UniProtKB - Q9W0G1 (PTP61_DROME)
Protein
Tyrosine-protein phosphatase non-receptor type 61F
Gene
Ptp61F
Organism
Drosophila melanogaster (Fruit fly)
Status
Functioni
Non-receptor protein tyrosine phosphatase required for maintaining Dock in its non-phosphorylated state.2 Publications
Catalytic activityi
- H2O + O-phospho-L-tyrosyl-[protein] = L-tyrosyl-[protein] + phosphatePROSITE-ProRule annotation1 PublicationEC:3.1.3.48PROSITE-ProRule annotation1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 203 | SubstrateBy similarity | 1 | |
Active sitei | 237 | Phosphocysteine intermediatePROSITE-ProRule annotationBy similarity | 1 | |
Binding sitei | 281 | SubstrateBy similarity | 1 |
GO - Molecular functioni
- protein tyrosine phosphatase activity Source: FlyBase
GO - Biological processi
- axon guidance Source: FlyBase
- cellular response to hypoxia Source: FlyBase
- germ-band extension Source: UniProtKB
- mitotic cell cycle Source: FlyBase
- negative regulation of cell population proliferation Source: FlyBase
- negative regulation of epidermal growth factor receptor signaling pathway Source: FlyBase
- negative regulation of ERK1 and ERK2 cascade Source: FlyBase
- negative regulation of insulin receptor signaling pathway Source: FlyBase
- negative regulation of JNK cascade Source: FlyBase
- negative regulation of phosphatidylinositol 3-kinase signaling Source: FlyBase
- negative regulation of receptor signaling pathway via JAK-STAT Source: FlyBase
- negative regulation of vascular endothelial growth factor receptor signaling pathway Source: FlyBase
- peptidyl-tyrosine dephosphorylation Source: FlyBase
- peptidyl-tyrosine phosphorylation Source: FlyBase
- receptor signaling pathway via JAK-STAT Source: Reactome
- regulation of protein localization Source: FlyBase
- regulation of protein stability Source: FlyBase
Keywordsi
Molecular function | Hydrolase, Protein phosphatase |
Enzyme and pathway databases
Reactomei | R-DME-210688, Dephosphorylation by PTP61F phosphatases [Q9W0G1-1] R-DME-210693, STAT92E dimer dephosphorylated in the nucleus and transported to the cytosol [Q9W0G1-2] R-DME-877312, Regulation of IFNG signaling [Q9W0G1-2] R-DME-8849472, PTK6 Down-Regulation [Q9W0G1-2] |
Names & Taxonomyi
Protein namesi | Recommended name: Tyrosine-protein phosphatase non-receptor type 61F (EC:3.1.3.48)Alternative name(s): dPTP61F |
Gene namesi | Name:Ptp61F ORF Names:CG9181 |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Holometabola › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0267487, Ptp61F |
Subcellular locationi
Cytoplasm and Cytosol
Other locations
Note: Associates with the membranes of the reticular network and the mitochondria.
Nucleus
Cytosol
- cytosol Source: Reactome
Mitochondrion
- mitochondrial envelope Source: UniProtKB
Nucleus
- nucleus Source: FlyBase
Other locations
- cytoplasm Source: FlyBase
- endomembrane system Source: UniProtKB-SubCell
- membrane Source: UniProtKB-KW
- perinuclear region of cytoplasm Source: FlyBase
Keywords - Cellular componenti
Cytoplasm, Membrane, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000094853 | 1 – 548 | Tyrosine-protein phosphatase non-receptor type 61FAdd BLAST | 548 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 83 | Phosphoserine1 Publication | 1 | |
Modified residuei | 86 | Phosphotyrosine1 Publication | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | Q9W0G1 |
PRIDEi | Q9W0G1 |
PTM databases
iPTMneti | Q9W0G1 |
Expressioni
Tissue specificityi
