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UniProtKB - Q9VY99 (NNAD_DROME)
Protein
Cytosolic carboxypeptidase Nna1
Gene
Nna1
Organism
Drosophila melanogaster (Fruit fly)
Status
Functioni
Metallocarboxypeptidase.
By similarityCofactori
Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 938 | ZincBy similarity | 1 | |
Metal bindingi | 941 | ZincBy similarity | 1 | |
Active sitei | 988 | NucleophileBy similarity | 1 | |
Metal bindingi | 1034 | ZincBy similarity | 1 |
GO - Molecular functioni
- metallocarboxypeptidase activity Source: InterPro
- zinc ion binding Source: InterPro
GO - Biological processi
- larval development Source: UniProtKB
- mitochondrion organization Source: UniProtKB
- protein side chain deglutamylation Source: GO_Central
Keywordsi
Molecular function | Carboxypeptidase, Hydrolase, Metalloprotease, Protease |
Ligand | Metal-binding, Zinc |
Protein family/group databases
MEROPSi | M14.A21 |
Names & Taxonomyi
Protein namesi | Recommended name: Cytosolic carboxypeptidase Nna1Curated (EC:3.4.17.-)Alternative name(s): Nervous system nuclear protein induced by axotomy protein 1 homolog |
Gene namesi | ORF Names:CG44533Imported |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0265726, Nna1 |
VEuPathDBi | VectorBase:FBgn0265726 |
Subcellular locationi
Mitochondrion
- Mitochondrion 1 Publication
Mitochondrion
- mitochondrion Source: UniProtKB
Keywords - Cellular componenti
MitochondrionPathology & Biotechi
Disruption phenotypei
Semilethal phenotype: females survive, while males die. Most male flies die between the late second instar larval stage and early pupal stage. Mutant males that survive to produce pupal cases initiate morphogenesis but die before head eversion. Almost all male flies that survive past head eversion do complete morphogenesis, and then eclose as 'escaper' males. Escapers display a range of phenotypes that mirror the disease pathology observed in pcd mice, including retinal degeneration.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000403765 | 1 – 1201 | Cytosolic carboxypeptidase Nna1Add BLAST | 1201 |
Proteomic databases
PaxDbi | Q9VY99 |
PRIDEi | Q9VY99 |
Expressioni
Tissue specificityi
In larvae, highly expressed in the brain, optic lobes, and ventral ganglia.1 Publication
Developmental stagei
Expressed in larvae and adults.1 Publication
Gene expression databases
Bgeei | FBgn0265726, Expressed in adult organism and 26 other tissues |
ExpressionAtlasi | Q9VY99, baseline and differential |
Genevisiblei | Q9VY99, DM |
Interactioni
Protein-protein interaction databases
BioGRIDi | 58706, 2 interactors |
IntActi | Q9VY99, 1 interactor |
STRINGi | 7227.FBpp0073644 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 63 – 113 | DisorderedSequence analysisAdd BLAST | 51 | |
Regioni | 255 – 303 | DisorderedSequence analysisAdd BLAST | 49 | |
Regioni | 362 – 405 | DisorderedSequence analysisAdd BLAST | 44 | |
Regioni | 474 – 526 | DisorderedSequence analysisAdd BLAST | 53 | |
Regioni | 544 – 574 | DisorderedSequence analysisAdd BLAST | 31 | |
Regioni | 623 – 651 | DisorderedSequence analysisAdd BLAST | 29 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 70 – 97 | Basic and acidic residuesSequence analysisAdd BLAST | 28 | |
Compositional biasi | 255 – 275 | Basic and acidic residuesSequence analysisAdd BLAST | 21 | |
Compositional biasi | 383 – 400 | Basic and acidic residuesSequence analysisAdd BLAST | 18 | |
Compositional biasi | 502 – 518 | Acidic residuesSequence analysisAdd BLAST | 17 | |
Compositional biasi | 635 – 651 | Polar residuesSequence analysisAdd BLAST | 17 |
Sequence similaritiesi
Belongs to the peptidase M14 family.Curated
Phylogenomic databases
eggNOGi | KOG3641, Eukaryota |
InParanoidi | Q9VY99 |
PhylomeDBi | Q9VY99 |
Family and domain databases
InterProi | View protein in InterPro IPR040626, Pepdidase_M14_N IPR000834, Peptidase_M14 |
Pfami | View protein in Pfam PF18027, Pepdidase_M14_N, 1 hit PF00246, Peptidase_M14, 1 hit |
s (5+)i Sequence
Sequence statusi: Complete.
