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Entry version 135 (18 Sep 2019)
Sequence version 1 (01 May 2000)
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Protein

Protein piwi

Gene

piwi

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Acts via the piwi-interacting RNA (piRNA) metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons (PubMed:26808625, PubMed:15817569, PubMed:17346786). Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements (PubMed:16882972). In ovarian somatic cells, mediates silencing of transposable elements at the transcriptional level in a mael-dependent manner (PubMed:23159368, PubMed:28472469). Involved in silencing of long terminal repeat (LTR) retrotransposons in male germline (PubMed:15817569). In testis, regulates spermatogenesis together with Tudor-SN (PubMed:26808625). In germ cells, mediates silencing at both transcriptional and post-transcriptional levels and is involved in the maintenance of populations of primary and secondary piRNAs. Piwi-mediated transcriptional silencing is accompanied by the formation of His3 'Lys-9' trimethylated (H3K9me3) euchromatin and heterochromatin (PubMed:23434410, PubMed:23392610). In ovary, associates predominantly with antisense piRNAs that contain uridine at their 5' end. Association with sense piRNAs is also observed but to a lesser extent. Mediates a somatic signaling mechanism required for the maintenance of germline stem cells to produce and maintain a daughter germline stem cell (PubMed:9851978, PubMed:10631171, PubMed:9199372, PubMed:16949822). It is not essential for the further differentiation of the committed daughter cell (PubMed:9851978). Acts cell autonomously to promote germline stem cell division (PubMed:9851978, PubMed:10631171). Its role in stem cell maintenance does not seem to require nuclear localization. Required maternally for the posterior localization of osk and vas and for pole cell formation during oogenesis and early embryogenesis (PubMed:16949822). Together with Hop and Hsp83, mediates canalization, also known as developmental robustness, likely via epigenetic silencing of existing genetic variants and suppression of transposon-induced new genetic variation (PubMed:21186352). Shows RNA cleavage activity, although is not required for any of its known functions (PubMed:9199372, PubMed:16882972, PubMed:23297219).19 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the ‘Description’ field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi589MagnesiumBy similarity1
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei614By similarity1
Active sitei685By similarity1
Metal bindingi843Magnesium; via carboxylateBy similarity1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionDevelopmental protein, Endonuclease, Hydrolase, Nuclease, RNA-binding
Biological processRNA-mediated gene silencing
LigandMagnesium, Metal-binding

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Protein piwi (EC:3.1.26.-By similarity)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:piwi
ORF Names:CG6122
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiDrosophila melanogaster (Fruit fly)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri7227 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000803 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 2L

Organism-specific databases

Drosophila genome database

More...
FlyBasei
FBgn0004872 piwi

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

Keywords - Cellular componenti

Chromosome, Cytoplasm, Nucleus

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This subsection of the ‘Pathology and Biotech’ section describes the in vivo effects caused by ablation of the gene (or one or more transcripts) coding for the protein described in the entry. This includes gene knockout and knockdown, provided experiments have been performed in the context of a whole organism or a specific tissue, and not at the single-cell level.<p><a href='/help/disruption_phenotype' target='_top'>More...</a></p>Disruption phenotypei

