UniProtKB - Q9VMD9 (TIG_DROME)
Protein
Tiggrin
Gene
Tig
Organism
Drosophila melanogaster (Fruit fly)
Status
Functioni
Functions as a ligand for integrin alpha-PS2/beta-PS. Required in larvae for proper muscle structure and function. Involved in the regulation of cell adhesion during wing development.4 Publications
GO - Molecular functioni
- integrin binding Source: FlyBase
GO - Biological processi
- axon guidance Source: FlyBase
- cell adhesion mediated by integrin Source: FlyBase
- cell-substrate adhesion Source: FlyBase
- photoreceptor cell axon guidance Source: FlyBase
- regulation of cell adhesion mediated by integrin Source: FlyBase
- substrate adhesion-dependent cell spreading Source: FlyBase
Keywordsi
Molecular function | Developmental protein |
Names & Taxonomyi
Protein namesi | Recommended name: TiggrinImported |
Gene namesi | Name:TigImported ORF Names:CG11527 |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Holometabola › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0011722, Tig |
Subcellular locationi
Extracellular region or secreted
- extracellular matrix 2 Publications
Note: Localizes to the basal surface of the dorsoventral cell layer interface during pupal wing development.2 Publications
Extracellular region or secreted
- basement membrane Source: FlyBase
- extracellular matrix Source: FlyBase
Keywords - Cellular componenti
Extracellular matrix, SecretedPathology & Biotechi
Disruption phenotypei
Pupal lethal with about 1% escapers. In mutant larvae, muscles 6 and 7 appear stringy and not anchored to other muscles or the epidermis, often these muscles are missing or unrecognizable. Sites where muscles 3, 4, 5, 8 and 16 come together are rarely recognizable and large gaps between muscles 9 and 10 can be observed. Muscle contraction waves that transverse the length of the larvae and are responsible for locomotion are much slower in mutant larvae. Mutant pupae are longer than wild type pupae. Mutant adult flies present elongated abdomen and wings with altered shapes and sizes.2 Publications
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 1989 – 1991 | RGD → LGA: Partially rescues muscle function and structure defects in the null mutant. Fails to interact with alpha-PS2/beta-PS. 1 Publication | 3 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 18 | 1 PublicationAdd BLAST | 18 | |
ChainiPRO_5000144152 | 19 – 2188 | Tiggrin1 PublicationAdd BLAST | 2170 |
Post-translational modificationi
O-glycosylation by pgant3 is required for proper secretion and localization to the basal cell layer interface during wing development.2 Publications
Proteomic databases
PaxDbi | Q9VMD9 |
PRIDEi | Q9VMD9 |
Expressioni
Tissue specificityi
In embryos, expressed in the apodemes (muscle attachment sites) of the major longitudinal muscles 4, 6, 7, 12 and 13 and the wide dorsal oblique muscles 9 and 10, in hemocytes, in fat body cells, in basement membranes surrounding the gut and in the commissures of the ventral nerve cord. Expressed in larval imaginal wing disk and in pupal wing. In adult flies, expressed in the jump muscle (at protein level).5 Publications
Developmental stagei
Expressed throughout embryogenesis; first appears at 8-10 hours. Detected in larval and pupal stages (at protein level). Transcripts are first detected at 6-8 hours of development, expression peaks at 12-14 hours and then declines by the end of embryogenesis.2 Publications
Gene expression databases
Bgeei | FBgn0011722, Expressed in arthropod fat body and 33 other tissues |
ExpressionAtlasi | Q9VMD9, baseline and differential |
Genevisiblei | Q9VMD9, DM |
Interactioni
GO - Molecular functioni
- integrin binding Source: FlyBase
