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UniProtKB - Q9WTK3 (GPAA1_MOUSE)
Protein
Glycosylphosphatidylinositol anchor attachment 1 protein
Gene
Gpaa1
Organism
Mus musculus (Mouse)
Status
Functioni
Essential for GPI-anchoring of precursor proteins but not for GPI synthesis. Acts before or during formation of the carbonyl intermediate.
2 Publications: glycosylphosphatidylinositol-anchor biosynthesis Pathwayi
This protein is involved in the pathway glycosylphosphatidylinositol-anchor biosynthesis, which is part of Glycolipid biosynthesis.View all proteins of this organism that are known to be involved in the pathway glycosylphosphatidylinositol-anchor biosynthesis and in Glycolipid biosynthesis.
GO - Molecular functioni
- GPI anchor binding Source: Ensembl
- GPI-anchor transamidase activity Source: MGI
GO - Biological processi
- attachment of GPI anchor to protein Source: GO_Central
- GPI anchor biosynthetic process Source: MGI
- protein-containing complex assembly Source: MGI
Keywordsi
Biological process | GPI-anchor biosynthesis |
Enzyme and pathway databases
Reactomei | R-MMU-162791, Attachment of GPI anchor to uPAR |
UniPathwayi | UPA00196 |
Names & Taxonomyi
Protein namesi | Recommended name: Glycosylphosphatidylinositol anchor attachment 1 proteinShort name: GPI anchor attachment protein 1 Alternative name(s): GAA1 protein homolog Short name: mGAA1 |
Gene namesi | Name:Gpaa1 Synonyms:Gaa1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1202392, Gpaa1 |
VEuPathDBi | HostDB:ENSMUSG00000022561 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane By similarity; Multi-pass membrane protein By similarity
Cytoskeleton
- centrosome Source: MGI
Cytosol
- cytosol Source: MGI
Endoplasmic reticulum
- endoplasmic reticulum Source: MGI
- GPI-anchor transamidase complex Source: UniProtKB
Mitochondrion
- mitochondrion Source: MGI
Plasma Membrane
- integral component of plasma membrane Source: MGI
Other locations
- integral component of membrane Source: MGI
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 23 | CytoplasmicSequence analysisAdd BLAST | 23 | |
Transmembranei | 24 – 44 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 45 – 367 | LumenalSequence analysisAdd BLAST | 323 | |
Transmembranei | 368 – 388 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 389 – 426 | CytoplasmicSequence analysisAdd BLAST | 38 | |
Transmembranei | 427 – 447 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 448 – 457 | LumenalSequence analysis | 10 | |
Transmembranei | 458 – 478 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 479 – 499 | CytoplasmicSequence analysisAdd BLAST | 21 | |
Transmembranei | 500 – 520 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 521 – 542 | LumenalSequence analysisAdd BLAST | 22 | |
Transmembranei | 543 – 563 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 564 – 598 | CytoplasmicSequence analysisAdd BLAST | 35 | |
Transmembranei | 599 – 619 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 620 – 621 | LumenalSequence analysis | 2 |
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000087555 | 1 – 621 | Glycosylphosphatidylinositol anchor attachment 1 proteinAdd BLAST | 621 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 203 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
GlycoproteinProteomic databases
EPDi | Q9WTK3 |
MaxQBi | Q9WTK3 |
PaxDbi | Q9WTK3 |
PeptideAtlasi | Q9WTK3 |
PRIDEi | Q9WTK3 |
ProteomicsDBi | 271139 |
PTM databases
GlyGeni | Q9WTK3, 1 site |
PhosphoSitePlusi | Q9WTK3 |
Expressioni
Tissue specificityi
Ubiquitously expressed in fetal and adult tissues. Expressed at higher levels in fetal tissues than adult tissues. In embryos abundant in the choroid plexus, skeletal muscle,.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000022561, Expressed in hindbrain and 255 other tissues |
ExpressionAtlasi | Q9WTK3, baseline and differential |
Genevisiblei | Q9WTK3, MM |
Interactioni
Subunit structurei
Forms a complex with PIGK/GPI8, PIGT, PIGU and PIGS.
1 PublicationProtein-protein interaction databases
BioGRIDi | 200010, 2 interactors |
STRINGi | 10090.ENSMUSP00000023221 |
Miscellaneous databases
RNActi | Q9WTK3, protein |
Structurei
Family & Domainsi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3566, Eukaryota |
GeneTreei | ENSGT00390000013685 |
HOGENOMi | CLU_007442_2_1_1 |
InParanoidi | Q9WTK3 |
OMAi | ARRKNYW |
OrthoDBi | 1486030at2759 |
PhylomeDBi | Q9WTK3 |
TreeFami | TF313030 |
Family and domain databases
InterProi | View protein in InterPro IPR007246, Gaa1 |
PANTHERi | PTHR13304, PTHR13304, 1 hit |
Pfami | View protein in Pfam PF04114, Gaa1, 1 hit |
PIRSFi | PIRSF036762, GAA1, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All
Q9WTK3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGLLSDPVRR RALARIVLRL NTPLCVLSYV AGIAWFLALA FPPLTQRTYM
60 70 80 90 100
SENAMGSTMV EEQFVGGDRA RSFARDFAAH RKKPGALPVA WLERSMRSVG
110 120 130 140 150
LEVYTQSFSR KLPFPDETHE RYMVSGTNVY GILRAPRSAS TESLVLTVPC
160 170 180 190 200
GPDATNSQAV GLLLALAAHF RGQIYWAKDI IFLVTDHDLL GTEAWLEAYH
210 220 230 240 250
DINVTGIQSS PLQGRAGAIQ AAVALELSSD VVTSLDVTVE GLNGQLPNLD
260 270 280 290 300
LLNLFQTFCQ KGGLLCTLQG KLQPQDWTSL EGPLQGLQTL LLMVLRQASG
310 320 330 340 350
RPHGPHGLFL RYGVEALTLR GINSFRQYKY DLATVGKALE GMFRKLNHLL
360 370 380 390 400
ERLHQSFFFY LLPALSRFVS IGLYMPATGF LLLVLGLKAL ELWMQLHQAG
410 420 430 440 450
VNPEEAGKAP SPGTPLLPTQ GVGLASLTAP LLISQAMGLA LYFLPVLGQH
460 470 480 490 500
LATQHFPVAE AEAVVLTLLA IYVAGLALPH NTHRVVNSQV PDRGWMALKL
510 520 530 540 550
VALIYLALQL GCIALLNFSL GFLLAATMVP AAALAKPHGP RTLYAALLVV
560 570 580 590 600
TSPAVTLFGS LFLWRELLEV PLSLAEGWQL FLTALAQGVL EHYTYGALLF
610 620
PILALGLYPC WLLFWNVLFW K
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE9PW03 | E9PW03_MOUSE | Glycosylphosphatidylinositol anchor... | Gpaa1 | 561 | Annotation score: | ||
E9QA52 | E9QA52_MOUSE | Glycosylphosphatidylinositol anchor... | Gpaa1 | 74 | Annotation score: | ||
F6T7Y8 | F6T7Y8_MOUSE | Glycosylphosphatidylinositol anchor... | Gpaa1 | 102 | Annotation score: | ||
E9Q4I5 | E9Q4I5_MOUSE | Glycosylphosphatidylinositol anchor... | Gpaa1 | 74 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB002136 mRNA Translation: BAA82589.1 AB002138 Genomic DNA Translation: BAA82591.1 AB006970 mRNA Translation: BAB03274.1 AB006971 Genomic DNA Translation: BAB03275.1 AB017266 Genomic DNA Translation: BAA82586.1 AK077518 mRNA Translation: BAC36840.1 AK164219 mRNA Translation: BAE37686.1 AK164978 mRNA Translation: BAE37988.1 BC006697 mRNA Translation: AAH06697.1 |
CCDSi | CCDS37120.1 |
RefSeqi | NP_034461.1, NM_010331.2 |
Genome annotation databases
Ensembli | ENSMUST00000023221; ENSMUSP00000023221; ENSMUSG00000022561 |
GeneIDi | 14731 |
KEGGi | mmu:14731 |
UCSCi | uc007wjq.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB002136 mRNA Translation: BAA82589.1 AB002138 Genomic DNA Translation: BAA82591.1 AB006970 mRNA Translation: BAB03274.1 AB006971 Genomic DNA Translation: BAB03275.1 AB017266 Genomic DNA Translation: BAA82586.1 AK077518 mRNA Translation: BAC36840.1 AK164219 mRNA Translation: BAE37686.1 AK164978 mRNA Translation: BAE37988.1 BC006697 mRNA Translation: AAH06697.1 |
CCDSi | CCDS37120.1 |
RefSeqi | NP_034461.1, NM_010331.2 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 200010, 2 interactors |
STRINGi | 10090.ENSMUSP00000023221 |
PTM databases
GlyGeni | Q9WTK3, 1 site |
PhosphoSitePlusi | Q9WTK3 |
Proteomic databases
EPDi | Q9WTK3 |
MaxQBi | Q9WTK3 |
PaxDbi | Q9WTK3 |
PeptideAtlasi | Q9WTK3 |
PRIDEi | Q9WTK3 |
ProteomicsDBi | 271139 |
Protocols and materials databases
Antibodypediai | 28266, 153 antibodies from 24 providers |
DNASUi | 14731 |
Genome annotation databases
Ensembli | ENSMUST00000023221; ENSMUSP00000023221; ENSMUSG00000022561 |
GeneIDi | 14731 |
KEGGi | mmu:14731 |
UCSCi | uc007wjq.1, mouse |
Organism-specific databases
CTDi | 8733 |
MGIi | MGI:1202392, Gpaa1 |
VEuPathDBi | HostDB:ENSMUSG00000022561 |
Phylogenomic databases
eggNOGi | KOG3566, Eukaryota |
GeneTreei | ENSGT00390000013685 |
HOGENOMi | CLU_007442_2_1_1 |
InParanoidi | Q9WTK3 |
OMAi | ARRKNYW |
OrthoDBi | 1486030at2759 |
PhylomeDBi | Q9WTK3 |
TreeFami | TF313030 |
Enzyme and pathway databases
UniPathwayi | UPA00196 |
Reactomei | R-MMU-162791, Attachment of GPI anchor to uPAR |
Miscellaneous databases
BioGRID-ORCSi | 14731, 19 hits in 68 CRISPR screens |
PROi | PR:Q9WTK3 |
RNActi | Q9WTK3, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000022561, Expressed in hindbrain and 255 other tissues |
ExpressionAtlasi | Q9WTK3, baseline and differential |
Genevisiblei | Q9WTK3, MM |
Family and domain databases
InterProi | View protein in InterPro IPR007246, Gaa1 |
PANTHERi | PTHR13304, PTHR13304, 1 hit |
Pfami | View protein in Pfam PF04114, Gaa1, 1 hit |
PIRSFi | PIRSF036762, GAA1, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | GPAA1_MOUSE | |
Accessioni | Q9WTK3Primary (citable) accession number: Q9WTK3 Secondary accession number(s): Q3TNU9, Q9R1U8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 1, 2004 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 23, 2022 | |
This is version 144 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways