UniProtKB - Q9W0P8 (MED14_DROME)
Protein
Mediator of RNA polymerase II transcription subunit 14
Gene
MED14
Organism
Drosophila melanogaster (Fruit fly)
Status
Functioni
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors. Required for activated transcription of the MtnA, MtnB and MtnD genes.2 Publications
GO - Molecular functioni
- transcription coregulator activity Source: UniProtKB
GO - Biological processi
- regulation of transcription by RNA polymerase II Source: UniProtKB
- regulation of transcription initiation from RNA polymerase II promoter Source: FlyBase
Keywordsi
Molecular function | Activator |
Biological process | Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: Mediator of RNA polymerase II transcription subunit 14Alternative name(s): Mediator complex subunit 14 dRGR1 |
Gene namesi | Name:MED14 Synonyms:Trap170 ORF Names:CG12031 |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Holometabola › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0035145, MED14 |
Subcellular locationi
Nucleus
- Nucleus Curated
Nucleus
- core mediator complex Source: FlyBase
- mediator complex Source: UniProtKB
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000304588 | 1 – 1553 | Mediator of RNA polymerase II transcription subunit 14Add BLAST | 1553 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 615 | Phosphoserine1 Publication | 1 | |
Modified residuei | 1015 | Phosphoserine1 Publication | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | Q9W0P8 |
PTM databases
iPTMneti | Q9W0P8 |
Expressioni
Gene expression databases
Bgeei | FBgn0035145, Expressed in female gonad and 18 other tissues |
ExpressionAtlasi | Q9W0P8, baseline and differential |
Genevisiblei | Q9W0P8, DM |
Interactioni
Subunit structurei
Component of the Mediator complex, which may include CDK8, MED4, MED6, MED11, MED14, MED17, MED18, MED20, MED21, MED22, MED27, MED28, MED30 and MED31.
1 PublicationProtein-protein interaction databases
BioGRIDi | 63628, 49 interactors |
DIPi | DIP-60711N |
IntActi | Q9W0P8, 2 interactors |
STRINGi | 7227.FBpp0072453 |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 55 – 59 | LXXLL motif 1 | 5 | |
Motifi | 472 – 476 | LXXLL motif 2 | 5 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 1048 – 1172 | Pro-richAdd BLAST | 125 | |
Compositional biasi | 1496 – 1552 | Pro-richAdd BLAST | 57 |
Sequence similaritiesi
Belongs to the Mediator complex subunit 14 family.Curated
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG1875, Eukaryota |
GeneTreei | ENSGT00390000001021 |
HOGENOMi | CLU_001928_0_0_1 |
InParanoidi | Q9W0P8 |
OMAi | HLDCPIT |
PhylomeDBi | Q9W0P8 |
Family and domain databases
InterProi | View protein in InterPro IPR013947, Mediator_Med14 |
PANTHERi | PTHR12809, PTHR12809, 1 hit |
