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Entry version 133 (31 Jul 2019)
Sequence version 2 (01 Mar 2001)
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Protein

DNA primase large subunit

Gene

DNApol-alpha60

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication. Plays a role in the fat facets (faf)-dependent cell-communications pathway.1 Publication

<p>This subsection of the ‘Function’ section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the ‘Description’ field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi298Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi377Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi393Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi433Iron-sulfur (4Fe-4S)By similarity1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

  • DNA replication Source: FlyBase
  • DNA-dependent DNA replication Source: FlyBase
  • compound eye morphogenesis Source: UniProtKB
  • mitotic cell cycle Source: UniProtKB

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionDNA-binding, Nucleotidyltransferase, Transferase
Biological processDNA replication, Transcription
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

Reactome - a knowledgebase of biological pathways and processes

More...
Reactomei
R-DME-113501 Inhibition of replication initiation of damaged DNA by RB1/E2F1
R-DME-174411 Polymerase switching on the C-strand of the telomere
R-DME-174430 Telomere C-strand synthesis initiation
R-DME-68952 DNA replication initiation
R-DME-68962 Activation of the pre-replicative complex
R-DME-69091 Polymerase switching
R-DME-69166 Removal of the Flap Intermediate
R-DME-69183 Processive synthesis on the lagging strand

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
DNA primase large subunit (EC:2.7.7.-)
Alternative name(s):
Suppressor of fat facets 240
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:DNApol-alpha60
Synonyms:DNAprim, S(faf)240
ORF Names:CG5553
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiDrosophila melanogaster (Fruit fly)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri7227 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000803 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 3L

Organism-specific databases

Drosophila genome database

More...
FlyBasei
FBgn0259676 DNApol-alpha60

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

Keywords - Cellular componenti

DNA-directed RNA polymerase, Primosome

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This subsection of the ‘Pathology and Biotech’ section describes the in vivo effects caused by ablation of the gene (or one or more transcripts) coding for the protein described in the entry. This includes gene knockout and knockdown, provided experiments have been performed in the context of a whole organism or a specific tissue, and not at the single-cell level.<p><a href='/help/disruption_phenotype' target='_top'>More...</a></p>Disruption phenotypei

Mutants exhibit eye defects and a delay in the S- phase of the cell cycle.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology_and_biotech_section">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi136E → K in DNApol-alpha60-T2; pupal lethal. 1 Publication1

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000467721 – 533DNA primase large subunitAdd BLAST533

Proteomic databases

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
Q9VPH2

PRoteomics IDEntifications database

More...
PRIDEi
Q9VPH2

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Bgee dataBase for Gene Expression Evolution

More...
Bgeei
FBgn0259676 Expressed in 18 organ(s), highest expression level in embryo

ExpressionAtlas, Differential and Baseline Expression

More...
ExpressionAtlasi
Q9VPH2 baseline and differential

Genevisible search portal to normalized and curated expression data from Genevestigator

More...
Genevisiblei
Q9VPH2 DM

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Heterodimer of a small subunit and a large subunit.

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGrid)

More...
BioGridi
69380, 13 interactors

ComplexPortal: manually curated resource of macromolecular complexes

More...
ComplexPortali
CPX-2090 DNA polymerase alpha:primase complex

Protein interaction database and analysis system

More...
IntActi
Q9VPH2, 9 interactors

STRING: functional protein association networks

More...
STRINGi
7227.FBpp0077899

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
KOG2267 Eukaryota
COG2219 LUCA

Ensembl GeneTree

More...
GeneTreei
ENSGT00390000009790

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
Q9VPH2

KEGG Orthology (KO)

More...
KOi
K02685

Identification of Orthologs from Complete Genome Data

More...
OMAi
DCHGCPY

Database of Orthologous Groups

More...
OrthoDBi
479347at2759

Database for complete collections of gene phylogenies

More...
PhylomeDBi
Q9VPH2

Family and domain databases

Conserved Domains Database

More...
CDDi
cd07322 PriL_PriS_Eukaryotic, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR016558 DNA_primase_lsu_euk
IPR007238 DNA_primase_lsu_euk/arc

The PANTHER Classification System

More...
PANTHERi
PTHR10537 PTHR10537, 1 hit

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF04104 DNA_primase_lrg, 1 hit

PIRSF; a whole-protein classification database

More...
PIRSFi
PIRSF009449 DNA_primase_large_subunit, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

Q9VPH2-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEFGLKKRAR HEVKVEVASL ETKYPHNVML YHYPPTEDVH IDEFEELALE
60 70 80 90 100
RLRLLRVLDR ASTRNLRVLS DEWKEIVSAD LTREGLRSYL RLCSTGGSAK
110 120 130 140 150
HEADIQTRRR DYLSHFILRL AYCRSEDLAR WFVAREMELF RYKFAALSSF
160 170 180 190 200
EVKQFLEANG FEIHPLTEAQ KDEVKDGLYE STVGQSVAKI ELLDFYKVPF
210 220 230 240 250
TQVLDLVRGR RCFLKAGYAY VNTHDLVSLV GTKQQDEIEQ GLQAAKTMVE
260 270 280 290 300
DVEADERISR TLKALHNSYT GRDYTVCRDA AVPIESLDQL SKTSMPLCMR
310 320 330 340 350
MCHEHIRANH HIKHSGRMQY GLFLKGIGVA LEDSLRFWRE EFTKKMDADK
360 370 380 390 400
FTRSYEYNIY HNYGKKGSMV NYTPYSCAKI IKDVAGPGDC HGCPYKNMDQ
410 420 430 440 450
GSLKTKLSSY GLSASAIDEV MFFVSRGHYQ IGCGKYFQLT HNSPVEPSIN
460 470 480 490 500
HPNNYFEESQ IAMGNRQKRA NGSAPPKARI RPDIKGHGDR SMLMGDDDDE
510 520 530
LWRIAETQER IMQSQKDISE AFNDDLDLTQ IDY
Length:533
Mass (Da):61,436
Last modified:March 1, 2001 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i92CE6049AA8685E5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Sequence’ section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti142Y → F in AAD46835 (PubMed:10731138).Curated1
Sequence conflicti149S → G in AAD46835 (PubMed:10731138).Curated1

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
AF291873 Genomic DNA Translation: AAG01548.1
AE014296 Genomic DNA Translation: AAF51580.2
AF160895 mRNA Translation: AAD46835.1

NCBI Reference Sequences

More...
RefSeqi
NP_652001.2, NM_143744.4

Genome annotation databases

Ensembl metazoan genome annotation project

More...
EnsemblMetazoai
FBtr0078241; FBpp0077899; FBgn0259676

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
44915

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
dme:Dmel_CG5553

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF291873 Genomic DNA Translation: AAG01548.1
AE014296 Genomic DNA Translation: AAF51580.2
AF160895 mRNA Translation: AAD46835.1
RefSeqiNP_652001.2, NM_143744.4

3D structure databases

Database of comparative protein structure models

More...
ModBasei
Search...

SWISS-MODEL Interactive Workspace

More...
SWISS-MODEL-Workspacei
Submit a new modelling project...

Protein-protein interaction databases

BioGridi69380, 13 interactors
ComplexPortaliCPX-2090 DNA polymerase alpha:primase complex
IntActiQ9VPH2, 9 interactors
STRINGi7227.FBpp0077899

Proteomic databases

PaxDbiQ9VPH2
PRIDEiQ9VPH2

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0078241; FBpp0077899; FBgn0259676
GeneIDi44915
KEGGidme:Dmel_CG5553

Organism-specific databases

Comparative Toxicogenomics Database

More...
CTDi
44915
FlyBaseiFBgn0259676 DNApol-alpha60

Phylogenomic databases

eggNOGiKOG2267 Eukaryota
COG2219 LUCA
GeneTreeiENSGT00390000009790
InParanoidiQ9VPH2
KOiK02685
OMAiDCHGCPY
OrthoDBi479347at2759
PhylomeDBiQ9VPH2

Enzyme and pathway databases

ReactomeiR-DME-113501 Inhibition of replication initiation of damaged DNA by RB1/E2F1
R-DME-174411 Polymerase switching on the C-strand of the telomere
R-DME-174430 Telomere C-strand synthesis initiation
R-DME-68952 DNA replication initiation
R-DME-68962 Activation of the pre-replicative complex
R-DME-69091 Polymerase switching
R-DME-69166 Removal of the Flap Intermediate
R-DME-69183 Processive synthesis on the lagging strand

Miscellaneous databases

Database of phenotypes from RNA interference screens in Drosophila and Homo sapiens

More...
GenomeRNAii
44915

Protein Ontology

More...
PROi
PR:Q9VPH2

Gene expression databases

BgeeiFBgn0259676 Expressed in 18 organ(s), highest expression level in embryo
ExpressionAtlasiQ9VPH2 baseline and differential
GenevisibleiQ9VPH2 DM

Family and domain databases

CDDicd07322 PriL_PriS_Eukaryotic, 1 hit
InterProiView protein in InterPro
IPR016558 DNA_primase_lsu_euk
IPR007238 DNA_primase_lsu_euk/arc
PANTHERiPTHR10537 PTHR10537, 1 hit
PfamiView protein in Pfam
PF04104 DNA_primase_lrg, 1 hit
PIRSFiPIRSF009449 DNA_primase_large_subunit, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiPRI2_DROME
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q9VPH2
Secondary accession number(s): Q9U9Q5
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 29, 2003
Last sequence update: March 1, 2001
Last modified: July 31, 2019
This is version 133 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
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