UniProtKB - Q9VF30 (MCMR_DROME)
Protein
DNA replication licensing factor REC
Gene
rec
Organism
Drosophila melanogaster (Fruit fly)
Status
Functioni
Required for meiotic DNA recombination in females. Probably not involved in DNA repair and recombination in somatic cells.1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 281 – 308 | C4-typeSequence analysisAdd BLAST | 28 | |
Nucleotide bindingi | 473 – 480 | ATPSequence analysis | 8 |
GO - Molecular functioni
- ATPase activity Source: UniProtKB
- ATP binding Source: UniProtKB-KW
- DNA-dependent ATPase activity Source: UniProtKB
- DNA helicase activity Source: UniProtKB
- DNA replication origin binding Source: GO_Central
- metal ion binding Source: UniProtKB-KW
- single-stranded DNA binding Source: GO_Central
GO - Biological processi
- cell division Source: UniProtKB-KW
- DNA replication initiation Source: UniProtKB
- reciprocal meiotic recombination Source: UniProtKB
Keywordsi
Molecular function | DNA-binding |
Biological process | Cell cycle, Cell division, DNA recombination, Meiosis |
Ligand | ATP-binding, Metal-binding, Nucleotide-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-DME-176187, Activation of ATR in response to replication stress R-DME-68689, CDC6 association with the ORC:origin complex R-DME-68827, CDT1 association with the CDC6:ORC:origin complex R-DME-68867, Assembly of the pre-replicative complex R-DME-68949, Orc1 removal from chromatin R-DME-68962, Activation of the pre-replicative complex |
Names & Taxonomyi
Protein namesi | Recommended name: DNA replication licensing factor RECAlternative name(s): Protein mini chromosome maintenance-related Protein recombination-defective |
Gene namesi | Name:rec ORF Names:CG31293 |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Holometabola › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0003227, rec |
Subcellular locationi
Nucleus
- Nucleus By similarity
Nucleus
- nucleus Source: GO_Central
Other locations
- MCM complex Source: GO_Central
Keywords - Cellular componenti
NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 455 | S → F in rec3; temperature-sensitive allele. Recombination-defective. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000194127 | 1 – 885 | DNA replication licensing factor RECAdd BLAST | 885 |
Proteomic databases
PaxDbi | Q9VF30 |
Expressioni
Gene expression databases
Bgeei | FBgn0003227, Expressed in embryo and 15 other tissues |
ExpressionAtlasi | Q9VF30, baseline and differential |
Genevisiblei | Q9VF30, DM |
Interactioni
Binary interactionsi
Q9VF30
With | #Exp. | IntAct |
---|---|---|
mei-217 [Q86B50] | 2 | EBI-99278,EBI-15133128 |
Protein-protein interaction databases
BioGRIDi | 72108, 4 interactors |
DIPi | DIP-61892N |
IntActi | Q9VF30, 4 interactors |
STRINGi | 7227.FBpp0082647 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 430 – 627 | MCMAdd BLAST | 198 |
Sequence similaritiesi
Belongs to the MCM family.Curated
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 281 – 308 | C4-typeSequence analysisAdd BLAST | 28 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG0478, Eukaryota |
GeneTreei | ENSGT01010000222249 |
HOGENOMi | CLU_000995_7_2_1 |
InParanoidi | Q9VF30 |
OMAi | FYFRRNG |
OrthoDBi | 266497at2759 |
PhylomeDBi | Q9VF30 |
Family and domain databases
InterProi | View protein in InterPro IPR031327, MCM IPR001208, MCM_dom IPR041562, MCM_lid IPR033762, MCM_OB IPR012340, NA-bd_OB-fold IPR027417, P-loop_NTPase |
PANTHERi | PTHR11630, PTHR11630, 1 hit |
Pfami | View protein in Pfam PF00493, MCM, 1 hit PF17855, MCM_lid, 1 hit PF17207, MCM_OB, 1 hit |
PRINTSi | PR01657, MCMFAMILY |
SMARTi | View protein in SMART SM00350, MCM, 1 hit |
SUPFAMi | SSF50249, SSF50249, 1 hit SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS50051, MCM_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q9VF30-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNPTLSRGGA PSRFVRGRGR GGGAYRPYFY FRRNGRVIPA GGNRQPNQGE
60 70 80 90 100
PGAPDAPSVP PATRQPRGWS RTAGKRGRNA HLDCSFLRPE NYAAPEDGLQ
110 120 130 140 150
VQSIAVDNPR AYSGWRLYFL REKYEEGNEL ATRIMAVEAY YQRNPHTFDF
160 170 180 190 200
VMIRDRGFFP LCAAGIKSDA QLKEVWPSLS EDIREHPLRT LGTLSLAMHT
210 220 230 240 250
VVVNHQLDCN DSTTAMVSTP EQYVLPTPRV RKIYARPDDF VPVESIEGIS
260 270 280 290 300
HSRVDTLFSI RGFVSNVGEP SYSLTWQAFR CSRCQMEIAM RQRGTFQPRP
310 320 330 340 350
YQCKRSECVA RDEFVPLRSS PYTRLSIRQI IRVEESSLNL VHDFETSMPA
360 370 380 390 400
EMDVELRHDL VDAVRVGQEV VVTGILKLQE LGDDTTTGDT SNQMQPYLKA
410 420 430 440 450
VSIRDASSIK REFSERDLEA IVMINAEPNS FKLLVQSIAP EVYGHELPKA
460 470 480 490 500
ACLLSLLGGK GAETEAINVL LVGDPGIGKT KILQSCAQIA ERGAHVSGKR
510 520 530 540 550
GAQSAQQLGV TFAGRNKRVL QAGSLMMASG GGHCTLDDVD KLASKQAVLL
560 570 580 590 600
QCLQSEEVNL PLAGAFASFP AQPSVIACAN PQRGQYDEGR YLLQNINISP
610 620 630 640 650
SLLREFHLVY ILLDKPSERD MSLTAHVRAL HAGARKRARI AARYALKPKM
660 670 680 690 700
SDSMCEVSLN VPAAGKDDDT IKTEDDNDSI MQQDYDLDKR LEVLPEEGDL
710 720 730 740 750
DLLPPILIKK FLSYARQELN PVLNEDASNA VLRYFLELKG SCNLDEDVSS
760 770 780 790 800
QIGAGQLLAI IHLSQARARL DLSHVVSPQH VRDVIALLTE SITQTSLKEG
810 820 830 840 850
SSRQGTRGGG GAGGGAGKSA QLRNFLELTK RRSAALGRRI FEFDELKEIG
860 870 880
TRAGILTGFS QLVEMANLGG YLLMKGANMY EVVPD
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A0B4KH79 | A0A0B4KH79_DROME | Recombination-defective, isoform B | rec CG14875, CG4828, Dmel\CG31293, MCM8, rec-1 | 885 | Annotation score: |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 118 | Y → H1 Publication | 1 | |
Natural varianti | 422 | V → A1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB120941 mRNA Translation: BAC92698.1 AB120942 mRNA Translation: BAC92699.1 AB120943 mRNA Translation: BAC92700.1 AB120944 Genomic DNA Translation: BAC92701.1 AB120945 Genomic DNA Translation: BAC92702.1 AB120946 Genomic DNA Translation: BAC92703.1 AB120947 Genomic DNA Translation: BAC92704.1 AB120948 Genomic DNA Translation: BAC92705.1 AB120949 Genomic DNA Translation: BAC92706.1 AB120950 Genomic DNA Translation: BAC92707.1 AB120951 mRNA Translation: BAC92708.1 AB120952 Genomic DNA Translation: BAC92709.1 AB120953 mRNA Translation: BAC92710.1 AE014297 Genomic DNA Translation: AAF55231.2 |
RefSeqi | NP_001262615.1, NM_001275686.1 NP_732072.1, NM_169674.2 |
