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Protein

Hypoxanthine-guanine phosphoribosyltransferase

Gene

HPRT1

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5-phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway (By similarity).By similarity

Catalytic activityi

IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate.
GMP + diphosphate = guanine + 5-phospho-alpha-D-ribose 1-diphosphate.

Cofactori

Mg2+By similarityNote: Binds 2 magnesium ions per subunit. The magnesium ions are essentially bound to the substrate and have few direct interactions with the protein.By similarity

Pathwayi: IMP biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes IMP from hypoxanthine.
Proteins known to be involved in this subpathway in this organism are:
  1. Hypoxanthine-guanine phosphoribosyltransferase (HPRT1), Hypoxanthine phosphoribosyltransferase (PRTFDC1), Hypoxanthine phosphoribosyltransferase (HPRT1), Hypoxanthine phosphoribosyltransferase (HPRT1), Hypoxanthine phosphoribosyltransferase (HPRTL)
This subpathway is part of the pathway IMP biosynthesis via salvage pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes IMP from hypoxanthine, the pathway IMP biosynthesis via salvage pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei69GMPBy similarity1
Active sitei138Proton acceptorBy similarity1
Binding sitei166GMPBy similarity1
Metal bindingi194MagnesiumBy similarity1
Binding sitei194GMP; via carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi134 – 142GMPBy similarity9
Nucleotide bindingi186 – 188GMPBy similarity3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processPurine salvage
LigandMagnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-GGA-74217 Purine salvage
UniPathwayi
UPA00591;UER00648

Names & Taxonomyi

Protein namesi
Recommended name:
Hypoxanthine-guanine phosphoribosyltransferase (EC:2.4.2.8)
Short name:
HGPRT
Short name:
HGPRTase
Gene namesi
Name:HPRT1
Synonyms:HPRT
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Chromosome 4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001395921 – 218Hypoxanthine-guanine phosphoribosyltransferaseAdd BLAST218

Proteomic databases

PaxDbiQ9W719
PRIDEiQ9W719

Expressioni

Gene expression databases

ExpressionAtlasiQ9W719 baseline and differential

Interactioni

Subunit structurei

Homotetramer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9031.ENSGALP00000031748

Structurei

3D structure databases

ProteinModelPortaliQ9W719
SMRiQ9W719
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3367 Eukaryota
COG0634 LUCA
GeneTreeiENSGT00390000017323
HOGENOMiHOG000236521
HOVERGENiHBG000242
InParanoidiQ9W719
KOiK00760
PhylomeDBiQ9W719

Family and domain databases

CDDicd06223 PRTases_typeI, 1 hit
InterProiView protein in InterPro
IPR005904 Hxn_phspho_trans
IPR000836 PRibTrfase_dom
IPR029057 PRTase-like
PfamiView protein in Pfam
PF00156 Pribosyltran, 1 hit
SUPFAMiSSF53271 SSF53271, 1 hit
TIGRFAMsiTIGR01203 HGPRTase, 1 hit
PROSITEiView protein in PROSITE
PS00103 PUR_PYR_PR_TRANSFER, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

Q9W719-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MATHSPCIVI GDDEQGYDLD LFCIPKHYAD DLEKVYIPHG LIMDRTERLA
60 70 80 90 100
REIMKGMGGH HIVALCVLKG GYKFFADLLD YIKALNRNSD KSIPMTVDFI
110 120 130 140 150
RLKSYCNDQS TGDIKVIGGD DLSTLTGKNV LIVEDIIDTG KTMKTLLSLL
160 170 180 190 200
KQYNPKMVKV ASLLVKRTPR SVGYRPDFVG FEVPDKFVVG YALDYNEYFR
210
DLNHICVISE TGKQKYKA
Length:218
Mass (Da):24,609
Last modified:November 1, 1999 - v1
Checksum:i2228B84B0B6AF727
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F1NBZ6F1NBZ6_CHICK
Hypoxanthine phosphoribosyltransfer...
HPRT1
218Annotation score:
A0A1D5PEZ7A0A1D5PEZ7_CHICK
Hypoxanthine phosphoribosyltransfer...
HPRT1
212Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ132697 mRNA Translation: CAB46657.1
RefSeqiNP_990179.1, NM_204848.1
UniGeneiGga.3545

Genome annotation databases

EnsembliENSGALT00000009843; ENSGALP00000009829; ENSGALG00000006098
GeneIDi395653
KEGGigga:395653

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ132697 mRNA Translation: CAB46657.1
RefSeqiNP_990179.1, NM_204848.1
UniGeneiGga.3545

3D structure databases

ProteinModelPortaliQ9W719
SMRiQ9W719
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9031.ENSGALP00000031748

Proteomic databases

PaxDbiQ9W719
PRIDEiQ9W719

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSGALT00000009843; ENSGALP00000009829; ENSGALG00000006098
GeneIDi395653
KEGGigga:395653

Organism-specific databases

CTDi3251

Phylogenomic databases

eggNOGiKOG3367 Eukaryota
COG0634 LUCA
GeneTreeiENSGT00390000017323
HOGENOMiHOG000236521
HOVERGENiHBG000242
InParanoidiQ9W719
KOiK00760
PhylomeDBiQ9W719

Enzyme and pathway databases

UniPathwayi
UPA00591;UER00648

ReactomeiR-GGA-74217 Purine salvage

Miscellaneous databases

PROiPR:Q9W719

Gene expression databases

ExpressionAtlasiQ9W719 baseline and differential

Family and domain databases

CDDicd06223 PRTases_typeI, 1 hit
InterProiView protein in InterPro
IPR005904 Hxn_phspho_trans
IPR000836 PRibTrfase_dom
IPR029057 PRTase-like
PfamiView protein in Pfam
PF00156 Pribosyltran, 1 hit
SUPFAMiSSF53271 SSF53271, 1 hit
TIGRFAMsiTIGR01203 HGPRTase, 1 hit
PROSITEiView protein in PROSITE
PS00103 PUR_PYR_PR_TRANSFER, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiHPRT_CHICK
AccessioniPrimary (citable) accession number: Q9W719
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: November 1, 1999
Last modified: November 7, 2018
This is version 106 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health

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