UniProtKB - Q9VMA3 (CUP_DROME)
Protein cup
cup
Functioni
Adapter protein that plays a central role in localization of transcripts in the oocyte and in young embryos (PubMed:9118812).
Maintains RNA targets in a repressed state by promoting their deadenylation and protects deadenylated mRNAs from further degradation (PubMed:21937713).
Binds to and recruits eIF-4E to the 3'-UTR of some mRNA targets which prevents interaction between eIF4E1 and eIF4G (PubMed:14685270, PubMed:15465908, PubMed:21081899).
This may contribute to translational repression but does not appear to be necessary for it to occur (PubMed:21081899).
Can promote translational repression independently of deadenylation and eIF4E1 binding (PubMed:21937713).
Required for correct localization of eIF4E1 in the developing oocyte (PubMed:15465908).
Required for translational repression of oskar (osk) mRNA (PubMed:14691132, PubMed:14723848).
Also required for the translational repression of nanos (nos) mRNA (PubMed:21081899).
Promotes the accumulation of the germ plasm components osk, vas and stau at the posterior pole of the oocyte and is required for germ cell development (PubMed:22454519, PubMed:17277377).
Represses orb positive autoregulatory activity which prevents premature activation of orb and ensures its accumulation specifically in the developing oocyte (PubMed:22164257).
In 0-1 hour embryos, forms a complex with me31B, cup, tral and pAbp which binds to various mRNAs including maternal mRNAs, and down-regulates their expression during the maternal-to-zygotic transition (PubMed:28875934).
11 PublicationsGO - Molecular functioni
- eukaryotic initiation factor 4E binding Source: FlyBase
- mRNA binding Source: GO_Central
- RNA binding Source: FlyBase
- translation regulator activity Source: UniProtKB
GO - Biological processi
- dorsal/ventral pattern formation Source: FlyBase
- female meiotic nuclear division Source: UniProtKB
- intracellular mRNA localization Source: FlyBase
- meiotic chromosome segregation Source: UniProtKB
- negative regulation of eukaryotic translation initiation factor 4F complex assembly Source: FlyBase
- negative regulation of oskar mRNA translation Source: FlyBase
- negative regulation of translation Source: FlyBase
- negative regulation of translational initiation Source: FlyBase
- oocyte growth in germarium-derived egg chamber Source: UniProtKB
- oogenesis Source: FlyBase
- ovarian follicle cell migration Source: FlyBase
- regulation of pole plasm oskar mRNA localization Source: FlyBase
- regulation of synaptic assembly at neuromuscular junction Source: FlyBase
Keywordsi
Molecular function | Developmental protein, Repressor |
Biological process | Differentiation, Oogenesis, Transcription, Transcription regulation |
Enzyme and pathway databases
SignaLinki | Q9VMA3 |
Names & Taxonomyi
Protein namesi | Recommended name: Protein cupAlternative name(s): Oskar ribonucleoprotein complex 147 kDa subunit |
Gene namesi | Name:cup Synonyms:fs(2)cup ORF Names:CG11181 |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0000392, cup |
VEuPathDBi | VectorBase:FBgn0000392 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 4 Publications
Nucleus
- Nucleus 2 Publications
Other locations
- Cytoplasmic ribonucleoprotein granule 1 Publication
Note: Retained in the cytoplasm by interaction with eIF4E1. Located throughout the cytoplasm of all germline cells and is excluded from the nucleus of nurse cells, oocytes and follicular cells (PubMed:15465908). During oogenesis, accumulates in the cytoplasm at the posterior end of the forming oocyte (PubMed:15465908). Component of the germ plasm in developing oocytes (PubMed:22454519). Does not localizes in processing bodies (P-bodies) (PubMed:24335285).3 Publications