Female mutants show normal ovarian development up to third instar larval stage (PubMed:9851978, PubMed:9199372, PubMed:26808625). However, adult mutant ovarioles contain germaria lacking germline cells and containing two normal or abnormal egg chambers as result of the failure of germline stem cell maintenance (PubMed:9851978, PubMed:9199372, PubMed:26808625). In adult testis, results in deregulation of transposon silencing (PubMed:26808625).3 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology_and_biotech_section">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi4 – 12Missing : Largely prevents nuclear accumulation. Affects repression activity of soma- and germline-specific transposable elements and fertility. 1 Publication9
Mutagenesisi5Q → A: Reduced binding to papi. 1 Publication1
Mutagenesisi7 – 11RGRRR → AGAAA: Abolishes binding to papi. 1 Publication5
Mutagenesisi7 – 9RGR → KGK: Abolishes binding to papi. 1 Publication3
Mutagenesisi7R → A: Large decrease in binding to papi. 1 Publication1
Mutagenesisi7R → K: Large decrease in binding to papi. 1 Publication1
Mutagenesisi8G → V: Highly significant decrease in binding to papi. 1 Publication1
Mutagenesisi9R → A: Decreased binding to papi. 1 Publication1
Mutagenesisi9R → K: Decreased binding to papi. 1 Publication1
Mutagenesisi10R → A: Abolishes binding to papi. 1 Publication1
Mutagenesisi10R → K: Abolishes binding to papi. 1 Publication1
Mutagenesisi11R → A: Decreased binding to papi. 1 Publication1
Mutagenesisi11R → K: Decreased binding to papi. 1 Publication1
Mutagenesisi12P → A: No significant effect on binding to papi. 1 Publication1
Mutagenesisi30V → A: Abolishes binding to CBX5; when associated with or without A-130. Fails to rescue dominant defects in white reporter silencing produced by the piwi2 mutation. 1 Publication1
Mutagenesisi51 – 54RERR → AEAA: No effect on binding to papi. 1 Publication4
Mutagenesisi54R → G: Confers RNAi insensitivity; when associated with P-67. 1 Publication1
Mutagenesisi61 – 62RR → AA: No effect on binding to papi. 1 Publication2
Mutagenesisi67T → P: Confers RNAi insensitivity; when associated with G-54. 1 Publication1
Mutagenesisi130V → A: Abolishes binding to CBX5; when associated with A-30. 1 Publication1
Mutagenesisi327 – 328YY → AA: Promotes accumulation in the cytoplasm. 1 Publication2
Mutagenesisi551 – 555YSSIK → LSSIE: Abolishes binding to piRNAs. Reduces localization to the nucleus. Does not affect chromatin binding. Affects fertility and ovary morphology. 1 Publication5
Mutagenesisi614D → A: Does not affect nuclear localization, repression activity of soma- and germline-specific transposable elements, fertility and piRNA loading; when associated with or without A-685. 3 Publications1
Mutagenesisi685D → A: Does not affect nuclear localization, repression activity of soma- and germline-specific transposable elements, fertility and piRNA loading; when associated with or without A-614. 3 Publications1

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001940671 – 843Protein piwiAdd BLAST843

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei7Symmetric dimethylarginine1 Publication1
Modified residuei9Symmetric dimethylarginine1 Publication1
Modified residuei10Symmetric dimethylarginine1 Publication1
Modified residuei11Symmetric dimethylarginine1 Publication1

<p>This subsection of the <a href="http://www.uniprot.org/help/ptm_processing_section">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationi

Symmetrically dimethylated, most likely by csul (PubMed:19377467, PubMed:29531043). Methylation at Arg-10 enhances binding to papi whereas methylation at Arg-7, Arg-9 or Arg-11 reduces binding affinity to papi (PubMed:29531043).2 Publications
Phosphorylated on serine and tyrosine residues in an Hsp83-dependent manner.1 Publication

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
Q9VKM1

PRoteomics IDEntifications database

More...
PRIDEi
Q9VKM1

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the ‘Expression’ section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified ‘at protein level’. <br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityi

Expressed in ovaries (at protein level) (PubMed:28472469). Expressed somatically in ovariole terminal filament cells, epithelial sheath cells, cap cells and follicle cells (at protein level). Expressed in nurse cells and oocytes in developing egg chambers (at protein level) (PubMed:29531043, PubMed:29735528). In embryos, accumulates in pole cells (at protein level). In larval and adult testis, expressed in a germinal proliferative center at the apical tip containing somatic hub cells and mitotically dividing germ stem cells (at protein level) (PubMed:26808625).14 Publications

<p>This subsection of the ‘Expression’ section provides information on the expression of the gene product at various stages of a cell, tissue or organism development. By default, the information is derived from experiments at the mRNA level, unless specified ‘at the protein level’.<p><a href='/help/developmental_stage' target='_top'>More...</a></p>Developmental stagei

Expressed both maternally and zygotically. Expressed in the germarium, at low levels during oogenesis stages 1-6, at a lower level during stages 7-9, strongly at stage 10, eventually accumulates in early embryos and later in development the expression decreases (at protein level).5 Publications

Gene expression databases

Bgee dataBase for Gene Expression Evolution

More...
Bgeei
FBgn0004872 Expressed in 9 organ(s), highest expression level in multi-cellular organism

ExpressionAtlas, Differential and Baseline Expression

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ExpressionAtlasi
Q9VKM1 baseline and differential

Genevisible search portal to normalized and curated expression data from Genevestigator

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Genevisiblei
Q9VKM1 DM

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Interacts with vas; this interaction is RNA-independent (PubMed:16949822).

Interacts with Dcr-1 and Fmr1; these interactions occur in polar granules (PubMed:16949822).

Interacts (via N-terminal region) with CBX5 (via chromoshadow domain) (PubMed:17875665).

