UniProtKB - Q9VZZ4 (PXDN_DROME)
Protein
Peroxidasin
Gene
Pxn
Organism
Drosophila melanogaster (Fruit fly)
Status
Functioni
Plays a role in extracellular matrix consolidation, phagocytosis and defense.1 Publication
Catalytic activityi
- EC:1.11.1.7
Cofactori
Protein has several cofactor binding sites:- Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity
- heme bBy similarityNote: Binds 1 heme b (iron(II)-protoporphyrin IX) group covalently per subunit.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 862 | Heme (covalent; via 2 links)By similarity | 1 | |
Active sitei | 863 | Proton acceptorPROSITE-ProRule annotation | 1 | |
Metal bindingi | 864 | CalciumPROSITE-ProRule annotation | 1 | |
Metal bindingi | 941 | CalciumPROSITE-ProRule annotation | 1 | |
Metal bindingi | 943 | Calcium; via carbonyl oxygenPROSITE-ProRule annotation | 1 | |
Metal bindingi | 945 | CalciumPROSITE-ProRule annotation | 1 | |
Metal bindingi | 947 | CalciumPROSITE-ProRule annotation | 1 | |
Sitei | 1012 | Transition state stabilizerPROSITE-ProRule annotation | 1 | |
Binding sitei | 1015 | Heme (covalent; via 2 links)By similarity | 1 | |
Metal bindingi | 1109 | Iron (heme axial ligand)PROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- bromide peroxidase activity Source: FlyBase
- heme binding Source: InterPro
- metal ion binding Source: UniProtKB-KW
- peroxidase activity Source: GO_Central
GO - Biological processi
- basement membrane assembly Source: FlyBase
- collagen fibril organization Source: FlyBase
- extracellular matrix organization Source: UniProtKB
- hydrogen peroxide catabolic process Source: UniProtKB-KW
- phagocytosis Source: UniProtKB
- response to oxidative stress Source: InterPro
Keywordsi
Molecular function | Oxidoreductase, Peroxidase |
Biological process | Hydrogen peroxide |
Ligand | Calcium, Heme, Iron, Metal-binding |
Enzyme and pathway databases
Reactomei | R-DME-209968, Thyroxine biosynthesis R-DME-6798695, Neutrophil degranulation R-DME-8941413, Events associated with phagocytolytic activity of PMN cells |
Protein family/group databases
PeroxiBasei | 3369, DmPxd-A 3370, DmPxd 7646, CflPxd01 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Pxn ORF Names:CG12002 |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Holometabola › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0011828, Pxn |
Subcellular locationi
Extracellular region or secreted
- Secreted Curated
Extracellular region or secreted
- collagen-containing extracellular matrix Source: FlyBase
- extracellular matrix Source: FlyBase
- extracellular space Source: GO_Central
Keywords - Cellular componenti
SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 23 | Sequence analysisAdd BLAST | 23 | |
ChainiPRO_0000319624 | 24 – 1527 | PeroxidasinAdd BLAST | 1504 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 257 ↔ 307 | By similarity | ||
Disulfide bondi | 388 ↔ 437 | By similarity | ||
Glycosylationi | 419 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 479 ↔ 529 | By similarity | ||
Disulfide bondi | 574 ↔ 627 | By similarity | ||
Glycosylationi | 616 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 673 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 682 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 731 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 767 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 768 ↔ 784 | By similarity | ||
Disulfide bondi | 882 ↔ 892 | By similarity | ||
Disulfide bondi | 886 ↔ 909 | By similarity | ||
Glycosylationi | 962 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Disulfide bondi | 994 ↔ 1005 | By similarity | ||
Glycosylationi | 1120 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 1212 ↔ 1269 | By similarity | ||
Glycosylationi | 1213 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 1310 ↔ 1336 | By similarity |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
PaxDbi | Q9VZZ4 |
PRIDEi | Q9VZZ4 |
PTM databases
iPTMneti | Q9VZZ4 |
Expressioni
Tissue specificityi
Expressed in hemocytes. Also expressed in the fat body and gastric caeca.1 Publication
Developmental stagei
Expressed throughout embryonic and larval development. Expressed in hemocytes as they migrate in the early embryo and later in embryogenesis, become localized to basement membranes.1 Publication
Gene expression databases
Bgeei | FBgn0011828, Expressed in embryonic/larval hemocyte (Drosophila) and 42 other tissues |
ExpressionAtlasi | Q9VZZ4, baseline and differential |
Genevisiblei | Q9VZZ4, DM |
Interactioni
Subunit structurei
Homotrimer; disulfide-linked.
