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UniProtKB - Q9W265 (HOT_DROME)
Protein
Probable hydroxyacid-oxoacid transhydrogenase, mitochondrial
Gene
T3dh
Organism
Drosophila melanogaster (Fruit fly)
Status
Functioni
Catalyzes the cofactor-independent reversible oxidation of gamma-hydroxybutyrate (GHB) to succinic semialdehyde (SSA) coupled to reduction of 2-ketoglutarate (2-KG) to D-2-hydroxyglutarate (D-2-HG). L-3-hydroxybutyrate (L-3-OHB) is also a substrate for HOT when using 2-KG as hydrogen acceptor, resulting in the formation of D-2-HG (By similarity).
By similarityCatalytic activityi
GO - Molecular functioni
- alcohol dehydrogenase (NAD+) activity Source: GO_Central
- hydroxyacid-oxoacid transhydrogenase activity Source: UniProtKB
- metal ion binding Source: InterPro
GO - Biological processi
- glutamate catabolic process via 2-oxoglutarate Source: UniProtKB
Keywordsi
Molecular function | Oxidoreductase |
Enzyme and pathway databases
Reactomei | R-DME-880009, Interconversion of 2-oxoglutarate and 2-hydroxyglutarate |
Names & Taxonomyi
Protein namesi | Recommended name: Probable hydroxyacid-oxoacid transhydrogenase, mitochondrial (EC:1.1.99.24)Short name: HOT |
Gene namesi | Name:T3dh ORF Names:CG3425 |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0017482, T3dh |
VEuPathDBi | VectorBase:FBgn0017482 |
Subcellular locationi
Mitochondrion
- Mitochondrion By similarity
Mitochondrion
- mitochondrion Source: UniProtKB
Keywords - Cellular componenti
MitochondrionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – ? | MitochondrionSequence analysis | ||
ChainiPRO_0000323005 | ? – 464 | Probable hydroxyacid-oxoacid transhydrogenase, mitochondrial |
Proteomic databases
PaxDbi | Q9W265 |
Expressioni
Gene expression databases
Bgeei | FBgn0017482, Expressed in arthropod fat body and 17 other tissues |
ExpressionAtlasi | Q9W265, baseline and differential |
Genevisiblei | Q9W265, DM |
Family & Domainsi
Sequence similaritiesi
Belongs to the iron-containing alcohol dehydrogenase family. Hydroxyacid-oxoacid transhydrogenase subfamily.Curated
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG3857, Eukaryota |
GeneTreei | ENSGT00390000003849 |
HOGENOMi | CLU_007207_0_7_1 |
InParanoidi | Q9W265 |
OMAi | IRFGPGC |
OrthoDBi | 776004at2759 |
PhylomeDBi | Q9W265 |
Family and domain databases
CDDi | cd08190, HOT, 1 hit |
InterProi | View protein in InterPro IPR001670, ADH_Fe/GldA IPR039697, Alcohol_dehydrogenase_Fe IPR042157, HOT |
PANTHERi | PTHR11496, PTHR11496, 1 hit |
Pfami | View protein in Pfam PF00465, Fe-ADH, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q9W265-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSRKNVLGLI NTIVANSCKC PAHSHNYGSA APTASQTGRM EYAFEMSAST
60 70 80 90 100
VRFGPGVSAE VGADLRNLGA RKVCLVTDKN VVQLPSVKVA LDSLARNGIN
110 120 130 140 150
YEVYDETRVE PTDGSMWHAV EFARGKEFDA FLAIGGGSAM DTAKAANLFS
160 170 180 190 200
SDANAEFLDY VNCPIGRGKE ISVKLKPLIA MPTTSGTGSE TTGVAIFDYK
210 220 230 240 250
KLHAKTGISS KFLKPTLAVI DPLHTLSQPQ RVMAFAGFDV FCHALESFTA
260 270 280 290 300
VDYRERGLAP SDPSLRPTYQ GRNPVSDVWA RFALETIRKN FVNAIYQPDN
310 320 330 340 350
LEARSQMHLA STMAGVGFGN AGVHLCHGLS YPISGNVRDY KPKGYSADHA
360 370 380 390 400
LIPHGLSVVI SAPAVFEFTA PACPDRHLEA AQLLGAEVRG VEKADAGRLL
410 420 430 440 450
ADTVRGFMQR AGIENGLREL GFSSSDIPAL VEGTLPQERI TKLAPRAQTQ
460
ENLSQLFEKS MEVY
Sequence cautioni
The sequence AAL28462 differs from that shown. Reason: Erroneous initiation.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 108 | R → L in AAM11277 (PubMed:12537569).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE013599 Genomic DNA Translation: AAF46832.1 AY060914 mRNA Translation: AAL28462.1 Different initiation. AY094924 mRNA Translation: AAM11277.1 |
RefSeqi | NP_477209.2, NM_057861.3 |
Genome annotation databases
EnsemblMetazoai | FBtr0071848; FBpp0071759; FBgn0017482 |
GeneIDi | 37551 |
KEGGi | dme:Dmel_CG3425 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE013599 Genomic DNA Translation: AAF46832.1 AY060914 mRNA Translation: AAL28462.1 Different initiation. AY094924 mRNA Translation: AAM11277.1 |
RefSeqi | NP_477209.2, NM_057861.3 |
3D structure databases
AlphaFoldDBi | Q9W265 |
SMRi | Q9W265 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 7227.FBpp0071759 |
Proteomic databases
PaxDbi | Q9W265 |
Protocols and materials databases
DNASUi | 37551 |
Genome annotation databases
EnsemblMetazoai | FBtr0071848; FBpp0071759; FBgn0017482 |
GeneIDi | 37551 |
KEGGi | dme:Dmel_CG3425 |
Organism-specific databases
CTDi | 37551 |
FlyBasei | FBgn0017482, T3dh |
VEuPathDBi | VectorBase:FBgn0017482 |
Phylogenomic databases
eggNOGi | KOG3857, Eukaryota |
GeneTreei | ENSGT00390000003849 |
HOGENOMi | CLU_007207_0_7_1 |
InParanoidi | Q9W265 |
OMAi | IRFGPGC |
OrthoDBi | 776004at2759 |
PhylomeDBi | Q9W265 |
Enzyme and pathway databases
Reactomei | R-DME-880009, Interconversion of 2-oxoglutarate and 2-hydroxyglutarate |
Miscellaneous databases
BioGRID-ORCSi | 37551, 0 hits in 1 CRISPR screen |
ChiTaRSi | T3dh, fly |
GenomeRNAii | 37551 |
PROi | PR:Q9W265 |
Gene expression databases
Bgeei | FBgn0017482, Expressed in arthropod fat body and 17 other tissues |
ExpressionAtlasi | Q9W265, baseline and differential |
Genevisiblei | Q9W265, DM |
Family and domain databases
CDDi | cd08190, HOT, 1 hit |
InterProi | View protein in InterPro IPR001670, ADH_Fe/GldA IPR039697, Alcohol_dehydrogenase_Fe IPR042157, HOT |
PANTHERi | PTHR11496, PTHR11496, 1 hit |
Pfami | View protein in Pfam PF00465, Fe-ADH, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | HOT_DROME | |
Accessioni | Q9W265Primary (citable) accession number: Q9W265 Secondary accession number(s): Q8SX03, Q95S86 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 18, 2008 |
Last sequence update: | May 1, 2000 | |
Last modified: | May 25, 2022 | |
This is version 132 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - SIMILARITY comments
Index of protein domains and families