UniProtKB - Q9W252 (RAD50_DROME)
Protein
DNA repair protein RAD50
Gene
rad50
Organism
Drosophila melanogaster (Fruit fly)
Status
Functioni
Essential component of the MRN complex, a complex that possesses single-stranded DNA endonuclease and 3' to 5' exonuclease activities, and plays a central role in double-strand break (DSB) repair. The complex participates in processes such as DNA recombination, DNA repair, genome stability, telomere integrity and meiosis. The MRN complex may protect telomeres by facilitating recruitment of HOAP and HP1 at chromosome ends. In the complex, it mediates the ATP-binding and is probably required to bind DNA ends and hold them in close proximity.2 Publications
Cofactori
Zn2+By similarityNote: Binds 1 zinc ion per homodimer.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 689 | ZincPROSITE-ProRule annotation | 1 | |
Metal bindingi | 692 | ZincPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 36 – 43 | ATPSequence analysis | 8 |
GO - Molecular functioni
- adenylate kinase activity Source: GO_Central
- ATPase activity Source: InterPro
- ATP binding Source: UniProtKB-KW
- double-stranded DNA binding Source: UniProtKB
- double-stranded telomeric DNA binding Source: GO_Central
- G-quadruplex DNA binding Source: GO_Central
- metal ion binding Source: UniProtKB-KW
- single-stranded telomeric DNA binding Source: GO_Central
GO - Biological processi
- chromosome organization Source: FlyBase
- chromosome organization involved in meiotic cell cycle Source: GO_Central
- DNA duplex unwinding Source: GO_Central
- double-strand break repair Source: UniProtKB
- nucleic acid phosphodiester bond hydrolysis Source: GO_Central
- protein localization Source: UniProtKB
- telomere capping Source: FlyBase
- telomere maintenance Source: FlyBase
- telomere maintenance via recombination Source: UniProtKB
- telomere maintenance via telomerase Source: GO_Central
Keywordsi
Molecular function | Hydrolase |
Biological process | Cell cycle, DNA damage, DNA repair, Meiosis |
Ligand | ATP-binding, Metal-binding, Nucleotide-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-DME-2559586, DNA Damage/Telomere Stress Induced Senescence R-DME-5685939, HDR through MMEJ (alt-NHEJ) R-DME-5693548, Sensing of DNA Double Strand Breaks R-DME-5693565, Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks R-DME-5693607, Processing of DNA double-strand break ends |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:rad50 ORF Names:CG6339 |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Holometabola › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0034728, rad50 |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
Other locations
- Chromosome 1 Publication
- telomere 1 Publication
Note: Uniformly distributed along mitotic chromosomes.
Nucleus
- condensed nuclear chromosome Source: GO_Central
- Mre11 complex Source: GO_Central
- nuclear chromatin Source: GO_Central
- nuclear chromosome, telomeric region Source: GO_Central
- nucleus Source: FlyBase
Other locations
- condensed chromosome Source: UniProtKB
- site of double-strand break Source: GO_Central
Keywords - Cellular componenti
Chromosome, Nucleus, TelomerePathology & Biotechi
Disruption phenotypei
Death during pupal stage, possibly due to the accumulation of DNA DSBs and the induction of apoptosis in third instar larvae.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000138645 | 1 – 1318 | DNA repair protein RAD50Add BLAST | 1318 |
Proteomic databases
PaxDbi | Q9W252 |
Expressioni
Gene expression databases
Bgeei | FBgn0034728, Expressed in female gonad and 50 other tissues |
ExpressionAtlasi | Q9W252, baseline and differential |
Genevisiblei | Q9W252, DM |
Interactioni
Subunit structurei
Homodimer. Probable component of the MRN complex with mre11 (By similarity).
