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Protein

Pyrimidodiazepine synthase

Gene

se

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Mediates the conversion of 2-amino-4-oxo-6-pyruvoyl-5,6,7,8-tetrahydropteridine (6-PTP; also named 6-pyruvoyltetrahydropterin) to 2-amino-6-acetyl-3,7,8,9-tetrahydro-3H-pyrimido(4,5-b)[1,4]diazepin-4-one (pyrimidodiazepine or PDA), a key intermediate in red eye pigment drosopterin biosynthesis.2 Publications

Catalytic activityi

2-amino-6-acetyl-3,7,8,9-tetrahydro-3H-pyrimido(4,5-b)[1,4]diazepin-4-one + glutathione disulfide + H2O = 6-pyruvoyltetrahydropterin + 2 glutathione.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei30NucleophileBy similarity1
Binding sitei57GlutathioneBy similarity1
Binding sitei70Glutathione; via amide nitrogen and carbonyl oxygenBy similarity1

GO - Molecular functioni

GO - Biological processi

  • eye pigment biosynthetic process Source: FlyBase
  • glutathione metabolic process Source: FlyBase
  • oxidation-reduction process Source: GO_Central
  • pteridine biosynthetic process Source: FlyBase

Keywordsi

Molecular functionOxidoreductase

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-18458
ReactomeiR-DME-156581 Methylation
R-DME-156590 Glutathione conjugation
R-DME-196836 Vitamin C (ascorbate) metabolism

Names & Taxonomyi

Protein namesi
Recommended name:
Pyrimidodiazepine synthaseCurated (EC:1.5.4.11 Publication)
Alternative name(s):
Protein sepia1 Publication
Gene namesi
Name:seImported
ORF Names:CG6781Imported
OrganismiDrosophila melanogaster (Fruit fly)Imported
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0086348 se

Subcellular locationi

Pathology & Biotechi

Disruption phenotypei

Sepia eye color, due to defects in 2-amino-6-acetyl-3,7,8,9-tetrahydro-3H-pyrimido(4,5-b)[1,4]diazepin-4-one (pyrimidodiazepine or PDA) synthesis, a key intermediate in red eye pigment drosopterin biosynthesis.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004305821 – 243Pyrimidodiazepine synthaseAdd BLAST243

Proteomic databases

PaxDbiQ9VSL3
PRIDEiQ9VSL3

Expressioni

Developmental stagei

Appears only at the late pupal stage; expressed at high level. Also expressed in the early adult (0-2 days old) at lower level. Expressed exclusively in the adult head.1 Publication

Gene expression databases

BgeeiFBgn0086348 Expressed in 4 organ(s), highest expression level in head
GenevisibleiQ9VSL3 DM

Interactioni

Subunit structurei

Homodimer.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi64383, 1 interactor
IntActiQ9VSL3, 1 interactor
STRINGi7227.FBpp0076349

Structurei

3D structure databases

ProteinModelPortaliQ9VSL3
SMRiQ9VSL3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini20 – 102GST N-terminalSequence analysisAdd BLAST83
Domaini107 – 230GST C-terminalSequence analysisAdd BLAST124

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni86 – 87Glutathione bindingBy similarity2

Sequence similaritiesi

Belongs to the GST superfamily. Omega family.Curated

Phylogenomic databases

eggNOGiKOG0406 Eukaryota
ENOG410XSIX LUCA
GeneTreeiENSGT00390000005479
InParanoidiQ9VSL3
KOiK00310
OMAiEHPLKLY
OrthoDBiEOG091G0IEA
PhylomeDBiQ9VSL3

Family and domain databases

InterProiView protein in InterPro
IPR010987 Glutathione-S-Trfase_C-like
IPR036282 Glutathione-S-Trfase_C_sf
IPR040079 Glutathione_S-Trfase
IPR004045 Glutathione_S-Trfase_N
IPR005442 GST_omega
IPR036249 Thioredoxin-like_sf
PfamiView protein in Pfam
PF13417 GST_N_3, 1 hit
PRINTSiPR01625 GSTRNSFRASEO
SFLDiSFLDS00019 Glutathione_Transferase_(cytos, 1 hit
SUPFAMiSSF47616 SSF47616, 1 hit
SSF52833 SSF52833, 1 hit
PROSITEiView protein in PROSITE
PS50405 GST_CTER, 1 hit
PS50404 GST_NTER, 1 hit

Sequencei

Sequence statusi: Complete.