1 PublicationProtein-protein interaction databases
BioGRIDi | 63831, 6 interactors |
IntActi | Q9VZZ4, 3 interactors |
STRINGi | 7227.FBpp0072828 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 24 – 52 | LRRNTAdd BLAST | 29 | |
Repeati | 53 – 74 | LRR 1Add BLAST | 22 | |
Repeati | 77 – 98 | LRR 2Add BLAST | 22 | |
Repeati | 101 – 122 | LRR 3Add BLAST | 22 | |
Repeati | 125 – 146 | LRR 4Add BLAST | 22 | |
Repeati | 149 – 170 | LRR 5Add BLAST | 22 | |
Domaini | 182 – 235 | LRRCTAdd BLAST | 54 | |
Domaini | 236 – 322 | Ig-like C2-type 1Add BLAST | 87 | |
Domaini | 365 – 453 | Ig-like C2-type 2Add BLAST | 89 | |
Domaini | 458 – 545 | Ig-like C2-type 3Add BLAST | 88 | |
Domaini | 553 – 643 | Ig-like C2-type 4Add BLAST | 91 | |
Domaini | 1463 – 1524 | VWFCPROSITE-ProRule annotationAdd BLAST | 62 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 1403 – 1441 | Sequence analysisAdd BLAST | 39 |
Sequence similaritiesi
Keywords - Domaini
Coiled coil, Immunoglobulin domain, Leucine-rich repeat, Repeat, SignalPhylogenomic databases
eggNOGi | KOG0619, Eukaryota KOG2408, Eukaryota |
GeneTreei | ENSGT00940000168557 |
HOGENOMi | CLU_006087_0_1_1 |
InParanoidi | Q9VZZ4 |
OMAi | NCVPRIT |
PhylomeDBi | Q9VZZ4 |
Family and domain databases
CDDi | cd09826, peroxidasin_like, 1 hit |
Gene3Di | 1.10.640.10, 1 hit 2.60.40.10, 4 hits 3.80.10.10, 1 hit |
InterProi | View protein in InterPro IPR019791, Haem_peroxidase_animal IPR010255, Haem_peroxidase_sf IPR037120, Haem_peroxidase_sf_animal IPR007110, Ig-like_dom IPR036179, Ig-like_dom_sf IPR013783, Ig-like_fold IPR013098, Ig_I-set IPR003599, Ig_sub IPR003598, Ig_sub2 IPR001611, Leu-rich_rpt IPR003591, Leu-rich_rpt_typical-subtyp IPR032675, LRR_dom_sf IPR000372, LRRNT IPR034824, Peroxidasin_peroxidase IPR001007, VWF_dom |
Pfami | View protein in Pfam PF03098, An_peroxidase, 1 hit PF07679, I-set, 3 hits PF13855, LRR_8, 2 hits PF00093, VWC, 1 hit |
PRINTSi | PR00457, ANPEROXIDASE |
SMARTi | View protein in SMART SM00409, IG, 4 hits SM00408, IGc2, 4 hits SM00369, LRR_TYP, 5 hits SM00013, LRRNT, 1 hit SM00214, VWC, 1 hit |
SUPFAMi | SSF48113, SSF48113, 1 hit SSF48726, SSF48726, 4 hits |
PROSITEi | View protein in PROSITE PS50835, IG_LIKE, 4 hits PS50292, PEROXIDASE_3, 1 hit PS01208, VWFC_1, 1 hit PS50184, VWFC_2, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketIsoform A (identifier: Q9VZZ4-1) [UniParc]FASTAAdd to basket
Also known as: C, D, E
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MRFMLLMLQL LGLLLLLAGG VQSVYCPAGC TCLERTVRCI RAKLSAVPKL
60 70 80 90 100
PQDTQTLDLR FNHIEELPAN AFSGLAQLTT LFLNDNELAY LQDGALNGLT
110 120 130 140 150