By similarityProtein-protein interaction databases
BioGRIDi | 63180, 15 interactors |
IntActi | Q9W252, 5 interactors |
STRINGi | 7227.FBpp0290882 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 645 – 741 | Zinc-hookPROSITE-ProRule annotationAdd BLAST | 97 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 189 – 256 | Sequence analysisAdd BLAST | 68 | |
Coiled coili | 305 – 333 | Sequence analysisAdd BLAST | 29 | |
Coiled coili | 434 – 641 | Sequence analysisAdd BLAST | 208 | |
Coiled coili | 645 – 685 | Sequence analysisAdd BLAST | 41 | |
Coiled coili | 713 – 741 | Sequence analysisAdd BLAST | 29 | |
Coiled coili | 830 – 1101 | Sequence analysisAdd BLAST | 272 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 1208 – 1245 | Ala/Asp-rich (DA-box)Add BLAST | 38 |
Domaini
The zinc-hook, which separates the large intramolecular coiled coil regions, contains 2 Cys residues that coordinate one molecule of zinc with the help of the 2 Cys residues of the zinc-hook of another RAD50 molecule, thereby forming a V-shaped homodimer. The two heads of the homodimer, which constitute the ATP-binding domain, interact with the MRE11 homodimer (By similarity).By similarity
Sequence similaritiesi
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | KOG0962, Eukaryota |
GeneTreei | ENSGT00390000018781 |
InParanoidi | Q9W252 |
OMAi | CFGVNCG |
OrthoDBi | 179362at2759 |
PhylomeDBi | Q9W252 |
Family and domain databases
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR038729, Rad50/SbcC_AAA IPR004584, Rad50_eukaryotes IPR013134, Zn_hook_RAD50 |
Pfami | View protein in Pfam PF13476, AAA_23, 1 hit PF04423, Rad50_zn_hook, 1 hit |
SUPFAMi | SSF52540, SSF52540, 3 hits |
TIGRFAMsi | TIGR00606, rad50, 1 hit |
PROSITEi | View protein in PROSITE PS51131, ZN_HOOK, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q9W252-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSSIESLSIQ GIRSFGTYAD DLQSIKFSSP VTLILGENGC GKTTVVECLK
60 70 80 90 100
YALTGECPPG SDRGKSFVHD PKIFGLNEVL AQIKMQVRDR RGAQVSICRT
110 120 130 140 150
MKVSKKRNKM SFETMDSTIN FLTGAGQSKR EKQDSLSGRS VDIDVAISDF
160 170 180 190 200
MGVSKAIINN VLFCHQEDSS WPLDESKKLK EKFDAIFGIT EYNKALDKII
210 220 230 240 250
KLRKEAMEEL KIKEANIKHV AYLKQEMEVK TLNLQKAQRK CDAIKAQCSE
260 270 280 290 300
CEEEMKPIEA RLVEIRNVEF EIGKYQAQKV EMDTKHKNCK DQISTLTLKI
310 320 330 340 350
KKPFRGTLDE LDQEISNFDQ RMLEMRQKRT EVEGDLSQIK RSSVAEQEKL
360 370 380 390 400
GTQDRKHCLA KQRHQSELAC RAQLLKRVKE FCRELHIPID CDLVEQPEKM
410 420 430 440 450
GEVLRDIEAM IITKHCEITE IVEQNEKADR SRQVKIDELR IELTKSEQSV
460 470 480 490 500
TAQEKQRESS KRESETLGVE IKKIETSMQD LKKLEKEINE VNELYESATK
510 520 530 540 550
NIDQQAIKDA IARKKASIAE NQIQFKKLDE QLTFLGSMAK LVAECSLKQK
560 570 580 590 600
ELDKKNQEVH RVRSRHSDHF GKLFKEPITC NYRRSMQVVY EKLRREIQEL
610 620 630 640 650
NEKANTQKLK EQSYEIKRKN LISDISRMEK ELKDSEELIY QKCRSTPYDD
660 670 680 690 700
LLERSKTTIS KLQFDHGALK SSEALYKKYI QKMDEEPSCP LCHHNMTSDE
710 720 730 740 750
ACDLTSELTD EIQKLPDNIT RAEKALKAEQ IKYENLLQLK PTILKVKELK
760 770 780 790 800
DSLPQKKEEL KKVEELLGDS VSEYETLIAL IGEPTHNMEL ANSMMGDMSL
810 820 830 840 850
LDEALKDSAR LTKDLDLQKG QLPASYDSSV SMDDLQAEKS KVSKELETER
860 870 880 890 900
KELESAQNAV QQQMDALNRL REKKNSLKDR QIHLREGLQS LPQLKERLEK
910 920 930 940 950
LNSFLTTVAS EISELKAKIQ PLKLNLRAAI EEKERLKKSE SEKLAQLNSK
960 970 980 990 1000
YNSYKSTDHD IQRLNKEAED YAKLDLRNEI KKLDEIIMAS KDKLRKLEAE
1010 1020 1030 1040 1050
ISLKTDELET IKTECSNQQT VERDLKDNRE LKQLEDKEAK LRESCQVLDK
1060 1070 1080 1090 1100
QLGNLDFHSV SKEKVNLTKQ RDKATVRKGE LLGQLGEIHS QVNKLQREID
