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Entry version 131 (02 Jun 2021)
Sequence version 1 (01 May 2000)
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Protein

GATOR complex protein NPRL2

Gene

Nprl2

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

An essential component of the GATOR subcomplex GATOR1 which functions as an inhibitor of the amino acid-sensing branch of the TORC1 signaling pathway (PubMed:23723238, PubMed:27166823, PubMed:25512509).

The two GATOR subcomplexes, GATOR1 and GATOR2, regulate the TORC1 pathway in order to mediate metabolic homeostasis, female gametogenesis and the response to amino acid limitation and complete starvation (PubMed:23723238, PubMed:27166823, PubMed:25512509).

The function of GATOR1 in negatively regulating the TORC1 pathway is essential for maintaining baseline levels of TORC1 activity under nutrient rich conditions, and for promoting survival during amino acid or complete starvation by inhibiting TORC1-dependent cell growth and promoting catabolic metabolism and autophagy (PubMed:23723238, PubMed:27166823).

In addition, this inhibition of TORC1 is necessary to maintain female fertility under normal conditions and during periods of nutrient stress (PubMed:24786828, PubMed:27672113, PubMed:25512509).

GATOR1 and GATOR2 act at different stages of oogenesis to regulate TORC1 in order to control meiotic entry and promote oocyte growth and development (PubMed:25512509).

After exactly four mitotic cyst divisions, the GATOR1 complex members (Iml1, Nprl2 and Nprl3) down-regulate TORC1 to slow cellular metabolism and promote the mitotic/meiotic transition (PubMed:25512509).

At later stages of oogenesis, the mio and Nup44A components of the GATOR2 complex inhibit GATOR1 and thus activate TORC1 to promote meiotic progression, and drive oocyte growth and development (PubMed:25512509).

5 Publications

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Biological processCell cycle, Cell division, Meiosis, Mitosis

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
GATOR complex protein NPRL2Curated
Alternative name(s):
Nitrogen permease regulator 2-like proteinImported
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:Nprl2Imported
ORF Names:CG9104Imported
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiDrosophila melanogaster (Fruit fly)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri7227 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000803 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome X

Organism-specific databases

Drosophila genome database

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FlyBasei
FBgn0030800, Nprl2

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Keywords - Cellular componenti

Cytoplasm, Lysosome

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This subsection of the 'Pathology and Biotech' section describes the in vivo effects caused by ablation of the gene (or one or more transcripts) coding for the protein described in the entry. This includes gene knockout and knockdown, provided experiments have been performed in the context of a whole organism or a specific tissue, and not at the single-cell level.<p><a href='/help/disruption_phenotype' target='_top'>More...</a></p>Disruption phenotypei

Pupal homozygous semi-lethal (PubMed:27672113). Under nutrient-replete conditions, larvae display a significant increase in TORC1 activity and adult escapers display a small, but significant increase in body weight and a reduction in climbing (PubMed:27672113). Newly hatched males display decreased tolerance to both complete starvation and amino acid starvation, likely due to decreased triacylglyceride (TAG) storage and the inability to downregulate TORC1 activity and activate catabolic metabolism and autophagy (PubMed:27672113). Conditional RNAi-mediated knockdown in the female germline results in a small decrease in the rate of egg production when females are provided with a protein source of wet yeast (PubMed:24786828). Females starved of amino acids for a brief period have increased numbers of degenerating young eggs and show permanent loss of fertility (PubMed:24786828). Mid-stage egg chambers are unaffected (PubMed:24786828). Double RNAi-mediated knockdown with another GATOR1 complex member Iml1 in the female germline, increases the penetrance of ovarian cysts displaying delayed mitotic exit and producing 32-cell cysts (PubMed:25512509).3 Publications

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00004353151 – 412GATOR complex protein NPRL2Add BLAST412

Proteomic databases

PaxDb, a database of protein abundance averages across all three domains of life

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PaxDbi
Q9VXA0

PRoteomics IDEntifications database

More...
PRIDEi
Q9VXA0

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Bgee dataBase for Gene Expression Evolution

More...
Bgeei
FBgn0030800, Expressed in saliva-secreting gland and 34 other tissues

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Component of the GATOR complex consisting of mio, Nup44A/Seh1, Im11, Nplr3, Nplr2, Wdr24, Wdr59 and Sec13 (PubMed:27166823). Within the GATOR complex, probable component of the GATOR1 subcomplex which is likely composed of Iml1, Nplr2 and Nplr3 (PubMed:27166823).

Interacts with Nprl3 (PubMed:24786828).

2 Publications

Protein-protein interaction databases

STRING: functional protein association networks

More...
STRINGi
7227.FBpp0074122

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the NPR2 family.Curated

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

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eggNOGi
KOG3789, Eukaryota

Ensembl GeneTree

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GeneTreei
ENSGT00390000001414

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

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HOGENOMi
CLU_014995_0_0_1

InParanoid: Eukaryotic Ortholog Groups

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InParanoidi
Q9VXA0

Identification of Orthologs from Complete Genome Data

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OMAi
YASMTHG

Database of Orthologous Groups

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OrthoDBi
500740at2759

Database for complete collections of gene phylogenies

More...
PhylomeDBi
Q9VXA0

Family and domain databases

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR009348, NPR2

The PANTHER Classification System

More...
PANTHERi
PTHR12991, PTHR12991, 1 hit

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF06218, NPR2, 2 hits

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

Q9VXA0-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MHSHTNETAA TSTTAGAVTN GAGGSGAGAA GAHGSTEGKI RCIFLSEFHA
60 70 80 90 100
TAGCKISCQV PDNYISKDVF DAINVYIIPK QHLQRCILTV NAMDVKIVGY
110 120 130 140 150
PVGIQDQQKY ARNAFLFNLC FVCDSRARSV QYEPVVKKLS EYLIMMEEES
160 170 180 190 200
CFLSREDDKR RLQNIFETVL RDLNERKVAT IVEGDNTIYL KIVMHKPDPP
210 220 230 240 250
PVKDHMVPLL LANLRDAPLD NWDLTTQQIL PYINGINHVA RIAAEADVET
260 270 280 290 300
DLVKSCIQNL VYYGVVQLLP ILKYSNVYMT QNLKHLIQSA SLSGACRKYV
310 320 330 340 350
ALRPDKTLPS VQRIFQFYAS MTHGVTLRAI CQRLCPQHHN IDERRMVIFG
360 370 380 390 400
LQHRFIRCIH KYPVFTGSVP SGRQKMYTGL ISFDEICCKT GLSPCTIERD
410
IEKDTNVTVI WK
Length:412
Mass (Da):46,345
Last modified:May 1, 2000 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iD11544CA83338F10
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

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EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

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DDBJi
Links Updated
AE003503 Genomic DNA Translation: AAF48677.1
AY051914 mRNA Translation: AAK93338.1

NCBI Reference Sequences

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RefSeqi
NP_573174.1, NM_132946.3

Genome annotation databases

Ensembl metazoan genome annotation project

More...
EnsemblMetazoai
FBtr0074348; FBpp0074122; FBgn0030800

Database of genes from NCBI RefSeq genomes

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GeneIDi
32677

KEGG: Kyoto Encyclopedia of Genes and Genomes

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KEGGi
dme:Dmel_CG9104

UCSC genome browser

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UCSCi
CG9104-RA, d. melanogaster

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE003503 Genomic DNA Translation: AAF48677.1
AY051914 mRNA Translation: AAK93338.1
RefSeqiNP_573174.1, NM_132946.3

3D structure databases

Database of comparative protein structure models

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ModBasei
Search...

SWISS-MODEL Interactive Workspace

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SWISS-MODEL-Workspacei
Submit a new modelling project...

Protein-protein interaction databases

STRINGi7227.FBpp0074122

Proteomic databases

PaxDbiQ9VXA0
PRIDEiQ9VXA0

Protocols and materials databases

The DNASU plasmid repository

More...
DNASUi
32677

Genome annotation databases

EnsemblMetazoaiFBtr0074348; FBpp0074122; FBgn0030800
GeneIDi32677
KEGGidme:Dmel_CG9104
UCSCiCG9104-RA, d. melanogaster

Organism-specific databases

Comparative Toxicogenomics Database

More...
CTDi
10641
FlyBaseiFBgn0030800, Nprl2

Phylogenomic databases

eggNOGiKOG3789, Eukaryota
GeneTreeiENSGT00390000001414
HOGENOMiCLU_014995_0_0_1
InParanoidiQ9VXA0
OMAiYASMTHG
OrthoDBi500740at2759
PhylomeDBiQ9VXA0

Miscellaneous databases

BioGRID ORCS database of CRISPR phenotype screens

More...
BioGRID-ORCSi
32677, 0 hits in 1 CRISPR screen

Database of phenotypes from RNA interference screens in Drosophila and Homo sapiens

More...
GenomeRNAii
32677

Protein Ontology

More...
PROi
PR:Q9VXA0

Gene expression databases

BgeeiFBgn0030800, Expressed in saliva-secreting gland and 34 other tissues

Family and domain databases

InterProiView protein in InterPro
IPR009348, NPR2
PANTHERiPTHR12991, PTHR12991, 1 hit
PfamiView protein in Pfam
PF06218, NPR2, 2 hits

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiNPRL2_DROME
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q9VXA0
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 20, 2016
Last sequence update: May 1, 2000
Last modified: June 2, 2021
This is version 131 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families
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