Pfami | View protein in Pfam PF08638, Med14, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketIsoform 1 (identifier: Q9W0P8-1) [UniParc]FASTAAdd to basket
Also known as: AImported
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAPTPLPLEQ MPGVGGGGGG YLPAAQEGGP RINTMSMSVL IDFIIQRTYH
60 70 80 90 100
ELTVLAELLP RKTDMERKVE IYNYAARTRH LFTRLNALVK WGNSVSKVDK
110 120 130 140 150
SSQIMSFLDK QNMLFVETAD MLARMSRETL VRARLPNFHI PAAVEVLTTG
160 170 180 190 200
TYNRLPTCIR ERIVPADAIT PAEKRQTLLR LNQVIQHRLV TGKLLPQMRE
210 220 230 240 250
FRIRNGRVTF EVKHEFSVSL TVMGDNPTVP WRLLDIDVLV EDKETGDGKS
260 270 280 290 300
LVHPLQVNYI HQLIQARLVE NPNALSEVYN CLHYFCQSLQ LEVLYTQTLR
310 320 330 340 350
LNYERLDDNN ITVEEYVPGV KLTVSYWRDL KSELGYRLTV QSDPSEIGRP
360 370 380 390 400
LAVVHVPSLG AKESAEVADR AVRSEHLSME RLIVHTVYIR SVSRLSDLKL
410 420 430 440 450
EFQAFLKDVD FNLQGTPAIL TVPVLSPCLR AEQIHITIDT HTGMFRCHVP
460 470 480 490 500
KHLDCPIMEE MQDCLNGDRS KLPALLSELR FWITHRRCDK TLQHLPATAT
510 520 530 540 550
ETLPFLVQPD QEILQPGRHK IYVKLHRHPN IVLVVQLKEK TTMPNEMEYT
560 570 580 590 600
FHLGFVAYQK DELDVIDDSA MQLVSIVAQP HSDIPKCYTK LMRLIEFDTF
610 620 630 640 650
VATHGPGTEV DAEVSPHKRK SNGDLLAPPA KQQKTIFPAY FIPELAHVVA
660 670 680 690 700
MCDEKIPFMN LAQTLSKHNI PHSGLQVEAN ATSLVLKILA LPQPGKTTFA
710 720 730 740 750
TQQQQQQGAP AVAGENKPSG TSGLPKIDSH VWDDLMRRVL SISIRSQTNK
760 770 780 790 800
NSQVRIWVVE FVFYSTPLQS SHPKEQGSRR TVYLTYEQAN YDFSKTVEDL
810 820 830 840 850
LNDWSKIVYL YTLVYDFAEQ LLNKRLSLCD MLVVKSYSYM NLLLGYGPKK
860 870 880 890 900
EVSCNIYWSV QSHGFRLTFV GGMSAVNGHS MMRDQLAQHL NQQHSITQIA
910 920 930 940 950
QILHETYNPL SSIAKLPVLP FLGIPRPQVP VLSFCVLAQS PCLIRLTYQA
960 970 980 990 1000
VYCLELRFRA NRLVSIRDGA SSRFERNVVE EFTPIQGLKA FLSKYVDESA
1010 1020 1030 1040 1050
VYRGRASHED DNPLSPMGME DNFGGPSSVA GVSAGGSSPF LGTGMRGPQS
1060 1070 1080 1090 1100
PRDSGLRFPA PHTPPSSSNP HTPASPHPSA GAGGGSGPQG HGNFNLTSPP
1110 1120 1130 1140 1150
APHMPHPSPS GLMPSSPLNP QPSPHMVHSP GPNTLYMQSH QDSPFTAMSP
1160 1170 1180 1190 1200
ANNNWPGSPS MPRPSPRPGQ SPDHKSTGGG AGVAGGTDRG GSRGTLNRPW
1210 1220 1230 1240 1250
AGAVPTLLTH EALETLCRPS PYPNKDINVP DMSPLERFLG CVYMRRQLHR
1260 1270 1280 1290 1300
NIQSEETLTA LNSTEPGVVL FKVDGLQCQV VLNQMHMQTL HLKVSQLPPM
1310 1320 1330 1340 1350
PDKHPPPFQL SQDDLLVIEQ YFDTRVAAPP YRPNSLHSIC RLLNLPAQVL
1360 1370 1380 1390 1400
KDFVQIMRLD LKPELGGDQL KWTVQICLRM PPSAVPIVPS GNACVVMGRM
1410 1420 1430 1440 1450
KILFFLQITR IPYGAVIGVG KDWKDSPSLV LPIVYDIQTN VTQLAERTGQ
1460 1470 1480 1490 1500
VISPTMTAAS TLLRRFAEFN AQQNQCTLFP AITDLLTNLQ LAAEMPQPPP
1510 1520 1530 1540 1550
NQSIGPPVGV GVGVGSSPNP MMPMQQLPQQ VGPQGPVGPG GYPQMGPNPG
GPQ
Sequence cautioni
The sequence AAG02462 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
The sequence AAK84939 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 698 | T → N in AAK84939 (PubMed:12537569).Curated | 1 | |
Sequence conflicti | 698 | T → N in AAV36836 (Ref. 3) Curated | 1 | |
