UniProtKB - Q9VCU9 (DCR1_DROME)
Protein
Endoribonuclease Dcr-1
Gene
Dcr-1
Organism
Drosophila melanogaster (Fruit fly)
Status
Functioni
Essential for RNA interference (RNAi); double-stranded RNA (dsRNA) induces potent and specific gene silencing. RNAi is mediated by the RNA-induced silencing complex (RISC), a sequence-specific, multicomponent nuclease that destroys or silences messenger RNAs homologous to the silencing trigger. May carry out the initiation step of RNAi by cleaving dsRNA to produce 22 bp dsRNAs (siRNAs) which target the selective destruction of homologous RNAs. During embryogenesis, involved in germline fate determination.3 Publications
Cofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 2032 | Magnesium or manganeseBy similarity | 1 | |
Sitei | 2132 | Important for activityBy similarity | 1 | |
Metal bindingi | 2136 | Magnesium or manganeseBy similarity | 1 | |
Metal bindingi | 2139 | Magnesium or manganeseBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 37 – 44 | ATPSequence analysis | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- bidentate ribonuclease III activity Source: FlyBase
- deoxyribonuclease I activity Source: GO_Central
- helicase activity Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
- pre-miRNA binding Source: FlyBase
- ribonuclease III activity Source: MGI
- RNA binding Source: GO_Central
- single-stranded RNA binding Source: FlyBase
GO - Biological processi
- apoptotic DNA fragmentation Source: GO_Central
- dendrite morphogenesis Source: FlyBase
- dsRNA transport Source: FlyBase
- germarium-derived female germ-line cyst formation Source: FlyBase
- germarium-derived oocyte fate determination Source: FlyBase
- germ-line stem cell division Source: FlyBase
- mitotic cell cycle, embryonic Source: FlyBase
- pole cell formation Source: FlyBase
- pre-miRNA processing Source: FlyBase
- production of miRNAs involved in gene silencing by miRNA Source: FlyBase
- production of siRNA involved in RNA interference Source: UniProtKB
- response to starvation Source: FlyBase
- RNA interference Source: FlyBase
- RNA phosphodiester bond hydrolysis Source: GO_Central
- segment polarity determination Source: FlyBase
- siRNA loading onto RISC involved in RNA interference Source: FlyBase
Keywordsi
Molecular function | Endonuclease, Helicase, Hydrolase, Nuclease, RNA-binding |
Biological process | RNA-mediated gene silencing |
Ligand | ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-DME-203927, MicroRNA (miRNA) biogenesis R-DME-426486, Small interfering RNA (siRNA) biogenesis |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Dcr-1Imported ORF Names:CG4792 |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Holometabola › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0039016, Dcr-1 |
Subcellular locationi
Other locations
- cytoplasm Source: FlyBase
- RISC complex Source: UniProtKB
- RISC-loading complex Source: FlyBase
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000180474 | 1 – 2249 | Endoribonuclease Dcr-1Add BLAST | 2249 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1423 | Phosphoserine1 Publication | 1 | |
Modified residuei | 1877 | Phosphoserine1 Publication | 1 | |
Modified residuei | 1880 | Phosphoserine1 Publication | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | Q9VCU9 |
PRIDEi | Q9VCU9 |
PTM databases
iPTMneti | Q9VCU9 |
Expressioni
Gene expression databases
Bgeei | FBgn0039016, Expressed in eye disc (Drosophila) and 34 other tissues |
ExpressionAtlasi | Q9VCU9, baseline and differential |
Genevisiblei | Q9VCU9, DM |
Interactioni
Subunit structurei
Interacts with AGO2 and Fmr1 to form the RNA-induced silencing complex (RISC), a ribonucleoprotein (RNP) complex involved in translation regulation; other components of the complex are RpL5, RpL11, AGO2 and Rm62 (PubMed:11498593, PubMed:12368261).
Interacts with piwi and vas; these interactions occur in the polar granules (PubMed:16949822).
3 PublicationsBinary interactionsi
Hide detailsProtein-protein interaction databases
BioGRIDi | 67642, 24 interactors |
DIPi | DIP-23028N |
IntActi | Q9VCU9, 11 interactors |
STRINGi | 7227.FBpp0083717 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 485 – 648 | Helicase C-terminalPROSITE-ProRule annotationAdd BLAST | 164 | |
Domaini | 825 – 920 | Dicer dsRNA-binding foldPROSITE-ProRule annotationAdd BLAST | 96 | |
Domaini | 1099 – 1246 | PAZPROSITE-ProRule annotationAdd BLAST | 148 | |
Domaini | 1698 – 1919 | RNase III 1PROSITE-ProRule annotationAdd BLAST | 222 | |
Domaini | 1993 – 2150 | RNase III 2PROSITE-ProRule annotationAdd BLAST | 158 | |
Domaini | 2175 – 2241 | DRBMPROSITE-ProRule annotationAdd BLAST | 67 |
Sequence similaritiesi
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG0701, Eukaryota |
GeneTreei | ENSGT00940000156287 |
HOGENOMi | CLU_000907_4_4_1 |
InParanoidi | Q9VCU9 |
OMAi | YLMLFDP |
OrthoDBi | 1337630at2759 |
PhylomeDBi | Q9VCU9 |
Family and domain databases
CDDi | cd10843, DSRM_DICER, 1 hit cd00593, RIBOc, 2 hits |
Gene3Di | 1.10.1520.10, 1 hit 3.30.160.380, 1 hit |
InterProi | View protein in InterPro IPR038248, Dicer_dimer_sf IPR005034, Dicer_dimerisation_dom IPR044441, DICER_DSRM IPR014720, dsRBD_dom IPR001650, Helicase_C IPR027417, P-loop_NTPase IPR003100, PAZ_dom IPR036085, PAZ_dom_sf IPR000999, RNase_III_dom IPR036389, RNase_III_sf |
Pfami | View protein in Pfam PF00271, Helicase_C, 1 hit PF02170, PAZ, 1 hit PF00636, Ribonuclease_3, 2 hits |
SMARTi | View protein in SMART SM00490, HELICc, 1 hit SM00949, PAZ, 1 hit SM00535, RIBOc, 2 hits |
SUPFAMi | SSF101690, SSF101690, 1 hit SSF52540, SSF52540, 1 hit SSF69065, SSF69065, 2 hits |
PROSITEi | View protein in PROSITE PS51327, DICER_DSRBF, 1 hit PS50137, DS_RBD, 1 hit PS51194, HELICASE_CTER, 1 hit PS50821, PAZ, 1 hit PS00517, RNASE_3_1, 1 hit PS50142, RNASE_3_2, 2 hits |
i Sequence
Sequence statusi: Complete.
