UniProtKB - Q9VDQ7 (NAA25_DROME)
Protein
Phagocyte signaling-impaired protein
Gene
psidin
Organism
Drosophila melanogaster (Fruit fly)
Status
Functioni
Non-catalytic subunit of the NatB complex which catalyzes acetylation of the N-terminal methionine residues of proteins beginning with Met-Asp or Met-Glu (By similarity). Has 2 roles in the larval immune response: required both for the phagocytic degradation of internalized bacteria and for the induction of Defensin in the fat body. Within the phagocytic blood cells, has a role in detection of infection and activation of the humoral immune response.By similarity1 Publication
GO - Molecular functioni
- actin filament binding Source: FlyBase
GO - Biological processi
- actin filament depolymerization Source: FlyBase
- activation of immune response Source: FlyBase
- border follicle cell migration Source: FlyBase
- immune response Source: UniProtKB
- innate immune response Source: UniProtKB-KW
- negative regulation of neuron apoptotic process Source: FlyBase
- N-terminal peptidyl-methionine acetylation Source: GO_Central
- phagocytosis, engulfment Source: UniProtKB
- positive regulation of lamellipodium assembly Source: FlyBase
- sensory neuron axon guidance Source: FlyBase
Keywordsi
Molecular function | Antimicrobial |
Biological process | Immunity, Innate immunity |
Names & Taxonomyi
Protein namesi | Recommended name: Phagocyte signaling-impaired proteinAlternative name(s): N-terminal acetyltransferase B complex subunit MDM20 homolog N-terminal acetyltransferase B complex subunit NAA25 homolog |
Gene namesi | Name:psidin1 Publication ORF Names:CG4845 |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Holometabola › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0243511, psidin |
Subcellular locationi
Lysosome
- Lysosome 1 Publication
Note: Blood cell lysosomes.1 Publication
Lysosome
- lysosome Source: UniProtKB
Other locations
- cytoplasm Source: FlyBase
- growth cone lamellipodium Source: FlyBase
- NatB complex Source: FlyBase
- neuron projection Source: FlyBase
Keywords - Cellular componenti
LysosomePathology & Biotechi
Disruption phenotypei
Flies are deficient in the induction of Defensin whether the infection was with a Gram-negative or Gram-positive micro-organism.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000314625 | 1 – 948 | Phagocyte signaling-impaired proteinAdd BLAST | 948 |
Proteomic databases
PaxDbi | Q9VDQ7 |
PRIDEi | Q9VDQ7 |
Expressioni
Tissue specificityi
Expressed in the fat body after immune challenge.1 Publication
Gene expression databases
Bgeei | FBgn0243511, Expressed in embryonic/larval hemocyte (Drosophila) and 38 other tissues |
ExpressionAtlasi | Q9VDQ7, baseline and differential |
Genevisiblei | Q9VDQ7, DM |
Interactioni
Subunit structurei
Component of the N-terminal acetyltransferase B (NatB) complex.
By similarityGO - Molecular functioni
- actin filament binding Source: FlyBase
Protein-protein interaction databases
BioGRIDi | 67375, 14 interactors |
IntActi | Q9VDQ7, 4 interactors |
STRINGi | 7227.FBpp0083238 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 46 – 79 | TPR 1Sequence analysisAdd BLAST | 34 | |
Repeati | 80 – 113 | TPR 2Sequence analysisAdd BLAST | 34 | |
Repeati | 523 – 560 | TPR 3Sequence analysisAdd BLAST | 38 |
Sequence similaritiesi
Belongs to the MDM20/NAA25 family.Curated
Keywords - Domaini
Repeat, TPR repeatPhylogenomic databases
eggNOGi | KOG2053, Eukaryota |
HOGENOMi | CLU_008075_0_0_1 |
InParanoidi | Q9VDQ7 |
OMAi | ICRRMWA |
OrthoDBi | 1022953at2759 |
PhylomeDBi | Q9VDQ7 |
Family and domain databases
Gene3Di | 1.25.40.10, 1 hit |
InterProi | View protein in InterPro IPR019183, N-acetylTrfase_B_cplx_non-cat IPR013026, TPR-contain_dom IPR011990, TPR-like_helical_dom_sf |
PANTHERi | PTHR22767:SF3, PTHR22767:SF3, 1 hit |
Pfami | View protein in Pfam PF09797, NatB_MDM20, 1 hit |
SUPFAMi | SSF48452, SSF48452, 1 hit |
