UniProtKB - Q9VVT2 (INDY1_DROME)
Protein I'm not dead yet
Indy
Functioni
Cation-independent electroneutral transporter (not associated with membrane depolarization) of a variety of tricarboxylic and dicarboxylic acid-cycle intermediates. There is also small, but detectable, transport of monocarboxylics. Transport is through the epithelium of the gut and across the plasma membranes of organs involved in intermediary metabolism and storage. Affinity for substrates is citrate > succinate > pyruvate. Fumarate, a-ketoglutarate, and glutarate are also transported, but not lactate. Transport mechanism that is not coupled to Na+, K+, or Cl-. Function is shown in Xenopus oocytes and human retinal pigment epithelial (HRPE) cell lines.
3 PublicationsMiscellaneous
GO - Molecular functioni
- citrate transmembrane transporter activity Source: UniProtKB
- pyruvate transmembrane transporter activity Source: UniProtKB
- succinate transmembrane transporter activity Source: UniProtKB
GO - Biological processi
- anion transmembrane transport Source: GO_Central
- citrate transport Source: UniProtKB
- determination of adult lifespan Source: FlyBase
- pyruvate transport Source: UniProtKB
- regulation of sequestering of triglyceride Source: FlyBase
- succinate transport Source: UniProtKB
Keywordsi
Biological process | Transport |
Enzyme and pathway databases
Reactomei | R-DME-433137, Sodium-coupled sulphate, di- and tri-carboxylate transporters |
SignaLinki | Q9VVT2 |
Protein family/group databases
TCDBi | 2.A.47.1.10, the divalent anion:na(+) symporter (dass) family |
Names & Taxonomyi
Protein namesi | Recommended name: Protein I'm not dead yetAlternative name(s): INDY transporter protein drIndy |
Gene namesi | Name:Indy ORF Names:CG3979 |
Organismi | Drosophila melanogaster (Fruit fly)Imported |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0036816, Indy |
VEuPathDBi | VectorBase:FBgn0036816 |
Subcellular locationi
Plasma membrane
- Basolateral cell membrane 1 Publication; Multi-pass membrane protein 1 Publication
Note: Basolateral membrane of cells in the midgut.
Plasma Membrane
- basolateral plasma membrane Source: UniProtKB-SubCell
- integral component of plasma membrane Source: UniProtKB
- plasma membrane Source: FlyBase
Other locations
- integral component of membrane Source: GO_Central
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 43 – 63 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 82 – 102 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 115 – 135 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 153 – 173 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 224 – 244 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 270 – 290 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 329 – 349 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 373 – 393 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 457 – 477 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 509 – 529 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 540 – 560 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000172499 | 1 – 590 | Protein I'm not dead yetAdd BLAST | 590 |
Proteomic databases
PaxDbi | Q9VVT2 |
PRIDEi | Q9VVT2 |
PTM databases
SwissPalmi | Q9VVT2 |
Expressioni
Tissue specificityi
Inductioni
Gene expression databases
Bgeei | FBgn0036816, Expressed in arthropod fat body and 46 other tissues |
ExpressionAtlasi | Q9VVT2, baseline and differential |
Genevisiblei | Q9VVT2, DM |
Interactioni
Protein-protein interaction databases
BioGRIDi | 65330, 4 interactors |
DIPi | DIP-18443N |
IntActi | Q9VVT2, 3 interactors |
STRINGi | 7227.FBpp0074848 |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1281, Eukaryota |
GeneTreei | ENSGT01030000234550 |
InParanoidi | Q9VVT2 |
PhylomeDBi | Q9VVT2 |
Family and domain databases
InterProi | View protein in InterPro IPR031312, Na/sul_symport_CS IPR001898, SLC13A/DASS |
Pfami | View protein in Pfam PF00939, Na_sulph_symp, 1 hit |
PROSITEi | View protein in PROSITE PS01271, NA_SULFATE, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MATETTKMIY TPPPLDIKME IEIGEQPQPP VKCSNFFANH WKGLVVFLVP
60 70 80 90 100
LLCLPVMLLN EGAEFRCMYL LLVMAIFWVT EALPLYVTSM IPIVAFPIMG
110 120 130 140 150
IMSSDQTCRL YFKDTLVMFM GGIMVALAVE YCNLHKRLAL RVIQIVGCSP
160 170 180 190 200
RRLHFGLIMV TMFLSMWISN AACTAMMCPI IQAVLEELQA QGVCKINHEP
210 220 230 240 250
QYQIVGGNKK NNEDEPPYPT KITLCYYLGI AYASSLGGCG TIIGTATNLT
260 270 280 290 300
FKGIYEARFK NSTEQMDFPT FMFYSVPSML VYTLLTFVFL QWHFMGLWRP
310 320 330 340 350
KSKEAQEVQR GREGADVAKK VIDQRYKDLG PMSIHEIQVM ILFIFMVVMY
360 370 380 390 400
FTRKPGIFLG WADLLNSKDI RNSMPTIFVV VMCFMLPANY AFLRYCTRRG
410 420 430 440 450
GPVPTGPTPS LITWKFIQTK VPWGLVFLLG GGFALAEGSK QSGMAKLIGN
460 470 480 490 500
ALIGLKVLPN SVLLLVVILV AVFLTAFSSN VAIANIIIPV LAEMSLAIEI
510 520 530 540 550
HPLYLILPAG LACSMAFHLP VSTPPNALVA GYANIRTKDM AIAGIGPTII
560 570 580 590
TIITLFVFCQ TWGLVVYPNL NSFPEWAQIY AAAALGNKTH
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketE1JI19 | E1JI19_DROME | I'm not dead yet, isoform D | Indy anon-EST:fe3A7, anon-WO0172774.70, BEST:LP01220, Dmel\CG3979, drIndy | 572 | Annotation score: |
Sequence cautioni
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_029170 | 1 – 18 | Missing in isoform B. CuratedAdd BLAST | 18 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF509505 mRNA Translation: AAN86815.1 AE014296 Genomic DNA Translation: AAF49226.1 AE014296 Genomic DNA Translation: AAF49227.2 AE014296 Genomic DNA Translation: AAN11670.1 AY102686 mRNA Translation: AAM27515.1 AF217399 mRNA Translation: AAF73384.1 Different initiation. |
RefSeqi | NP_001163465.1, NM_001169994.2 [Q9VVT2-2] NP_524150.1, NM_079426.4 [Q9VVT2-2] NP_730363.2, NM_168778.2 [Q9VVT2-1] NP_730364.1, NM_168779.2 [Q9VVT2-2] |
Genome annotation databases
EnsemblMetazoai | FBtr0075081; FBpp0074848; FBgn0036816 [Q9VVT2-1] FBtr0075082; FBpp0074849; FBgn0036816 [Q9VVT2-2] FBtr0075083; FBpp0074850; FBgn0036816 [Q9VVT2-2] FBtr0301857; FBpp0291071; FBgn0036816 [Q9VVT2-2] |
GeneIDi | 40049 |
KEGGi | dme:Dmel_CG3979 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF509505 mRNA Translation: AAN86815.1 AE014296 Genomic DNA Translation: AAF49226.1 AE014296 Genomic DNA Translation: AAF49227.2 AE014296 Genomic DNA Translation: AAN11670.1 AY102686 mRNA Translation: AAM27515.1 AF217399 mRNA Translation: AAF73384.1 Different initiation. |
RefSeqi | NP_001163465.1, NM_001169994.2 [Q9VVT2-2] NP_524150.1, NM_079426.4 [Q9VVT2-2] NP_730363.2, NM_168778.2 [Q9VVT2-1] NP_730364.1, NM_168779.2 [Q9VVT2-2] |
3D structure databases
AlphaFoldDBi | Q9VVT2 |
SMRi | Q9VVT2 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 65330, 4 interactors |
DIPi | DIP-18443N |
IntActi | Q9VVT2, 3 interactors |
STRINGi | 7227.FBpp0074848 |
Protein family/group databases
TCDBi | 2.A.47.1.10, the divalent anion:na(+) symporter (dass) family |
PTM databases
SwissPalmi | Q9VVT2 |
Proteomic databases
PaxDbi | Q9VVT2 |
PRIDEi | Q9VVT2 |
Protocols and materials databases
DNASUi | 40049 |
Genome annotation databases
EnsemblMetazoai | FBtr0075081; FBpp0074848; FBgn0036816 [Q9VVT2-1] FBtr0075082; FBpp0074849; FBgn0036816 [Q9VVT2-2] FBtr0075083; FBpp0074850; FBgn0036816 [Q9VVT2-2] FBtr0301857; FBpp0291071; FBgn0036816 [Q9VVT2-2] |
GeneIDi | 40049 |
KEGGi | dme:Dmel_CG3979 |
Organism-specific databases
CTDi | 40049 |
FlyBasei | FBgn0036816, Indy |
VEuPathDBi | VectorBase:FBgn0036816 |
Phylogenomic databases
eggNOGi | KOG1281, Eukaryota |
GeneTreei | ENSGT01030000234550 |
InParanoidi | Q9VVT2 |
PhylomeDBi | Q9VVT2 |
Enzyme and pathway databases
Reactomei | R-DME-433137, Sodium-coupled sulphate, di- and tri-carboxylate transporters |
SignaLinki | Q9VVT2 |
Miscellaneous databases
BioGRID-ORCSi | 40049, 0 hits in 3 CRISPR screens |
ChiTaRSi | Indy, fly |
GenomeRNAii | 40049 |
PROi | PR:Q9VVT2 |
Gene expression databases
Bgeei | FBgn0036816, Expressed in arthropod fat body and 46 other tissues |
ExpressionAtlasi | Q9VVT2, baseline and differential |
Genevisiblei | Q9VVT2, DM |
Family and domain databases
InterProi | View protein in InterPro IPR031312, Na/sul_symport_CS IPR001898, SLC13A/DASS |
Pfami | View protein in Pfam PF00939, Na_sulph_symp, 1 hit |
PROSITEi | View protein in PROSITE PS01271, NA_SULFATE, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | INDY1_DROME | |
Accessioni | Q9VVT2Primary (citable) accession number: Q9VVT2 Secondary accession number(s): A4V236, Q7KUS6, Q9NHY9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 13, 2004 |
Last sequence update: | November 13, 2007 | |
Last modified: | May 25, 2022 | |
This is version 155 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - SIMILARITY comments
Index of protein domains and families