Expressed during oogenesis and embryogenesis. Isoform A and isoform B are expressed in distinct patterns. Isoform B is expressed in the mesoderm and neuroblast layer during germband extension and later in the gut epithelia. Isoform A accumulates in 16 segmentally repeated stripes in the ectoderm during germband extension. These stripes are flanked by, and adjacent to, the domains of engrailed and wingless gene expression in the anterior/posterior axis. In stage 10 embryos, isoform A colocalizes with the area lateral to the denticle belts that will give rise to naked cuticle. Isoform A is also expressed later in embryogenesis in the central nervous system.1 Publication
Gene expression databases
Bgeei | FBgn0267487, Expressed in egg cell and 46 other tissues |
ExpressionAtlasi | Q9W0G1, baseline and differential |
Genevisiblei | Q9W0G1, DM |
Interactioni
Binary interactionsi
Q9W0G1
With | #Exp. | IntAct |
---|---|---|
dock [Q24218] | 5 | EBI-74714,EBI-74727 |
Protein-protein interaction databases
BioGRIDi | 63702, 20 interactors |
IntActi | Q9W0G1, 5 interactors |
STRINGi | 7227.FBpp0072602 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 33 – 296 | Tyrosine-protein phosphatasePROSITE-ProRule annotationAdd BLAST | 264 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 237 – 243 | Substrate bindingBy similarity | 7 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0789, Eukaryota |
GeneTreei | ENSGT00940000154686 |
InParanoidi | Q9W0G1 |
OMAi | CETYQNF |
PhylomeDBi | Q9W0G1 |
Family and domain databases
Gene3Di | 3.90.190.10, 1 hit |
InterProi | View protein in InterPro IPR029021, Prot-tyrosine_phosphatase-like IPR000242, PTPase_domain IPR016130, Tyr_Pase_AS IPR003595, Tyr_Pase_cat IPR000387, TYR_PHOSPHATASE_dom |
Pfami | View protein in Pfam PF00102, Y_phosphatase, 1 hit |
PRINTSi | PR00700, PRTYPHPHTASE |
SMARTi | View protein in SMART SM00194, PTPc, 1 hit SM00404, PTPc_motif, 1 hit |
SUPFAMi | SSF52799, SSF52799, 1 hit |
PROSITEi | View protein in PROSITE PS00383, TYR_PHOSPHATASE_1, 1 hit PS50056, TYR_PHOSPHATASE_2, 1 hit PS50055, TYR_PHOSPHATASE_PTP, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform A1 Publication (identifier: Q9W0G1-1) [UniParc]FASTAAdd to basket
Also known as: Cytoplasmic1 Publication, DPTP61Fm
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSEQKTSGSG SAAAARLQIE AEYKDKGPQW HRFYKEICET CDREAKEKQF
60 70 80 90 100
STSESERHTN RGLNRYRDVN PYDHSRIVLK RGSVDYINAN LVQLERAERQ
110 120 130 140 150
YILTQGPLVD TVGHFWLMVW EQKSRAVLML NKLMEKKQIK CHLYWPNEMG
160 170 180 190 200
ADKALKLPHV KLTVELVRLE TYQNFVRRWF KLTDLETQQS REVMQFHYTT
210 220 230 240 250
WPDFGIPSSP NAFLKFLQQV RDSGCLSRDV GPAVVHCSAG IGRSGTFCLV
260 270 280 290 300
DCCLVLIDKY GECNVSKVLC ELRSYRMGLI QTADQLDFSY QAIIEGIKKL
310 320 330 340 350
HDPTFLDAEE PLISNDTETH TLDELPPPLP PRVQSLNLPL APNSGGILSL
360 370 380 390 400
NMRAAQANGA ESIGKELSKD ALNNFINQHD MIHDAEVADS RPLPPLPVRA
410 420 430 440 450
FNDSDSDEDY LLDDDDEDDT DEDEEYETIN EHDADPVNGH VPATTQPHAD
460 470 480 490 500
DVNANNEKPA VPVDEQHKAN GIDPIPGQLP ASPENELKRR KRNEYQASLE
510 520 530 540
QKVNDMKRKQ RENEDKQLAA KKRRSLLTYI AAGVVVGVIC AYAYTKLG
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketQ8IRH4 | Q8IRH4_DROME | Protein tyrosine phosphatase 61F, i... | Ptp61F anon-WO0118547.297, anon-WO0140519.98, BEST:LP01280, CG9178, CPtp62A | 431 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 274 | S → T (PubMed:8463208).Curated | 1 | |
Sequence conflicti | 274 | S → T (Ref. 2) Curated | 1 | |
Sequence conflicti | 382 | I → F in AAA75348 (PubMed:8463208).Curated | 1 | |
Sequence conflicti | 382 | I → F in AAA75349 (PubMed:8463208).Curated | 1 | |
Sequence conflicti | 382 | I → F in AAA75338 (PubMed:8463208).Curated | 1 | |
Sequence conflicti | 382 | I → F in AAA75339 (PubMed:8463208).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_050733 | 1 – 34 | MSEQK…WHRFY → MTKFAQRSKKTCEIRGTMDV CVCVCVSMW in isoform C. 2 PublicationsAdd BLAST | 34 | |