1110 1120 1130 1140 1150
EPRFKESLKN FRKANYEIEV TRLCIEDLGQ YRLALEWALI QFHSEKMEMI
1160 1170 1180 1190 1200
NRLIREYWRK IYRGNDIDYI QVKTDEVSSD ASADRRKTYN YRVVQSKNYS
1210 1220 1230 1240 1250
EIEMRGRCSA GQRVLASLII RLALAETFSS NCGVLALDEP TTNLDRANIN
1260 1270 1280 1290 1300
SLCEALNCIV EERQSQSNFM LIIITHDENF VSSLGKITSY HRVFRNEECK
1310
SVIRRVEAGP SKKALIDQ
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A0B4K7L0 | A0A0B4K7L0_DROME | Rad50, isoform E | rad50 6339, Dmel\CG6339, Dmrad50, RAD50, Rad50 | 1,318 | Annotation score: |
Sequence cautioni
The sequence AAK93530 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
The sequence AAO39559 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 880 | R → K in AAK93530 (PubMed:12537569).Curated | 1 | |
Sequence conflicti | 959 | H → Q in AAK93530 (PubMed:12537569).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 208 | E → D in strain: NC335, NC362 and NC390. 1 Publication | 1 | |
Natural varianti | 219 | H → V in strain: NC335, NC362 and NC390. 1 Publication | 1 | |
Natural varianti | 220 | V → E in strain: NC335, NC362 and NC390. 1 Publication | 1 | |
Natural varianti | 246 | A → E in strain: MW25. 1 Publication | 1 | |
Natural varianti | 249 | S → M in strain: MW25. 1 Publication | 1 | |
Natural varianti | 281 | E → Q in strain: NC306. 1 Publication | 1 | |
Natural varianti | 349 | K → T in strain: MW9. 1 Publication | 1 | |
Natural varianti | 422 | V → E in strain: NC361 and NC375. 1 Publication | 1 | |
Natural varianti | 450 | V → I in strain: MW6. 1 Publication | 1 | |
Natural varianti | 480 | D → N in strain: MW6. 1 Publication | 1 | |
Natural varianti | 490 | E → Q in strain: MW25, MW27, MW56, MW9, NC303, NC306, NC335, NC336, NC390 and NC399. 1 Publication | 1 | |
Natural varianti | 491 | V → I in strain: NC303, NC306 and NC335. 1 Publication | 1 | |
Natural varianti | 580 | C → S in strain: MW25. 1 Publication | 1 | |
Natural varianti | 586 | M → I in strain: MW25. 1 Publication | 1 | |
Natural varianti | 623 | S → N in strain: NC336, NC358, NC361, NC362 and NC375. 1 Publication | 1 | |
Natural varianti | 646 | T → S in strain: NC357 and NC397. 1 Publication | 1 | |
Natural varianti | 657 | T → A in strain: MW6, NC357, NC358, NC361, NC362, NC375 and NC397. 1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE013599 Genomic DNA Translation: AAF46847.3 BT003555 mRNA Translation: AAO39559.1 Different initiation. FJ219324 Genomic DNA Translation: ACI97309.1 FJ219329 Genomic DNA Translation: ACI97314.1 FJ219330 Genomic DNA Translation: ACI97315.1 FJ219331 Genomic DNA Translation: ACI97316.1 FJ219332 Genomic DNA Translation: ACI97317.1 FJ219333 Genomic DNA Translation: ACI97318.1 FJ219334 Genomic DNA Translation: ACI97319.1 FJ219335 Genomic DNA Translation: ACI97320.1 FJ219336 Genomic DNA Translation: ACI97321.1 FJ219337 Genomic DNA Translation: ACI97322.1 FJ219338 Genomic DNA Translation: ACI97323.1 FJ219339 Genomic DNA Translation: ACI97324.1 FJ219340 Genomic DNA Translation: ACI97325.1 FJ219341 Genomic DNA Translation: ACI97326.1 FJ219342 Genomic DNA Translation: ACI97327.1 FJ219343 Genomic DNA Translation: ACI97328.1 FJ219344 Genomic DNA Translation: ACI97329.1 FJ219345 Genomic DNA Translation: ACI97330.1 FJ219346 Genomic DNA Translation: ACI97331.1 FJ219347 Genomic DNA Translation: ACI97332.1 FJ219348 Genomic DNA Translation: ACI97333.1 FJ219349 Genomic DNA Translation: ACI97334.1 FJ219350 Genomic DNA Translation: ACI97335.1 AY052106 mRNA Translation: AAK93530.1 Different initiation. |
RefSeqi | NP_726199.3, NM_166533.4 |
