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Protein

Glutathione S-transferase D4

Gene

GstD4

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles (PubMed:22082028). May be involved in detoxification (PubMed:22082028).1 Publication

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.1 Publication

Kineticsi

  1. KM=2.47 mM for glutathione1 Publication
  2. KM=2.04 mM for 1-chloro-2,4-dinitrobenzene1 Publication
  1. Vmax=0.26 µmol/min/mg enzyme with 1-chloro-2,4-dinitrobenzene as substrate1 Publication
  2. Vmax=1.45 µmol/min/mg enzyme with 4-hydroxy-2-nonenal as substrate1 Publication
  3. Vmax=99.5 nmol/min/mg enzyme with adrenochrome as substrate1 Publication
  4. Vmax=0.044 µmol/min/mg enzyme with phenethyl isothiocyanate as substrate1 Publication
  5. Vmax=0.047 µmol/min/mg enzyme with 2-hydroxyethyl disulfide as substrate1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei9GlutathioneBy similarity1

GO - Molecular functioni

  • glutathione transferase activity Source: FlyBase

GO - Biological processi

  • glutathione metabolic process Source: FlyBase
  • response to toxic substance Source: UniProtKB-KW

Keywordsi

Molecular functionTransferase
Biological processDetoxification

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione S-transferase D41 Publication (EC:2.5.1.181 Publication)
Gene namesi
Name:GstD4Imported
Synonyms:gstD23, GSTD4-4Imported
ORF Names:CG11512Imported
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0010040 GstD4

Subcellular locationi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001859561 – 215Glutathione S-transferase D4Add BLAST215

Proteomic databases

PaxDbiQ9VG96
PRIDEiQ9VG96

Expressioni

Gene expression databases

BgeeiFBgn0010040
GenevisibleiQ9VG96 DM

Interactioni

Subunit structurei

Homodimer.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
GstD6Q9VG943EBI-117276,EBI-152218

Protein-protein interaction databases

BioGridi71334, 8 interactors
DIPiDIP-19902N
IntActiQ9VG96, 6 interactors
STRINGi7227.FBpp0082043

Structurei

3D structure databases

ProteinModelPortaliQ9VG96
SMRiQ9VG96
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 80GST N-terminalAdd BLAST80
Domaini86 – 207GST C-terminalAdd BLAST122

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni50 – 52Glutathione bindingBy similarity3
Regioni64 – 66Glutathione bindingBy similarity3

Sequence similaritiesi

Belongs to the GST superfamily. Delta family.1 Publication

Phylogenomic databases

eggNOGiKOG0867 Eukaryota
COG0625 LUCA
GeneTreeiENSGT00540000069741
InParanoidiQ9VG96
KOiK00799
OMAiWKGLLQM
OrthoDBiEOG091G0MS3
PhylomeDBiQ9VG96

Family and domain databases

InterProiView protein in InterPro
IPR010987 Glutathione-S-Trfase_C-like
IPR036282 Glutathione-S-Trfase_C_sf
IPR004045 Glutathione_S-Trfase_N
IPR004046 GST_C
IPR036249 Thioredoxin-like_sf
PfamiView protein in Pfam
PF00043 GST_C, 1 hit
PF02798 GST_N, 1 hit
SUPFAMiSSF47616 SSF47616, 1 hit
SSF52833 SSF52833, 1 hit
PROSITEiView protein in PROSITE
PS50405 GST_CTER, 1 hit
PS50404 GST_NTER, 1 hit

Sequencei

Sequence statusi: Complete.

Q9VG96-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDFYYSPRSS GSRTIIMVAK ALGLELNKKQ LRITEGEHLK PEFLKLNPQH
60 70 80 90 100
TIPTLVDNGF AIWESRAIAV YLVEKYGKDD SLFPNDPQKR ALINQRLYFD
110 120 130 140 150
MGTLHDSFMK YYYPFIRTGQ LGNAENYKKV EAAFEFLDIF LEGQDYVAGS
160 170 180 190 200
QLTVADIAIL SSVSTFEVVE FDISKYPNVA RWYANAKKIT PGWDENWKGL
210
LQMKTMYEAQ KASLK
Length:215
Mass (Da):24,736
Last modified:May 1, 2000 - v1
Checksum:i9556E0A1A54BBE1F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti142E → V in M97702 (PubMed:7683659).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M97702 Genomic DNA No translation available.
AE014297 Genomic DNA Translation: AAF54789.1
AY071648 mRNA Translation: AAL49270.1
PIRiE46681
RefSeqiNP_524913.1, NM_080174.4
UniGeneiDm.1754

Genome annotation databases

EnsemblMetazoaiFBtr0082571; FBpp0082043; FBgn0010040
GeneIDi48337
KEGGidme:Dmel_CG11512

Similar proteinsi

Entry informationi

Entry nameiGSTD4_DROME
AccessioniPrimary (citable) accession number: Q9VG96
Secondary accession number(s): Q541F8, Q9TX90
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 2000
Last modified: July 18, 2018
This is version 137 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

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