UniProtKB - Q9VE17 (RU1C_DROME)
Protein
U1 small nuclear ribonucleoprotein C
Gene
snRNP-U1-C
Organism
Drosophila melanogaster (Fruit fly)
Status
Functioni
Component of the spliceosomal U1 snRNP, which is essential for recognition of the pre-mRNA 5' splice-site and the subsequent assembly of the spliceosome. U1-C is directly involved in initial 5' splice-site recognition for both constitutive and regulated alternative splicing. The interaction with the 5' splice-site seems to precede base-pairing between the pre-mRNA and the U1 snRNA. Stimulates commitment or early (E) complex formation by stabilizing the base pairing of the 5' end of the U1 snRNA and the 5' splice-site region (By similarity). Regulates alternative splicing of a distinct group of target genes.UniRule annotation2 Publications
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 4 – 36 | Matrin-typeUniRule annotationAdd BLAST | 33 |
GO - Molecular functioni
- mRNA binding Source: UniProtKB-UniRule
- pre-mRNA 5'-splice site binding Source: GO_Central
- U1 snRNA binding Source: UniProtKB-UniRule
- zinc ion binding Source: UniProtKB-UniRule
GO - Biological processi
- mRNA 5'-splice site recognition Source: GO_Central
- mRNA splicing, via spliceosome Source: FlyBase
- regulation of alternative mRNA splicing, via spliceosome Source: FlyBase
- spliceosomal snRNP assembly Source: UniProtKB-UniRule
Keywordsi
Molecular function | Ribonucleoprotein, RNA-binding |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-DME-72163, mRNA Splicing - Major Pathway |
Names & Taxonomyi
Protein namesi | Recommended name: U1 small nuclear ribonucleoprotein CUniRule annotationShort name: U1 snRNP CUniRule annotation Short name: U1-CUniRule annotation Short name: U1CUniRule annotation |
Gene namesi | Name:snRNP-U1-CUniRule annotation ORF Names:CG5454 |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Holometabola › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0261792, snRNP-U1-C |
Subcellular locationi
Nucleus
- Nucleus UniRule annotation
Nucleus
- commitment complex Source: UniProtKB-UniRule
- nucleus Source: FlyBase
- small nuclear ribonucleoprotein complex Source: FlyBase
- U1 snRNP Source: FlyBase
- U2-type prespliceosome Source: UniProtKB-UniRule
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000414261 | 1 – 145 | U1 small nuclear ribonucleoprotein CAdd BLAST | 145 |
Proteomic databases
PaxDbi | Q9VE17 |
PRIDEi | Q9VE17 |
Expressioni
Gene expression databases
Bgeei | FBgn0261792, Expressed in embryo and 46 other tissues |
ExpressionAtlasi | Q9VE17, baseline and differential |
Genevisiblei | Q9VE17, DM |
Interactioni
Subunit structurei
U1 snRNP is composed of the 7 core Sm proteins B/B', D1, D2, D3, E, F and G that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP, and at least 3 U1 snRNP-specific proteins U1-70K, U1-A and U1-C. U1-C interacts with U1 snRNA and the 5' splice-site region of the pre-mRNA.