Genome annotation databases
EnsemblMetazoai | FBtr0301668; FBpp0290882; FBgn0034728 |
GeneIDi | 37564 |
KEGGi | dme:Dmel_CG6339 |
UCSCi | CG6339-RB, d. melanogaster |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE013599 Genomic DNA Translation: AAF46847.3 BT003555 mRNA Translation: AAO39559.1 Different initiation. FJ219324 Genomic DNA Translation: ACI97309.1 FJ219329 Genomic DNA Translation: ACI97314.1 FJ219330 Genomic DNA Translation: ACI97315.1 FJ219331 Genomic DNA Translation: ACI97316.1 FJ219332 Genomic DNA Translation: ACI97317.1 FJ219333 Genomic DNA Translation: ACI97318.1 FJ219334 Genomic DNA Translation: ACI97319.1 FJ219335 Genomic DNA Translation: ACI97320.1 FJ219336 Genomic DNA Translation: ACI97321.1 FJ219337 Genomic DNA Translation: ACI97322.1 FJ219338 Genomic DNA Translation: ACI97323.1 FJ219339 Genomic DNA Translation: ACI97324.1 FJ219340 Genomic DNA Translation: ACI97325.1 FJ219341 Genomic DNA Translation: ACI97326.1 FJ219342 Genomic DNA Translation: ACI97327.1 FJ219343 Genomic DNA Translation: ACI97328.1 FJ219344 Genomic DNA Translation: ACI97329.1 FJ219345 Genomic DNA Translation: ACI97330.1 FJ219346 Genomic DNA Translation: ACI97331.1 FJ219347 Genomic DNA Translation: ACI97332.1 FJ219348 Genomic DNA Translation: ACI97333.1 FJ219349 Genomic DNA Translation: ACI97334.1 FJ219350 Genomic DNA Translation: ACI97335.1 AY052106 mRNA Translation: AAK93530.1 Different initiation. |
RefSeqi | NP_726199.3, NM_166533.4 |
3D structure databases
SMRi | Q9W252 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 63180, 15 interactors |
IntActi | Q9W252, 5 interactors |
STRINGi | 7227.FBpp0290882 |
Proteomic databases
PaxDbi | Q9W252 |
Genome annotation databases
EnsemblMetazoai | FBtr0301668; FBpp0290882; FBgn0034728 |
GeneIDi | 37564 |
KEGGi | dme:Dmel_CG6339 |
UCSCi | CG6339-RB, d. melanogaster |
Organism-specific databases
CTDi | 10111 |
FlyBasei | FBgn0034728, rad50 |
Phylogenomic databases
eggNOGi | KOG0962, Eukaryota |
GeneTreei | ENSGT00390000018781 |
InParanoidi | Q9W252 |
OMAi | CFGVNCG |
OrthoDBi | 179362at2759 |
PhylomeDBi | Q9W252 |
Enzyme and pathway databases
Reactomei | R-DME-2559586, DNA Damage/Telomere Stress Induced Senescence R-DME-5685939, HDR through MMEJ (alt-NHEJ) R-DME-5693548, Sensing of DNA Double Strand Breaks R-DME-5693565, Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks R-DME-5693607, Processing of DNA double-strand break ends |
Miscellaneous databases
BioGRID-ORCSi | 37564, 1 hit in 1 CRISPR screen |
GenomeRNAii | 37564 |
PROi | PR:Q9W252 |
Gene expression databases
Bgeei | FBgn0034728, Expressed in female gonad and 50 other tissues |
ExpressionAtlasi | Q9W252, baseline and differential |
Genevisiblei | Q9W252, DM |
Family and domain databases
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR038729, Rad50/SbcC_AAA IPR004584, Rad50_eukaryotes IPR013134, Zn_hook_RAD50 |
Pfami | View protein in Pfam PF13476, AAA_23, 1 hit PF04423, Rad50_zn_hook, 1 hit |
SUPFAMi | SSF52540, SSF52540, 3 hits |
TIGRFAMsi | TIGR00606, rad50, 1 hit |
PROSITEi | View protein in PROSITE PS51131, ZN_HOOK, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RAD50_DROME | |
Accessioni | Q9W252Primary (citable) accession number: Q9W252 Secondary accession number(s): B6UXS6 Q960E0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 2005 |
Last sequence update: | April 18, 2012 | |
Last modified: | December 2, 2020 | |
This is version 159 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - SIMILARITY comments
Index of protein domains and families