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UniProtKB - Q9VEI3 (S35B2_DROME)
Protein
Adenosine 3'-phospho 5'-phosphosulfate transporter 1
Gene
sll
Organism
Drosophila melanogaster (Fruit fly)
Status
Functioni
Mediates the transport of adenosine 3'-phospho 5'-phosphosulfate (PAPS), from cytosol into Golgi. PAPS is a universal sulfuryl donor for sulfation events that take place in the Golgi. Required for the dorsoventral patterning, suggesting that it mediates the transport of the sulfate donor required for the sulfotransferase activity of pip (pipe).
2 PublicationsKineticsi
- KM=1.2 µM for PAPS1 Publication
GO - Molecular functioni
- 3'-phosphoadenosine 5'-phosphosulfate transmembrane transporter activity Source: UniProtKB
- transmembrane transporter activity Source: GO_Central
GO - Biological processi
- 3'-phosphoadenosine 5'-phosphosulfate transport Source: UniProtKB
- heparan sulfate proteoglycan biosynthetic process Source: FlyBase
- oocyte dorsal/ventral axis specification Source: FlyBase
Keywordsi
Molecular function | Developmental protein |
Biological process | Transport |
Enzyme and pathway databases
Reactomei | R-DME-174362, Transport and synthesis of PAPS R-DME-727802, Transport of nucleotide sugars |
SABIO-RKi | Q9VEI3 |
Protein family/group databases
TCDBi | 2.A.7.11.2, the drug/metabolite transporter (dmt) superfamily |
Names & Taxonomyi
Protein namesi | Recommended name: Adenosine 3'-phospho 5'-phosphosulfate transporter 1Alternative name(s): PAPS transporter 1 Protein slalom |
Gene namesi | Name:sll Synonyms:PAPST1 ORF Names:CG7623 |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0038524, sll |
VEuPathDBi | VectorBase:FBgn0038524 |
Subcellular locationi
Golgi apparatus
- Golgi apparatus membrane By similarity; Multi-pass membrane protein By similarity
Endoplasmic reticulum
- integral component of endoplasmic reticulum membrane Source: GO_Central
Golgi apparatus
- Golgi apparatus Source: UniProtKB
- integral component of Golgi membrane Source: GO_Central
Other locations
- integral component of membrane Source: UniProtKB
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 13 – 33 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 61 – 81 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 142 – 162 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 185 – 205 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 270 – 290 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 299 – 319 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 339 – 359 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 370 – 390 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 391 – 407 | HelicalSequence analysisAdd BLAST | 17 | |
Transmembranei | 424 – 444 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Golgi apparatus, MembranePathology & Biotechi
Disruption phenotypei
Flies display defects in wingless (wg) and hedgehog (hh) signaling, probably due to the lack of sulfation of glycosaminoglycans by sulfotransferases such as sfl.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000213378 | 1 – 465 | Adenosine 3'-phospho 5'-phosphosulfate transporter 1Add BLAST | 465 |
Proteomic databases
PaxDbi | Q9VEI3 |
Expressioni
Tissue specificityi
Expressed throughout embryogenesis. During oogenesis, it is expressed strongly in the nurse cells of the germline. Maternally expressed at the syncytial blastoderm stage. Zygotically expressed, from after germ-band elongation in the invaginating salivary gland placodes. Remains expressed predominantly in this tissue throughout embryogenesis, but low-level expression may also be present throughout the embryo.1 Publication
Developmental stagei
Expressed both maternally and zygotically.1 Publication
Gene expression databases
Bgeei | FBgn0038524, Expressed in embryo and 38 other tissues |
ExpressionAtlasi | Q9VEI3, baseline and differential |
Genevisiblei | Q9VEI3, DM |
Interactioni
Protein-protein interaction databases
BioGRIDi | 67147, 4 interactors |
IntActi | Q9VEI3, 7 interactors |
STRINGi | 7227.FBpp0082903 |
Structurei
3D structure databases