Alternative sequenceiVSP_050259 | 525 – 548 | SLLTY…YTKLG → RGNRLKKSKTK in isoform B and isoform C. 2 PublicationsAdd BLAST | 24 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L11250, L11249 Genomic DNA Translation: AAA75348.1 L11251, L11249, L11250 Genomic DNA Translation: AAA75349.1 L11252 mRNA Translation: AAA75338.1 L11253 mRNA Translation: AAA75339.1 L14849 mRNA Translation: AAA28748.1 AE014296 Genomic DNA Translation: AAF47486.1 AE014296 Genomic DNA Translation: AAF47487.1 AE014296 Genomic DNA Translation: AAN11475.1 AY069713 mRNA Translation: AAL39858.1 |
PIRi | A46101 B46101 |
RefSeqi | NP_476687.1, NM_057339.5 [Q9W0G1-1] NP_476688.1, NM_057340.4 [Q9W0G1-2] NP_728600.1, NM_167874.2 [Q9W0G1-3] |
Genome annotation databases
EnsemblMetazoai | FBtr0072716; FBpp0072600; FBgn0267487 [Q9W0G1-3] FBtr0072717; FBpp0072601; FBgn0267487 [Q9W0G1-2] FBtr0072718; FBpp0072602; FBgn0267487 [Q9W0G1-1] |
GeneIDi | 38160 |
KEGGi | dme:Dmel_CG9181 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L11250, L11249 Genomic DNA Translation: AAA75348.1 L11251, L11249, L11250 Genomic DNA Translation: AAA75349.1 L11252 mRNA Translation: AAA75338.1 L11253 mRNA Translation: AAA75339.1 L14849 mRNA Translation: AAA28748.1 AE014296 Genomic DNA Translation: AAF47486.1 AE014296 Genomic DNA Translation: AAF47487.1 AE014296 Genomic DNA Translation: AAN11475.1 AY069713 mRNA Translation: AAL39858.1 |
PIRi | A46101 B46101 |
RefSeqi | NP_476687.1, NM_057339.5 [Q9W0G1-1] NP_476688.1, NM_057340.4 [Q9W0G1-2] NP_728600.1, NM_167874.2 [Q9W0G1-3] |
3D structure databases
SMRi | Q9W0G1 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 63702, 20 interactors |
IntActi | Q9W0G1, 5 interactors |
STRINGi | 7227.FBpp0072602 |
PTM databases
iPTMneti | Q9W0G1 |
Proteomic databases
PaxDbi | Q9W0G1 |
PRIDEi | Q9W0G1 |
Genome annotation databases
EnsemblMetazoai | FBtr0072716; FBpp0072600; FBgn0267487 [Q9W0G1-3] FBtr0072717; FBpp0072601; FBgn0267487 [Q9W0G1-2] FBtr0072718; FBpp0072602; FBgn0267487 [Q9W0G1-1] |
GeneIDi | 38160 |
KEGGi | dme:Dmel_CG9181 |
Organism-specific databases
CTDi | 38160 |
FlyBasei | FBgn0267487, Ptp61F |
Phylogenomic databases
eggNOGi | KOG0789, Eukaryota |
GeneTreei | ENSGT00940000154686 |
InParanoidi | Q9W0G1 |
OMAi | CETYQNF |
PhylomeDBi | Q9W0G1 |
Enzyme and pathway databases
Reactomei | R-DME-210688, Dephosphorylation by PTP61F phosphatases [Q9W0G1-1] R-DME-210693, STAT92E dimer dephosphorylated in the nucleus and transported to the cytosol [Q9W0G1-2] R-DME-877312, Regulation of IFNG signaling [Q9W0G1-2] R-DME-8849472, PTK6 Down-Regulation [Q9W0G1-2] |
Miscellaneous databases
BioGRID-ORCSi | 38160, 0 hits in 3 CRISPR screens |
ChiTaRSi | Ptp61F, fly |
GenomeRNAii | 38160 |
PROi | PR:Q9W0G1 |
Gene expression databases
Bgeei | FBgn0267487, Expressed in egg cell and 46 other tissues |
ExpressionAtlasi | Q9W0G1, baseline and differential |
Genevisiblei | Q9W0G1, DM |
Family and domain databases
Gene3Di | 3.90.190.10, 1 hit |
InterProi | View protein in InterPro IPR029021, Prot-tyrosine_phosphatase-like IPR000242, PTPase_domain IPR016130, Tyr_Pase_AS IPR003595, Tyr_Pase_cat IPR000387, TYR_PHOSPHATASE_dom |
Pfami | View protein in Pfam PF00102, Y_phosphatase, 1 hit |
PRINTSi | PR00700, PRTYPHPHTASE |
SMARTi | View protein in SMART SM00194, PTPc, 1 hit SM00404, PTPc_motif, 1 hit |
SUPFAMi | SSF52799, SSF52799, 1 hit |
PROSITEi | View protein in PROSITE PS00383, TYR_PHOSPHATASE_1, 1 hit PS50056, TYR_PHOSPHATASE_2, 1 hit PS50055, TYR_PHOSPHATASE_PTP, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PTP61_DROME | |
Accessioni | Q9W0G1Primary (citable) accession number: Q9W0G1 Secondary accession number(s): Q27932, Q8SZY3, Q9W0G2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 10, 2004 |
Last sequence update: | May 1, 2000 | |
Last modified: | December 2, 2020 | |
This is version 168 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - SIMILARITY comments
Index of protein domains and families