PROSITEi | View protein in PROSITE PS50293, TPR_REGION, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9VDQ7-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAQQQGMDSA LFERRLRPVY DNLEVGNNRK ALQESEKLLR KHPNLLCARA
60 70 80 90 100
LKGLSLLRLG RYDESHGCLQ TVAEEKPTDD STLQVLSFCY REMEQLDKIV
110 120 130 140 150
ELYQHAVKQN PGNEELLAHL FISHVRVEDY KAQQAVALQL YKAQPKNAYY
160 170 180 190 200
FWSVISVVFQ GIRGPESALP EKRKIYLGLA QRMVDKHIKE GKMETEQEAF
210 220 230 240 250
LYLHILKLQN KYQEAWEFLT GELCAKLYPG APVSMKFELL KELGNWRELN
260 270 280 290 300
ELLQQLLDAD RDRWDFYKEY IQSSFELLKL TPQGVENGDK DSLARCQEFL
310 320 330 340 350
QRIIDSSERK KRGPYLARLE LHQRMRAEQL PAEKLIGDFD EMVIEYFRLF
360 370 380 390 400
GDKSCCTHDI ALFLPSISMN QRQALASKLL LESGVTSTSL PKNKEQLQKH
410 420 430 440 450
LCALQISRMC GSHMDLPADH LLAFYTALKL HYEHGRTTFG KKLLATEMGP
460 470 480 490 500
SDAYALLAAN VMYDLSRREN KSDHLFEALC LLQYVLRNST SNFHVKLLSL
510 520 530 540 550
KIYHLFGCQV GAQEMYEYLD IKQIQLDSMG YVHCQLLALG GRFSGNRNVY
560 570 580 590 600
DATLKFFTNS YKERLEYIAL TYRFCTFSKM EEFMNFKERL TNSLQYVACS
610 620 630 640 650
VEAQICDLVS CYGNITQNLS AYVAMSIEPA EDRIAWHELS DNRDLGAIIR
660 670 680 690 700
WDPLHEVDAA EERRESFDQE VEVLQVRSLM LRLFASFIDL YHGAGLKDSP
710 720 730 740 750
AGAETATLEL LRESWTGLFQ RLRVMNYKPL AQKFLVNLLP TRLHLLLDMP
760 770 780 790 800
YERFFDDLAQ LVLDLQSGSA KLAEQCKRVG DNVIATMELC VQTINESNES
810 820 830 840 850
NGIDGLWKRR GQQQKVAASL EMLSLYAFLL SVLNDKLQVS SKTKTKKKPV
860 870 880 890 900
ENKVDGPQPI SEKERSQMLQ ELMRQLKHKF EACDTAIRSW KAPVLPRDLS
910 920 930 940
SFMSDMSLKP EVEATLIGDV SATFKDSHEL MVTELRNLLK DKIRMVAK
Sequence cautioni
The sequence AAQ22590 differs from that shown. Reason: Frameshift.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 437 | T → S in AAQ22590 (Ref. 3) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE014297 Genomic DNA Translation: AAF55733.1 BT010121 mRNA Translation: AAQ22590.1 Frameshift. |
RefSeqi | NP_650858.1, NM_142601.2 |
Genome annotation databases
EnsemblMetazoai | FBtr0083829; FBpp0083238; FBgn0243511 |
GeneIDi | 42389 |
KEGGi | dme:Dmel_CG4845 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE014297 Genomic DNA Translation: AAF55733.1 BT010121 mRNA Translation: AAQ22590.1 Frameshift. |
RefSeqi | NP_650858.1, NM_142601.2 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 67375, 14 interactors |
IntActi | Q9VDQ7, 4 interactors |
STRINGi | 7227.FBpp0083238 |
Proteomic databases
PaxDbi | Q9VDQ7 |
PRIDEi | Q9VDQ7 |
Genome annotation databases
EnsemblMetazoai | FBtr0083829; FBpp0083238; FBgn0243511 |
GeneIDi | 42389 |
KEGGi | dme:Dmel_CG4845 |
Organism-specific databases
CTDi | 42389 |
FlyBasei | FBgn0243511, psidin |
Phylogenomic databases
eggNOGi | KOG2053, Eukaryota |
HOGENOMi | CLU_008075_0_0_1 |
InParanoidi | Q9VDQ7 |
OMAi | ICRRMWA |
OrthoDBi | 1022953at2759 |
PhylomeDBi | Q9VDQ7 |
Miscellaneous databases
BioGRID-ORCSi | 42389, 0 hits in 1 CRISPR screen |
GenomeRNAii | 42389 |
PROi | PR:Q9VDQ7 |
Gene expression databases
Bgeei | FBgn0243511, Expressed in embryonic/larval hemocyte (Drosophila) and 38 other tissues |
ExpressionAtlasi | Q9VDQ7, baseline and differential |
Genevisiblei | Q9VDQ7, DM |
Family and domain databases
Gene3Di | 1.25.40.10, 1 hit |
InterProi | View protein in InterPro IPR019183, N-acetylTrfase_B_cplx_non-cat IPR013026, TPR-contain_dom IPR011990, TPR-like_helical_dom_sf |
PANTHERi | PTHR22767:SF3, PTHR22767:SF3, 1 hit |
Pfami | View protein in Pfam PF09797, NatB_MDM20, 1 hit |
SUPFAMi | SSF48452, SSF48452, 1 hit |
PROSITEi | View protein in PROSITE PS50293, TPR_REGION, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NAA25_DROME | |
Accessioni | Q9VDQ7Primary (citable) accession number: Q9VDQ7 Secondary accession number(s): Q7YTX6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 15, 2008 |
Last sequence update: | May 1, 2000 | |
Last modified: | December 2, 2020 | |
This is version 139 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - SIMILARITY comments
Index of protein domains and families