Cytosol
- cytosol Source: FlyBase
Nucleus
- nucleus Source: FlyBase
Other locations
- cytoplasm Source: FlyBase
- messenger ribonucleoprotein complex Source: UniProtKB
- neuromuscular junction Source: FlyBase
- neuronal ribonucleoprotein granule Source: UniProtKB
- P-body Source: UniProtKB
- terminal bouton Source: FlyBase
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Disruption phenotypei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 327 | Y → A: Strong reduction in interaction with eIF4E1. Strong reduction in interaction with eIF4E1; when associated with A-332 and A-348. Complete loss of interaction with eIF4E1; when associated with A-364 and A-368. 2 Publications | 1 | |
Mutagenesisi | 332 – 333 | LM → AA: Reduction in interaction with eIF4E1. Strong reduction in interaction with eIF4E1; when associated with A-327. 1 Publication | 2 | |
Mutagenesisi | 364 | L → A: Mild reduction in interaction with eIF4E1. Complete loss of interaction with eIF4E1; when associated with A-327 and A-368. 1 Publication | 1 | |
Mutagenesisi | 368 | L → A: Mild reduction in interaction with eIF4E1. Complete loss of interaction with eIF4E1; when associated with A-327 and A-364. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000079561 | 1 – 1117 | Protein cupAdd BLAST | 1117 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 263 | Phosphoserine1 Publication | 1 | |
Modified residuei | 270 | Phosphoserine1 Publication | 1 | |
Modified residuei | 347 | Phosphoserine1 Publication | 1 | |
Modified residuei | 350 | Phosphoserine1 Publication | 1 | |
Modified residuei | 503 | Phosphothreonine1 Publication | 1 | |
Modified residuei | 509 | Phosphoserine1 Publication | 1 | |
Modified residuei | 513 | Phosphoserine1 Publication | 1 | |
Modified residuei | 520 | Phosphoserine1 Publication | 1 | |
Modified residuei | 523 | Phosphoserine1 Publication | 1 | |
Modified residuei | 524 | Phosphoserine1 Publication | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | Q9VMA3 |
PTM databases
iPTMneti | Q9VMA3 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | FBgn0000392, Expressed in embryo and 21 other tissues |
Genevisiblei | Q9VMA3, DM |
Interactioni
Subunit structurei
Component of the osk RNP complex, which is composed of at least exu, yps, aret/bruno, cup, and the mRNA of osk (PubMed:10662770).
Interacts with the decapping activators me31B and tral (PubMed:21937713).
Component of the nos RNP complex, which is composed of at least smg, cup, tral, me31B, the CCR4-NOT complex members Rga/NOT2 and Caf1, and the mRNA of nos (PubMed:21081899).
Interacts with btz (PubMed:14691132, PubMed:14723848). Recruited to the 3'-UTR of nos and osk mRNAs by smg and btz, respectively (PubMed:14685270, PubMed:14691132).
Forms a ribonucleoprotein complex (RNP) containing at least me31B, eIF4E1, cup, tral and pAbp; this interaction is required for the translational silencing of maternal mRNAs during the maternal-to-zygotic transition (PubMed:28875934). No interaction was detected with pAbp in 1-5 hour embryos (PubMed:28875934).
Interacts with osk and vas (PubMed:22454519).
Interacts with Pop2, twin/CCR4, Rga, Not3 and Not1 which are all core components of the CCR4-NOT deadenylase complex; interaction with the complex is required for cup deadenylation activity (PubMed:21937713).
Interacts with nos (PubMed:11060247).
Interacts with smg (PubMed:14685270).
Interacts (via YXXXXLphi motifs) with eIF4E1; the interaction promotes retention of cup in the cytoplasm (PubMed:14723848, PubMed:14685270, PubMed:15465908, PubMed:21937713, PubMed:22832024).
Interacts with orb; the interaction represses the orb positive autoregulatory loop (PubMed:22164257).
Interacts with Nup154 (PubMed:17277377).
13 PublicationsBinary interactionsi
Q9VMA3
With | #Exp. | IntAct |
---|---|---|
eIF4E1 [P48598] | 6 | EBI-95398,EBI-198574 |
me31B [P23128] | 3 | EBI-95398,EBI-300281 |
smg [Q23972] | 4 | EBI-95398,EBI-108638 |
GO - Molecular functioni
- eukaryotic initiation factor 4E binding Source: FlyBase
Protein-protein interaction databases
BioGRIDi | 60088, 64 interactors |
ComplexPortali | CPX-3177, eIF4E-cup complex |
DIPi | DIP-17920N |
ELMi | Q9VMA3 |
IntActi | Q9VMA3, 10 interactors |
MINTi | Q9VMA3 |
STRINGi | 7227.FBpp0288761 |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | Q9VMA3 |
SMRi | Q9VMA3 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 106 | DisorderedSequence analysisAdd BLAST | 106 | |
Regioni | 270 – 326 | DisorderedSequence analysisAdd BLAST | 57 | |
Regioni | 493 – 528 | DisorderedSequence analysisAdd BLAST | 36 | |
Regioni | 596 – 618 | DisorderedSequence analysisAdd BLAST | 23 | |
Regioni | 654 – 673 | DisorderedSequence analysisAdd BLAST | 20 | |
Regioni | 679 – 728 | DisorderedSequence analysisAdd BLAST | 50 | |
Regioni | 984 – 1004 | DisorderedSequence analysisAdd BLAST | 21 | |
Regioni | 1016 – 1051 | DisorderedSequence analysisAdd BLAST | 36 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 327 – 333 | YXXXXLphi motif 12 Publications | 7 | |
Motifi | 363 – 369 | YXXXXLphi motif 21 Publication | 7 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 51 – 66 | Pro residuesSequence analysisAdd BLAST | 16 | |
Compositional biasi | 77 – 91 | Basic and acidic residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 279 – 297 | Polar residuesSequence analysisAdd BLAST | 19 | |
Compositional biasi | 493 – 517 | Polar residuesSequence analysisAdd BLAST | 25 | |
Compositional biasi | 1023 – 1051 | Polar residuesSequence analysisAdd BLAST | 29 |
Domaini
Sequence similaritiesi
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | ENOG502SXKW, Eukaryota |
HOGENOMi | CLU_278765_0_0_1 |
InParanoidi | Q9VMA3 |
OMAi | MGMSNNR |
PhylomeDBi | Q9VMA3 |
Family and domain databases
DisProti | DP01494 |
IDEALi | IID50295 |
InterProi | View protein in InterPro IPR018862, eIF4E-T |
PANTHERi | PTHR12269, PTHR12269, 2 hits |
Pfami | View protein in Pfam PF10477, EIF4E-T, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MQMAEAEQEN GAGALKIATN AGATDRPAHQ QLPLPVEDQQ DEVLTPAEKG
60 70 80 90 100
KFEYPPPPPP PTPVQAPLAT KATALNASQE HDDDEANSEK WEDPCAPPPP
110 120 130 140 150
PPLPTSAFLA TGLGYLKLPA FKLKDALEKA ITKLEANKRT LKASPESSRS
160 170 180 190 200
IKNKNVVALE MLPRRSNPET IGDGSMLAST STAVMLQTKK PAVIVEMERR
210 220 230 240 250
CKIINLLAKQ NQILESISGE AIPMHGPSKH LHEDEGLTLQ VLSARASTPY
260 270 280 290 300
TQPSSMLSCT AVSCDLEHDS PRKQVASKEA VPEQQSSQVQ QKRPPSTGIH
310 320 330 340 350
KPGSLRAPKA VRPTTAPVVS SKPVKSYTRS RLMDIRNGMF NALMHRSKES
360 370 380 390 400
FVMPRIATCD DIELEGRLRR MNIWRTSDGT RFRTRSTTAN LNMNNNNNNE
410 420 430 440 450
CMPAFFKNKN KPNLISDESI IQSQPPQPQT EFQDPAIVNQ RRIGSGRLNH
460 470 480 490 500
SKWGYNDEDY HSYHNGKSQH MEEVNSKNSK NMTVLQFFDN GEISSQPQRR
510 520 530 540 550
PNTPVMGMSI NRSENDTLHS NESSEDLSRA NENYVKRVMS GFLVVSKPKS
560 570 580 590 600
RDVEDRHHRR YRNQNEEPEW FSCGPTSRLD TIELCGFDED EEKMLKEGNK
610 620 630 640 650
NHGLGETERE TSKQKMDHKY KWTHAEPMGR SKYMPKHDTN NNHNVENMNN
660 670 680 690 700
VMATEHQQQK EEKRPGSGRS FQFDKFNQSQ QNYESSSYVN HQQPPQTQPQ
710 720 730 740 750
QMQQQSNTNT NNSKFMSFFA NEGNSSSSSL NEFFKQAINQ GHGNNPEQPK
760 770 780 790 800
SLGHIGQMPS VDQLEAKWRR NSLNNVGETA NKQTDNFQKL IGSLSSAKPQ
810 820 830 840 850