Forms a complex with Hsp83 and Hop; probably Hop mediates the interaction between piwi and Hsp83 (PubMed:21186352).

Forms a complex with Yb body components armi and fs1Yb; this interaction is required for proper piRNA loading and nuclear localization of piwi (PubMed:20966047). Interaction of Piwi and fs1Yb is likely to occur via armi (PubMed:20966047).

Interacts (via the N-terminal region when unmethylated or symmetrically methylated at Arg-10) with papi (via Tudor domain) (PubMed:21447556, PubMed:29531043).

Interacts with vret (PubMed:21831924).

Interacts with Panx (PubMed:26472911, PubMed:26494711).

Interacts with arx (PubMed:23913921, PubMed:23913922).

Interacts with Tudor-SN (PubMed:26808625).

Interacts with Nup358 (via N-terminus) (PubMed:29735528). Associates with the nuclear pore complex via interaction with Elys (PubMed:28472469).

Interacts with thoc5; the interaction might be partly RNA-mediated (PubMed:28472469).

Interacts with xmas-2 (PubMed:28472469).

14 Publications

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction_section%27">Interaction</a> section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="http://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated on a monthly basis. Each binary interaction is displayed on a separate line.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGrid)

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BioGridi
60588, 42 interactors

Database of interacting proteins

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DIPi
DIP-61694N

Protein interaction database and analysis system

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IntActi
Q9VKM1, 25 interactors

Molecular INTeraction database

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MINTi
Q9VKM1

STRING: functional protein association networks

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STRINGi
7227.FBpp0079755

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

1843
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

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SMRi
Q9VKM1

Database of comparative protein structure models

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ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family_and_domains_section">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini262 – 372PAZPROSITE-ProRule annotationAdd BLAST111
Domaini538 – 829PiwiPROSITE-ProRule annotationAdd BLAST292

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Family and Domains’ section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni1 – 257Interaction with CBX5 and papi1 PublicationAdd BLAST257

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Family and Domains’ section describes a short (usually not more than 20 amino acids) conserved sequence motif of biological significance.<p><a href='/help/motif' target='_top'>More...</a></p>Motifi1 – 12Nuclear localization signalAdd BLAST12

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the argonaute family. Piwi subfamily.Curated

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

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eggNOGi
KOG1042 Eukaryota
ENOG410XNRH LUCA

InParanoid: Eukaryotic Ortholog Groups

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InParanoidi
Q9VKM1

KEGG Orthology (KO)

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KOi
K02156

Identification of Orthologs from Complete Genome Data

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OMAi
LDRWAVI

Database of Orthologous Groups

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OrthoDBi
220258at2759

Database for complete collections of gene phylogenies

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PhylomeDBi
Q9VKM1

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

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Gene3Di
3.30.420.10, 1 hit

Integrated resource of protein families, domains and functional sites

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InterProi
View protein in InterPro
IPR003100 PAZ_dom
IPR036085 PAZ_dom_sf
IPR003165 Piwi
IPR012337 RNaseH-like_sf
IPR036397 RNaseH_sf

Pfam protein domain database

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Pfami
View protein in Pfam
PF02170 PAZ, 1 hit
PF02171 Piwi, 1 hit

Simple Modular Architecture Research Tool; a protein domain database

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SMARTi
View protein in SMART
SM00949 PAZ, 1 hit
SM00950 Piwi, 1 hit

Superfamily database of structural and functional annotation

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SUPFAMi
SSF101690 SSF101690, 1 hit
SSF53098 SSF53098, 1 hit

PROSITE; a protein domain and family database

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PROSITEi
View protein in PROSITE
PS50821 PAZ, 1 hit
PS50822 PIWI, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequence (1+)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q9VKM1-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MADDQGRGRR RPLNEDDSST SRGSGDGPRV KVFRGSSSGD PRADPRIEAS
60 70 80 90 100
RERRALEEAP RREGGPTERK PWGDQYDYLN TRPAELVSKK GTDGVPVMLQ
110 120 130 140 150
TNFFRLKTKP EWRIVHYHVE FEPSIENPRV RMGVLSNHAN LLGSGYLFDG
160 170 180 190 200
LQLFTTRKFE QEITVLSGKS KLDIEYKISI KFVGFISCAE PRFLQVLNLI
210 220 230 240 250
LRRSMKGLNL ELVGRNLFDP RAKIEIREFK MELWPGYETS IRQHEKDILL
260 270 280 290 300
GTEITHKVMR TETIYDIMRR CSHNPARHQD EVRVNVLDLI VLTDYNNRTY
310 320 330 340 350
RINDVDFGQT PKSTFSCKGR DISFVEYYLT KYNIRIRDHN QPLLISKNRD
360 370 380 390 400
KALKTNASEL VVLIPELCRV TGLNAEMRSN FQLMRAMSSY TRMNPKQRTD
410 420 430 440 450
RLRAFNHRLQ NTPESVKVLR DWNMELDKNV TEVQGRIIGQ QNIVFHNGKV
460 470 480 490 500
PAGENADWQR HFRDQRMLTT PSDGLDRWAV IAPQRNSHEL RTLLDSLYRA
510 520 530 540 550
ASGMGLRIRS PQEFIIYDDR TGTYVRAMDD CVRSDPKLIL CLVPNDNAER
560 570 580 590 600
YSSIKKRGYV DRAVPTQVVT LKTTKNRSLM SIATKIAIQL NCKLGYTPWM
610 620 630 640 650
IELPLSGLMT IGFDIAKSTR DRKRAYGALI ASMDLQQNST YFSTVTECSA
660 670 680 690 700
FDVLANTLWP MIAKALRQYQ HEHRKLPSRI VFYRDGVSSG SLKQLFEFEV
710 720 730 740 750
KDIIEKLKTE YARVQLSPPQ LAYIVVTRSM NTRFFLNGQN PPPGTIVDDV
760 770 780 790 800
ITLPERYDFY LVSQQVRQGT VSPTSYNVLY SSMGLSPEKM QKLTYKMCHL
810 820 830 840
YYNWSGTTRV PAVCQYAKKL ATLVGTNLHS IPQNALEKKF YYL
Length:843
Mass (Da):97,177
Last modified:May 1, 2000 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i9B4A95E688E9D9B7
GO

<p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
X2J7U0X2J7U0_DROME
P-element induced wimpy testis, iso...
piwi Dmel\CG6122, PIWI, Piwi, CG6122, Dmel_CG6122
843Annotation score:

Annotation score:2 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

The sequence AAR82763 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence AAR82805 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence ACN43727 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Sequence’ section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti33F → S in AFX62841 (Ref. 7) Curated1
Sequence conflicti33F → S in AFX62842 (Ref. 7) Curated1
Sequence conflicti54R → G in AGA18878 (PubMed:22997235).Curated1
Sequence conflicti54R → G in AGA18879 (PubMed:22997235).Curated1
Sequence conflicti54R → G in AGA18881 (PubMed:22997235).Curated1
Sequence conflicti54R → G in AGA18882 (PubMed:22997235).Curated1
Sequence conflicti54R → G in AGA18883 (PubMed:22997235).Curated1
Sequence conflicti54R → G in AGA18884 (PubMed:22997235).Curated1
Sequence conflicti54R → G in AAR82805 (Ref. 5) Curated1
Sequence conflicti54R → G in AFX62837 (Ref. 7) Curated1
Sequence conflicti54R → G in AFX62838 (Ref. 7) Curated1
Sequence conflicti54R → G in AFX62839 (Ref. 7) Curated1
Sequence conflicti54R → G in AFX62841 (Ref. 7) Curated1
Sequence conflicti54R → G in AFX62842 (Ref. 7) Curated1
Sequence conflicti67T → P in AAD08704 (PubMed:9851978).Curated1
Sequence conflicti67T → P in AGA18878 (PubMed:22997235).Curated1
Sequence conflicti67T → P in AGA18879 (PubMed:22997235).Curated1
Sequence conflicti67T → P in AGA18880 (PubMed:22997235).Curated1
Sequence conflicti67T → P in AGA18881 (PubMed:22997235).Curated1
Sequence conflicti67T → P in AGA18882 (PubMed:22997235).Curated1
Sequence conflicti67T → P in AGA18883 (PubMed:22997235).Curated1
Sequence conflicti67T → P in AGA18884 (PubMed:22997235).Curated1
Sequence conflicti67T → P in AAR82805 (Ref. 5) Curated1
Sequence conflicti67T → P in AFX62837 (Ref. 7) Curated1
Sequence conflicti67T → P in AFX62838 (Ref. 7) Curated1
Sequence conflicti67T → P in AFX62839 (Ref. 7) Curated1
Sequence conflicti67T → P in AFX62841 (Ref. 7) Curated1
Sequence conflicti67T → P in AFX62842 (Ref. 7) Curated1
Sequence conflicti80N → D in AGA18882 (PubMed:22997235).Curated1
Sequence conflicti80N → D in AGA18883 (PubMed:22997235).Curated1
Sequence conflicti80N → D in AAR82805 (Ref. 5) Curated1
Sequence conflicti84A → V in AAD08704 (PubMed:9851978).Curated1
Sequence conflicti84A → V in AGA18878 (PubMed:22997235).Curated1
Sequence conflicti84A → V in AGA18879 (PubMed:22997235).Curated1
Sequence conflicti84A → V in AGA18880 (PubMed:22997235).Curated1
Sequence conflicti84A → V in AGA18881 (PubMed:22997235).Curated1
Sequence conflicti84A → V in AGA18882 (PubMed:22997235).Curated1
Sequence conflicti84A → V in AGA18883 (PubMed:22997235).Curated1
Sequence conflicti84A → V in AGA18884 (PubMed:22997235).Curated1
Sequence conflicti84A → V in AAR82805 (Ref. 5) Curated1
Sequence conflicti84A → V in AFX62837 (Ref. 7) Curated1
Sequence conflicti84A → V in AFX62838 (Ref. 7) Curated1
Sequence conflicti84A → V in AFX62839 (Ref. 7) Curated1
Sequence conflicti84A → V in AFX62841 (Ref. 7) Curated1
Sequence conflicti84A → V in AFX62842 (Ref. 7) Curated1
Sequence conflicti122 – 124EPS → VPT in AAD08704 (PubMed:9851978).Curated3
Sequence conflicti122 – 124EPS → VPT in AGA18880 (PubMed:22997235).Curated3
Sequence conflicti122 – 124EPS → VPT in AGA18881 (PubMed:22997235).Curated3
Sequence conflicti122 – 124EPS → VPT in AGA18882 (PubMed:22997235).Curated3
Sequence conflicti122 – 124EPS → VPT in AGA18883 (PubMed:22997235).Curated3
Sequence conflicti122 – 124EPS → VPT in AAR82805 (Ref. 5) Curated3
Sequence conflicti182F → I in AGA18884 (PubMed:22997235).Curated1
Sequence conflicti193F → L in AFX62841 (Ref. 7) Curated1
Sequence conflicti226I → V in AFX62841 (Ref. 7) Curated1
Sequence conflicti240S → L in AFX62842 (Ref. 7) Curated1
Sequence conflicti325V → L in AAR82805 (Ref. 5) Curated1
Sequence conflicti364I → V in AFX62837 (Ref. 7) Curated1
Sequence conflicti364I → V in AFX62838 (Ref. 7) Curated1
Sequence conflicti496S → P in AFX62837 (Ref. 7) Curated1
Sequence conflicti496S → P in AFX62838 (Ref. 7) Curated1
Sequence conflicti506L → P in AFX62837 (Ref. 7) Curated1
Sequence conflicti506L → P in AFX62838 (Ref. 7) Curated1
Sequence conflicti556K → R in AFX62839 (Ref. 7) Curated1
Sequence conflicti576 – 577NR → KPY in AAD08705 (PubMed:9851978).Curated2
Sequence conflicti686G → R in AAR82763 (Ref. 5) Curated1
Sequence conflicti728R → G in AFX62839 (Ref. 7) Curated1
Sequence conflicti818K → E in AFX62842 (Ref. 7) Curated1

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

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EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

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DDBJi
Links Updated
AF104354 mRNA Translation: AAD08704.1
AF104355 Genomic DNA Translation: AAD08705.1
KC116149 Genomic DNA Translation: AGA18878.1
KC116150 Genomic DNA Translation: AGA18879.1
KC116151 Genomic DNA Translation: AGA18880.1
KC116152 Genomic DNA Translation: AGA18881.1
KC116153 Genomic DNA Translation: AGA18882.1
KC116154 Genomic DNA Translation: AGA18883.1
KC116155 Genomic DNA Translation: AGA18884.1
AE014134 Genomic DNA Translation: AAF53043.1
BT011097 mRNA Translation: AAR82763.1 Different initiation.
BT011138 mRNA Translation: AAR82805.1 Different initiation.
BT071791 mRNA Translation: ACN43727.1 Different initiation.
JX656897 mRNA Translation: AFX62837.1
JX656898 mRNA Translation: AFX62838.1
JX656899 mRNA Translation: AFX62839.1
JX656901 mRNA Translation: AFX62841.1
JX656902 mRNA Translation: AFX62842.1

NCBI Reference Sequences

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RefSeqi
NP_001285825.1, NM_001298896.1
NP_476875.1, NM_057527.4

Genome annotation databases

Ensembl metazoan genome annotation project

More...
EnsemblMetazoai
FBtr0080166; FBpp0079755; FBgn0004872
FBtr0340227; FBpp0309202; FBgn0004872

Database of genes from NCBI RefSeq genomes

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GeneIDi
34521

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
dme:Dmel_CG6122

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF104354 mRNA Translation: AAD08704.1
AF104355 Genomic DNA Translation: AAD08705.1
KC116149 Genomic DNA Translation: AGA18878.1
KC116150 Genomic DNA Translation: AGA18879.1
KC116151 Genomic DNA Translation: AGA18880.1
KC116152 Genomic DNA Translation: AGA18881.1
KC116153 Genomic DNA Translation: AGA18882.1
KC116154 Genomic DNA Translation: AGA18883.1
KC116155 Genomic DNA Translation: AGA18884.1
AE014134 Genomic DNA Translation: AAF53043.1
BT011097 mRNA Translation: AAR82763.1 Different initiation.
BT011138 mRNA Translation: AAR82805.1 Different initiation.
BT071791 mRNA Translation: ACN43727.1 Different initiation.
JX656897 mRNA Translation: AFX62837.1
JX656898 mRNA Translation: AFX62838.1
JX656899 mRNA Translation: AFX62839.1
JX656901 mRNA Translation: AFX62841.1
JX656902 mRNA Translation: AFX62842.1
RefSeqiNP_001285825.1, NM_001298896.1
NP_476875.1, NM_057527.4

3D structure databases

Select the link destinations:

Protein Data Bank Europe

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PDBei

Protein Data Bank RCSB

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RCSB PDBi

Protein Data Bank Japan

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PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5YGDX-ray1.55D4-14[»]
5YGFX-ray1.70D4-12[»]
SMRiQ9VKM1
ModBaseiSearch...

Protein-protein interaction databases

BioGridi60588, 42 interactors
DIPiDIP-61694N
IntActiQ9VKM1, 25 interactors
MINTiQ9VKM1
STRINGi7227.FBpp0079755

Proteomic databases

PaxDbiQ9VKM1
PRIDEiQ9VKM1

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0080166; FBpp0079755; FBgn0004872
FBtr0340227; FBpp0309202; FBgn0004872
GeneIDi34521
KEGGidme:Dmel_CG6122

Organism-specific databases

Comparative Toxicogenomics Database

More...
CTDi
34521
FlyBaseiFBgn0004872 piwi

Phylogenomic databases

eggNOGiKOG1042 Eukaryota
ENOG410XNRH LUCA
InParanoidiQ9VKM1
KOiK02156
OMAiLDRWAVI
OrthoDBi220258at2759
PhylomeDBiQ9VKM1

Miscellaneous databases

Database of phenotypes from RNA interference screens in Drosophila and Homo sapiens

More...
GenomeRNAii
34521

Protein Ontology

More...
PROi
PR:Q9VKM1

Gene expression databases

BgeeiFBgn0004872 Expressed in 9 organ(s), highest expression level in multi-cellular organism
ExpressionAtlasiQ9VKM1 baseline and differential
GenevisibleiQ9VKM1 DM

Family and domain databases

Gene3Di3.30.420.10, 1 hit
InterProiView protein in InterPro
IPR003100 PAZ_dom
IPR036085 PAZ_dom_sf
IPR003165 Piwi
IPR012337 RNaseH-like_sf
IPR036397 RNaseH_sf
PfamiView protein in Pfam
PF02170 PAZ, 1 hit
PF02171 Piwi, 1 hit
SMARTiView protein in SMART
SM00949 PAZ, 1 hit
SM00950 Piwi, 1 hit
SUPFAMiSSF101690 SSF101690, 1 hit
SSF53098 SSF53098, 1 hit
PROSITEiView protein in PROSITE
PS50821 PAZ, 1 hit
PS50822 PIWI, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

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ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiPIWI_DROME
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q9VKM1
Secondary accession number(s): C0PTU6
, K7WKS7, K7WKT2, K7WQ39, K7WS94, K7XHZ2, L0CPR8, L0CPS4, L0CQ04, L0CR36, L0CRH9, L0CRI5, L0CRU0, O96674, O96675, Q6NNZ4, Q6NP34
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2003
Last sequence update: May 1, 2000
Last modified: September 18, 2019
This is version 135 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
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