UniProtKB - Q9VPQ6 (USH_DROME)
Zinc finger protein ush
ush
Functioni
Transcription regulator that modulates expression mediated by transcription factors of the GATA family such as pnr and srp. Represses transcription of proneural achaete-scute complex (AS-C), which is usually activated by pnr. Involved in cardiogenesis, blood, and eye development. During hematopoiesis, it is required to restrict the number of crystal cells, probably via its interaction with the isoform SrpNC of srp. Negatively regulates expression of sr. Probably acts by interacting with the GATA-type zinc finger of proteins such as pnr and srp, possibly antagonizing the interaction between the GATA-type zinc finger and some cofactor.
6 PublicationsSites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 210 | Zinc 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 213 | Zinc 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 226 | Zinc 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 231 | Zinc 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 343 | Zinc 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 346 | Zinc 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 359 | Zinc 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 364 | Zinc 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 728 | Zinc 3PROSITE-ProRule annotation | 1 | |
Metal bindingi | 731 | Zinc 3PROSITE-ProRule annotation | 1 | |
Metal bindingi | 744 | Zinc 3PROSITE-ProRule annotation | 1 | |
Metal bindingi | 749 | Zinc 3PROSITE-ProRule annotation | 1 | |
Metal bindingi | 799 | Zinc 4PROSITE-ProRule annotation | 1 | |
Metal bindingi | 802 | Zinc 4PROSITE-ProRule annotation | 1 | |
Metal bindingi | 815 | Zinc 4PROSITE-ProRule annotation | 1 | |
Metal bindingi | 820 | Zinc 4PROSITE-ProRule annotation | 1 | |
Metal bindingi | 1121 | Zinc 5PROSITE-ProRule annotation | 1 | |
Metal bindingi | 1124 | Zinc 5PROSITE-ProRule annotation | 1 | |
Metal bindingi | 1137 | Zinc 5PROSITE-ProRule annotation | 1 | |
Metal bindingi | 1142 | Zinc 5PROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 202 – 235 | CCHC FOG-type 1PROSITE-ProRule annotationAdd BLAST | 34 | |
Zinc fingeri | 279 – 301 | C2H2-type 1PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 335 – 368 | CCHC FOG-type 2PROSITE-ProRule annotationAdd BLAST | 34 | |
Zinc fingeri | 720 – 753 | CCHC FOG-type 3PROSITE-ProRule annotationAdd BLAST | 34 | |
Zinc fingeri | 791 – 824 | CCHC FOG-type 4PROSITE-ProRule annotationAdd BLAST | 34 | |
Zinc fingeri | 882 – 907 | C2H2-type 2PROSITE-ProRule annotationAdd BLAST | 26 | |
Zinc fingeri | 910 – 932 | C2H2-type 3PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 983 – 1006 | C2H2-type 4PROSITE-ProRule annotationAdd BLAST | 24 | |
Zinc fingeri | 1113 – 1146 | CCHC FOG-type 5PROSITE-ProRule annotationAdd BLAST | 34 |
GO - Molecular functioni
- DNA binding Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
- RNA polymerase II-specific DNA-binding transcription factor binding Source: FlyBase
GO - Biological processi
- amnioserosa maintenance Source: FlyBase
- cell differentiation Source: GO_Central
- chaeta development Source: FlyBase
- compound eye development Source: FlyBase
- germ-band shortening Source: FlyBase
- heart development Source: FlyBase
- larval lymph gland hemopoiesis Source: FlyBase
- lymph gland crystal cell differentiation Source: FlyBase
- lymph gland development Source: FlyBase
- lymph gland plasmatocyte differentiation Source: FlyBase
- negative regulation of DNA-binding transcription factor activity Source: FlyBase
