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Protein

Mitochondrial cardiolipin hydrolase

Gene

zuc

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cardiolipin hydrolase present at the mitochondrial outer membrane required for piRNA metabolic process. Acts by catalyzing the hydrolysis of cardiolipin (diphosphatidylglycerol) to form phosphatidate (phosphatidic acid or PA) at the mitochondrial outer membrane surface, promoting the piRNA metabolic process. Plays a key role in primary biogenesis of piRNAs and is required during oogenesis to repress transposable elements and prevent their mobilization. piRNAs mediate the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Involved in trans-silencing effect (TSE), a homology-dependent repression mechanism by which a P-transgene inserted in subtelomeric heterochromatin via its role in piRNA biogenesis.7 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei169Curated1
Active sitei171Sequence analysis1
Active sitei176Sequence analysis1

GO - Molecular functioni

  • cardiolipin hydrolase activity Source: UniProtKB
  • endoribonuclease activity, producing 5'-phosphomonoesters Source: FlyBase
  • identical protein binding Source: IntAct

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processDifferentiation, Lipid degradation, Lipid metabolism, Meiosis, Oogenesis

Enzyme and pathway databases

ReactomeiR-DME-1483166 Synthesis of PA

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial cardiolipin hydrolase (EC:3.1.4.-)
Alternative name(s):
Mitochondrial phospholipaseBy similarity
Short name:
MitoPLDBy similarity
Protein zucchini1 Publication
Gene namesi
Name:zuc1 PublicationImported
ORF Names:CG12314Imported
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0261266 zuc

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 13Mitochondrial intermembraneSequence analysisAdd BLAST13
Transmembranei14 – 30HelicalSequence analysisAdd BLAST17
Topological domaini31 – 253CytoplasmicSequence analysisAdd BLAST223

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion outer membrane

Pathology & Biotechi

Disruption phenotypei

Defects in mid oogenesis. Females are viable but produce eggs with a range of dorso-ventral patterning defects. Flies lay few eggs, all of which are completely ventralized and often collapsed. Effects are due to defects in piRNA biogenesis and derepression of retrotransposons. Defects are not only present in germ cells but also in somatic cells of the ovary.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi47A → D in zuc(RS49); produce some eggs with a more normal eggshell phenotype in addition to the ventralized eggs compared to null mutants. 1 Publication1
Mutagenesisi169H → N: Abolishes cardiolipin hydrolase activity. 2 Publications1
Mutagenesisi169H → Y in zuc(SG63); produce some eggs with a more normal eggshell phenotype in addition to the ventralized eggs compared to null mutants. 2 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004083351 – 253Mitochondrial cardiolipin hydrolaseAdd BLAST253

Proteomic databases

PaxDbiQ9VKD7
PRIDEiQ9VKD7

Expressioni

Gene expression databases

BgeeiFBgn0261266 Expressed in 25 organ(s), highest expression level in embryo
ExpressionAtlasiQ9VKD7 differential
GenevisibleiQ9VKD7 DM

Interactioni

Subunit structurei

Homodimer (By similarity). Interacts with miga/CG12125 (PubMed:26711011).By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-9943000,EBI-9943000

GO - Molecular functioni

Protein-protein interaction databases

BioGridi60662, 6 interactors
DIPiDIP-59983N
IntActiQ9VKD7, 1 interactor
STRINGi7227.FBpp0079900

Structurei

Secondary structure

1253
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9VKD7
SMRiQ9VKD7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini164 – 211PLD phosphodiesteraseAdd BLAST48

Domaini

In contrast to other members of the phospholipase D family, contains only one PLD phosphodiesterase domain, suggesting that it has a single half-catalytic and requires homodimerization to form a complete active site.By similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410J0H4 Eukaryota
COG1502 LUCA
GeneTreeiENSGT00390000004368
InParanoidiQ9VKD7
KOiK16862
OMAiASEVIWK
OrthoDBiEOG091G0FI5
PhylomeDBiQ9VKD7

Family and domain databases

InterProiView protein in InterPro
IPR025202 PLD-like_dom
PfamiView protein in Pfam
PF13091 PLDc_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q9VKD7-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MLITQIIMKQ IRDYPIVSTI SIAVSTVLAS EVIWKLVQCS RSKREKASRV
60 70 80 90 100
HEVIIFNELG EICAAVHMRN SSMGSQKPQV SPCCNTHCSL RNVAKIVEQI
110 120 130 140 150
DRAVYSIDLA IYTFTSLFLA DSIKRALQRG VIIRIISDGE MVYSKGSQIS
160 170 180 190 200
MLAQLGVPVR VPITTNLMHN KFCIIDGFER VEEIRLLRKL KFMRPCYSIV
210 220 230 240 250
ISGSVNWTAL GLGGNWENCI ITADDKLTAT FQAEFQRMWR AFAKTEGSQI

