gene3Di | 1.10.10.10, 1 hit |
InterProi | View protein in InterPro IPR004084, Meiosis_Spo11 IPR013048, Meiotic_Spo11 IPR002815, Spo11/TopoVI_A IPR013049, Spo11/TopoVI_A_N IPR036078, Spo11/TopoVI_A_sf IPR034136, TOPRIM_Topo6A/Spo11 IPR036388, WH-like_DNA-bd_sf |
PANTHERi | PTHR10848, PTHR10848, 1 hit |
Pfami | View protein in Pfam PF03533, SPO11_like, 1 hit PF04406, TP6A_N, 1 hit |
PRINTSi | PR01551, SPO11HOMOLOG PR01550, TOP6AFAMILY |
SUPFAMi | SSF56726, SSF56726, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SPO11_MOUSE | |
Accessioni | Q9WTK8Primary (citable) accession number: Q9WTK8 Secondary accession number(s): Q8VIH6 Q9QZS1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2000 |
Last sequence update: | April 23, 2003 | |
Last modified: | February 10, 2021 | |
This is version 163 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families