UniProtKB - Q9VQB7 (EOGT_DROME)
EGF domain-specific O-linked N-acetylglucosamine transferase
Eogt
Functioni
Catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to a serine or threonine residue in extracellular proteins resulting in their modification with a beta-linked N-acetylglucosamine (O-GlcNAc). Specifically glycosylates the Thr residue located between the fifth and sixth conserved cysteines of folded EGF-like domains. Involved in epithelial cell adhesion/interaction with the extracellular matrix by mediating glycosylation of proteins in the secretory pathway, such as Dumpy (Dp).
1 PublicationCatalytic activityi
- L-seryl-[protein] + UDP-N-acetyl-α-D-glucosamine = 3-O-(N-acetyl-β-D-glucosaminyl)-L-seryl-[protein] + H+ + UDP1 PublicationEC:2.4.1.2551 Publication
- L-threonyl-[protein] + UDP-N-acetyl-α-D-glucosamine = 3-O-(N-acetyl-β-D-glucosaminyl)-L-threonyl-[protein] + H+ + UDP1 PublicationEC:2.4.1.2551 Publication
Cofactori
GO - Molecular functioni
- glycosyltransferase activity Source: GO_Central
- protein N-acetylglucosaminyltransferase activity Source: UniProtKB
- protein O-GlcNAc transferase activity Source: FlyBase
GO - Biological processi
- larval chitin-based cuticle development Source: FlyBase
- protein O-GlcNAcylation via threonine Source: FlyBase
- protein O-linked glycosylation Source: UniProtKB
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Enzyme and pathway databases
BRENDAi | 2.4.1.255, 1994 |
SignaLinki | Q9VQB7 |
Protein family/group databases
CAZyi | GT61, Glycosyltransferase Family 61 |
Names & Taxonomyi
Protein namesi | Recommended name: EGF domain-specific O-linked N-acetylglucosamine transferase (EC:2.4.1.2551 Publication)Alternative name(s): Extracellular O-linked N-acetylglucosamine transferase |
Gene namesi | Name:Eogt ORF Names:CG9867 |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0264672, Eogt |
VEuPathDBi | VectorBase:FBgn0264672 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum lumen PROSITE-ProRule annotation1 Publication
Endoplasmic reticulum
- endoplasmic reticulum lumen Source: UniProtKB
Keywords - Cellular componenti
Endoplasmic reticulumPathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 16 | Sequence analysisAdd BLAST | 16 | |
ChainiPRO_0000418382 | 17 – 520 | EGF domain-specific O-linked N-acetylglucosamine transferaseAdd BLAST | 504 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 52 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 176 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 250 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 479 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
GlycoproteinProteomic databases
PaxDbi | Q9VQB7 |
PTM databases
GlyGeni | Q9VQB7, 4 sites |
Expressioni
Developmental stagei
Gene expression databases
Bgeei | FBgn0264672, Expressed in wing disc (Drosophila) and 40 other tissues |
ExpressionAtlasi | Q9VQB7, baseline and differential |
Genevisiblei | Q9VQB7, DM |
Interactioni
Protein-protein interaction databases
BioGRIDi | 59652, 3 interactors |
IntActi | Q9VQB7, 1 interactor |
STRINGi | 7227.FBpp0077447 |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 292 – 294 | Required for optimal activityBy similarity | 3 | |
Motifi | 517 – 520 | Prevents secretion from ERPROSITE-ProRule annotation | 4 |
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG4698, Eukaryota |
GeneTreei | ENSGT00940000156493 |
HOGENOMi | CLU_039300_0_0_1 |
InParanoidi | Q9VQB7 |
OMAi | RFQTPQC |
OrthoDBi | 567582at2759 |
PhylomeDBi | Q9VQB7 |
Family and domain databases
InterProi | View protein in InterPro IPR007657, Glycosyltransferase_61 |
PANTHERi | PTHR20961, PTHR20961, 1 hit |