This entry describes 5 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 5 described isoforms and 6 potential isoforms that are computationally mapped.Show allAlign All
Isoform A (identifier: Q9VY99-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAEREREAMS ASLSRLLRTL SSASMSRRHC PLALLQPIPL HQSEIALGSA
60 70 80 90 100
SSGGSAERCC SSCSDDSNNN ERCKSAERGT AADEVGEEGR EEVELESKED
110 120 130 140 150
AVEGEQDELE LKRDSSTVNV NVVRANFKCN DMECSLVLGD YVNGFLGSFL
160 170 180 190 200
SKGLKTNQLV VNTDEKTLRP VARLKEPRDL FALPKDKDND CSQQAPRWPV
210 220 230 240 250
ECQVIEERII HIPYVPAVPE PLNAPTGNEL KPRPVGEENG IVVFSYSPIS
260 270 280 290 300
AVNYEKPKAK KEDDESSDES DYSSDSRQDS TPPSRSTPMR LAGGGECAPG
310 320 330 340 350
KLNSVSKMIN NVDNRSTSAS LDDNDDYYDD EYDTSGGYCG GSGEAKEKAI
360 370 380 390 400
IKDLIEAKKK RYQEEAEVTP VGGGTARNSR AASRSEASKD ASDIDDIWKN
410 420 430 440 450
NTSEYKPASP RYVLSQFGKN VTKAVIDRIV DHEDLVPPSG SQKVSNQFAV
460 470 480 490 500
GPVKLPKTNI ARLEVAFERS ACQDRSKSRL KKKMSSSRRR RESTSDEDSS
510 520 530 540 550
SSSLGNEEDE EVDDSDSDAE NTGSGIGLRR ILGSYSARLA GGAEKCPCPG
560 570 580 590 600
SGSVSDTETL VGDESRSRLA GSNGLHQQDV VCSRNRQAQS RSPLRSVPHT
610 620 630 640 650
HAATVAAAAA ALARGRNRDK DGCLGGKDEK NMGMGVGTTG TSSMGTRTSS
660 670 680 690 700
PVRNNVFRLS KDQHILLNSM TSQETTGTLI SSTSTNQTTT TTNSSQSFLC
710 720 730 740 750
SDLPQAQFSR SAVGGARFMT NCHPMNPEEY DGLEFESRFE SGNLAKAVQI
760 770 780 790 800
TPTYYELYLR PDLYTSRSKQ WFYFRVRRTR RKMLYRFSIV NLVKSDSLYN
810 820 830 840 850
DGMQPVMYST LGAKEKSEGW RRCGDNICYY RNDDESASSS ANEDDEDNST
860 870 880 890 900
YTLTFTIEFE HDDDTVFFAH SYPYTYSDLQ DYLMEIQRHP VKSKFCKLRL
910 920 930 940 950
LCRTLAGNNV YYLTVTAPSS NEENMRRKKS IVVSARVHPS ETPASWMMKG
960 970 980 990 1000
LMDFITGDTT VAKRLRHKFI FKLVPMLNPD GVIVGNTRNS LTGKDLNRQY
1010 1020 1030 1040 1050
RTVIRETYPS IWYTKAMIRR LIEECGVAMY CDMHAHSRKH NIFIYGCENK
1060 1070 1080 1090 1100
RNPEKKLTEQ VFPLMLHKNS ADRFSFESCK FKIQRSKEGT GRIVVWMLGI
1110 1120 1130 1140 1150
TNSYTIEASF GGSSLGSRKG THFNTQDYEH MGRAFCETLL DYCDENPNKV
1160 1170 1180 1190 1200
KRHAKLFKQI KKIRKREKRE QKALKLQKMA DQGCIMNDKL KVKRSVEKSV
S
Computationally mapped potential isoform sequencesi
There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketM9NEF7 | M9NEF7_DROME | Nna1 carboxypeptidase, isoform F | Nna1 CG11428, CG32627, CG32628, Dmel\CG44533, NnaD | 913 | Annotation score: | ||
M9NF40 | M9NF40_DROME | Nna1 carboxypeptidase, isoform G | Nna1 CG11428, CG32627, CG32628, Dmel\CG44533, NnaD | 748 | Annotation score: | ||
X2JBR4 | X2JBR4_DROME | Nna1 carboxypeptidase, isoform H | Nna1 CG11428, CG32627, CG32628, Dmel\CG44533, NnaD | 1,890 | Annotation score: | ||
M9NGD4 | M9NGD4_DROME | Nna1 carboxypeptidase, isoform E | Nna1 CG11428, CG32627, CG32628, Dmel\CG44533, NnaD | 1,089 | Annotation score: | ||
X2JDN6 | X2JDN6_DROME | Nna1 carboxypeptidase, isoform I | Nna1 CG11428, CG32627, CG32628, Dmel\CG44533, NnaD | 1,889 | Annotation score: | ||
X2JK43 | X2JK43_DROME | Nna1 carboxypeptidase, isoform J | Nna1 CG11428, CG32627, CG32628, Dmel\CG44533, NnaD | 1,694 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_040439 | 1 – 288 | Missing in isoform C. CuratedAdd BLAST | 288 | |
Alternative sequenceiVSP_040440 | 1 – 143 | MAERE…GDYVN → MDFGGGRVQFLQAQLFPVCT LTTSSG in isoform D. CuratedAdd BLAST | 143 | |
Alternative sequenceiVSP_040441 | 1 – 143 | MAERE…GDYVN → MQKGVKDKENRNSAMGPEIC EVQQHPSKQND in isoform E. CuratedAdd BLAST | 143 | |
Alternative sequenceiVSP_040442 | 444 – 707 | Missing in isoform B. 1 PublicationAdd BLAST | 264 | |
Alternative sequenceiVSP_040443 | 1183 – 1201 | GCIMN…EKSVS → ILNLLKQQDRLRSKIIERLM REGSSADEPLNIPLSDYSSD EGNCSSSSDNEGKHSITTSD LEGPCCAPTRAPPSSPEVIH EIRKFRMRRMRKVMHELDRI YFTPLFQRKFKTLTTLKRRR QKMGVKAAPSGKRLRGGGGP AVSAATPTPAPAVQAAAPVV ETNDVQKLHTPRQLARSLPS NSDQPTGGQSSDSSESMDSS QSESLLEPDGSSGSTAASKE VTKKVKTSGAGTKKTAKKKK FMPTEKKKPVVNQKLHVDRN FRLWLANRRIYIYRRKKSTQ ARRTKVRNKPSKKRGEVVRT TLDLPTTDPGSDLHFSTDDE EHSPTSGHNGYGAVPPLRHT LLQSDLQRRYIEEIGDTVVQ KPKASHMPPELIVTTPSKSG TPGGGKKLDVYKLTPRTAPE LDTGIGMMQRQAGGTGTSAR RTYSWHNLDQQEIPNGNGGG QGKANFYIGDTKPGIKTMAK PLPRRSVPSNFIPQRHGNVQ TADDLQLKLSLKKKVWTGAN GDADGRPLAWYKGHSITASQ MANTTTTIRSAGGGAAGGGG AGAGAGAGGGGGGQNRNMFP SNGREQTAASGGIAMGVPPA FVGAPRRFRKLEQVDLFNAC SQKLMMWQQQEEQRRSHPQQ AQRLLKVEDHPQHSRDREQQ PFKPMHVVRKSTGTTSQAKV IQALVAAESGGKVKRKSSSM MKIAETTQLVTRFARNRNSA GGSAAQQQQQQQPPQHMHHQ HKRLLFKGQGGVGAMGSRMH SAGVMGNMQGNGGGRAPNKF KTGGLVITAVQQPTNMTGGG SRRMRNAAGLQAKGSNGALG SSTGQMQYQRSNASVQANKS ATEISMDAVSLVRKVKTKLK KRKSRTLSTGAPK in isoform E. CuratedAdd BLAST | 19 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE014298 Genomic DNA Translation: AAF48304.2 AE014298 Genomic DNA Translation: AAN09334.1 AE014298 Genomic DNA Translation: AAO41651.1 AE014298 Genomic DNA Translation: ABW09409.1 AY069812 mRNA Translation: AAL39957.1 BT099687 mRNA Translation: ACV44473.1 |
RefSeqi | NP_001096975.1, NM_001103505.3 [Q9VY99-4] NP_001245656.1, NM_001258727.1 [Q9VY99-3] NP_572914.2, NM_132686.3 [Q9VY99-1] NP_727728.1, NM_167379.2 [Q9VY99-2] NP_788899.1, NM_176726.2 [Q9VY99-3] |
Genome annotation databases
EnsemblMetazoai | FBtr0340671; FBpp0309513; FBgn0265726 [Q9VY99-1] FBtr0340672; FBpp0309514; FBgn0265726 [Q9VY99-2] FBtr0340673; FBpp0309515; FBgn0265726 [Q9VY99-3] FBtr0340674; FBpp0309516; FBgn0265726 [Q9VY99-4] FBtr0340676; FBpp0309518; FBgn0265726 [Q9VY99-3] |
GeneIDi | 32329 |
