UniProtKB - Q9VIF5 (DEL_DROME)
Protein deadlock
del
Functioni
Developmental protein involved in oogenesis (PubMed:16616913).
Required for germline maintenance, stability of mitotic spindles, localization of patterning determinants, oocyte growth and fusome biogenesis in males and females (PubMed:16616913).
Also required for dorso-ventral and antero-posterior patterning of oocyte and eggshell (PubMed:16616913).
May be involved in microtubule function during oogenesis (PubMed:16616913).
Part of a rhi-dependent transcription machinery that enables the generation of piRNA precursors from heterochromatin while maintaining the suppression of transposon-encoded promoters and enhancers (PubMed:28847004).
Component of the RDC complex (rhi, del and cuff) which binds to repressive H3K9me3 marks in the piRNA clusters (PubMed:28847004).
RDC promotes the bidirectional transcription of piRNA clusters at these sites by interacting with Moonshiner which forms a complex with the transcription initiation factors TfIIA-S and Trf2 (PubMed:28847004).
This mechanism allows transcription to occur in piRNA clusters despite the lack of proper promoter elements and in the presence of the repressive H3K9me3 mark (PubMed:28847004).
2 PublicationsGO - Biological processi
- fusome organization Source: UniProtKB
- germarium-derived egg chamber formation Source: FlyBase
- mitotic spindle organization Source: UniProtKB
- oocyte development Source: UniProtKB
- oocyte karyosome formation Source: FlyBase
- oocyte localization involved in germarium-derived egg chamber formation Source: FlyBase
- oogenesis Source: UniProtKB
- piRNA metabolic process Source: FlyBase
Keywordsi
Molecular function | Developmental protein |
Biological process | Differentiation, Oogenesis, Transcription, Transcription regulation |
Enzyme and pathway databases
SignaLinki | Q9VIF5 |
Names & Taxonomyi
Protein namesi | Recommended name: Protein deadlock |
Gene namesi | Name:del ORF Names:CG9252 |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0086251, del |
VEuPathDBi | VectorBase:FBgn0086251 |
Subcellular locationi
Nucleus
- Nucleus 2 Publications
Cytoskeleton
- centrosome 1 Publication
Note: Colocalizes with the oocyte nucleus at midstages of oogenesis and with the centrosomes of early embryos (PubMed:16616913). Expressed in nuclear foci (PubMed:28847004).2 Publications
Cytoskeleton
- centrosome Source: UniProtKB
Nucleus
- nucleus Source: UniProtKB
Other locations
- cytoplasm Source: UniProtKB-KW
- heterochromatin Source: FlyBase
Keywords - Cellular componenti
Cytoplasm, Cytoskeleton, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000235296 | 1 – 981 | Protein deadlockAdd BLAST | 981 |
Proteomic databases
PaxDbi | Q9VIF5 |
Expressioni
Gene expression databases
Bgeei | FBgn0086251, Expressed in female gonad and 30 other tissues |
ExpressionAtlasi | Q9VIF5, baseline and differential |
Genevisiblei | Q9VIF5, DM |
Interactioni
Binary interactionsi
Q9VIF5
With | #Exp. | IntAct |
---|---|---|
cuff [Q9V629] | 4 | EBI-151790,EBI-185996 |
Protein-protein interaction databases
BioGRIDi | 61335, 8 interactors |
ComplexPortali | CPX-3213, Rhino-Deadlock-Cutoff Complex |
IntActi | Q9VIF5, 6 interactors |
STRINGi | 7227.FBpp0305298 |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | Q9VIF5 |
SMRi | Q9VIF5 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 72 – 195 | DisorderedSequence analysisAdd BLAST | 124 | |
Regioni | 274 – 307 | DisorderedSequence analysisAdd BLAST | 34 | |
Regioni | 327 – 352 | DisorderedSequence analysisAdd BLAST | 26 | |
Regioni | 375 – 446 | DisorderedSequence analysisAdd BLAST | 72 | |
Regioni | 554 – 586 | DisorderedSequence analysisAdd BLAST | 33 | |
Regioni | 642 – 662 | DisorderedSequence analysisAdd BLAST | 21 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 98 – 159 | Polar residuesSequence analysisAdd BLAST | 62 | |
Compositional biasi | 160 – 192 | Basic and acidic residuesSequence analysisAdd BLAST | 33 | |
Compositional biasi | 333 – 351 | Basic and acidic residuesSequence analysisAdd BLAST | 19 | |
Compositional biasi | 398 – 413 | Polar residuesSequence analysisAdd BLAST | 16 | |
Compositional biasi | 419 – 446 | Polar residuesSequence analysisAdd BLAST | 28 | |
Compositional biasi | 559 – 574 | Basic and acidic residuesSequence analysisAdd BLAST | 16 |
