We will be switching to the new UniProt website soon. Please explore and share your feedback.
Take me to the new website.
UniProtKB - Q9VI75 (PICAL_DROME)
Protein
Phosphatidylinositol-binding clathrin assembly protein LAP
Gene
lap
Organism
Drosophila melanogaster (Fruit fly)
Status
Functioni
Assembly protein recruiting clathrin and adaptor protein complex 2 (AP2) to cell membranes at sites of coated-pit formation and clathrin-vesicle assembly. May be required to determine the amount of membrane to be recycled, possibly by regulating the size of the clathrin cage. Involved in AP2-dependent clathrin-mediated endocytosis at the neuromuscular junction.
1 PublicationGO - Molecular functioni
- 1-phosphatidylinositol binding Source: UniProtKB
- clathrin binding Source: UniProtKB
- clathrin heavy chain binding Source: GO_Central
- phosphatidylinositol-4,5-bisphosphate binding Source: GO_Central
- SNARE binding Source: GO_Central
GO - Biological processi
- chemical synaptic transmission Source: FlyBase
- clathrin coat assembly Source: InterPro
- clathrin-dependent endocytosis Source: GO_Central
- clathrin-dependent synaptic vesicle endocytosis Source: FlyBase
- neuron-neuron synaptic transmission Source: FlyBase
- phototaxis Source: FlyBase
- positive regulation of clathrin-dependent endocytosis Source: FlyBase
- receptor-mediated endocytosis Source: UniProtKB
- synaptic vesicle budding from presynaptic endocytic zone membrane Source: GO_Central
- synaptic vesicle transport Source: FlyBase
- vesicle budding from membrane Source: GO_Central
Keywordsi
Biological process | Endocytosis |
Enzyme and pathway databases
Reactomei | R-DME-432722, Golgi Associated Vesicle Biogenesis R-DME-8856825, Cargo recognition for clathrin-mediated endocytosis R-DME-8856828, Clathrin-mediated endocytosis |
Names & Taxonomyi
Protein namesi | Recommended name: Phosphatidylinositol-binding clathrin assembly protein LAPAlternative name(s): Like-AP180 |
Gene namesi | Name:lap ORF Names:CG2520 |
Organismi | Drosophila melanogaster (Fruit fly)Imported |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0086372, lap |
VEuPathDBi | VectorBase:FBgn0086372 |
Subcellular locationi
Golgi apparatus
- Golgi apparatus 1 Publication
Other locations
- clathrin-coated pit 1 Publication
- clathrin-coated vesicle 1 Publication
Note: Colocalized with clathrin in the Golgi area.
Golgi apparatus
- Golgi apparatus Source: UniProtKB-SubCell
Plasma Membrane
- clathrin-coated pit Source: UniProtKB
- extrinsic component of presynaptic endocytic zone membrane Source: GO_Central
Other locations
- clathrin-coated vesicle Source: GO_Central
- synaptic vesicle Source: GO_Central
Keywords - Cellular componenti
Coated pit, Cytoplasmic vesicle, Golgi apparatus, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000187066 | 1 – 468 | Phosphatidylinositol-binding clathrin assembly protein LAPAdd BLAST | 468 |
Proteomic databases
PaxDbi | Q9VI75 |
PRIDEi | Q9VI75 |
Expressioni
Tissue specificityi
In embryos, expression is seen in central and peripheral nervous systems (brain and ventral nerve cord) and Garland cells. Coexpressed with clathrin at presynaptic boutons of neuromuscular junctions.1 Publication
Gene expression databases
Bgeei | FBgn0086372, Expressed in brain and 37 other tissues |
ExpressionAtlasi | Q9VI75, baseline and differential |
Genevisiblei | Q9VI75, DM |
Interactioni
Subunit structurei
Binds clathrin and phosphatidylinositol 4,5-bisphosphate.
