Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Mothers against decapentaplegic homolog 5

Gene

smad5

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Involved in ventralization. May mediate Bmp2b signaling during early phases of embryonic dorsal-ventral pattern formation. Required for initiation of Smad1 expression during gastrulation.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi65ZincBy similarity1
Metal bindingi110ZincBy similarity1
Metal bindingi122ZincBy similarity1
Metal bindingi127ZincBy similarity1

GO - Molecular functioni

  • DNA-binding transcription factor activity Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW
  • protein heterodimerization activity Source: ZFIN
  • transcription regulatory region DNA binding Source: ZFIN

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein, DNA-binding
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-DRE-201451 Signaling by BMP
R-DRE-5689880 Ub-specific processing proteases
R-DRE-8941326 RUNX2 regulates bone development

Names & Taxonomyi

Protein namesi
Recommended name:
Mothers against decapentaplegic homolog 5
Short name:
MAD homolog 5
Short name:
Mothers against DPP homolog 5
Alternative name(s):
Protein somitabun
SMAD family member 5
Short name:
SMAD 5
Short name:
Smad5
Gene namesi
Name:smad5
Synonyms:madh5, sbn
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Chromosome 14

Organism-specific databases

ZFINiZDB-GENE-990603-9 smad5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000908681 – 464Mothers against decapentaplegic homolog 5Add BLAST464

Proteomic databases

PaxDbiQ9W7E7

Expressioni

Developmental stagei

Expressed both maternally and zygotically. Uniform distribution at the cleavage, blastula, gastrula and early somitogenesis stages. Levels decline during the early gastrula stages. At 26 hours, still expressed in the head, forming gut and dorsal neural tube.2 Publications

Gene expression databases

BgeeiENSDARG00000037238 Expressed in 31 organ(s), highest expression level in mature ovarian follicle
ExpressionAtlasiQ9W7E7 baseline and differential

Interactioni

Subunit structurei

May form trimers with the co-SMAD SMAD4.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi7955.ENSDARP00000054174

Structurei

3D structure databases

ProteinModelPortaliQ9W7E7
SMRiQ9W7E7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini13 – 137MH1PROSITE-ProRule annotationAdd BLAST125
Domaini270 – 464MH2PROSITE-ProRule annotationAdd BLAST195

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi40 – 46Poly-Lys7
Compositional biasi187 – 231Pro-richAdd BLAST45

Sequence similaritiesi

Belongs to the dwarfin/SMAD family.Curated

Phylogenomic databases

eggNOGiKOG3701 Eukaryota
ENOG410XQKU LUCA
GeneTreeiENSGT00760000119091
HOGENOMiHOG000286018
HOVERGENiHBG053353
InParanoidiQ9W7E7
KOiK16790
OMAiDVQPVEY
OrthoDBiEOG091G082C
PhylomeDBiQ9W7E7
TreeFamiTF314923

Family and domain databases

Gene3Di2.60.200.10, 1 hit
3.90.520.10, 1 hit
InterProiView protein in InterPro
IPR013790 Dwarfin
IPR003619 MAD_homology1_Dwarfin-type
IPR013019 MAD_homology_MH1
IPR017855 SMAD-like_dom_sf
IPR001132 SMAD_dom_Dwarfin-type
IPR008984 SMAD_FHA_dom_sf
IPR036578 SMAD_MH1_sf
PANTHERiPTHR13703 PTHR13703, 1 hit
PfamiView protein in Pfam
PF03165 MH1, 1 hit
PF03166 MH2, 1 hit
SMARTiView protein in SMART
SM00523 DWA, 1 hit
SM00524 DWB, 1 hit
SUPFAMiSSF49879 SSF49879, 1 hit
SSF56366 SSF56366, 1 hit
PROSITEiView protein in PROSITE
PS51075 MH1, 1 hit
PS51076 MH2, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.iShow all

Q9W7E7-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTSMSSLFSF TSPAVKRLLG WKQGDEEEKW AEKAVDALVK KLKKKKGAME
60 70 80 90 100
DLEKALSSPG QPSKCVTIPR SLDGRLQVSH RKGLPHVIYC RVWRWPDLQS
110 120 130 140 150
HHELKPLEVC EYPFGSKQKE VCINPYHYKR VESPVLPPVL VPRHSEFNPQ
160 170 180 190 200
HSLLVQFRNL SHNEPHMPLN ATFPESFQQH SGGSSFPISP NSPYPPSPAS
210 220 230 240 250
SGTYPNSPAS SGPSSPFQLP ADTPPPAYMP PDEQMGQDGS QSMETGSSLA
260 270 280 290 300
PQNMPRGDVQ PVEYQEPSHW CSIVYYELNN RVGEAYHASS TSVLVDGFTD
310 320 330 340 350
PSNNKNRFCL GLLSNVNRNS TIENTRRHIG KGVHLYYVGG EVYAECLSDT
360 370 380 390 400
SIFVQSRNCN YHHGFHPTTV CKIPSGCSLK IFNNQEFAQL LAQSVNHGFE
410 420 430 440 450
AVYELTKMCT IRMSFVKGWG AEYHRQDVTS TPCWIEVHLH GPLQWLDKVL
460
TQMGSPLNPI SSVS
Length:464
Mass (Da):51,861
Last modified:November 1, 1999 - v1
Checksum:i5018387FBA4072DC
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A5PMB9A5PMB9_DANRE
Mothers against decapentaplegic hom...
smad5
131Annotation score:

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti429T → I in allele DTC24; dorsalized embryos. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF127920 mRNA Translation: AAD43904.1
AF168775 mRNA Translation: AAF06738.1
BC065644 mRNA Translation: AAH65644.1
RefSeqiNP_001333238.1, NM_001346309.1
NP_571443.3, NM_131368.3
XP_005173221.1, XM_005173164.3
XP_009289292.1, XM_009291017.2
UniGeneiDr.75335

Genome annotation databases

EnsembliENSDART00000054175; ENSDARP00000054174; ENSDARG00000037238
ENSDART00000183347; ENSDARP00000155132; ENSDARG00000037238
ENSDART00000187280; ENSDARP00000153815; ENSDARG00000037238
ENSDART00000191084; ENSDARP00000149901; ENSDARG00000037238
ENSDART00000191143; ENSDARP00000154529; ENSDARG00000037238
GeneIDi30641
KEGGidre:30641

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiSMAD5_DANRE
AccessioniPrimary (citable) accession number: Q9W7E7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 27, 2002
Last sequence update: November 1, 1999
Last modified: September 12, 2018
This is version 141 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again