Q9VSL3-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSNGRHLAKG SPMPDVPEDG ILRLYSMRFC PFAQRVHLVL DAKQIPYHSI
60 70 80 90 100
YINLTDKPEW LLEKNPQGKV PALEIVREPG PPVLTESLLI CEYLDEQYPL
110 120 130 140 150
RPLYPRDPLK KVQDKLLIER FRAVLGAFFK ASDGGDLEPF WSGLDIYERE
160 170 180 190 200
LARRGTEFFG GEQTGILDYM IWPWCERLEL LKLQRGEDYN YDQSRFPQLT
210 220 230 240
LWLERMKRDP AVMAFYMEAE VQAEFLRTRS LGRPNYNLLV KDA
Length:243
Mass (Da):28,413
Last modified:May 1, 2000 - v1
Checksum:iA6B651F36487A5AE
GO

Sequence cautioni

The sequence ACZ02425 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence ACZ02430 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014296 Genomic DNA Translation: AAF50405.1
AY070667 mRNA Translation: AAL48138.1
GQ351317 Genomic DNA Translation: ACZ02425.1 Sequence problems.
GQ351318 Genomic DNA Translation: ACZ02430.1 Sequence problems.
RefSeqiNP_648235.1, NM_139978.4
UniGeneiDm.6327

Genome annotation databases

EnsemblMetazoaiFBtr0076623; FBpp0076349; FBgn0086348
GeneIDi38973
KEGGidme:Dmel_CG6781
UCSCiCG6781-RA d. melanogaster

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014296 Genomic DNA Translation: AAF50405.1
AY070667 mRNA Translation: AAL48138.1
GQ351317 Genomic DNA Translation: ACZ02425.1 Sequence problems.
GQ351318 Genomic DNA Translation: ACZ02430.1 Sequence problems.
RefSeqiNP_648235.1, NM_139978.4
UniGeneiDm.6327

3D structure databases

ProteinModelPortaliQ9VSL3
SMRiQ9VSL3
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi64383, 1 interactor
IntActiQ9VSL3, 1 interactor
STRINGi7227.FBpp0076349

Proteomic databases

PaxDbiQ9VSL3
PRIDEiQ9VSL3

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0076623; FBpp0076349; FBgn0086348
GeneIDi38973
KEGGidme:Dmel_CG6781
UCSCiCG6781-RA d. melanogaster

Organism-specific databases

CTDi38973
FlyBaseiFBgn0086348 se

Phylogenomic databases

eggNOGiKOG0406 Eukaryota
ENOG410XSIX LUCA
GeneTreeiENSGT00390000005479
InParanoidiQ9VSL3
KOiK00310
OMAiEHPLKLY
OrthoDBiEOG091G0IEA
PhylomeDBiQ9VSL3

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-18458
ReactomeiR-DME-156581 Methylation
R-DME-156590 Glutathione conjugation
R-DME-196836 Vitamin C (ascorbate) metabolism

Miscellaneous databases

GenomeRNAii38973
PROiPR:Q9VSL3

Gene expression databases

BgeeiFBgn0086348 Expressed in 4 organ(s), highest expression level in head
GenevisibleiQ9VSL3 DM

Family and domain databases

InterProiView protein in InterPro
IPR010987 Glutathione-S-Trfase_C-like
IPR036282 Glutathione-S-Trfase_C_sf
IPR040079 Glutathione_S-Trfase
IPR004045 Glutathione_S-Trfase_N
IPR005442 GST_omega
IPR036249 Thioredoxin-like_sf
PfamiView protein in Pfam
PF13417 GST_N_3, 1 hit
PRINTSiPR01625 GSTRNSFRASEO
SFLDiSFLDS00019 Glutathione_Transferase_(cytos, 1 hit
SUPFAMiSSF47616 SSF47616, 1 hit
SSF52833 SSF52833, 1 hit
PROSITEiView protein in PROSITE
PS50405 GST_CTER, 1 hit
PS50404 GST_NTER, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSEPIA_DROME
AccessioniPrimary (citable) accession number: Q9VSL3
Secondary accession number(s): D1KS67, D1KS72
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 29, 2014
Last sequence update: May 1, 2000
Last modified: November 7, 2018
This is version 164 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
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Main funding by: National Institutes of Health

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