Pfami | View protein in Pfam PF04577, Glyco_transf_61, 1 hit |
PROSITEi | View protein in PROSITE PS00014, ER_TARGET, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MPILPILIGI LHLSLAEDAK HLDGFSLPSL PSEHLIRYLN TFPKLKQQLP
60 70 80 90 100
TNLTGKGTIS SACWGHERDC TPAGRFQTPQ CPGEHTGWAR SKEAQVRTFY
110 120 130 140 150
NQADFGYIQE QLSQLTPQCV PTYLGDSSLE CTHYLRFCRG RNLLFDFRGL
160 170 180 190 200
EQREERIRYH MDVLGPGQLL GHCKLNRTRL SGEMEHIGSA LQSWGPELRN
210 220 230 240 250
FDVLPHPVLE SGLCDVVVNT PTFIMKIDAT YNMYHHFCDF FNLYASLFVN
260 270 280 290 300
QSHPAAFNTD VQILIWETYP YDSPFRDTFK AFSQRPVWTL SDVEGKRVCF
310 320 330 340 350
KNVVLPLLPR MIFGLFYNTP IIQGCSNSGL FRAFSEFILH RLQIPYKPPQ
360 370 380 390 400
QKIRITYLSR RTKYRQVLNE DELLAPLEAN DKYDVQRVSY ERLPFTNQLA
410 420 430 440 450
ITRNTDILIG MHGAGLTHLL FLPNWACIFE LYNCEDPNCY KDLARLRGVR
460 470 480 490 500
YRTWEQRDLV YPQDEGHHPE GGAHAKFTNY SFDVKEFVHL VDGAAEEILS
510 520
HKEFPRRASE NPSKTQRNEL
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketM9PBV8 | M9PBV8_DROME | EGF-domain O-GlcNAc transferase, is... | Eogt Dmel\CG9867, eogt, CG9867, Dmel_CG9867 | 520 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB675601 mRNA Translation: BAL41443.1 AE014134 Genomic DNA Translation: AAF51259.1 AY058292 mRNA Translation: AAL13521.1 |
RefSeqi | NP_001259934.1, NM_001273005.1 NP_608678.1, NM_134834.3 |
Genome annotation databases
EnsemblMetazoai | FBtr0077767; FBpp0077447; FBgn0264672 FBtr0330685; FBpp0303533; FBgn0264672 |
GeneIDi | 33424 |
KEGGi | dme:Dmel_CG9867 |
UCSCi | CG9867-RA, d. melanogaster |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB675601 mRNA Translation: BAL41443.1 AE014134 Genomic DNA Translation: AAF51259.1 AY058292 mRNA Translation: AAL13521.1 |
RefSeqi | NP_001259934.1, NM_001273005.1 NP_608678.1, NM_134834.3 |
3D structure databases
AlphaFoldDBi | Q9VQB7 |
SMRi | Q9VQB7 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 59652, 3 interactors |
IntActi | Q9VQB7, 1 interactor |
STRINGi | 7227.FBpp0077447 |
Protein family/group databases
CAZyi | GT61, Glycosyltransferase Family 61 |
PTM databases
GlyGeni | Q9VQB7, 4 sites |
Proteomic databases
PaxDbi | Q9VQB7 |
Protocols and materials databases
DNASUi | 33424 |
Genome annotation databases
EnsemblMetazoai | FBtr0077767; FBpp0077447; FBgn0264672 FBtr0330685; FBpp0303533; FBgn0264672 |
GeneIDi | 33424 |
KEGGi | dme:Dmel_CG9867 |
UCSCi | CG9867-RA, d. melanogaster |
Organism-specific databases
CTDi | 285203 |
FlyBasei | FBgn0264672, Eogt |
VEuPathDBi | VectorBase:FBgn0264672 |
Phylogenomic databases
eggNOGi | KOG4698, Eukaryota |
GeneTreei | ENSGT00940000156493 |
HOGENOMi | CLU_039300_0_0_1 |
InParanoidi | Q9VQB7 |
OMAi | RFQTPQC |
OrthoDBi | 567582at2759 |
PhylomeDBi | Q9VQB7 |
Enzyme and pathway databases
BRENDAi | 2.4.1.255, 1994 |
SignaLinki | Q9VQB7 |
Miscellaneous databases
BioGRID-ORCSi | 33424, 0 hits in 1 CRISPR screen |
GenomeRNAii | 33424 |
PROi | PR:Q9VQB7 |
Gene expression databases
Bgeei | FBgn0264672, Expressed in wing disc (Drosophila) and 40 other tissues |
ExpressionAtlasi | Q9VQB7, baseline and differential |
Genevisiblei | Q9VQB7, DM |
Family and domain databases
InterProi | View protein in InterPro IPR007657, Glycosyltransferase_61 |
PANTHERi | PTHR20961, PTHR20961, 1 hit |
Pfami | View protein in Pfam PF04577, Glyco_transf_61, 1 hit |
PROSITEi | View protein in PROSITE PS00014, ER_TARGET, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | EOGT_DROME | |
Accessioni | Q9VQB7Primary (citable) accession number: Q9VQB7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 11, 2012 |
Last sequence update: | May 1, 2000 | |
Last modified: | May 25, 2022 | |
This is version 133 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase