UniProtKB - Q9VH48 (CARM1_DROME)
Protein
Probable histone-arginine methyltransferase CARMER
Gene
Art4
Organism
Drosophila melanogaster (Fruit fly)
Status
Functioni
Methylates (mono- and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in proteins. May methylate histone H3 at 'Arg-17' and activate transcription via chromatin remodeling. Coordinates ecdysone-mediated expression of cell death genes.2 Publications
Catalytic activityi
- L-arginyl-[protein] + 2 S-adenosyl-L-methionine = 2 H+ + Nω,Nω-dimethyl-L-arginyl-[protein] + 2 S-adenosyl-L-homocysteineBy similarityEC:2.1.1.319By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 154 | S-adenosyl-L-methionineBy similarity | 1 | |
Binding sitei | 163 | S-adenosyl-L-methionineBy similarity | 1 | |
Binding sitei | 187 | S-adenosyl-L-methionine; via carbonyl oxygenBy similarity | 1 | |
Binding sitei | 209 | S-adenosyl-L-methionineBy similarity | 1 | |
Binding sitei | 238 | S-adenosyl-L-methionineBy similarity | 1 | |
Binding sitei | 266 | S-adenosyl-L-methionineBy similarity | 1 |
GO - Molecular functioni
- histone-arginine N-methyltransferase activity Source: FlyBase
- histone methyltransferase activity Source: FlyBase
- protein-arginine omega-N asymmetric methyltransferase activity Source: FlyBase
- protein-arginine omega-N monomethyltransferase activity Source: FlyBase
GO - Biological processi
- histone arginine methylation Source: FlyBase
- histone methylation Source: FlyBase
- peptidyl-arginine methylation, to asymmetrical-dimethyl arginine Source: FlyBase
- positive regulation of ecdysone receptor-mediated signaling pathway Source: FlyBase
- positive regulation of transcription by RNA polymerase II Source: FlyBase
Keywordsi
Molecular function | Chromatin regulator, Methyltransferase, Transferase |
Biological process | Transcription, Transcription regulation |
Ligand | S-adenosyl-L-methionine |
Enzyme and pathway databases
Reactomei | R-DME-400206, Regulation of lipid metabolism by PPARalpha R-DME-9018519, Estrogen-dependent gene expression |
Names & Taxonomyi
Protein namesi | Recommended name: Probable histone-arginine methyltransferase CARMER (EC:2.1.1.319By similarity)Alternative name(s): Coactivator arginine methyltransferase for EcR/Usp Protein arginine N-methyltransferase 4 Short name: DART4 |
Gene namesi | Name:Art4 Synonyms:Carmer ORF Names:CG5358 |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Holometabola › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0037770, Art4 |
Subcellular locationi
Cytosol
- cytosol Source: FlyBase
Nucleus
- histone methyltransferase complex Source: FlyBase
- nucleus Source: UniProtKB
Other locations
- cytoplasm Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000249253 | 1 – 530 | Probable histone-arginine methyltransferase CARMERAdd BLAST | 530 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 501 | Asymmetric dimethylarginine; by autocatalysisBy similarity | 1 |
Post-translational modificationi
The dimethylated protein is the major form.By similarity
Keywords - PTMi
MethylationProteomic databases
PaxDbi | Q9VH48 |
PRIDEi | Q9VH48 |
Expressioni
Tissue specificityi
Present ubiquitously (at protein level). Expressed in the imaginal disks and in larval brains, and to a much lesser degree in the polytene larval tissue such as salivary glands.2 Publications
Developmental stagei
Expression is high in early embryos, reduced in late embryonic and larval stages, up-regulated at the early prepupal stage and then reduced until the adult stage where it is again up-regulated.1 Publication
Gene expression databases
Bgeei | FBgn0037770, Expressed in female gonad and 50 other tissues |
ExpressionAtlasi | Q9VH48, baseline and differential |
Genevisiblei | Q9VH48, DM |
Interactioni
Subunit structurei
Homodimer (By similarity).
Interacts with EcR.