By similarityGO - Molecular functioni
- clathrin binding Source: UniProtKB
- clathrin heavy chain binding Source: GO_Central
- SNARE binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 66058, 19 interactors |
ELMi | Q9VI75 |
IntActi | Q9VI75, 1 interactor |
STRINGi | 7227.FBpp0291394 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q9VI75 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9VI75 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 16 – 158 | ENTHPROSITE-ProRule annotationCuratedAdd BLAST | 143 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 438 – 468 | DisorderedSequence analysisAdd BLAST | 31 |
Sequence similaritiesi
Belongs to the PICALM/SNAP91 family.Curated
Phylogenomic databases
eggNOGi | KOG0251, Eukaryota |
GeneTreei | ENSGT00950000183068 |
HOGENOMi | CLU_014080_1_1_1 |
InParanoidi | Q9VI75 |
PhylomeDBi | Q9VI75 |
Family and domain databases
DisProti | DP02763 |
Gene3Di | 1.20.58.150, 1 hit 1.25.40.90, 1 hit |
InterProi | View protein in InterPro IPR011417, ANTH_dom IPR014712, ANTH_dom_sf IPR045192, AP180-like IPR013809, ENTH IPR008942, ENTH_VHS |
PANTHERi | PTHR22951, PTHR22951, 1 hit |
Pfami | View protein in Pfam PF07651, ANTH, 1 hit |
SMARTi | View protein in SMART SM00273, ENTH, 1 hit |
SUPFAMi | SSF48464, SSF48464, 1 hit |
PROSITEi | View protein in PROSITE PS50942, ENTH, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 8 potential isoforms that are computationally mapped.Show allAlign All
Q9VI75-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTMAGQTIND RLLAARHSLA GQGLAKSVCK ATTEECIGPK KKHLDYLVHC
60 70 80 90 100
TNEPNVSIPH LANLLIERSQ NANWVVVYKS LITTHHLMAY GNERFMQYLA
110 120 130 140 150
SSNSTFNLSS FLDKGTVQDG GMGVPGGRMG YDMSPFIRRY AKYLNEKSLS
160 170 180 190 200
YRAMAFDFCK VKRGKEEGSL RSMNAEKLLK TLPVLQAQLD ALLEFDCQSN
210 220 230 240 250
DLSNGVINMS FMLLFRDLIR LFACYNDGII NLLEKYFDMN KKHARDALDL
260 270 280 290 300
YKKFLVRMDR VGEFLKVAEN VGIDKGDIPD LTKAPSSLLD ALEQHLATLE
310 320 330 340 350
GRKVSAANTP TQSSSSAFGT AAASSKFDTT NGIDEQLKAQ VLAEEEAAMN
360 370 380 390 400
QYKSKVSSPT SSGAAGASAA LTNPFLSSPP AAQAGQPIVD LFGAASAQPA
410 420 430 440 450
AAAAATKASD DLLQLGNPFA DMFDASGGGA AAVGATGNAG DGTAKYDGGA
460
GSSPFDWGAT DDDGGAAQ
Computationally mapped potential isoform sequencesi
There are 8 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE1JJ78 | E1JJ78_DROME | Like-AP180, isoform D | lap AP180, BcDNA:GM10787, dAP-3, Dmel\CG2520, EP(3)3060 | 788 | Annotation score: | ||
B7Z0U7 | B7Z0U7_DROME | Like-AP180, isoform C | lap AP180, BcDNA:GM10787, dAP-3, Dmel\CG2520, EP(3)3060 | 758 | Annotation score: | ||
A0A0B4K631 | A0A0B4K631_DROME | Like-AP180, isoform F | lap AP180, BcDNA:GM10787, dAP-3, Dmel\CG2520, EP(3)3060 | 685 | Annotation score: | ||
A0A0B4K6V2 | A0A0B4K6V2_DROME | Like-AP180, isoform G | lap AP180, BcDNA:GM10787, dAP-3, Dmel\CG2520, EP(3)3060 | 673 | Annotation score: | ||
A0A0B4KF90 | A0A0B4KF90_DROME | Like-AP180, isoform J | lap AP180, BcDNA:GM10787, dAP-3, Dmel\CG2520, EP(3)3060 | 678 | Annotation score: | ||
A0A0B4KFE2 | A0A0B4KFE2_DROME | Like-AP180, isoform I | lap AP180, BcDNA:GM10787, dAP-3, Dmel\CG2520, EP(3)3060 | 776 | Annotation score: | ||