QLK
Length:253
Mass (Da):28,520
Last modified:May 1, 2000 - v1
Checksum:i4488BEA6DFF9E092
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti2L → V in strain: MEL20, ZBMEL131, ZBMEL186, ZBMEL384 and ZBMEL398. 1
Natural varianti225D → E in strain: =MEL01, MEL02, MEL11, MEL12, MEL14, MEL15, MEL16, MEL17, MEL18, MEL19, MEL20, ZBMEL82, ZBMEL95, ZBMEL131, ZBMEL145, ZBMEL157, ZBMEL186, ZBMEL191, ZBMEL229, ZBMEL377, ZBMEL384 and ZBMEL398. 1
Natural varianti252L → V in strain: ZBMEL191, ZBMEL229, ZBMEL377 and ZBMEL384. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM294430 Genomic DNA Translation: CAL26369.1
AM294431 Genomic DNA Translation: CAL26370.1
AM294432 Genomic DNA Translation: CAL26371.1
AM294433 Genomic DNA Translation: CAL26372.1
AM294434 Genomic DNA Translation: CAL26373.1
AM294435 Genomic DNA Translation: CAL26374.1
AM294436 Genomic DNA Translation: CAL26375.1
AM294437 Genomic DNA Translation: CAL26377.1
AM294438 Genomic DNA Translation: CAL26381.1
AM294439 Genomic DNA Translation: CAL26382.1
AM294440 Genomic DNA Translation: CAL26383.1
FM245502 Genomic DNA Translation: CAR93428.1
FM245503 Genomic DNA Translation: CAR93429.1
FM245504 Genomic DNA Translation: CAR93430.1
FM245505 Genomic DNA Translation: CAR93431.1
FM245506 Genomic DNA Translation: CAR93432.1
FM245507 Genomic DNA Translation: CAR93433.1
FM245508 Genomic DNA Translation: CAR93434.1
FM245509 Genomic DNA Translation: CAR93435.1
FM245510 Genomic DNA Translation: CAR93436.1
FM245511 Genomic DNA Translation: CAR93437.1
FM245512 Genomic DNA Translation: CAR93438.1
FM245513 Genomic DNA Translation: CAR93439.1
AE014134 Genomic DNA Translation: AAF53139.1
AY118493 mRNA Translation: AAM49862.1
RefSeqiNP_609530.1, NM_135686.3
UniGeneiDm.11087

Genome annotation databases

EnsemblMetazoaiFBtr0080316; FBpp0079900; FBgn0261266
GeneIDi34609
KEGGidme:Dmel_CG12314
UCSCiCG12314-RA d. melanogaster

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM294430 Genomic DNA Translation: CAL26369.1
AM294431 Genomic DNA Translation: CAL26370.1
AM294432 Genomic DNA Translation: CAL26371.1
AM294433 Genomic DNA Translation: CAL26372.1
AM294434 Genomic DNA Translation: CAL26373.1
AM294435 Genomic DNA Translation: CAL26374.1
AM294436 Genomic DNA Translation: CAL26375.1
AM294437 Genomic DNA Translation: CAL26377.1
AM294438 Genomic DNA Translation: CAL26381.1
AM294439 Genomic DNA Translation: CAL26382.1
AM294440 Genomic DNA Translation: CAL26383.1
FM245502 Genomic DNA Translation: CAR93428.1
FM245503 Genomic DNA Translation: CAR93429.1
FM245504 Genomic DNA Translation: CAR93430.1
FM245505 Genomic DNA Translation: CAR93431.1
FM245506 Genomic DNA Translation: CAR93432.1
FM245507 Genomic DNA Translation: CAR93433.1
FM245508 Genomic DNA Translation: CAR93434.1
FM245509 Genomic DNA Translation: CAR93435.1
FM245510 Genomic DNA Translation: CAR93436.1
FM245511 Genomic DNA Translation: CAR93437.1
FM245512 Genomic DNA Translation: CAR93438.1
FM245513 Genomic DNA Translation: CAR93439.1
AE014134 Genomic DNA Translation: AAF53139.1
AY118493 mRNA Translation: AAM49862.1
RefSeqiNP_609530.1, NM_135686.3
UniGeneiDm.11087

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4GELX-ray1.76A/B41-253[»]
4GEMX-ray2.21A/B41-253[»]
4GENX-ray2.20A89-250[»]
4H4AX-ray2.20A89-253[»]
ProteinModelPortaliQ9VKD7
SMRiQ9VKD7
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi60662, 6 interactors
DIPiDIP-59983N
IntActiQ9VKD7, 1 interactor
STRINGi7227.FBpp0079900

Proteomic databases

PaxDbiQ9VKD7
PRIDEiQ9VKD7

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0080316; FBpp0079900; FBgn0261266
GeneIDi34609
KEGGidme:Dmel_CG12314
UCSCiCG12314-RA d. melanogaster

Organism-specific databases

CTDi34609
FlyBaseiFBgn0261266 zuc

Phylogenomic databases

eggNOGiENOG410J0H4 Eukaryota
COG1502 LUCA
GeneTreeiENSGT00390000004368
InParanoidiQ9VKD7
KOiK16862
OMAiASEVIWK
OrthoDBiEOG091G0FI5
PhylomeDBiQ9VKD7

Enzyme and pathway databases

ReactomeiR-DME-1483166 Synthesis of PA

Miscellaneous databases

GenomeRNAii34609
PROiPR:Q9VKD7

Gene expression databases

BgeeiFBgn0261266 Expressed in 25 organ(s), highest expression level in embryo
ExpressionAtlasiQ9VKD7 differential
GenevisibleiQ9VKD7 DM

Family and domain databases

InterProiView protein in InterPro
IPR025202 PLD-like_dom
PfamiView protein in Pfam
PF13091 PLDc_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiZUC_DROME
AccessioniPrimary (citable) accession number: Q9VKD7
Secondary accession number(s): A0AP42
, A0AP43, A0AP44, A0AP47, A0AP48, A0AP51
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 3, 2011
Last sequence update: May 1, 2000
Last modified: November 7, 2018
This is version 114 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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