- negative regulation of hemocyte differentiation Source: FlyBase
- negative regulation of transcription by RNA polymerase II Source: FlyBase
- positive regulation of antibacterial peptide biosynthetic process Source: FlyBase
- positive regulation of transcription by RNA polymerase II Source: GO_Central
- regulation of transcription, DNA-templated Source: FlyBase
Keywordsi
Molecular function | DNA-binding, Repressor |
Biological process | Transcription, Transcription regulation |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-DME-8936459, RUNX1 regulates genes involved in megakaryocyte differentiation and platelet function R-DME-983231, Factors involved in megakaryocyte development and platelet production |
SignaLinki | Q9VPQ6 |
Names & Taxonomyi
Protein namesi | Recommended name: Zinc finger protein ushAlternative name(s): Protein U-shaped |
Gene namesi | Name:ush ORF Names:CG2762 |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0003963, ush |
VEuPathDBi | VectorBase:FBgn0003963 |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
Nucleus
- nucleus Source: UniProtKB
Keywords - Cellular componenti
NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 231 | C → A: Abolishes interaction with GATA-type zinc fingers. 1 Publication | 1 | |
Mutagenesisi | 1142 | C → H: Transforms the C2HC-type zinc finger into a C2H2-type, leading to abolish interaction with pnr. | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000221047 | 1 – 1191 | Zinc finger protein ushAdd BLAST | 1191 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 116 | Phosphoserine1 Publication | 1 | |
Modified residuei | 118 | Phosphoserine1 Publication | 1 | |
Modified residuei | 1013 | Phosphoserine1 Publication | 1 | |
Modified residuei | 1015 | Phosphoserine1 Publication | 1 | |
Modified residuei | 1017 | Phosphoserine1 Publication | 1 | |
Modified residuei | 1156 | Phosphoserine1 Publication | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | Q9VPQ6 |
PTM databases
iPTMneti | Q9VPQ6 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | FBgn0003963, Expressed in spermathecum and 61 other tissues |
ExpressionAtlasi | Q9VPQ6, baseline and differential |
Genevisiblei | Q9VPQ6, DM |
Interactioni
Subunit structurei
Interacts with pnr, although weak this interaction is essential.
Interacts with the isoform SrpNC of srp.
Interacts with CtBP corepressor.
2 PublicationsBinary interactionsi
Q9VPQ6
With | #Exp. | IntAct |
---|---|---|
Gata1 [P17679] from Mus musculus. | 2 | EBI-110692,EBI-3903251 |
GO - Molecular functioni
Protein-protein interaction databases
BioGRIDi | 59481, 14 interactors |
IntActi | Q9VPQ6, 1 interactor |
STRINGi | 7227.FBpp0305228 |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | Q9VPQ6 |
BMRBi | Q9VPQ6 |
SMRi | Q9VPQ6 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9VPQ6 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 153 | DisorderedSequence analysisAdd BLAST | 153 | |
Regioni | 169 – 194 | DisorderedSequence analysisAdd BLAST | 26 | |
Regioni | 239 – 274 | DisorderedSequence analysisAdd BLAST | 36 | |
Regioni | 304 – 338 | DisorderedSequence analysisAdd BLAST | 35 | |
Regioni | 361 – 413 | DisorderedSequence analysisAdd BLAST | 53 | |
Regioni | 504 – 540 | DisorderedSequence analysisAdd BLAST | 37 | |
Regioni | 601 – 635 | DisorderedSequence analysisAdd BLAST | 35 | |
Regioni | 1011 – 1073 | DisorderedSequence analysisAdd BLAST | 63 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 13 – 30 | Basic and acidic residuesSequence analysisAdd BLAST | 18 | |