Genome annotation databases
EnsemblMetazoai | FBtr0083193; FBpp0082647; FBgn0003227 FBtr0334595; FBpp0306662; FBgn0003227 |
GeneIDi | 49241 |
KEGGi | dme:Dmel_CG31293 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB120941 mRNA Translation: BAC92698.1 AB120942 mRNA Translation: BAC92699.1 AB120943 mRNA Translation: BAC92700.1 AB120944 Genomic DNA Translation: BAC92701.1 AB120945 Genomic DNA Translation: BAC92702.1 AB120946 Genomic DNA Translation: BAC92703.1 AB120947 Genomic DNA Translation: BAC92704.1 AB120948 Genomic DNA Translation: BAC92705.1 AB120949 Genomic DNA Translation: BAC92706.1 AB120950 Genomic DNA Translation: BAC92707.1 AB120951 mRNA Translation: BAC92708.1 AB120952 Genomic DNA Translation: BAC92709.1 AB120953 mRNA Translation: BAC92710.1 AE014297 Genomic DNA Translation: AAF55231.2 |
RefSeqi | NP_001262615.1, NM_001275686.1 NP_732072.1, NM_169674.2 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 72108, 4 interactors |
DIPi | DIP-61892N |
IntActi | Q9VF30, 4 interactors |
STRINGi | 7227.FBpp0082647 |
Proteomic databases
PaxDbi | Q9VF30 |
Genome annotation databases
EnsemblMetazoai | FBtr0083193; FBpp0082647; FBgn0003227 FBtr0334595; FBpp0306662; FBgn0003227 |
GeneIDi | 49241 |
KEGGi | dme:Dmel_CG31293 |
Organism-specific databases
CTDi | 49241 |
FlyBasei | FBgn0003227, rec |
Phylogenomic databases
eggNOGi | KOG0478, Eukaryota |
GeneTreei | ENSGT01010000222249 |
HOGENOMi | CLU_000995_7_2_1 |
InParanoidi | Q9VF30 |
OMAi | FYFRRNG |
OrthoDBi | 266497at2759 |
PhylomeDBi | Q9VF30 |
Enzyme and pathway databases
Reactomei | R-DME-176187, Activation of ATR in response to replication stress R-DME-68689, CDC6 association with the ORC:origin complex R-DME-68827, CDT1 association with the CDC6:ORC:origin complex R-DME-68867, Assembly of the pre-replicative complex R-DME-68949, Orc1 removal from chromatin R-DME-68962, Activation of the pre-replicative complex |
Miscellaneous databases
BioGRID-ORCSi | 49241, 0 hits in 1 CRISPR screen |
GenomeRNAii | 49241 |
PROi | PR:Q9VF30 |
Gene expression databases
Bgeei | FBgn0003227, Expressed in embryo and 15 other tissues |
ExpressionAtlasi | Q9VF30, baseline and differential |
Genevisiblei | Q9VF30, DM |
Family and domain databases
InterProi | View protein in InterPro IPR031327, MCM IPR001208, MCM_dom IPR041562, MCM_lid IPR033762, MCM_OB IPR012340, NA-bd_OB-fold IPR027417, P-loop_NTPase |
PANTHERi | PTHR11630, PTHR11630, 1 hit |
Pfami | View protein in Pfam PF00493, MCM, 1 hit PF17855, MCM_lid, 1 hit PF17207, MCM_OB, 1 hit |
PRINTSi | PR01657, MCMFAMILY |
SMARTi | View protein in SMART SM00350, MCM, 1 hit |
SUPFAMi | SSF50249, SSF50249, 1 hit SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS50051, MCM_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MCMR_DROME | |
Accessioni | Q9VF30Primary (citable) accession number: Q9VF30 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 10, 2004 |
Last sequence update: | October 1, 2002 | |
Last modified: | February 10, 2021 | |
This is version 149 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - SIMILARITY comments
Index of protein domains and families