Protein-protein interaction databases
BioGRIDi | 60053, 3 interactors |
IntActi | Q9VMD9, 12 interactors |
STRINGi | 7227.FBpp0078905 |
Family & Domainsi
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 570 – 635 | Sequence analysisAdd BLAST | 66 | |
Coiled coili | 1009 – 1050 | Sequence analysisAdd BLAST | 42 | |
Coiled coili | 1312 – 1343 | Sequence analysisAdd BLAST | 32 | |
Coiled coili | 1613 – 1641 | Sequence analysisAdd BLAST | 29 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 1989 – 1991 | Cell attachment site2 Publications | 3 |
Keywords - Domaini
Coiled coil, SignalPhylogenomic databases
eggNOGi | ENOG502S4BN, Eukaryota |
HOGENOMi | CLU_231371_0_0_1 |
InParanoidi | Q9VMD9 |
OMAi | QNKQWSA |
OrthoDBi | 16764at2759 |
PhylomeDBi | Q9VMD9 |
Family and domain databases
InterProi | View protein in InterPro IPR016084, Haem_Oase-like_multi-hlx |
SUPFAMi | SSF48613, SSF48613, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q9VMD9-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRALGGITLL LAVAICQGYE TYQRSSFRSS SSSSYGGGQT VPQLNSFASA
60 70 80 90 100
HFNEVRELAN QLKQKFNVLS QGSTNFAYTS PWSASILDLS GKSTLQLDQL
110 120 130 140 150
SSEISRQLVQ DMREGITNYH TIAQPNFFEA KAAELLERYS GAESASLQQT
160 170 180 190 200
VGLGPYQPVD LSGFDEVKNY AYPAEVKVID GKTYVVHRNC TEATKLSDYG
210 220 230 240 250
SSGQLNSGFL GHQQTSLPLS STTTTITRKK TIHDWVRENM EPSVVGYNSV
260 270 280 290 300
VKLDGQLRNS ALNQMVPLSP GSNVVIHRFN KTITTNPDGT SSVGGSEWQQ
310 320 330 340 350
RWQDGKLVYD HQQPFGQSTI PRDEQWKREE RERLFWYLTT PQRLDDWQQQ
360 370 380 390 400
QEERLLGVVQ RYQVSLPVLK EFHRRELARY EALLGQYQSR VQDTSSWQRQ
410 420 430 440 450
ERGRLDWLIH QNGFTVQDIE RWQNENARKL AEAARQHGIS QNQLQQFQRE
460 470 480 490 500
ELQRLYVHFN QVNESLAPQV PSVPQTTYNY QSSSSLTEDN TKEQQRLEEL
510 520 530 540 550
IRQHNATIAA LQNSIKTDQQ RLKNLSIKYQ GDMQSQTQWL RGEVARIGDL
560 570 580 590 600
IKEQNEQVSK ITAWQSSERS RLENILLQHR GSVEEVQQRI NMDRNYLQNL
610 620 630 640 650
ATKYQVSVEE LEKWQKEELE RLQVRGQQQL EEHIKDWQIS VSSNLRDIAT
660 670 680 690 700
QNKLTIDEFQ NYIINDRSHL EEMARLYKVK VEEIEQWIKS ELKKFQSEGL
710 720 730 740 750
LKGVEQELIQ WQQKERERLQ AIVQQNSLTV EQLEVRIKND QDHFFKLADK
760 770 780 790 800
YKINVEDIQD WLKKELLRLQ SEGLVKAETL KEWQQQERAQ ISLLVQQNKY
810 820 830 840 850
SLDEFERKML ADRARLQELS NTYNVKVSEI EQWIKSEGDR LQHEGQLRME
860 870 880 890 900
SQLNNWQKIE RQRLLDLINK NNLSIEEIES KISKDQTHLY SLAQQHQVRV
910 920 930 940 950
EEIEQWIRQQ IQKLQDQGLI EMQKLKNWQL EWRGNLTNMV QDRDFTVEEF
960 970 980 990 1000
HKWLLKDREQ LQSLAMQHNV QIEEIEQFVK KEEQRFIGMG LLKPSEKLTN
1010 1020 1030 1040 1050
WQEVERLHLK NLAQQQYKST EQLEARLRQD RELLERLARQ YSVQVEEIES
1060 1070 1080 1090 1100
WMKQELARMR DEGQLQIDNL TSWQLAERER LEALIKQNKQ WSAEELRAEL
1110 1120 1130 1140 1150
EKDREHMQTM AFQYHTSVEE IEKWLQSEIE RLKQQGKLNI EQLTAWQRTE
1160 1170 1180 1190 1200
QQRILSLLQQ HSNITLEQFQ AKVHNDRRFL MNLAEQHHVH IEEVDNYVKQ
1210 1220 1230 1240 1250
VIEDLRKNGQ FEIEQLQTWQ RVERDYIKSL ISEYKNSLST AEYEEKLLAD
1260 1270 1280 1290 1300
RAHLKHLADQ YRINVEQIEE WMIAELKRLR GSTEETLKSL SAWQVSELER
1310 1320 1330 1340 1350
LQNLVKQQNH LTFVEFEMEL NQERDRLQKL ANQYSVNVVE IEEWLRQQLI
1360 1370 1380 1390 1400
NLRTTGQAKV ENLSKWQVEE QQRLIEMLLK KQQEMPYEQV ERELTQDHAR
1410 1420 1430 1440 1450
LQSLSQTHHV DIDHVDHWLR EELRRLQSSG LVQIEQQTQW QQKISNGFNN
1460 1470 1480 1490 1500
WLEQQRNGAS YQDFVDFLKR DKQRMDGIAT DYHVTVEQVE KWVQKEAARL
1510 1520 1530 1540 1550
SLIGVIERPE NNLKYEDISN IWVGDQTDSW KNELVTRLRS VTRQRPFTRQ
1560 1570 1580 1590 1600
EFESYLIRNK PIFEQIARQY HVTIEDIHLW LDQSAKNEGL VTTEWQAKER
1610 1620 1630 1640 1650
LHIDNLINQQ LRKQQRWTIE ELELRLNNDQ KHLQDAVAQY HVTVEELKVW
1660 1670 1680 1690 1700
YKDELNRLLE QRRIDRGSGI SWQNIESQRI YLAIVNNPGI SRQALENRLF
1710 1720 1730 1740 1750