UniRule annotationProtein-protein interaction databases
BioGRIDi | 67277, 8 interactors |
IntActi | Q9VE17, 9 interactors |
STRINGi | 7227.FBpp0083134 |
Family & Domainsi
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 66 – 138 | Met/Pro-richAdd BLAST | 73 |
Sequence similaritiesi
Belongs to the U1 small nuclear ribonucleoprotein C family.UniRule annotation
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 4 – 36 | Matrin-typeUniRule annotationAdd BLAST | 33 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG3454, Eukaryota |
GeneTreei | ENSGT01000000214957 |
HOGENOMi | CLU_079697_3_1_1 |
InParanoidi | Q9VE17 |
OMAi | VVEYYQQ |
OrthoDBi | 1551234at2759 |
PhylomeDBi | Q9VE17 |
Family and domain databases
HAMAPi | MF_03153, U1_C, 1 hit |
InterProi | View protein in InterPro IPR000690, Matrin/U1-C_Znf_C2H2 IPR003604, Matrin/U1-like-C_Znf_C2H2 IPR013085, U1-CZ_Znf_C2H2 IPR017340, U1_snRNP-C IPR036236, Znf_C2H2_sf |
PANTHERi | PTHR31148, PTHR31148, 1 hit |
Pfami | View protein in Pfam PF06220, zf-U1, 1 hit |
PIRSFi | PIRSF037969, U1_snRNP-C, 1 hit |
SMARTi | View protein in SMART SM00451, ZnF_U1, 1 hit |
SUPFAMi | SSF57667, SSF57667, 1 hit |
PROSITEi | View protein in PROSITE PS50171, ZF_MATRIN, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9VE17-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPKYYCDYCD TYLTHDSPSV RKTHCTGRKH RDNVKFYYQK WMEEQAQHLI
60 70 80 90 100
DATTAAFKAG KITNNPFAGG PGGAPPKPAG VSIPPPNMGA PPRPGMPGMP
110 120 130 140
YMPPLMNPMM GMRPPPIMNP MAMMGPPPPL GTIPGVRPGI MNGPK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE014297 Genomic DNA Translation: AAF55616.1 BT003460 mRNA Translation: AAO39463.1 |
RefSeqi | NP_650767.1, NM_142510.3 |
Genome annotation databases
EnsemblMetazoai | FBtr0083720; FBpp0083134; FBgn0261792 |
GeneIDi | 42274 |
KEGGi | dme:Dmel_CG5454 |
UCSCi | CG5454-RA, d. melanogaster |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE014297 Genomic DNA Translation: AAF55616.1 BT003460 mRNA Translation: AAO39463.1 |
RefSeqi | NP_650767.1, NM_142510.3 |
3D structure databases
SMRi | Q9VE17 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 67277, 8 interactors |
IntActi | Q9VE17, 9 interactors |
STRINGi | 7227.FBpp0083134 |
Proteomic databases
PaxDbi | Q9VE17 |
PRIDEi | Q9VE17 |
Genome annotation databases
EnsemblMetazoai | FBtr0083720; FBpp0083134; FBgn0261792 |
GeneIDi | 42274 |
KEGGi | dme:Dmel_CG5454 |
UCSCi | CG5454-RA, d. melanogaster |
Organism-specific databases
CTDi | 42274 |
FlyBasei | FBgn0261792, snRNP-U1-C |
Phylogenomic databases
eggNOGi | KOG3454, Eukaryota |
GeneTreei | ENSGT01000000214957 |
HOGENOMi | CLU_079697_3_1_1 |
InParanoidi | Q9VE17 |
OMAi | VVEYYQQ |
OrthoDBi | 1551234at2759 |
PhylomeDBi | Q9VE17 |
Enzyme and pathway databases
Reactomei | R-DME-72163, mRNA Splicing - Major Pathway |
Miscellaneous databases
BioGRID-ORCSi | 42274, 0 hits in 1 CRISPR screen |
GenomeRNAii | 42274 |
PROi | PR:Q9VE17 |
Gene expression databases
Bgeei | FBgn0261792, Expressed in embryo and 46 other tissues |
ExpressionAtlasi | Q9VE17, baseline and differential |
Genevisiblei | Q9VE17, DM |
Family and domain databases
HAMAPi | MF_03153, U1_C, 1 hit |
InterProi | View protein in InterPro IPR000690, Matrin/U1-C_Znf_C2H2 IPR003604, Matrin/U1-like-C_Znf_C2H2 IPR013085, U1-CZ_Znf_C2H2 IPR017340, U1_snRNP-C IPR036236, Znf_C2H2_sf |
PANTHERi | PTHR31148, PTHR31148, 1 hit |
Pfami | View protein in Pfam PF06220, zf-U1, 1 hit |
PIRSFi | PIRSF037969, U1_snRNP-C, 1 hit |
SMARTi | View protein in SMART SM00451, ZnF_U1, 1 hit |
SUPFAMi | SSF57667, SSF57667, 1 hit |
PROSITEi | View protein in PROSITE PS50171, ZF_MATRIN, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RU1C_DROME | |
Accessioni | Q9VE17Primary (citable) accession number: Q9VE17 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 14, 2011 |
Last sequence update: | May 1, 2000 | |
Last modified: | December 2, 2020 | |
This is version 146 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - SIMILARITY comments
Index of protein domains and families