Phylogenomic databases
eggNOGi | ENOG502T86W, Eukaryota |
InParanoidi | Q9VIF5 |
OrthoDBi | 482084at2759 |
PhylomeDBi | Q9VIF5 |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MEKLDKIRMS QKLSCWQHIL TTLGTSSKTE QEWNTFFKGF LESWRKPYCI
60 70 80 90 100
QTSCDPSIPL RKELLVRPRK ALQENPHGPG STLPESPVFL EPINSTAPRE
110 120 130 140 150
HPSPSKSHST GSTGCDPGNG ERSPSVSNKN SKYKNPGNSK YAKIWKRSNN
160 170 180 190 200
HTTSIFSKAQ ISKRRDKLSS TKKRPDTCAP TDDSRKNREP RACAPNKNIF
210 220 230 240 250
KTRETNAPNL TKNSCALPNV LILSPNSASK ITQRGHSVGQ TQDYKASPGK
260 270 280 290 300
IIKRVPRYSL QCLKKKTEKV HNKIMDKPKN KQQPQTPPPF LLNNEYTESS
310 320 330 340 350
DDSDDQLPLS ELSQKMKSNK LNTLFLSRNE DCSPAPEKVK LKGERPAQNK
360 370 380 390 400
KEQLTWEPSI LTNLTDLGKQ VAEPLRKSVK KSAKQQKPRV RAPPQGKKTL
410 420 430 440 450
PQLQTGLKTQ DKQSTHEMIS EQAKTISEAS GQQTSQVQSS LSPNNIRNNS
460 470 480 490 500
VKLISAKTLM PAQRSQDYSP NKMQVGQPLG FAIELPSPVD GKEASVEIDT
510 520 530 540 550
QLVHTSKFLE NMTRPSTVEV HKFDLMDIFM GENEKLDYEY DDDDVLSVAA
560 570 580 590 600
SWNGLDDENV PEDEPRKEAK TAEQLPKPEP STETLKPIEK ENAQKMQSIK
610 620 630 640 650
SFQIPKLNAK NLKTQPSVMR SIYENEELEK NKVLAKPAPP SLVHQPLAES
660 670 680 690 700
NRNQRDEATA ARRAKETFPV FAPLYRVAPE SAATLVSANS QQVIPQVYFS
710 720 730 740 750
TSNQDGVNWI KDVFGIRCMQ SVDNKCISIN CDHTMNSLGE VQKRLMRMDE
760 770 780 790 800
DTLLSLYRQT IRSFFLFQTY YTSFVDIFKF RNLWQYLLIM LVDCRLYKSI
810 820 830 840 850
SAPLLAHVYE ALSKCGMQKE AVKRIMEHVW LPCKAHKYRD LMLTTLNILS
860 870 880 890 900
NANWEDYCDK LTQLDKDYNF EIPHKNLITI LKSSVDCSDK FANALKLITL
910 920 930 940 950
HPNSIRTNET IMSILSNASK SYSYMHNESA SASQGPPGAA SFLAPPAAIQ
960 970 980
PPHTTVPNFG YLPNPSFHYS NEYAINIHNF D
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketM9PDK1 | M9PDK1_DROME | Deadlock, isoform C | del Del, Dmel\CG9252, CG9252, Dmel_CG9252 | 981 | Annotation score: | ||
M9PDW9 | M9PDW9_DROME | Deadlock, isoform B | del Del, Dmel\CG9252, CG9252, Dmel_CG9252 | 978 | Annotation score: |
Sequence cautioni
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE014134 Genomic DNA Translation: AAF53964.2 BT003434 mRNA Translation: AAO39437.1 Different initiation. |
RefSeqi | NP_001260666.1, NM_001273737.1 NP_610091.2, NM_136247.3 |
Genome annotation databases
EnsemblMetazoai | FBtr0081502; FBpp0081030; FBgn0086251 FBtr0333085; FBpp0305298; FBgn0086251 |
GeneIDi | 35380 |
KEGGi | dme:Dmel_CG9252 |
UCSCi | CG9252-RA, d. melanogaster |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE014134 Genomic DNA Translation: AAF53964.2 BT003434 mRNA Translation: AAO39437.1 Different initiation. |
RefSeqi | NP_001260666.1, NM_001273737.1 NP_610091.2, NM_136247.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5XYV | X-ray | 2.10 | C/D | 1-60 | [»] | |
AlphaFoldDBi | Q9VIF5 | |||||
SMRi | Q9VIF5 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 61335, 8 interactors |
ComplexPortali | CPX-3213, Rhino-Deadlock-Cutoff Complex |
IntActi | Q9VIF5, 6 interactors |
STRINGi | 7227.FBpp0305298 |
Proteomic databases
PaxDbi | Q9VIF5 |
Protocols and materials databases
DNASUi | 35380 |
Genome annotation databases
EnsemblMetazoai | FBtr0081502; FBpp0081030; FBgn0086251 FBtr0333085; FBpp0305298; FBgn0086251 |
GeneIDi | 35380 |
KEGGi | dme:Dmel_CG9252 |
UCSCi | CG9252-RA, d. melanogaster |
Organism-specific databases
CTDi | 35380 |
FlyBasei | FBgn0086251, del |
VEuPathDBi | VectorBase:FBgn0086251 |
Phylogenomic databases
eggNOGi | ENOG502T86W, Eukaryota |
InParanoidi | Q9VIF5 |
OrthoDBi | 482084at2759 |
PhylomeDBi | Q9VIF5 |
Enzyme and pathway databases
SignaLinki | Q9VIF5 |
Miscellaneous databases
BioGRID-ORCSi | 35380, 0 hits in 1 CRISPR screen |
GenomeRNAii | 35380 |
PROi | PR:Q9VIF5 |
Gene expression databases
Bgeei | FBgn0086251, Expressed in female gonad and 30 other tissues |
ExpressionAtlasi | Q9VIF5, baseline and differential |
Genevisiblei | Q9VIF5, DM |
Family and domain databases
MobiDBi | Search... |
Entry informationi
Entry namei | DEL_DROME | |
Accessioni | Q9VIF5Primary (citable) accession number: Q9VIF5 Secondary accession number(s): Q86P81 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 16, 2006 |
Last sequence update: | October 1, 2002 | |
Last modified: | May 25, 2022 | |
This is version 133 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - PDB cross-references
Index of Protein Data Bank (PDB) cross-references