RDVHVRASQY QITVEELRQF ILSQLRRFSD MGLIVDNGRQ ANNWHDQERK
1760 1770 1780 1790 1800
RLREVVKGVV ITEQELLDFI SQDTSFQTQL AQSYQVGLEQ LAPVQRIFIG
1810 1820 1830 1840 1850
NLAREQLLEQ RRLNHLTTWQ QRERDRLYEF IGNQNMTQTE LKTWQIQDSK
1860 1870 1880 1890 1900
LLAEFAKRYE ISVQQLSDWQ KKELARINQL ARYYGMSQSD LQQFREGELR
1910 1920 1930 1940 1950
QLAYINHRQL LSAAEAQKWE KRHQWTLSRL QSRYGKFGQE LVAWRRTLYL
1960 1970 1980 1990 2000
LSQGLIDLPA DSGSNGGYVV DAGSTNATAV YKPIFSKDRG DQPPHTYDES
2010 2020 2030 2040 2050
FVEGDEPGLE GETARPRPPN PAPIVSTPKP PLPYSRGGPS GGFEYRRQDY
2060 2070 2080 2090 2100
TFNVPVGSAS ASASGGPTGS SASASASLGK WNRASGDEPL QQEVDLGQQQ
2110 2120 2130 2140 2150
QIEELGWNEK LEDLGQQTQV EDTDWNQQAE DLGQQQQVQV EDDLHFDQTQ
2160 2170 2180
GHSSSSNSRS QPLQQATKVE VEATSEPSFW EKLKEKLG
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketX2JDD7 | X2JDD7_DROME | Tiggrin, isoform B | Tig CT36389, Dmel\CG11527, TIG, tig, CG11527 | 2,188 | Annotation score: |
Sequence cautioni
The sequence AAQ23578 differs from that shown. Reason: Frameshift.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 7 | I → V in AAA56998 (PubMed:7924982).Curated | 1 | |
Sequence conflicti | 77 | A → G in AAA56998 (PubMed:7924982).Curated | 1 | |
Sequence conflicti | 381 | E → Q in AAA56998 (PubMed:7924982).Curated | 1 | |
Sequence conflicti | 869 | N → S in AAA56998 (PubMed:7924982).Curated | 1 | |
Sequence conflicti | 2157 – 2158 | Missing in AAA56998 (PubMed:7924982).Curated | 2 | |
Sequence conflicti | 2185 | E → G in AAA56998 (PubMed:7924982).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U09506 mRNA Translation: AAA56998.1 AE014134 Genomic DNA Translation: AAF52380.2 BT010260 mRNA Translation: AAQ23578.1 Frameshift. |
RefSeqi | NP_001285670.1, NM_001298741.1 NP_477033.1, NM_057685.4 |
Genome annotation databases
EnsemblMetazoai | FBtr0079275; FBpp0078905; FBgn0011722 FBtr0343152; FBpp0309850; FBgn0011722 |
GeneIDi | 33896 |
KEGGi | dme:Dmel_CG11527 |
UCSCi | CG11527-RA, d. melanogaster |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U09506 mRNA Translation: AAA56998.1 AE014134 Genomic DNA Translation: AAF52380.2 BT010260 mRNA Translation: AAQ23578.1 Frameshift. |
RefSeqi | NP_001285670.1, NM_001298741.1 NP_477033.1, NM_057685.4 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 60053, 3 interactors |
IntActi | Q9VMD9, 12 interactors |
STRINGi | 7227.FBpp0078905 |
Proteomic databases
PaxDbi | Q9VMD9 |
PRIDEi | Q9VMD9 |
Genome annotation databases
EnsemblMetazoai | FBtr0079275; FBpp0078905; FBgn0011722 FBtr0343152; FBpp0309850; FBgn0011722 |
GeneIDi | 33896 |
KEGGi | dme:Dmel_CG11527 |
UCSCi | CG11527-RA, d. melanogaster |
Organism-specific databases
CTDi | 33896 |
FlyBasei | FBgn0011722, Tig |
Phylogenomic databases
eggNOGi | ENOG502S4BN, Eukaryota |
HOGENOMi | CLU_231371_0_0_1 |
InParanoidi | Q9VMD9 |
OMAi | QNKQWSA |
OrthoDBi | 16764at2759 |
PhylomeDBi | Q9VMD9 |
Miscellaneous databases
BioGRID-ORCSi | 33896, 0 hits in 1 CRISPR screen |
GenomeRNAii | 33896 |
PROi | PR:Q9VMD9 |
Gene expression databases
Bgeei | FBgn0011722, Expressed in arthropod fat body and 33 other tissues |
ExpressionAtlasi | Q9VMD9, baseline and differential |
Genevisiblei | Q9VMD9, DM |
Family and domain databases
InterProi | View protein in InterPro IPR016084, Haem_Oase-like_multi-hlx |
SUPFAMi | SSF48613, SSF48613, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TIG_DROME | |
Accessioni | Q9VMD9Primary (citable) accession number: Q9VMD9 Secondary accession number(s): Q23984, Q6NR23 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 16, 2013 |
Last sequence update: | March 1, 2001 | |
Last modified: | August 12, 2020 | |
This is version 130 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase