UniProtKB - Q9VSL3 (SEPIA_DROME)
Protein
Pyrimidodiazepine synthase
Gene
se
Organism
Drosophila melanogaster (Fruit fly)
Status
Functioni
Mediates the conversion of 2-amino-4-oxo-6-pyruvoyl-5,6,7,8-tetrahydropteridine (6-PTP; also named 6-pyruvoyltetrahydropterin) to 2-amino-6-acetyl-3,7,8,9-tetrahydro-3H-pyrimido(4,5-b)[1,4]diazepin-4-one (pyrimidodiazepine or PDA), a key intermediate in red eye pigment drosopterin biosynthesis.2 Publications
Catalytic activityi
- 2-amino-6-acetyl-3,7,8,9-tetrahydro-3H-pyrimido[4,5-b][1,4]diazepin-4-one + glutathione disulfide + H2O = 6-pyruvoyl-5,6,7,8-tetrahydropterin + 2 glutathione1 PublicationEC:1.5.4.11 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 30 | NucleophileBy similarity | 1 | |
Binding sitei | 57 | GlutathioneBy similarity | 1 | |
Binding sitei | 70 | Glutathione; via amide nitrogen and carbonyl oxygenBy similarity | 1 |
GO - Molecular functioni
- glutathione dehydrogenase (ascorbate) activity Source: FlyBase
- glutathione transferase activity Source: FlyBase
- protein homodimerization activity Source: FlyBase
- pyrimidodiazepine synthase activity Source: FlyBase
- transferase activity, transferring sulfur-containing groups Source: FlyBase
GO - Biological processi
- eye pigment biosynthetic process Source: FlyBase
- glutathione metabolic process Source: FlyBase
- oxidation-reduction process Source: GO_Central
- pteridine biosynthetic process Source: FlyBase
Keywordsi
Molecular function | Oxidoreductase |
Enzyme and pathway databases
BioCyci | MetaCyc:MONOMER-18458 |
Reactomei | R-DME-156581, Methylation R-DME-156590, Glutathione conjugation R-DME-196836, Vitamin C (ascorbate) metabolism |
Names & Taxonomyi
Protein namesi | Recommended name: Pyrimidodiazepine synthaseCurated (EC:1.5.4.11 Publication)Alternative name(s): Protein sepia1 Publication |
Gene namesi | Name:seImported ORF Names:CG6781Imported |
Organismi | Drosophila melanogaster (Fruit fly)Imported |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Holometabola › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0086348, se |
Subcellular locationi
Other locations
- cytoplasm Source: FlyBase
Pathology & Biotechi
Disruption phenotypei
Sepia eye color, due to defects in 2-amino-6-acetyl-3,7,8,9-tetrahydro-3H-pyrimido(4,5-b)[1,4]diazepin-4-one (pyrimidodiazepine or PDA) synthesis, a key intermediate in red eye pigment drosopterin biosynthesis.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000430582 | 1 – 243 | Pyrimidodiazepine synthaseAdd BLAST | 243 |
Proteomic databases
PaxDbi | Q9VSL3 |
PRIDEi | Q9VSL3 |
Expressioni
Developmental stagei
Appears only at the late pupal stage; expressed at high level. Also expressed in the early adult (0-2 days old) at lower level. Expressed exclusively in the adult head.1 Publication
Gene expression databases
Bgeei | FBgn0086348, Expressed in head and 17 other tissues |
Genevisiblei | Q9VSL3, DM |
Interactioni
Subunit structurei
Homodimer.