ALRFVYLNNN RLSRLPATIF QRMPRLEAIF LENNDIWQLP AGLFDNLPRL
160 170 180 190 200
NRLIMYNNKL TQLPVDGFNR LNNLKRLRLD GNAIDCNCGV YSLWRRWHLD
210 220 230 240 250
VQRQLVSISL TCAAPQMLQN QGFSSLGEHH FKCAKPQFLV APQDAQVAAG
260 270 280 290 300
EQVELSCEVT GLPRPQITWM HNTQELGLEE QAQAEILPSG SLLIRSADTS
310 320 330 340 350
DMGIYQCIAR NEMGALRSQP VRLVVNGGNH PLDSPIDARS NQVWADAGTP
360 370 380 390 400
MHGATPLPSP LPSPPHFTHQ PHDQIVALHG SGHVLLDCAA SGWPQPDIQW
410 420 430 440 450
FVNGRQLLQS TPSLQLQANG SLILLQPNQL SAGTYRCEAR NSLGSVQATA
460 470 480 490 500
RIELKELPEI LTAPQSQTIK LGKAFVLECD ADGNPLPTID WQLNGVPLPG
510 520 530 540 550
NTPDLQLENE NTELVVGAAR QEHAGVYRCT AHNENGETSV EATIKVERSQ
560 570 580 590 600
SPPQLAIEPS NLVAITGTTI ELPCQADQPE DGLQISWRHD GRLIDPNVQL
610 620 630 640 650
AEKYQISGAG SLFVKNVTIP DGGRYECQLK NQFGRASASA LVTIRNNVDL
660 670 680 690 700
APGDRYVRIA FAEAAKEIDL AINNTLDMLF SNRSDKAPPN YGELLRVFRF
710 720 730 740 750
PTGEARQLAR AAEIYERTLV NIRKHVQEGD NLTMKSEEYE FRDLLSREHL
760 770 780 790 800
HLVAELSGCM EHREMPNCTD MCFHSRYRSI DGTCNNLQHP TWGASLTAFR
810 820 830 840 850
RLAPPIYENG FSMPVGWTKG MLYSGHAKPS ARLVSTSLVA TKEITPDARI
860 870 880 890 900
THMVMQWGQF LDHDLDHAIP SVSSESWDGI DCKKSCEMAP PCYPIEVPPN
910 920 930 940 950
DPRVRNRRCI DVVRSSAICG SGMTSLFFDS VQHREQINQL TSYIDASQVY
960 970 980 990 1000
GYSTAFAQEL RNLTSQEGLL RVGVHFPRQK DMLPFAAPQD GMDCRRNLDE
1010 1020 1030 1040 1050
NTMSCFVSGD IRVNEQVGLL AMHTIWMREH NRIASKLKQI NSHWDGDTLY
1060 1070 1080 1090 1100
QEARKIVGAQ MQHITFKQWL PLIIGESGME MMGEYQGYNP QLNPSIANEF
1110 1120 1130 1140 1150
ATAALRFGHT IINPILHRLN ETFQPIPQGH LLLHKAFFAP WRLAYEGGVD
1160 1170 1180 1190 1200
PLMRGFLAVP AKLKTPDQNL NTELTEKLFQ TAHAVALDLA AINIQRGRDH
1210 1220 1230 1240 1250
GMPGYNVYRK LCNLTVAQDF EDLAGEISSA EIRQKMKELY GHPDNVDVWL
1260 1270 1280 1290 1300
GGILEDQVEG GKVGPLFQCL LVEQFRRLRD GDRLYYENPG VFSPEQLTQI
1310 1320 1330 1340 1350
KQANFGRVLC DVGDNFDQVT ENVFILAKHQ GGYKKCEDII GINLYLWQEC
1360 1370 1380 1390 1400
GRCNSPPAIF DSYIPQTYTK RSNRQKRDLG KENDEVATAE SYDSPLESLY
1410 1420 1430 1440 1450
DVNEERVSGL EELIGSFQKE LKKLHKKLRK LEDSCNSADS EPVAQVVQLA
1460 1470 1480 1490 1500
AAPPQLVSKP KRSHCVDDKG TTRLNNEVWS PDVCTKCNCF HGQVNCLRER
1510 1520
CGEVSCPPGV DPLTPPEACC PHCPMVK
Sequence cautioni
The sequence AAA61568 differs from that shown. Reason: Frameshift.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 128 | A → G in AAA61568 (PubMed:8062820).Curated | 1 | |
Sequence conflicti | 183 | A → AID in AAA61568 (PubMed:8062820).Curated | 1 | |
Sequence conflicti | 263 | P → H in AAA61568 (PubMed:8062820).Curated | 1 | |
Sequence conflicti | 282 | A → T in AAA61568 (PubMed:8062820).Curated | 1 | |
Sequence conflicti | 294 | I → H in AAA61568 (PubMed:8062820).Curated | 1 | |
