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Protein

ATP-dependent (S)-NAD(P)H-hydrate dehydratase

Gene

CG10424

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ATP, which is converted to ADP. Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.UniRule annotation

Catalytic activityi

ATP + (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide = ADP + phosphate + NADH.UniRule annotation
ATP + (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate = ADP + phosphate + NADPH.

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei114NAD(P)HX; via amide nitrogenUniRule annotation1
Binding sitei229NAD(P)HXUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi198 – 202ATPUniRule annotation5
Nucleotide bindingi219 – 228ATPUniRule annotation10

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
LigandATP-binding, NAD, NADP, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DME-197264 Nicotinamide salvaging

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent (S)-NAD(P)H-hydrate dehydrataseUniRule annotation (EC:4.2.1.93UniRule annotation)
Alternative name(s):
ATP-dependent NAD(P)HX dehydrataseUniRule annotation
Gene namesi
ORF Names:CG10424
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0036848 CG10424

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004161651 – 300ATP-dependent (S)-NAD(P)H-hydrate dehydrataseAdd BLAST300

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9VVW8
PRIDEiQ9VVW8

Expressioni

Gene expression databases

BgeeiFBgn0036848 Expressed in 35 organ(s), highest expression level in arthropod fat body
GenevisibleiQ9VVW8 DM

Interactioni

Protein-protein interaction databases

BioGridi65362, 1 interactor
IntActiQ9VVW8, 2 interactors
STRINGi7227.FBpp0074800

Structurei

3D structure databases

ProteinModelPortaliQ9VVW8
SMRiQ9VVW8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini14 – 293YjeF C-terminalUniRule annotationAdd BLAST280

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni167 – 173NAD(P)HXUniRule annotation7

Sequence similaritiesi

Belongs to the NnrD/CARKD family.UniRule annotation

Phylogenomic databases

eggNOGiKOG3974 Eukaryota
COG0063 LUCA
GeneTreeiENSGT00390000000917
InParanoidiQ9VVW8
KOiK17757
OMAiNLMVHPL
OrthoDBiEOG091G0CC8
PhylomeDBiQ9VVW8

Family and domain databases

CDDicd01171 YXKO-related, 1 hit
Gene3Di3.40.1190.20, 1 hit
HAMAPiMF_01965 NADHX_dehydratase, 1 hit
InterProiView protein in InterPro
IPR017953 Carbohydrate_kinase_pred_CS
IPR000631 CARKD
IPR029056 Ribokinase-like
PfamiView protein in Pfam
PF01256 Carb_kinase, 1 hit
SUPFAMiSSF53613 SSF53613, 1 hit
TIGRFAMsiTIGR00196 yjeF_cterm, 1 hit
PROSITEiView protein in PROSITE
PS01049 YJEF_C_1, 1 hit
PS51383 YJEF_C_3, 1 hit

Sequencei

Sequence statusi: Complete.

Q9VVW8-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAAVSDIPVH LPKLLALFKT VVPKLVNNKH KGQYGRIGVI GGSLEYTGAP
60 70 80 90 100
YFAAISSIRV GADLAHVFCH SNASAIIKSY SPDLIVHPVL DCVDAVERIA
110 120 130 140 150
PWLERLHVVV IGPGLGREPG ILKTASNVLK LCMDTKKPVV IDADGLFLLN
160 170 180 190 200
DNLNLICGQP NVILTPNVME FQRLFGEDDQ AARQKMSLLG AGVTVLEKGA
210 220 230 240 250
NDKIYLPHCN EVHSMPSGGS GRRCGGQGDL LSGSLATFFS WSLQSGEPNP
260 270 280 290 300
ALVAACASSY FVKKLNAAAF QKFGRSLLAS DMVNQIPSVF QTEFENSDPQ
Length:300
Mass (Da):32,153
Last modified:May 1, 2000 - v1
Checksum:iE6A237B4978A8599
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014296 Genomic DNA Translation: AAF49189.1
AY060665 mRNA Translation: AAL28213.1
RefSeqiNP_649090.1, NM_140833.3
UniGeneiDm.3917

Genome annotation databases

EnsemblMetazoaiFBtr0075033; FBpp0074800; FBgn0036848
GeneIDi40085
KEGGidme:Dmel_CG10424
UCSCiCG10424-RA d. melanogaster

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014296 Genomic DNA Translation: AAF49189.1
AY060665 mRNA Translation: AAL28213.1
RefSeqiNP_649090.1, NM_140833.3
UniGeneiDm.3917

3D structure databases

ProteinModelPortaliQ9VVW8
SMRiQ9VVW8
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi65362, 1 interactor
IntActiQ9VVW8, 2 interactors
STRINGi7227.FBpp0074800

Proteomic databases

PaxDbiQ9VVW8
PRIDEiQ9VVW8

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0075033; FBpp0074800; FBgn0036848
GeneIDi40085
KEGGidme:Dmel_CG10424
UCSCiCG10424-RA d. melanogaster

Organism-specific databases

FlyBaseiFBgn0036848 CG10424

Phylogenomic databases

eggNOGiKOG3974 Eukaryota
COG0063 LUCA
GeneTreeiENSGT00390000000917
InParanoidiQ9VVW8
KOiK17757
OMAiNLMVHPL
OrthoDBiEOG091G0CC8
PhylomeDBiQ9VVW8

Enzyme and pathway databases

ReactomeiR-DME-197264 Nicotinamide salvaging

Miscellaneous databases

GenomeRNAii40085
PROiPR:Q9VVW8

Gene expression databases

BgeeiFBgn0036848 Expressed in 35 organ(s), highest expression level in arthropod fat body
GenevisibleiQ9VVW8 DM

Family and domain databases

CDDicd01171 YXKO-related, 1 hit
Gene3Di3.40.1190.20, 1 hit
HAMAPiMF_01965 NADHX_dehydratase, 1 hit
InterProiView protein in InterPro
IPR017953 Carbohydrate_kinase_pred_CS
IPR000631 CARKD
IPR029056 Ribokinase-like
PfamiView protein in Pfam
PF01256 Carb_kinase, 1 hit
SUPFAMiSSF53613 SSF53613, 1 hit
TIGRFAMsiTIGR00196 yjeF_cterm, 1 hit
PROSITEiView protein in PROSITE
PS01049 YJEF_C_1, 1 hit
PS51383 YJEF_C_3, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNNRD_DROME
AccessioniPrimary (citable) accession number: Q9VVW8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 21, 2012
Last sequence update: May 1, 2000
Last modified: October 10, 2018
This is version 132 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
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Main funding by: National Institutes of Health

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