Compositional biasi | 110 – 151 | Pro residuesSequence analysisAdd BLAST | 42 | |
Compositional biasi | 522 – 536 | Basic and acidic residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 1013 – 1044 | Polar residuesSequence analysisAdd BLAST | 32 |
Domaini
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 202 – 235 | CCHC FOG-type 1PROSITE-ProRule annotationAdd BLAST | 34 | |
Zinc fingeri | 279 – 301 | C2H2-type 1PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 335 – 368 | CCHC FOG-type 2PROSITE-ProRule annotationAdd BLAST | 34 | |
Zinc fingeri | 720 – 753 | CCHC FOG-type 3PROSITE-ProRule annotationAdd BLAST | 34 | |
Zinc fingeri | 791 – 824 | CCHC FOG-type 4PROSITE-ProRule annotationAdd BLAST | 34 | |
Zinc fingeri | 882 – 907 | C2H2-type 2PROSITE-ProRule annotationAdd BLAST | 26 | |
Zinc fingeri | 910 – 932 | C2H2-type 3PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 983 – 1006 | C2H2-type 4PROSITE-ProRule annotationAdd BLAST | 24 | |
Zinc fingeri | 1113 – 1146 | CCHC FOG-type 5PROSITE-ProRule annotationAdd BLAST | 34 |
Keywords - Domaini
Repeat, Zinc-fingerPhylogenomic databases
eggNOGi | ENOG502QQZP, Eukaryota |
GeneTreei | ENSGT00530000063823 |
HOGENOMi | CLU_002277_0_0_1 |
InParanoidi | Q9VPQ6 |
PhylomeDBi | Q9VPQ6 |
Family and domain databases
IDEALi | IID50106 |
InterProi | View protein in InterPro IPR039746, FOG IPR034731, ZF_CCHC_FOG IPR036236, Znf_C2H2_sf IPR013087, Znf_C2H2_type |
PANTHERi | PTHR12958, PTHR12958, 1 hit |
SMARTi | View protein in SMART SM00355, ZnF_C2H2, 8 hits |
SUPFAMi | SSF57667, SSF57667, 6 hits |
PROSITEi | View protein in PROSITE PS51810, ZF_CCHC_FOG, 5 hits PS50157, ZINC_FINGER_C2H2_2, 2 hits |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MLSSNTRGDC SDTAEEMTVD SRDSKDLSAQ DIGEQKQQQM EDQLEDQLND
60 70 80 90 100
SRDPQNNNNN IDDDADEDAE FEEPEKANPQ QDQDLGETEM EQEHDLQQED
110 120 130 140 150
LQQELPANSP STPPRSPSSP QLIPKLEQPA TPPSEPEASP CPSPSPCPTP
160 170 180 190 200
KYPKVRLNAL LASDPALKPD AKELTLPDSR LLAPPPLVKP DTQAQPEVAE
210 220 230 240 250
PLLKPARFMC LPCGIAFSSP STLEAHQAYY CSHRIKDTDE AGSDKSGAGG
260 270 280 290 300
SGATAGDAAG LTGGSTEPPA KMARTGKQYG CTQCSYSADK KVSLNRHMRM
310 320 330 340 350
HQTSPAAPTL AGLPSLLQNG IAPPGVTPNP MEDSSSQQTD RYCSHCDIRF
360 370 380 390 400
NNIKTYRAHK QHYCSSRRPE GQLTPKPDAS PGAGSGPGSA GGSIGVSAQA
410 420 430 440 450
ATPGKLSPQA RNKTPTPAMV AVAAAAAAAA ASLQATPHSH PPFLALPTHP
460 470 480 490 500
IIIVPCSLIR AASFIPGPLP TPNSGIVNPE TTCFTVDNGT IKPLATALVG
510 520 530 540 550
ATLEPERPSA PSSAAEATEA KSSPPEPKRK EAGLTRESAP LDLSLRRSPI
560 570 580 590 600
TLNSLSLRQR QLRNALLDVE EVLLAGVGTG KENVETPRGG GSVTPEQIVC
610 620 630 640 650
APSLPSSPSM SPSPKRRAIS PRSSGAGSAS SMSPPGLNVA VPHLLDMRSM
660 670 680 690 700
LPADFGLSES LLAKTNPELA LKLAAAAAAA AVAGSSGAAA FPPASLPAQT
710 720 730 740 750
SSGNPGSGGS AGGAQQPQIY VKKGVSKCME CNIVFCKYEN YLAHKQHYCS
760 770 780 790 800
ARSQEGASEV DVKSAVSPSI AGAGGLGAGA AEAASSVETT PVAYQQLICA
810 820 830 840 850
ACGIKYTSLD NLRAHQNYYC PKGGAVAAPA ATPTDPGQLG MPKEKCGKCK
860 870 880 890 900
TLHEIGLPCP PPVANPLAAP TVNPQPATNS LNKCPVCGVV SPTAALAKKH
910 920 930 940 950
MEMHGTVKAY RCSICQYKGN TLRGMRTHIR THFDKKTSDV NEELYMTCIF
960 970 980 990 1000
EEDASALSQE LVTPTGASTT TGHDSMDHPS QMFNCDYCNY VSTYKGNVLR
1010 1020 1030 1040 1050
HMKLMHPHVA INSPSISPDT RDQDVTSNPT TNQHSNSDVS NGEAPSFHIK
1060 1070 1080 1090 1100
SEPLDPPPTV NLVHENNNSP IATPHIKAEP IEVGADAAPG GLVPPMTSPL
1110 1120 1130 1140 1150
GNSSSVAAAA AAAAEVMKKY CSTCDISFNY VKTYLAHKQF YCKNKPIRPE
1160 1170 1180 1190
ASDSPSPNHL GGGVAVGLGI GGLVGGHGQQ KNKENLQEAA I