KEGGi | dme:Dmel_CG44533 |
UCSCi | CG32627-RA, d. melanogaster [Q9VY99-1] CG32627-RB, d. melanogaster CG32627-RC, d. melanogaster CG32627-RD, d. melanogaster |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE014298 Genomic DNA Translation: AAF48304.2 AE014298 Genomic DNA Translation: AAN09334.1 AE014298 Genomic DNA Translation: AAO41651.1 AE014298 Genomic DNA Translation: ABW09409.1 AY069812 mRNA Translation: AAL39957.1 BT099687 mRNA Translation: ACV44473.1 |
RefSeqi | NP_001096975.1, NM_001103505.3 [Q9VY99-4] NP_001245656.1, NM_001258727.1 [Q9VY99-3] NP_572914.2, NM_132686.3 [Q9VY99-1] NP_727728.1, NM_167379.2 [Q9VY99-2] NP_788899.1, NM_176726.2 [Q9VY99-3] |
3D structure databases
AlphaFoldDBi | Q9VY99 |
SMRi | Q9VY99 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 58706, 2 interactors |
IntActi | Q9VY99, 1 interactor |
STRINGi | 7227.FBpp0073644 |
Protein family/group databases
MEROPSi | M14.A21 |
Proteomic databases
PaxDbi | Q9VY99 |
PRIDEi | Q9VY99 |
Protocols and materials databases
DNASUi | 32329 |
Genome annotation databases
EnsemblMetazoai | FBtr0340671; FBpp0309513; FBgn0265726 [Q9VY99-1] FBtr0340672; FBpp0309514; FBgn0265726 [Q9VY99-2] FBtr0340673; FBpp0309515; FBgn0265726 [Q9VY99-3] FBtr0340674; FBpp0309516; FBgn0265726 [Q9VY99-4] FBtr0340676; FBpp0309518; FBgn0265726 [Q9VY99-3] |
GeneIDi | 32329 |
KEGGi | dme:Dmel_CG44533 |
UCSCi | CG32627-RA, d. melanogaster [Q9VY99-1] CG32627-RB, d. melanogaster CG32627-RC, d. melanogaster CG32627-RD, d. melanogaster |
Organism-specific databases
CTDi | 32329 |
FlyBasei | FBgn0265726, Nna1 |
VEuPathDBi | VectorBase:FBgn0265726 |
Phylogenomic databases
eggNOGi | KOG3641, Eukaryota |
InParanoidi | Q9VY99 |
PhylomeDBi | Q9VY99 |
Miscellaneous databases
BioGRID-ORCSi | 32329, 0 hits in 3 CRISPR screens |
GenomeRNAii | 32329 |
PROi | PR:Q9VY99 |
Gene expression databases
Bgeei | FBgn0265726, Expressed in adult organism and 26 other tissues |
ExpressionAtlasi | Q9VY99, baseline and differential |
Genevisiblei | Q9VY99, DM |
Family and domain databases
InterProi | View protein in InterPro IPR040626, Pepdidase_M14_N IPR000834, Peptidase_M14 |
Pfami | View protein in Pfam PF18027, Pepdidase_M14_N, 1 hit PF00246, Peptidase_M14, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | NNAD_DROME | |
Accessioni | Q9VY99Primary (citable) accession number: Q9VY99 Secondary accession number(s): A8JUW0 Q8T9D7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 11, 2011 |
Last sequence update: | October 1, 2002 | |
Last modified: | May 25, 2022 | |
This is version 135 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - SIMILARITY comments
Index of protein domains and families