UniProtKB - Q9VUQ5 (AGO2_DROME)
Protein argonaute-2
AGO2
Functioni
Essential for RNA interference (RNAi); double-stranded RNA induces potent and specific gene silencing (PubMed:11498593, PubMed:12368261, PubMed:14508492, PubMed:32504809).
RNAi is mediated by the RNA-induced silencing complex (RISC), a sequence-specific, multicomponent nuclease that destroys or silences messenger RNAs homologous to the silencing trigger (PubMed:11498593, PubMed:12368261, PubMed:14508492).
4 PublicationsCofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 965 | Divalent metal cationBy similarity | 1 | |
Metal bindingi | 1037 | Divalent metal cationBy similarity | 1 | |
Metal bindingi | 1173 | Divalent metal cationBy similarity | 1 |
GO - Molecular functioni
- endoribonuclease activity Source: FlyBase
- endoribonuclease activity, cleaving siRNA-paired mRNA Source: FlyBase
- metal ion binding Source: UniProtKB-KW
- miRNA binding Source: GO_Central
- RNA binding Source: GO_Central
- single-stranded RNA binding Source: FlyBase
- siRNA binding Source: FlyBase
GO - Biological processi
- cellularization Source: FlyBase
- cellular response to virus Source: FlyBase
- defense response to virus Source: FlyBase
- dosage compensation by hyperactivation of X chromosome Source: FlyBase
- dsRNA transport Source: FlyBase
- gene silencing by miRNA Source: FlyBase
- gene silencing by RNA-directed DNA methylation Source: FlyBase
- negative regulation of transposition, RNA-mediated Source: FlyBase
- negative regulation of viral genome replication Source: FlyBase
- pole cell formation Source: FlyBase
- production of siRNA involved in gene silencing by small RNA Source: FlyBase
- production of siRNA involved in RNA interference Source: UniProtKB
- RNAi-mediated antiviral immune response Source: FlyBase
- RNA interference Source: FlyBase
- segment polarity determination Source: FlyBase
- siRNA loading onto RISC involved in RNA interference Source: FlyBase
- small RNA loading onto RISC Source: FlyBase
- syncytial nuclear migration Source: FlyBase
Keywordsi
Molecular function | RNA-binding |
Biological process | Host-virus interaction, RNA-mediated gene silencing |
Ligand | Magnesium, Manganese, Metal-binding |
Enzyme and pathway databases
Reactomei | R-DME-203927, MicroRNA (miRNA) biogenesis R-DME-426486, Small interfering RNA (siRNA) biogenesis R-DME-426496, Post-transcriptional silencing by small RNAs R-DME-5578749, Transcriptional regulation by small RNAs |
SignaLinki | Q9VUQ5 |
Names & Taxonomyi
Protein namesi | Recommended name: Protein argonaute-2 |
Gene namesi | Name:AGO2Imported ORF Names:CG7439 |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0087035, AGO2 |
VEuPathDBi | VectorBase:FBgn0087035 |
Subcellular locationi
Other locations
- Cytoplasmic ribonucleoprotein granule 1 Publication
Cytosol
- cytosol Source: FlyBase
Nucleus
- nucleus Source: FlyBase
Other locations
- cytoplasm Source: FlyBase
- messenger ribonucleoprotein complex Source: UniProtKB
- neuronal ribonucleoprotein granule Source: UniProtKB
- RISC complex Source: UniProtKB
- RISC-loading complex Source: FlyBase
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 966 | V → M: Impaired conversion of the pre-RISC complex, containing duplex siRNA, to the holo-RISC complex, containing single-stranded guide siRNA. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000194071 | 1 – 1214 | Protein argonaute-2Add BLAST | 1214 |
Proteomic databases
PaxDbi | Q9VUQ5 |
PRIDEi | Q9VUQ5 |
Expressioni
Gene expression databases
Bgeei | FBgn0087035, Expressed in arthropod fat body and 23 other tissues |
ExpressionAtlasi | Q9VUQ5, baseline and differential |
Genevisiblei | Q9VUQ5, DM |
Interactioni
Subunit structurei
Interacts with Fmr1, Dcr-1 and vig to form the RNA-induced silencing complex (RISC), a ribonucleoprotein (RNP) complex involved in translation regulation, other components of the complex are RpL5, RpL11 and Rm62 (PubMed:11498593, PubMed:14508492, PubMed:12368261). As part of the RISC complex, interacts with Tudor-SN (PubMed:14508492).
3 Publications(Microbial infection) Interacts with cricket paralysis virus protein 1A; this interaction may block the RISC activity.
1 PublicationBinary interactionsi
Q9VUQ5
With | #Exp. | IntAct |
---|---|---|
Fmr1 [Q9NFU0] | 4 | EBI-442476,EBI-422631 |
ORF1 [Q9IJX4] from Cricket paralysis virus (isolate Teleogryllus commodus/Australia/CrPVVIC/1968). | 3 | EBI-442476,EBI-15848754 |
Protein-protein interaction databases
BioGRIDi | 65002, 70 interactors |
DIPi | DIP-41570N |
IntActi | Q9VUQ5, 32 interactors |
MINTi | Q9VUQ5 |
STRINGi | 7227.FBpp0075313 |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | Q9VUQ5 |
BMRBi | Q9VUQ5 |
SMRi | Q9VUQ5 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9VUQ5 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 608 – 717 | PAZPROSITE-ProRule annotationAdd BLAST | 110 | |
Domaini | 885 – 1186 | PiwiPROSITE-ProRule annotationAdd BLAST | 302 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 412 | DisorderedSequence analysisAdd BLAST | 412 | |
Regioni | 681 – 686 | Interaction with guide RNASequence analysis | 6 | |
Regioni | 1075 – 1076 | Interaction with guide RNASequence analysis | 2 | |
Regioni | 1119 – 1127 | Interaction with guide RNASequence analysis | 9 | |
Regioni | 1156 – 1178 | Interaction with guide RNASequence analysisAdd BLAST | 23 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 14 – 390 | Polar residuesSequence analysisAdd BLAST | 377 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG1041, Eukaryota |
HOGENOMi | CLU_004544_7_1_1 |
InParanoidi | Q9VUQ5 |
PhylomeDBi | Q9VUQ5 |
Family and domain databases
CDDi | cd04657, Piwi_ago-like, 1 hit |
Gene3Di | 3.30.420.10, 1 hit |
InterProi | View protein in InterPro IPR014811, ArgoL1 IPR032472, ArgoL2 IPR032473, Argonaute_Mid_dom IPR032474, Argonaute_N IPR003100, PAZ_dom IPR036085, PAZ_dom_sf IPR003165, Piwi IPR045246, Piwi_ago-like IPR012337, RNaseH-like_sf IPR036397, RNaseH_sf |
Pfami | View protein in Pfam PF08699, ArgoL1, 1 hit PF16488, ArgoL2, 1 hit PF16487, ArgoMid, 1 hit PF16486, ArgoN, 1 hit PF02170, PAZ, 1 hit PF02171, Piwi, 1 hit |
SMARTi | View protein in SMART SM01163, DUF1785, 1 hit SM00950, Piwi, 1 hit |
SUPFAMi | SSF101690, SSF101690, 1 hit SSF53098, SSF53098, 1 hit |
PROSITEi | View protein in PROSITE PS50821, PAZ, 1 hit PS50822, PIWI, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MGKKDKNKKG GQDSAAAPQP QQQQKQQQQR QQQPQQLQQP QQLQQPQQLQ
60 70 80 90 100
QPQQQQQQQP HQQQQQSSRQ QPSTSSGGSR ASGFQQGGQQ QKSQDAEGWT
110 120 130 140 150
AQKKQGKQQV QGWTKQGQQG GHQQGRQGQD GGYQQRPPGQ QQGGHQQGRQ
160 170 180 190 200
GQEGGYQQRP PGQQQGGHQQ GRQGQEGGYQ QRPSGQQQGG HQQGRQGQEG
210 220 230 240 250
GYQQRPPGQQ QGGHQQGRQG QEGGYQQRPS GQQQGGHQQG RQGQEGGYQQ
260 270 280 290 300
RPPGQQQGGH QQGRQGQEGG YQQRPPGQQQ GGHEQGRQGQ EGGYQQRPSG
310 320 330 340 350
QQQGGHQQGR QGQEGGYQQR PSGQQQGGHQ QGRQGQEGGY QQRPSGQQQG
360 370 380 390 400
GHQQGRQGQE GGYQQRPPGQ QPNQTQSQGQ YQSRGPPQQQ QAAPLPLPPQ
410 420 430 440 450
PAGSIKRGTI GKPGQVGINY LDLDLSKMPS VAYHYDVKIM PERPKKFYRQ
460 470 480 490 500
AFEQFRVDQL GGAVLAYDGK ASCYSVDKLP LNSQNPEVTV TDRNGRTLRY
510 520 530 540 550
TIEIKETGDS TIDLKSLTTY MNDRIFDKPM RAMQCVEVVL ASPCHNKAIR
560 570 580 590 600
VGRSFFKMSD PNNRHELDDG YEALVGLYQA FMLGDRPFLN VDISHKSFPI
610 620 630 640 650
SMPMIEYLER FSLKAKINNT TNLDYSRRFL EPFLRGINVV YTPPQSFQSA
660 670 680 690 700
PRVYRVNGLS RAPASSETFE HDGKKVTIAS YFHSRNYPLK FPQLHCLNVG
710 720 730 740 750
SSIKSILLPI ELCSIEEGQA LNRKDGATQV ANMIKYAATS TNVRKRKIMN
760 770 780 790 800
LLQYFQHNLD PTISRFGIRI ANDFIVVSTR VLSPPQVEYH SKRFTMVKNG
810 820 830 840 850
SWRMDGMKFL EPKPKAHKCA VLYCDPRSGR KMNYTQLNDF GNLIISQGKA
860 870 880 890 900
VNISLDSDVT YRPFTDDERS LDTIFADLKR SQHDLAIVII PQFRISYDTI
910 920 930 940 950
KQKAELQHGI LTQCIKQFTV ERKCNNQTIG NILLKINSKL NGINHKIKDD
960 970 980 990 1000
PRLPMMKNTM YIGADVTHPS PDQREIPSVV GVAASHDPYG ASYNMQYRLQ
1010 1020 1030 1040 1050
RGALEEIEDM FSITLEHLRV YKEYRNAYPD HIIYYRDGVS DGQFPKIKNE
1060 1070 1080 1090 1100
ELRCIKQACD KVGCKPKICC VIVVKRHHTR FFPSGDVTTS NKFNNVDPGT
1110 1120 1130 1140 1150
VVDRTIVHPN EMQFFMVSHQ AIQGTAKPTR YNVIENTGNL DIDLLQQLTY
1160 1170 1180 1190 1200
NLCHMFPRCN RSVSYPAPAY LAHLVAARGR VYLTGTNRFL DLKKEYAKRT
1210
IVPEFMKKNP MYFV
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketM9PFK7 | M9PFK7_DROME | Argonaute 2, isoform E | AGO2 AG02, ago, AGO 2, Ago-2, ago-2 | 787 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 201 | G → D in AAO39550 (Ref. 3) Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 714 | S → R in strain: 186. 1 Publication | 1 | |
Natural varianti | 740 | S → P in strain: 130. 1 Publication | 1 | |
Natural varianti | 783 | S → N in strain: 138, 140 and 196. 2 Publications | 1 | |
Natural varianti | 835 | T → A in strain: 128, 130, 138, 140, 141, 186, 187 and 196. 2 Publications | 1 | |
Natural varianti | 854 | S → T in strain: 128, 130, 138, 140, 141, 186, 187 and 196. 2 Publications | 1 | |
Natural varianti | 866 | D → E in strain: 128, 130, 141, 186 and 187. 1 Publication | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_050781 | 1 – 6 | MGKKDK → MHFPITTPE in isoform C. 1 Publication | 6 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE014296 Genomic DNA Translation: AAF49619.2 AE014296 Genomic DNA Translation: AAF49620.2 BT003546 mRNA Translation: AAO39550.1 BT099682 mRNA Translation: ACV44468.1 DQ228766 Genomic DNA Translation: ABB54719.1 DQ228767 Genomic DNA Translation: ABB54720.1 DQ228768 Genomic DNA Translation: ABB54721.1 DQ228769 Genomic DNA Translation: ABB54722.1 DQ228770 Genomic DNA Translation: ABB54723.1 DQ228771 Genomic DNA Translation: ABB54724.1 DQ228772 Genomic DNA Translation: ABB54725.1 DQ228773 Genomic DNA Translation: ABB54726.1 AY094751 mRNA Translation: AAM11104.1 Different initiation. |
RefSeqi | NP_648775.1, NM_140518.3 [Q9VUQ5-1] NP_730054.1, NM_168626.3 [Q9VUQ5-2] |
Genome annotation databases
EnsemblMetazoai | FBtr0075559; FBpp0075312; FBgn0087035 [Q9VUQ5-1] FBtr0075560; FBpp0075313; FBgn0087035 [Q9VUQ5-2] |
GeneIDi | 39683 |
KEGGi | dme:Dmel_CG7439 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE014296 Genomic DNA Translation: AAF49619.2 AE014296 Genomic DNA Translation: AAF49620.2 BT003546 mRNA Translation: AAO39550.1 BT099682 mRNA Translation: ACV44468.1 DQ228766 Genomic DNA Translation: ABB54719.1 DQ228767 Genomic DNA Translation: ABB54720.1 DQ228768 Genomic DNA Translation: ABB54721.1 DQ228769 Genomic DNA Translation: ABB54722.1 DQ228770 Genomic DNA Translation: ABB54723.1 DQ228771 Genomic DNA Translation: ABB54724.1 DQ228772 Genomic DNA Translation: ABB54725.1 DQ228773 Genomic DNA Translation: ABB54726.1 AY094751 mRNA Translation: AAM11104.1 Different initiation. |
RefSeqi | NP_648775.1, NM_140518.3 [Q9VUQ5-1] NP_730054.1, NM_168626.3 [Q9VUQ5-2] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1R6Z | X-ray | 2.80 | A/P/Z | 589-726 | [»] | |
1T2R | NMR | - | A | 602-720 | [»] | |
1T2S | NMR | - | A | 602-720 | [»] | |
1VYN | NMR | - | A | 602-740 | [»] | |
3MJ0 | X-ray | 2.31 | A | 601-723 | [»] | |
AlphaFoldDBi | Q9VUQ5 | |||||
BMRBi | Q9VUQ5 | |||||
SMRi | Q9VUQ5 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 65002, 70 interactors |
DIPi | DIP-41570N |
IntActi | Q9VUQ5, 32 interactors |
MINTi | Q9VUQ5 |
STRINGi | 7227.FBpp0075313 |
Proteomic databases
PaxDbi | Q9VUQ5 |
PRIDEi | Q9VUQ5 |
Protocols and materials databases
ABCDi | Q9VUQ5, 1 sequenced antibody |
Genome annotation databases
EnsemblMetazoai | FBtr0075559; FBpp0075312; FBgn0087035 [Q9VUQ5-1] FBtr0075560; FBpp0075313; FBgn0087035 [Q9VUQ5-2] |
GeneIDi | 39683 |
KEGGi | dme:Dmel_CG7439 |
Organism-specific databases
CTDi | 27161 |
FlyBasei | FBgn0087035, AGO2 |
VEuPathDBi | VectorBase:FBgn0087035 |
Phylogenomic databases
eggNOGi | KOG1041, Eukaryota |
HOGENOMi | CLU_004544_7_1_1 |
InParanoidi | Q9VUQ5 |
PhylomeDBi | Q9VUQ5 |
Enzyme and pathway databases
Reactomei | R-DME-203927, MicroRNA (miRNA) biogenesis R-DME-426486, Small interfering RNA (siRNA) biogenesis R-DME-426496, Post-transcriptional silencing by small RNAs R-DME-5578749, Transcriptional regulation by small RNAs |
SignaLinki | Q9VUQ5 |
Miscellaneous databases
BioGRID-ORCSi | 39683, 1 hit in 1 CRISPR screen |
EvolutionaryTracei | Q9VUQ5 |
GenomeRNAii | 39683 |
PROi | PR:Q9VUQ5 |
Gene expression databases
Bgeei | FBgn0087035, Expressed in arthropod fat body and 23 other tissues |
ExpressionAtlasi | Q9VUQ5, baseline and differential |
Genevisiblei | Q9VUQ5, DM |
Family and domain databases
CDDi | cd04657, Piwi_ago-like, 1 hit |
Gene3Di | 3.30.420.10, 1 hit |
InterProi | View protein in InterPro IPR014811, ArgoL1 IPR032472, ArgoL2 IPR032473, Argonaute_Mid_dom IPR032474, Argonaute_N IPR003100, PAZ_dom IPR036085, PAZ_dom_sf IPR003165, Piwi IPR045246, Piwi_ago-like IPR012337, RNaseH-like_sf IPR036397, RNaseH_sf |
Pfami | View protein in Pfam PF08699, ArgoL1, 1 hit PF16488, ArgoL2, 1 hit PF16487, ArgoMid, 1 hit PF16486, ArgoN, 1 hit PF02170, PAZ, 1 hit PF02171, Piwi, 1 hit |
SMARTi | View protein in SMART SM01163, DUF1785, 1 hit SM00950, Piwi, 1 hit |
SUPFAMi | SSF101690, SSF101690, 1 hit SSF53098, SSF53098, 1 hit |
PROSITEi | View protein in PROSITE PS50821, PAZ, 1 hit PS50822, PIWI, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | AGO2_DROME | |
Accessioni | Q9VUQ5Primary (citable) accession number: Q9VUQ5 Secondary accession number(s): C7LAD2 Q9VUQ6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 16, 2004 |
Last sequence update: | August 16, 2004 | |
Last modified: | May 25, 2022 | |
This is version 161 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families