1 PublicationGO - Molecular functioni
- protein homodimerization activity Source: FlyBase
Protein-protein interaction databases
BioGRIDi | 64383, 1 interactor |
IntActi | Q9VSL3, 1 interactor |
STRINGi | 7227.FBpp0076349 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 20 – 102 | GST N-terminalSequence analysisAdd BLAST | 83 | |
Domaini | 107 – 230 | GST C-terminalSequence analysisAdd BLAST | 124 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 86 – 87 | Glutathione bindingBy similarity | 2 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0406, Eukaryota |
GeneTreei | ENSGT00940000163896 |
HOGENOMi | CLU_011226_9_2_1 |
InParanoidi | Q9VSL3 |
OMAi | EHPLKLY |
OrthoDBi | 1225872at2759 |
PhylomeDBi | Q9VSL3 |
Family and domain databases
Gene3Di | 3.40.30.10, 1 hit |
InterProi | View protein in InterPro IPR010987, Glutathione-S-Trfase_C-like IPR036282, Glutathione-S-Trfase_C_sf IPR040079, Glutathione_S-Trfase IPR004045, Glutathione_S-Trfase_N IPR005442, GST_omega IPR036249, Thioredoxin-like_sf |
Pfami | View protein in Pfam PF13417, GST_N_3, 1 hit |
PRINTSi | PR01625, GSTRNSFRASEO |
SFLDi | SFLDS00019, Glutathione_Transferase_(cytos, 1 hit |
SUPFAMi | SSF47616, SSF47616, 1 hit SSF52833, SSF52833, 1 hit |
PROSITEi | View protein in PROSITE PS50405, GST_CTER, 1 hit PS50404, GST_NTER, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9VSL3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSNGRHLAKG SPMPDVPEDG ILRLYSMRFC PFAQRVHLVL DAKQIPYHSI
60 70 80 90 100
YINLTDKPEW LLEKNPQGKV PALEIVREPG PPVLTESLLI CEYLDEQYPL
110 120 130 140 150
RPLYPRDPLK KVQDKLLIER FRAVLGAFFK ASDGGDLEPF WSGLDIYERE
160 170 180 190 200
LARRGTEFFG GEQTGILDYM IWPWCERLEL LKLQRGEDYN YDQSRFPQLT
210 220 230 240
LWLERMKRDP AVMAFYMEAE VQAEFLRTRS LGRPNYNLLV KDA
Sequence cautioni
The sequence ACZ02425 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence ACZ02430 differs from that shown. Reason: Erroneous gene model prediction.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE014296 Genomic DNA Translation: AAF50405.1 AY070667 mRNA Translation: AAL48138.1 GQ351317 Genomic DNA Translation: ACZ02425.1 Sequence problems. GQ351318 Genomic DNA Translation: ACZ02430.1 Sequence problems. |
RefSeqi | NP_648235.1, NM_139978.4 |
Genome annotation databases
EnsemblMetazoai | FBtr0076623; FBpp0076349; FBgn0086348 |
GeneIDi | 38973 |
KEGGi | dme:Dmel_CG6781 |
UCSCi | CG6781-RA, d. melanogaster |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE014296 Genomic DNA Translation: AAF50405.1 AY070667 mRNA Translation: AAL48138.1 GQ351317 Genomic DNA Translation: ACZ02425.1 Sequence problems. GQ351318 Genomic DNA Translation: ACZ02430.1 Sequence problems. |
RefSeqi | NP_648235.1, NM_139978.4 |
3D structure databases
SMRi | Q9VSL3 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 64383, 1 interactor |
IntActi | Q9VSL3, 1 interactor |
STRINGi | 7227.FBpp0076349 |
Proteomic databases
PaxDbi | Q9VSL3 |
PRIDEi | Q9VSL3 |
Genome annotation databases
EnsemblMetazoai | FBtr0076623; FBpp0076349; FBgn0086348 |
GeneIDi | 38973 |
KEGGi | dme:Dmel_CG6781 |
UCSCi | CG6781-RA, d. melanogaster |
Organism-specific databases
CTDi | 38973 |
FlyBasei | FBgn0086348, se |
Phylogenomic databases
eggNOGi | KOG0406, Eukaryota |
GeneTreei | ENSGT00940000163896 |
HOGENOMi | CLU_011226_9_2_1 |
InParanoidi | Q9VSL3 |
OMAi | EHPLKLY |
OrthoDBi | 1225872at2759 |
PhylomeDBi | Q9VSL3 |
Enzyme and pathway databases
BioCyci | MetaCyc:MONOMER-18458 |
Reactomei | R-DME-156581, Methylation R-DME-156590, Glutathione conjugation R-DME-196836, Vitamin C (ascorbate) metabolism |
Miscellaneous databases
BioGRID-ORCSi | 38973, 0 hits in 3 CRISPR screens |
GenomeRNAii | 38973 |
PROi | PR:Q9VSL3 |
Gene expression databases
Bgeei | FBgn0086348, Expressed in head and 17 other tissues |
Genevisiblei | Q9VSL3, DM |
Family and domain databases
Gene3Di | 3.40.30.10, 1 hit |
InterProi | View protein in InterPro IPR010987, Glutathione-S-Trfase_C-like IPR036282, Glutathione-S-Trfase_C_sf IPR040079, Glutathione_S-Trfase IPR004045, Glutathione_S-Trfase_N IPR005442, GST_omega IPR036249, Thioredoxin-like_sf |
Pfami | View protein in Pfam PF13417, GST_N_3, 1 hit |
PRINTSi | PR01625, GSTRNSFRASEO |
SFLDi | SFLDS00019, Glutathione_Transferase_(cytos, 1 hit |
SUPFAMi | SSF47616, SSF47616, 1 hit SSF52833, SSF52833, 1 hit |
PROSITEi | View protein in PROSITE PS50405, GST_CTER, 1 hit PS50404, GST_NTER, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SEPIA_DROME | |
Accessioni | Q9VSL3Primary (citable) accession number: Q9VSL3 Secondary accession number(s): D1KS67, D1KS72 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 29, 2014 |
Last sequence update: | May 1, 2000 | |
Last modified: | February 10, 2021 | |
This is version 177 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - SIMILARITY comments
Index of protein domains and families