Sequence conflicti | 351 | M → T in AAA61568 (PubMed:8062820).Curated | 1 | |
Sequence conflicti | 361 – 362 | LP → SPSH in AAA61568 (PubMed:8062820).Curated | 2 | |
Sequence conflicti | 380 | G → S in AAA61568 (PubMed:8062820).Curated | 1 | |
Sequence conflicti | 692 | G → D in AAA61568 (PubMed:8062820).Curated | 1 | |
Sequence conflicti | 959 – 960 | EL → LA in AAA61568 (PubMed:8062820).Curated | 2 | |
Sequence conflicti | 1083 | G → S in AAA61568 (PubMed:8062820).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_032693 | 456 – 457 | EL → GE in isoform B. 1 Publication | 2 | |
Alternative sequenceiVSP_032694 | 458 – 1527 | Missing in isoform B. 1 PublicationAdd BLAST | 1070 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U11052 mRNA Translation: AAA61568.1 Frameshift. AE014296 Genomic DNA Translation: AAF47668.1 AE014296 Genomic DNA Translation: AAN11518.1 AE014296 Genomic DNA Translation: AAS64946.1 AE014296 Genomic DNA Translation: AAS64947.1 AE014296 Genomic DNA Translation: AAS64948.1 AY051536 mRNA Translation: AAK92960.1 AY052120 mRNA Translation: AAK93544.1 |
RefSeqi | NP_523891.2, NM_079167.5 [Q9VZZ4-1] NP_728759.1, NM_167957.3 [Q9VZZ4-2] NP_995975.1, NM_206253.3 [Q9VZZ4-1] NP_995976.1, NM_206254.3 [Q9VZZ4-1] NP_995977.1, NM_206255.3 [Q9VZZ4-1] |
Genome annotation databases
EnsemblMetazoai | FBtr0072950; FBpp0072827; FBgn0011828 [Q9VZZ4-2] FBtr0072951; FBpp0072828; FBgn0011828 [Q9VZZ4-1] FBtr0072952; FBpp0089205; FBgn0011828 [Q9VZZ4-1] FBtr0072953; FBpp0089215; FBgn0011828 [Q9VZZ4-1] FBtr0072954; FBpp0089216; FBgn0011828 [Q9VZZ4-1] |
GeneIDi | 38326 |
KEGGi | dme:Dmel_CG12002 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U11052 mRNA Translation: AAA61568.1 Frameshift. AE014296 Genomic DNA Translation: AAF47668.1 AE014296 Genomic DNA Translation: AAN11518.1 AE014296 Genomic DNA Translation: AAS64946.1 AE014296 Genomic DNA Translation: AAS64947.1 AE014296 Genomic DNA Translation: AAS64948.1 AY051536 mRNA Translation: AAK92960.1 AY052120 mRNA Translation: AAK93544.1 |
RefSeqi | NP_523891.2, NM_079167.5 [Q9VZZ4-1] NP_728759.1, NM_167957.3 [Q9VZZ4-2] NP_995975.1, NM_206253.3 [Q9VZZ4-1] NP_995976.1, NM_206254.3 [Q9VZZ4-1] NP_995977.1, NM_206255.3 [Q9VZZ4-1] |
3D structure databases
SMRi | Q9VZZ4 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 63831, 6 interactors |
IntActi | Q9VZZ4, 3 interactors |
STRINGi | 7227.FBpp0072828 |
Protein family/group databases
PeroxiBasei | 3369, DmPxd-A 3370, DmPxd 7646, CflPxd01 |
PTM databases
iPTMneti | Q9VZZ4 |
Proteomic databases
PaxDbi | Q9VZZ4 |
PRIDEi | Q9VZZ4 |
Genome annotation databases