By similarity1 PublicationProtein-protein interaction databases
BioGRIDi | 66376, 28 interactors |
IntActi | Q9VH48, 5 interactors |
STRINGi | 7227.FBpp0305840 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 141 – 450 | SAM-dependent MTase PRMT-typePROSITE-ProRule annotationAdd BLAST | 310 |
Sequence similaritiesi
Belongs to the class I-like SAM-binding methyltransferase superfamily. Protein arginine N-methyltransferase family.PROSITE-ProRule annotation
Phylogenomic databases
eggNOGi | KOG1500, Eukaryota |
GeneTreei | ENSGT00940000169584 |
HOGENOMi | CLU_017375_0_1_1 |
InParanoidi | Q9VH48 |
OMAi | FHILQTG |
OrthoDBi | 840669at2759 |
PhylomeDBi | Q9VH48 |
Family and domain databases
Gene3Di | 2.30.29.30, 1 hit |
InterProi | View protein in InterPro IPR025799, Arg_MeTrfase IPR011993, PH-like_dom_sf IPR029063, SAM-dependent_MTases |
SUPFAMi | SSF53335, SSF53335, 1 hit |
PROSITEi | View protein in PROSITE PS51678, SAM_MT_PRMT, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q9VH48-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSSLRPEEAR KLATAASVSP LSNCQFCGVV ISSIADEQKL EFTNKYKGSC
60 70 80 90 100
TLLCSYDSQG VVLRVVSDDD RSHVLKEYMI AADTDAAQMG RRSYAVSLDA
110 120 130 140 150
DNLVLRFASE QDQQLFRKVV ENVKHLRPKS VFSQRTEESS ASQYFQFYGY
160 170 180 190 200
LSQQQNMMQD YVRTSTYQRA ILGNAVDFQD KIVLDVGAGS GILSFFAVQA
210 220 230 240 250
GAAKVYAIEA SNMAQYAQQL VESNNVQHKI SVIPGKIEEI ELPEKVDVII
260 270 280 290 300
SEPMGYMLYN ERMLETYLHA RKWLKPQGKM YPTHGDLHIA PFSDESLYSE
310 320 330 340 350
QYNKANFWYQ SAFHGVDLTT LHKEGMKEYF RQPIVDTFDI RICMAKSVRH
360 370 380 390 400
VCDFLNDKED DLHLISIPLE FHILQTGICH GLAFWFDVEF SGSSQNVWLS
410 420 430 440 450
TSPTAPLTHW YQVRCLLPMP IFIKQGQTLT GRVLLEANRR QSYDVTIDLH
460 470 480 490 500
IEGTLISSSN TLDLKNPYFR YTGAPVQAPP GTSTQSPSEQ YWTQVDTQGS
510 520 530
RNSSSMLNGG ISVNGIGEGM DITHGLMHPH
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketF0JAI1 | F0JAI1_DROME | Type I protein arginine methyltrans... | Art4 CARM1, dArt 4, DART4, Dart4, Dmel\CG5358 | 530 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 121 | E → G in AAO45207 (PubMed:12537569).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE014297 Genomic DNA Translation: AAF54471.1 BT004851 mRNA Translation: AAO45207.1 |
RefSeqi | NP_001262445.1, NM_001275516.1 NP_649963.1, NM_141706.3 |
Genome annotation databases
EnsemblMetazoai | FBtr0082150; FBpp0081628; FBgn0037770 FBtr0333687; FBpp0305840; FBgn0037770 |
GeneIDi | 41219 |
KEGGi | dme:Dmel_CG5358 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE014297 Genomic DNA Translation: AAF54471.1 BT004851 mRNA Translation: AAO45207.1 |
RefSeqi | NP_001262445.1, NM_001275516.1 NP_649963.1, NM_141706.3 |
3D structure databases
SMRi | Q9VH48 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 66376, 28 interactors |
IntActi | Q9VH48, 5 interactors |
STRINGi | 7227.FBpp0305840 |
Proteomic databases
PaxDbi | Q9VH48 |
PRIDEi | Q9VH48 |
Genome annotation databases
EnsemblMetazoai | FBtr0082150; FBpp0081628; FBgn0037770 FBtr0333687; FBpp0305840; FBgn0037770 |
GeneIDi | 41219 |
KEGGi | dme:Dmel_CG5358 |
Organism-specific databases
CTDi | 420 |
FlyBasei | FBgn0037770, Art4 |
Phylogenomic databases
eggNOGi | KOG1500, Eukaryota |
GeneTreei | ENSGT00940000169584 |
HOGENOMi | CLU_017375_0_1_1 |
InParanoidi | Q9VH48 |
OMAi | FHILQTG |
OrthoDBi | 840669at2759 |
PhylomeDBi | Q9VH48 |
Enzyme and pathway databases
Reactomei | R-DME-400206, Regulation of lipid metabolism by PPARalpha R-DME-9018519, Estrogen-dependent gene expression |
Miscellaneous databases
BioGRID-ORCSi | 41219, 0 hits in 1 CRISPR screen |
GenomeRNAii | 41219 |
PROi | PR:Q9VH48 |
Gene expression databases
Bgeei | FBgn0037770, Expressed in female gonad and 50 other tissues |
ExpressionAtlasi | Q9VH48, baseline and differential |
Genevisiblei | Q9VH48, DM |
Family and domain databases
Gene3Di | 2.30.29.30, 1 hit |
InterProi | View protein in InterPro IPR025799, Arg_MeTrfase IPR011993, PH-like_dom_sf IPR029063, SAM-dependent_MTases |
SUPFAMi | SSF53335, SSF53335, 1 hit |
PROSITEi | View protein in PROSITE PS51678, SAM_MT_PRMT, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CARM1_DROME | |
Accessioni | Q9VH48Primary (citable) accession number: Q9VH48 Secondary accession number(s): Q86NL6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 5, 2006 |
Last sequence update: | May 1, 2000 | |
Last modified: | February 10, 2021 | |
This is version 162 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - SIMILARITY comments
Index of protein domains and families