AlphaFoldDBi | Q9VEI3 |
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1581, Eukaryota |
GeneTreei | ENSGT00940000157927 |
HOGENOMi | CLU_036019_3_1_1 |
InParanoidi | Q9VEI3 |
OMAi | CGAIGQV |
OrthoDBi | 820879at2759 |
PhylomeDBi | Q9VEI3 |
Family and domain databases
InterProi | View protein in InterPro IPR013657, UAA |
PANTHERi | PTHR10778, PTHR10778, 1 hit |
Pfami | View protein in Pfam PF08449, UAA, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q9VEI3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MYAYNKMGRV PELVICSFIV VTLLVIHFFS DLLRASLGGY YNQDVTLSQL
60 70 80 90 100
VESQNSDYAW FLKLLVNCFG YSCVFVPGFL IYKYVGRINY LERGNKTFLH
110 120 130 140 150
KAINMCITGN SGYDQLDAGT STADKDRPAA STAPKRTSSQ EAVQLLWCFG
160 170 180 190 200
GLMISYLTWG VLQEKIMTQN YLNFTGESAK FKDSQFLVFS NRLLAFLVAL
210 220 230 240 250
AYLQWQPSPV RHRAPLYKYS YASFSNIMSA WFQYEALKFV NFPTQVLAKS
260 270 280 290 300
CKIIPVMLMG KIMSKAKYES YEYVTALLIS LGMIFFMSGS SDSSKASGVT
310 320 330 340 350
TLTGIFLLSM YMVFDSFTAN WQGSLFKSYG MTPLQMMCGV NLFSSIFTGA
360 370 380 390 400
SLSMQGGFMD SLAFATEHPK FVFDMVVLSV CSAVGQLFIY HTIDVFGPVV
410 420 430 440 450
FTIIMTLRQA VAIMLSCFIY QHSISLLGIF GVLIVFVAIF LRVYCTQRLR
460
AIRKRAEANK PKMAV
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketH0RNN2 | H0RNN2_DROME | FI17505p1 | sll 14, 1422, anon-WO0118547.555, anon-WO0172774.85, Dmel\CG7623 | 465 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 22 | T → S in AAK93522 (PubMed:12537569).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB107958 mRNA Translation: BAC79118.1 AE014297 Genomic DNA Translation: AAF55438.1 AY052098 mRNA Translation: AAK93522.1 |
RefSeqi | NP_001247153.1, NM_001260224.2 NP_524389.1, NM_079665.4 |
Genome annotation databases
EnsemblMetazoai | FBtr0083462; FBpp0082903; FBgn0038524 FBtr0305308; FBpp0293851; FBgn0038524 |
GeneIDi | 42115 |
KEGGi | dme:Dmel_CG7623 |
UCSCi | CG7623-RA, d. melanogaster |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB107958 mRNA Translation: BAC79118.1 AE014297 Genomic DNA Translation: AAF55438.1 AY052098 mRNA Translation: AAK93522.1 |
RefSeqi | NP_001247153.1, NM_001260224.2 NP_524389.1, NM_079665.4 |
3D structure databases
AlphaFoldDBi | Q9VEI3 |
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 67147, 4 interactors |
IntActi | Q9VEI3, 7 interactors |
STRINGi | 7227.FBpp0082903 |
Protein family/group databases
TCDBi | 2.A.7.11.2, the drug/metabolite transporter (dmt) superfamily |
Proteomic databases
PaxDbi | Q9VEI3 |
Protocols and materials databases
DNASUi | 42115 |
Genome annotation databases
EnsemblMetazoai | FBtr0083462; FBpp0082903; FBgn0038524 FBtr0305308; FBpp0293851; FBgn0038524 |
GeneIDi | 42115 |
KEGGi | dme:Dmel_CG7623 |
UCSCi | CG7623-RA, d. melanogaster |
Organism-specific databases
CTDi | 42115 |
FlyBasei | FBgn0038524, sll |
VEuPathDBi | VectorBase:FBgn0038524 |
Phylogenomic databases
eggNOGi | KOG1581, Eukaryota |
GeneTreei | ENSGT00940000157927 |
HOGENOMi | CLU_036019_3_1_1 |
InParanoidi | Q9VEI3 |
OMAi | CGAIGQV |
OrthoDBi | 820879at2759 |
PhylomeDBi | Q9VEI3 |
Enzyme and pathway databases
Reactomei | R-DME-174362, Transport and synthesis of PAPS R-DME-727802, Transport of nucleotide sugars |
SABIO-RKi | Q9VEI3 |
Miscellaneous databases
BioGRID-ORCSi | 42115, 0 hits in 3 CRISPR screens |
GenomeRNAii | 42115 |
PROi | PR:Q9VEI3 |
Gene expression databases
Bgeei | FBgn0038524, Expressed in embryo and 38 other tissues |
ExpressionAtlasi | Q9VEI3, baseline and differential |
Genevisiblei | Q9VEI3, DM |
Family and domain databases
InterProi | View protein in InterPro IPR013657, UAA |
PANTHERi | PTHR10778, PTHR10778, 1 hit |
Pfami | View protein in Pfam PF08449, UAA, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | S35B2_DROME | |
Accessioni | Q9VEI3Primary (citable) accession number: Q9VEI3 Secondary accession number(s): Q960E7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 7, 2005 |
Last sequence update: | May 1, 2000 | |
Last modified: | May 25, 2022 | |
This is version 126 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - SIMILARITY comments
Index of protein domains and families