EnsemblMetazoai | FBtr0072950; FBpp0072827; FBgn0011828 [Q9VZZ4-2] FBtr0072951; FBpp0072828; FBgn0011828 [Q9VZZ4-1] FBtr0072952; FBpp0089205; FBgn0011828 [Q9VZZ4-1] FBtr0072953; FBpp0089215; FBgn0011828 [Q9VZZ4-1] FBtr0072954; FBpp0089216; FBgn0011828 [Q9VZZ4-1] |
GeneIDi | 38326 |
KEGGi | dme:Dmel_CG12002 |
Organism-specific databases
CTDi | 5829 |
FlyBasei | FBgn0011828, Pxn |
Phylogenomic databases
eggNOGi | KOG0619, Eukaryota KOG2408, Eukaryota |
GeneTreei | ENSGT00940000168557 |
HOGENOMi | CLU_006087_0_1_1 |
InParanoidi | Q9VZZ4 |
OMAi | NCVPRIT |
PhylomeDBi | Q9VZZ4 |
Enzyme and pathway databases
Reactomei | R-DME-209968, Thyroxine biosynthesis R-DME-6798695, Neutrophil degranulation R-DME-8941413, Events associated with phagocytolytic activity of PMN cells |
Miscellaneous databases
BioGRID-ORCSi | 38326, 0 hits in 3 CRISPR screens |
ChiTaRSi | Pxn, fly |
GenomeRNAii | 38326 |
PROi | PR:Q9VZZ4 |
Gene expression databases
Bgeei | FBgn0011828, Expressed in embryonic/larval hemocyte (Drosophila) and 42 other tissues |
ExpressionAtlasi | Q9VZZ4, baseline and differential |
Genevisiblei | Q9VZZ4, DM |
Family and domain databases
CDDi | cd09826, peroxidasin_like, 1 hit |
Gene3Di | 1.10.640.10, 1 hit 2.60.40.10, 4 hits 3.80.10.10, 1 hit |
InterProi | View protein in InterPro IPR019791, Haem_peroxidase_animal IPR010255, Haem_peroxidase_sf IPR037120, Haem_peroxidase_sf_animal IPR007110, Ig-like_dom IPR036179, Ig-like_dom_sf IPR013783, Ig-like_fold IPR013098, Ig_I-set IPR003599, Ig_sub IPR003598, Ig_sub2 IPR001611, Leu-rich_rpt IPR003591, Leu-rich_rpt_typical-subtyp IPR032675, LRR_dom_sf IPR000372, LRRNT IPR034824, Peroxidasin_peroxidase IPR001007, VWF_dom |
Pfami | View protein in Pfam PF03098, An_peroxidase, 1 hit PF07679, I-set, 3 hits PF13855, LRR_8, 2 hits PF00093, VWC, 1 hit |
PRINTSi | PR00457, ANPEROXIDASE |
SMARTi | View protein in SMART SM00409, IG, 4 hits SM00408, IGc2, 4 hits SM00369, LRR_TYP, 5 hits SM00013, LRRNT, 1 hit SM00214, VWC, 1 hit |
SUPFAMi | SSF48113, SSF48113, 1 hit SSF48726, SSF48726, 4 hits |
PROSITEi | View protein in PROSITE PS50835, IG_LIKE, 4 hits PS50292, PEROXIDASE_3, 1 hit PS01208, VWFC_1, 1 hit PS50184, VWFC_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PXDN_DROME | |
Accessioni | Q9VZZ4Primary (citable) accession number: Q9VZZ4 Secondary accession number(s): Q23991, Q960D1, Q961K8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 26, 2008 |
Last sequence update: | May 1, 2000 | |
Last modified: | December 2, 2020 | |
This is version 166 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - SIMILARITY comments
Index of protein domains and families