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketM9PDU3 | M9PDU3_DROME | U-shaped, isoform D | ush dFOG, Dmel\CG2762, FOG, l(2)19, u-sh | 1,175 | Annotation score: | ||
M9PC12 | M9PC12_DROME | U-shaped, isoform B | ush dFOG, Dmel\CG2762, FOG, l(2)19, u-sh | 1,198 | Annotation score: | ||
M9PBN1 | M9PBN1_DROME | U-shaped, isoform C | ush dFOG, Dmel\CG2762, FOG, l(2)19, u-sh | 1,212 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 524 | P → H in CAA72991 (PubMed:9367989).Curated | 1 | |
Sequence conflicti | 532 | A → S in CAA72991 (PubMed:9367989).Curated | 1 | |
Sequence conflicti | 1045 | P → A in CAA72991 (PubMed:9367989).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y12322 mRNA Translation: CAA72991.1 AE014134 Genomic DNA Translation: AAF51488.2 BT015278 mRNA Translation: AAT94507.1 |
PIRi | T13850 |
RefSeqi | NP_476780.1, NM_057432.3 |
Genome annotation databases
EnsemblMetazoai | FBtr0078063; FBpp0077723; FBgn0003963 |
GeneIDi | 33225 |
KEGGi | dme:Dmel_CG2762 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y12322 mRNA Translation: CAA72991.1 AE014134 Genomic DNA Translation: AAF51488.2 BT015278 mRNA Translation: AAT94507.1 |
PIRi | T13850 |
RefSeqi | NP_476780.1, NM_057432.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1FU9 | NMR | - | A | 1113-1146 | [»] | |
1FV5 | NMR | - | A | 202-235 | [»] | |
1JN7 | NMR | - | A | 1113-1146 | [»] | |
1Y0J | NMR | - | B | 202-235 | [»] | |
2L6Z | NMR | - | B | 202-235 | [»] | |
AlphaFoldDBi | Q9VPQ6 | |||||
BMRBi | Q9VPQ6 | |||||
SMRi | Q9VPQ6 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 59481, 14 interactors |
IntActi | Q9VPQ6, 1 interactor |
STRINGi | 7227.FBpp0305228 |
PTM databases
iPTMneti | Q9VPQ6 |
Proteomic databases
PaxDbi | Q9VPQ6 |
Protocols and materials databases
DNASUi | 33225 |
Genome annotation databases
EnsemblMetazoai | FBtr0078063; FBpp0077723; FBgn0003963 |
GeneIDi | 33225 |
KEGGi | dme:Dmel_CG2762 |
Organism-specific databases
CTDi | 33225 |
FlyBasei | FBgn0003963, ush |
VEuPathDBi | VectorBase:FBgn0003963 |
Phylogenomic databases
eggNOGi | ENOG502QQZP, Eukaryota |
GeneTreei | ENSGT00530000063823 |
HOGENOMi | CLU_002277_0_0_1 |
InParanoidi | Q9VPQ6 |
PhylomeDBi | Q9VPQ6 |
Enzyme and pathway databases
Reactomei | R-DME-8936459, RUNX1 regulates genes involved in megakaryocyte differentiation and platelet function R-DME-983231, Factors involved in megakaryocyte development and platelet production |
SignaLinki | Q9VPQ6 |
Miscellaneous databases
BioGRID-ORCSi | 33225, 1 hit in 3 CRISPR screens |
ChiTaRSi | ush, fly |
EvolutionaryTracei | Q9VPQ6 |
GenomeRNAii | 33225 |
PROi | PR:Q9VPQ6 |
Gene expression databases
Bgeei | FBgn0003963, Expressed in spermathecum and 61 other tissues |
ExpressionAtlasi | Q9VPQ6, baseline and differential |
Genevisiblei | Q9VPQ6, DM |
Family and domain databases
IDEALi | IID50106 |
InterProi | View protein in InterPro IPR039746, FOG IPR034731, ZF_CCHC_FOG IPR036236, Znf_C2H2_sf IPR013087, Znf_C2H2_type |
PANTHERi | PTHR12958, PTHR12958, 1 hit |
SMARTi | View protein in SMART SM00355, ZnF_C2H2, 8 hits |
SUPFAMi | SSF57667, SSF57667, 6 hits |
PROSITEi | View protein in PROSITE PS51810, ZF_CCHC_FOG, 5 hits PS50157, ZINC_FINGER_C2H2_2, 2 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | USH_DROME | |
Accessioni | Q9VPQ6Primary (citable) accession number: Q9VPQ6 Secondary accession number(s): O18414, Q6AWH0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 15, 2004 |
Last sequence update: | October 1, 2002 | |
Last modified: | May 25, 2022 | |
This is version 172 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families