SQAVGYDAIS NFIMQQQQYQ QQQQKQHLII QQQQQHTAFL ASLQLKAILG
860 870 880 890 900
RADTQLLLLR LTKGEISKHG LLVQLANPRL TDMDREAITA VLQFTNTQQQ
910 920 930 940 950
QQQHKQQLDM LSSTVIASQL QNLHNLAIVQ QTLAARQQPQ HNPQTQAPHQ
960 970 980 990 1000
LSQEDLQAHA NVIMRNAVMK RKIEEQTSKL INGGAKHQAQ QQYLNRGQQR
1010 1020 1030 1040 1050
QARPDANSNA LLHALISGGG NNHASGYPMN GQPQKHHSNL RFGDNQNFQS
1060 1070 1080 1090 1100
FESNQPHFAT QYKQQYQQSQ QQHPHQQPQQ LNSLHQNNAG AVNSFNKAQM
1110
QAQSAISMLP NSGDEFH
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketX2J9P9 | X2J9P9_DROME | Cup, isoform C | cup CUP, Cup, DmCup, Dmel\CG11181, fs(1)cup | 1,117 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 836 | H → Q in AAB64427 (PubMed:9118812).Curated | 1 | |
Sequence conflicti | 1030 | N → K in AAB64427 (PubMed:9118812).Curated | 1 | |
Sequence conflicti | 1035 | K → Q in AAB64427 (PubMed:9118812).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF004917 mRNA Translation: AAB64427.1 Different initiation. AE014134 Genomic DNA Translation: AAF52418.3 AY069707 mRNA Translation: AAL39852.1 Different initiation. |
RefSeqi | NP_001285687.1, NM_001298758.1 NP_523493.3, NM_078769.3 |
Genome annotation databases
EnsemblMetazoai | FBtr0290322; FBpp0288761; FBgn0000392 FBtr0346452; FBpp0312102; FBgn0000392 |
GeneIDi | 33934 |
KEGGi | dme:Dmel_CG11181 |
UCSCi | CG11181-RB, d. melanogaster |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF004917 mRNA Translation: AAB64427.1 Different initiation. AE014134 Genomic DNA Translation: AAF52418.3 AY069707 mRNA Translation: AAL39852.1 Different initiation. |
RefSeqi | NP_001285687.1, NM_001298758.1 NP_523493.3, NM_078769.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4AXG | X-ray | 2.80 | C/D | 296-425 | [»] | |
AlphaFoldDBi | Q9VMA3 | |||||
SMRi | Q9VMA3 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 60088, 64 interactors |
ComplexPortali | CPX-3177, eIF4E-cup complex |
DIPi | DIP-17920N |
ELMi | Q9VMA3 |
IntActi | Q9VMA3, 10 interactors |
MINTi | Q9VMA3 |
STRINGi | 7227.FBpp0288761 |
PTM databases
iPTMneti | Q9VMA3 |
Proteomic databases
PaxDbi | Q9VMA3 |
Genome annotation databases
EnsemblMetazoai | FBtr0290322; FBpp0288761; FBgn0000392 FBtr0346452; FBpp0312102; FBgn0000392 |
GeneIDi | 33934 |
KEGGi | dme:Dmel_CG11181 |
UCSCi | CG11181-RB, d. melanogaster |
Organism-specific databases
CTDi | 33934 |
FlyBasei | FBgn0000392, cup |
VEuPathDBi | VectorBase:FBgn0000392 |
Phylogenomic databases
eggNOGi | ENOG502SXKW, Eukaryota |
HOGENOMi | CLU_278765_0_0_1 |
InParanoidi | Q9VMA3 |
OMAi | MGMSNNR |
PhylomeDBi | Q9VMA3 |
Enzyme and pathway databases
SignaLinki | Q9VMA3 |
Miscellaneous databases
BioGRID-ORCSi | 33934, 0 hits in 1 CRISPR screen |
ChiTaRSi | cup, fly |
GenomeRNAii | 33934 |
PROi | PR:Q9VMA3 |
Gene expression databases
Bgeei | FBgn0000392, Expressed in embryo and 21 other tissues |
Genevisiblei | Q9VMA3, DM |
Family and domain databases
DisProti | DP01494 |
IDEALi | IID50295 |
InterProi | View protein in InterPro IPR018862, eIF4E-T |
PANTHERi | PTHR12269, PTHR12269, 2 hits |
Pfami | View protein in Pfam PF10477, EIF4E-T, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CUP_DROME | |
Accessioni | Q9VMA3Primary (citable) accession number: Q9VMA3 Secondary accession number(s): O02432 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 2, 2004 |
Last sequence update: | July 7, 2009 | |
Last modified: | May 25, 2022 | |
This is version 163 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families