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UniProtKB - Q9VTY6 (UBE2C_DROME)
Protein
Ubiquitin-conjugating enzyme E2 C
Gene
vih
Organism
Drosophila melanogaster (Fruit fly)
Status
Functioni
Catalyzes the covalent attachment of ubiquitin to other proteins. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by initiating polyubiquitin chains on APC/C substrates, leading to the degradation of APC/C substrates by the proteasome and promoting mitotic exit.
PROSITE-ProRule annotation2 PublicationsCatalytic activityi
- S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotationBy similarity EC:2.3.2.23
- S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E1 ubiquitin-activating enzyme]-L-cysteine + N(6)-monoubiquitinyl-[acceptor protein]-L-lysine.By similarity EC:2.3.2.24
: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotationView all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 116 | Glycyl thioester intermediatePROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- ubiquitin conjugating enzyme activity Source: FlyBase
GO - Biological processi
- anaphase-promoting complex-dependent catabolic process Source: FlyBase
- exit from mitosis Source: UniProtKB
- positive regulation of cellular protein catabolic process Source: FlyBase
- positive regulation of mitotic metaphase/anaphase transition Source: FlyBase
- protein polyubiquitination Source: GO_Central
- regulation of mitotic metaphase/anaphase transition Source: GO_Central
Keywordsi
Molecular function | Transferase |
Biological process | Ubl conjugation pathway |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-DME-141430, Inactivation of APC/C via direct inhibition of the APC/C complex R-DME-174048, APC/C:Cdc20 mediated degradation of Cyclin B R-DME-174084, Autodegradation of Cdh1 by Cdh1:APC/C R-DME-174154, APC/C:Cdc20 mediated degradation of Securin R-DME-174178, APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1 R-DME-174184, Cdc20:Phospho-APC/C mediated degradation of Cyclin A R-DME-176407, Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase R-DME-176408, Regulation of APC/C activators between G1/S and early anaphase R-DME-176409, APC/C:Cdc20 mediated degradation of mitotic proteins R-DME-176412, Phosphorylation of the APC/C R-DME-179409, APC-Cdc20 mediated degradation of Nek2A R-DME-2467813, Separation of Sister Chromatids R-DME-2559582, Senescence-Associated Secretory Phenotype (SASP) R-DME-69017, CDK-mediated phosphorylation and removal of Cdc6 R-DME-8866652, Synthesis of active ubiquitin: roles of E1 and E2 enzymes R-DME-983168, Antigen processing: Ubiquitination & Proteasome degradation |
UniPathwayi | UPA00143 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:vih Synonyms:Ubch10 ORF Names:CG10682 |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0264848, vih |
VEuPathDBi | VectorBase:FBgn0264848 |
Subcellular locationi
Cytoskeleton
- centrosome 1 Publication
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Note: Localizes in the cytoplasm of mitotic cells but also associates with centrosomes, and its own degradation is initiated at the metaphase-anaphase transition.
Cytoskeleton
- microtubule organizing center Source: UniProtKB-SubCell
Nucleus
- nucleus Source: FlyBase
Other locations
- cytoplasm Source: UniProtKB-SubCell
- ubiquitin ligase complex Source: FlyBase
Keywords - Cellular componenti
Cytoplasm, CytoskeletonPathology & Biotechi
Disruption phenotypei
Embryonic lethality. Both homozygous and hemizygous females show maternal effect lethality and produce syncytial embryos that fail to develop as a result of mitotic defects.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000390471 | 1 – 178 | Ubiquitin-conjugating enzyme E2 CAdd BLAST | 178 |
Post-translational modificationi
Ubiquitinated by the APC/C complex, leading to its degradation at the metaphase-anaphase transition.1 Publication
Keywords - PTMi
Ubl conjugationProteomic databases
PaxDbi | Q9VTY6 |
PRIDEi | Q9VTY6 |
PTM databases
SwissPalmi | Q9VTY6 |
Expressioni
Gene expression databases
Bgeei | FBgn0264848, Expressed in embryo and 21 other tissues |
Genevisiblei | Q9VTY6, DM |
Interactioni
Subunit structurei
Component of the APC/C complex.
Protein-protein interaction databases
BioGRIDi | 68841, 9 interactors |
IntActi | Q9VTY6, 1 interactor |
STRINGi | 7227.FBpp0075690 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 32 – 177 | UBC corePROSITE-ProRule annotationAdd BLAST | 146 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 28 | DisorderedSequence analysisAdd BLAST | 28 |
Sequence similaritiesi
Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation
Phylogenomic databases
eggNOGi | KOG0421, Eukaryota |
GeneTreei | ENSGT00930000150941 |
HOGENOMi | CLU_030988_9_3_1 |
InParanoidi | Q9VTY6 |
OMAi | HPNVDMS |
OrthoDBi | 1444506at2759 |
PhylomeDBi | Q9VTY6 |
Family and domain databases
CDDi | cd00195, UBCc, 1 hit |
Gene3Di | 3.10.110.10, 1 hit |
InterProi | View protein in InterPro IPR000608, UBQ-conjugat_E2 IPR023313, UBQ-conjugating_AS IPR016135, UBQ-conjugating_enzyme/RWD |
Pfami | View protein in Pfam PF00179, UQ_con, 1 hit |
SUPFAMi | SSF54495, SSF54495, 1 hit |
PROSITEi | View protein in PROSITE PS00183, UBC_1, 1 hit PS50127, UBC_2, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9VTY6-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAQNISPEQS GGAGGGGSKH SDDSMPVKDN HAVSKRLHKE LMNLMMANER
60 70 80 90 100
GISAFPDGEN IFKWVGTIAG PRNTVYSGQT YRLSLDFPNS YPYAAPVVKF
110 120 130 140 150
LTSCFHPNVD LQGAICLDIL KDKWSALYDV RTILLSIQSL LGEPNNESPL
160 170
NAQAAMMWND QKEYKKYLDA FYEKHKDT
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF410850 mRNA Translation: AAL02117.1 AE014296 Genomic DNA Translation: AAF49909.1 BT028772 mRNA Translation: ABI34153.1 |
RefSeqi | NP_648582.1, NM_140325.3 |
Genome annotation databases
EnsemblMetazoai | FBtr0075958; FBpp0075690; FBgn0264848 |
GeneIDi | 44118 |
KEGGi | dme:Dmel_CG10682 |
UCSCi | CG10682-RA, d. melanogaster |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF410850 mRNA Translation: AAL02117.1 AE014296 Genomic DNA Translation: AAF49909.1 BT028772 mRNA Translation: ABI34153.1 |
RefSeqi | NP_648582.1, NM_140325.3 |
3D structure databases
AlphaFoldDBi | Q9VTY6 |
SMRi | Q9VTY6 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 68841, 9 interactors |
IntActi | Q9VTY6, 1 interactor |
STRINGi | 7227.FBpp0075690 |
PTM databases
SwissPalmi | Q9VTY6 |
Proteomic databases
PaxDbi | Q9VTY6 |
PRIDEi | Q9VTY6 |
Protocols and materials databases
DNASUi | 44118 |
Genome annotation databases
EnsemblMetazoai | FBtr0075958; FBpp0075690; FBgn0264848 |
GeneIDi | 44118 |
KEGGi | dme:Dmel_CG10682 |
UCSCi | CG10682-RA, d. melanogaster |
Organism-specific databases
CTDi | 44118 |
FlyBasei | FBgn0264848, vih |
VEuPathDBi | VectorBase:FBgn0264848 |
Phylogenomic databases
eggNOGi | KOG0421, Eukaryota |
GeneTreei | ENSGT00930000150941 |
HOGENOMi | CLU_030988_9_3_1 |
InParanoidi | Q9VTY6 |
OMAi | HPNVDMS |
OrthoDBi | 1444506at2759 |
PhylomeDBi | Q9VTY6 |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
Reactomei | R-DME-141430, Inactivation of APC/C via direct inhibition of the APC/C complex R-DME-174048, APC/C:Cdc20 mediated degradation of Cyclin B R-DME-174084, Autodegradation of Cdh1 by Cdh1:APC/C R-DME-174154, APC/C:Cdc20 mediated degradation of Securin R-DME-174178, APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1 R-DME-174184, Cdc20:Phospho-APC/C mediated degradation of Cyclin A R-DME-176407, Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase R-DME-176408, Regulation of APC/C activators between G1/S and early anaphase R-DME-176409, APC/C:Cdc20 mediated degradation of mitotic proteins R-DME-176412, Phosphorylation of the APC/C R-DME-179409, APC-Cdc20 mediated degradation of Nek2A R-DME-2467813, Separation of Sister Chromatids R-DME-2559582, Senescence-Associated Secretory Phenotype (SASP) R-DME-69017, CDK-mediated phosphorylation and removal of Cdc6 R-DME-8866652, Synthesis of active ubiquitin: roles of E1 and E2 enzymes R-DME-983168, Antigen processing: Ubiquitination & Proteasome degradation |
Miscellaneous databases
BioGRID-ORCSi | 44118, 0 hits in 1 CRISPR screen |
GenomeRNAii | 44118 |
PROi | PR:Q9VTY6 |
Gene expression databases
Bgeei | FBgn0264848, Expressed in embryo and 21 other tissues |
Genevisiblei | Q9VTY6, DM |
Family and domain databases
CDDi | cd00195, UBCc, 1 hit |
Gene3Di | 3.10.110.10, 1 hit |
InterProi | View protein in InterPro IPR000608, UBQ-conjugat_E2 IPR023313, UBQ-conjugating_AS IPR016135, UBQ-conjugating_enzyme/RWD |
Pfami | View protein in Pfam PF00179, UQ_con, 1 hit |
SUPFAMi | SSF54495, SSF54495, 1 hit |
PROSITEi | View protein in PROSITE PS00183, UBC_1, 1 hit PS50127, UBC_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | UBE2C_DROME | |
Accessioni | Q9VTY6Primary (citable) accession number: Q9VTY6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 15, 2009 |
Last sequence update: | May 1, 2000 | |
Last modified: | May 25, 2022 | |
This is version 157 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families