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Protein

Protein-cysteine N-palmitoyltransferase Rasp

Gene

rasp

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Required in hedgehog (hh) expressing cells for production of appropriate signaling activity in embryos and in the imaginal precursors of adult tissues. Acts within the secretory pathway to catalyze N-terminal palmitoylation of Hh; this lipid modification is required for the embryonic and larval patterning activities of the Hh signal. Not required for Wg signaling.4 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei381Sequence analysis1

GO - Molecular functioni

  • palmitoyltransferase activity Source: FlyBase
  • transferase activity, transferring acyl groups Source: UniProtKB

GO - Biological processi

  • dorsal appendage formation Source: FlyBase
  • N-terminal peptidyl-L-cysteine N-palmitoylation Source: UniProtKB
  • protein palmitoylation Source: FlyBase
  • segment polarity determination Source: UniProtKB
  • smoothened signaling pathway Source: FlyBase

Keywordsi

Molecular functionAcyltransferase, Developmental protein, Segmentation polarity protein, Transferase

Enzyme and pathway databases

ReactomeiR-DME-209471 Formation and transport of the N-HH ligand
R-DME-5358346 Hedgehog ligand biogenesis
SignaLinkiQ9VZU2

Names & Taxonomyi

Protein namesi
Recommended name:
Protein-cysteine N-palmitoyltransferase Rasp (EC:2.3.1.-)
Alternative name(s):
Protein central missing
Protein sightless
Protein skinny hedgehog
Gene namesi
Name:rasp
Synonyms:cmn, sit, ski
ORF Names:CG11495
OrganismiDrosophila melanogaster (Fruit fly)Imported
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0024194 rasp

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei15 – 35HelicalSequence analysisAdd BLAST21
Transmembranei73 – 93HelicalSequence analysisAdd BLAST21
Transmembranei105 – 125HelicalSequence analysisAdd BLAST21
Transmembranei134 – 154HelicalSequence analysisAdd BLAST21
Transmembranei206 – 226HelicalSequence analysisAdd BLAST21
Transmembranei243 – 263HelicalSequence analysisAdd BLAST21
Transmembranei293 – 313HelicalSequence analysisAdd BLAST21
Transmembranei372 – 392HelicalSequence analysisAdd BLAST21
Transmembranei429 – 449HelicalSequence analysisAdd BLAST21
Transmembranei461 – 481HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002131361 – 500Protein-cysteine N-palmitoyltransferase RaspAdd BLAST500

Proteomic databases

PaxDbiQ9VZU2
PRIDEiQ9VZU2

Expressioni

Developmental stagei

Expressed both maternally and zygotically.1 Publication

Gene expression databases

BgeeiFBgn0024194
ExpressionAtlasiQ9VZU2 baseline and differential
GenevisibleiQ9VZU2 DM

Interactioni

Protein-protein interaction databases

BioGridi68830, 6 interactors
IntActiQ9VZU2, 3 interactors
STRINGi7227.FBpp0072893

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3860 Eukaryota
COG1696 LUCA
GeneTreeiENSGT00530000063629
InParanoidiQ9VZU2
OMAiIHYMSRD
OrthoDBiEOG091G0AFA
PhylomeDBiQ9VZU2

Family and domain databases

InterProiView protein in InterPro
IPR004299 MBOAT_fam
PfamiView protein in Pfam
PF03062 MBOAT, 1 hit

Sequencei

Sequence statusi: Complete.

Q9VZU2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRLPDRSLL TRCEIFVYFG VYIAYIVVGL YKIYGLRDHI VKEAKFQFPE
60 70 80 90 100
GWSLYPFSQR RRDDSNDELE NFGDFIVSFW PFYLLHVAVQ GFIRWKRPRL
110 120 130 140 150
QCLGFIGVCA LALSVNLDWS SMVLLVTLIA SYYIVSLLSL KFLVWLLSAG
160 170 180 190 200
WILCINVMQK NVWWTDRVGY TEYVLVIVTM SWSVLRGCSY SLSKIGAKQE
210 220 230 240 250
DLTRYSLVQY LGYAMYFPCL TYGPIISYQR FAARREDEVQ NWLGFVGGVL
260 270 280 290 300
RSAIWWLVMQ CALHYFYIHY MSRDVRMVEM MDSVFWQHSA GYFMGQFFFL
310 320 330 340 350
YYVVTYGLGI AFAVQDGIPA PNRPRCIGRI HFYSDMWKYF DEGLYEFLFQ
360 370 380 390 400
NIYAELCGKR SSAAAKFGAT ALTFAFVFVW HGCYTYVLIW SILNFLCLAA
410 420 430 440 450
EKVFKTFTAM PEYQRWTQRH LGAVGAQRLY AMLATQLFIP AAFSNVYFIG
460 470 480 490 500
GQEIGDFLMR GAYLSGVGNY VALCFCSYCF FQCSELLLTK SDGRSKTKTF
Length:500
Mass (Da):58,106
Last modified:May 1, 2000 - v1
Checksum:i4498DC306976F2A2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti91G → V (PubMed:11509241).Curated1
Sequence conflicti91G → V (PubMed:12537569).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF393157 mRNA Translation: AAK73748.1
AF398410 mRNA Translation: AAK97480.1
AE014296 Genomic DNA Translation: AAF47725.1
AY119202 mRNA Translation: AAM51062.1
RefSeqiNP_523898.1, NM_079174.2
UniGeneiDm.2681

Genome annotation databases

EnsemblMetazoaiFBtr0073029; FBpp0072893; FBgn0024194
GeneIDi44098
KEGGidme:Dmel_CG11495

Similar proteinsi

Entry informationi

Entry nameiHHAT_DROME
AccessioniPrimary (citable) accession number: Q9VZU2
Secondary accession number(s): Q95VY0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 24, 2003
Last sequence update: May 1, 2000
Last modified: July 18, 2018
This is version 117 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

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