Sequence conflicti | 822 | L → Q in AAK84939 (PubMed:12537569).Curated | 1 | |
Sequence conflicti | 822 | L → Q in AAV36836 (Ref. 3) Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_058119 | 410 | D → DCE in isoform 2. Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE014296 Genomic DNA Translation: AAF47395.4 AE014296 Genomic DNA Translation: AAN11445.2 BT015951 mRNA Translation: AAV36836.1 AF289997 mRNA Translation: AAG02462.1 Different initiation. AY050240 mRNA Translation: AAK84939.1 Different initiation. |
RefSeqi | NP_612041.2, NM_138197.3 [Q9W0P8-1] NP_728534.2, NM_167835.2 [Q9W0P8-3] |
Genome annotation databases
EnsemblMetazoai | FBtr0072554; FBpp0072453; FBgn0035145 [Q9W0P8-1] FBtr0344912; FBpp0311212; FBgn0035145 [Q9W0P8-3] |
GeneIDi | 38073 |
KEGGi | dme:Dmel_CG12031 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE014296 Genomic DNA Translation: AAF47395.4 AE014296 Genomic DNA Translation: AAN11445.2 BT015951 mRNA Translation: AAV36836.1 AF289997 mRNA Translation: AAG02462.1 Different initiation. AY050240 mRNA Translation: AAK84939.1 Different initiation. |
RefSeqi | NP_612041.2, NM_138197.3 [Q9W0P8-1] NP_728534.2, NM_167835.2 [Q9W0P8-3] |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 63628, 49 interactors |
DIPi | DIP-60711N |
IntActi | Q9W0P8, 2 interactors |
STRINGi | 7227.FBpp0072453 |
PTM databases
iPTMneti | Q9W0P8 |
Proteomic databases
PaxDbi | Q9W0P8 |
Genome annotation databases
EnsemblMetazoai | FBtr0072554; FBpp0072453; FBgn0035145 [Q9W0P8-1] FBtr0344912; FBpp0311212; FBgn0035145 [Q9W0P8-3] |
GeneIDi | 38073 |
KEGGi | dme:Dmel_CG12031 |
Organism-specific databases
CTDi | 9282 |
FlyBasei | FBgn0035145, MED14 |
Phylogenomic databases
eggNOGi | KOG1875, Eukaryota |
GeneTreei | ENSGT00390000001021 |
HOGENOMi | CLU_001928_0_0_1 |
InParanoidi | Q9W0P8 |
OMAi | HLDCPIT |
PhylomeDBi | Q9W0P8 |
Miscellaneous databases
BioGRID-ORCSi | 38073, 0 hits in 1 CRISPR screen |
GenomeRNAii | 38073 |
PROi | PR:Q9W0P8 |
Gene expression databases
Bgeei | FBgn0035145, Expressed in female gonad and 18 other tissues |
ExpressionAtlasi | Q9W0P8, baseline and differential |
Genevisiblei | Q9W0P8, DM |
Family and domain databases
InterProi | View protein in InterPro IPR013947, Mediator_Med14 |
PANTHERi | PTHR12809, PTHR12809, 1 hit |
Pfami | View protein in Pfam PF08638, Med14, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MED14_DROME | |
Accessioni | Q9W0P8Primary (citable) accession number: Q9W0P8 Secondary accession number(s): Q5U1E1 Q9GYW6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 11, 2007 |
Last sequence update: | June 1, 2003 | |
Last modified: | December 2, 2020 | |
This is version 122 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - SIMILARITY comments
Index of protein domains and families