A0A0B4KGC6 | A0A0B4KGC6_DROME | Like-AP180, isoform H | lap AP180, BcDNA:GM10787, dAP-3, Dmel\CG2520, EP(3)3060 | 704 | Annotation score: | ||
A0A0B4K603 | A0A0B4K603_DROME | Like-AP180, isoform E | lap AP180, BcDNA:GM10787, dAP-3, Dmel\CG2520, EP(3)3060 | 572 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 51 | T → A in AAD08669 (PubMed:9883738).Curated | 1 | |
Sequence conflicti | 209 | M → I in AAM75091 (PubMed:12537569).Curated | 1 | |
Sequence conflicti | 372 | T → A in AAD08669 (PubMed:9883738).Curated | 1 | |
Sequence conflicti | 372 | T → A in AAM75091 (PubMed:12537569).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF075247 mRNA Translation: AAD08669.1 AE014297 Genomic DNA Translation: AAF54050.1 AY128498 mRNA Translation: AAM75091.1 |
RefSeqi | NP_524252.2, NM_079528.3 |
Genome annotation databases
EnsemblMetazoai | FBtr0081572; FBpp0081091; FBgn0086372 |
GeneIDi | 40863 |
KEGGi | dme:Dmel_CG2520 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF075247 mRNA Translation: AAD08669.1 AE014297 Genomic DNA Translation: AAF54050.1 AY128498 mRNA Translation: AAM75091.1 |
RefSeqi | NP_524252.2, NM_079528.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1HX8 | X-ray | 2.20 | A/B | 1-299 | [»] | |
SMRi | Q9VI75 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 66058, 19 interactors |
ELMi | Q9VI75 |
IntActi | Q9VI75, 1 interactor |
STRINGi | 7227.FBpp0291394 |
Proteomic databases
PaxDbi | Q9VI75 |
PRIDEi | Q9VI75 |
Genome annotation databases
EnsemblMetazoai | FBtr0081572; FBpp0081091; FBgn0086372 |
GeneIDi | 40863 |
KEGGi | dme:Dmel_CG2520 |
Organism-specific databases
CTDi | 7939 |
FlyBasei | FBgn0086372, lap |
VEuPathDBi | VectorBase:FBgn0086372 |
Phylogenomic databases
eggNOGi | KOG0251, Eukaryota |
GeneTreei | ENSGT00950000183068 |
HOGENOMi | CLU_014080_1_1_1 |
InParanoidi | Q9VI75 |
PhylomeDBi | Q9VI75 |
Enzyme and pathway databases
Reactomei | R-DME-432722, Golgi Associated Vesicle Biogenesis R-DME-8856825, Cargo recognition for clathrin-mediated endocytosis R-DME-8856828, Clathrin-mediated endocytosis |
Miscellaneous databases
BioGRID-ORCSi | 40863, 0 hits in 3 CRISPR screens |
EvolutionaryTracei | Q9VI75 |
GenomeRNAii | 40863 |
PROi | PR:Q9VI75 |
Gene expression databases
Bgeei | FBgn0086372, Expressed in brain and 37 other tissues |
ExpressionAtlasi | Q9VI75, baseline and differential |
Genevisiblei | Q9VI75, DM |
Family and domain databases
DisProti | DP02763 |
Gene3Di | 1.20.58.150, 1 hit 1.25.40.90, 1 hit |
InterProi | View protein in InterPro IPR011417, ANTH_dom IPR014712, ANTH_dom_sf IPR045192, AP180-like IPR013809, ENTH IPR008942, ENTH_VHS |
PANTHERi | PTHR22951, PTHR22951, 1 hit |
Pfami | View protein in Pfam PF07651, ANTH, 1 hit |
SMARTi | View protein in SMART SM00273, ENTH, 1 hit |
SUPFAMi | SSF48464, SSF48464, 1 hit |
PROSITEi | View protein in PROSITE PS50942, ENTH, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PICAL_DROME | |
Accessioni | Q9VI75Primary (citable) accession number: Q9VI75 Secondary accession number(s): O96528, Q8MQM1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 1, 2004 |
Last sequence update: | October 23, 2007 | |
Last modified: | February 23, 2022 | |
This is version 157 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families