Q9VCU9-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAFHWCDNNL HTTVFTPRDF QVELLATAYE RNTIICLGHR SSKEFIALKL
60 70 80 90 100
LQELSRRARR HGRVSVYLSC EVGTSTEPCS IYTMLTHLTD LRVWQEQPDM
110 120 130 140 150
QIPFDHCWTD YHVSILRPEG FLYLLETREL LLSSVELIVL EDCHDSAVYQ
160 170 180 190 200
RIRPLFENHI MPAPPADRPR ILGLAGPLHS AGCELQQLSA MLATLEQSVL
210 220 230 240 250
CQIETASDIV TVLRYCSRPH EYIVQCAPFE MDELSLVLAD VLNTHKSFLL
260 270 280 290 300
DHRYDPYEIY GTDQFMDELK DIPDPKVDPL NVINSLLVVL HEMGPWCTQR
310 320 330 340 350
AAHHFYQCNE KLKVKTPHER HYLLYCLVST ALIQLYSLCE HAFHRHLGSG
360 370 380 390 400
SDSRQTIERY SSPKVRRLLQ TLRCFKPEEV HTQADGLRRM RHQVDQADFN
410 420 430 440 450
RLSHTLESKC RMVDQMDQPP TETRALVATL EQILHTTEDR QTNRSAARVT
460 470 480 490 500
PTPTPAHAKP KPSSGANTAQ PRTRRRVYTR RHHRDHNDGS DTLCALIYCN
510 520 530 540 550
QNHTARVLFE LLAEISRRDP DLKFLRCQYT TDRVADPTTE PKEAELEHRR
560 570 580 590 600
QEEVLKRFRM HDCNVLIGTS VLEEGIDVPK CNLVVRWDPP TTYRSYVQCK
610 620 630 640 650
GRARAAPAYH VILVAPSYKS PTVGSVQLTD RSHRYICATG DTTEADSDSD
660 670 680 690 700
DSAMPNSSGS DPYTFGTARG TVKILNPEVF SKQPPTACDI KLQEIQDELP
710 720 730 740 750
AAAQLDTSNS SDEAVSMSNT SPSESSTEQK SRRFQCELSS LTEPEDTSDT
760 770 780 790 800
TAEIDTAHSL ASTTKDLVHQ MAQYREIEQM LLSKCANTEP PEQEQSEAER
810 820 830 840 850
FSACLAAYRP KPHLLTGASV DLGSAIALVN KYCARLPSDT FTKLTALWRC
860 870 880 890 900
TRNERAGVTL FQYTLRLPIN SPLKHDIVGL PMPTQTLARR LAALQACVEL
910 920 930 940 950
HRIGELDDQL QPIGKEGFRA LEPDWECFEL EPEDEQIVQL SDEPRPGTTK
960 970 980 990 1000
RRQYYYKRIA SEFCDCRPVA GAPCYLYFIQ LTLQCPIPEE QNTRGRKIYP
1010 1020 1030 1040 1050
PEDAQQGFGI LTTKRIPKLS AFSIFTRSGE VKVSLELAKE RVILTSEQIV
1060 1070 1080 1090 1100
CINGFLNYTF TNVLRLQKFL MLFDPDSTEN CVFIVPTVKA PAGGKHIDWQ
1110 1120 1130 1140 1150
FLELIQANGN TMPRAVPDEE RQAQPFDPQR FQDAVVMPWY RNQDQPQYFY
1160 1170 1180 1190 1200
VAEICPHLSP LSCFPGDNYR TFKHYYLVKY GLTIQNTSQP LLDVDHTSAR
1210 1220 1230 1240 1250
LNFLTPRYVN RKGVALPTSS EETKRAKREN LEQKQILVPE LCTVHPFPAS
1260 1270 1280 1290 1300
LWRTAVCLPC ILYRINGLLL ADDIRKQVSA DLGLGRQQIE DEDFEWPMLD
1310 1320 1330 1340 1350
FGWSLSEVLK KSRESKQKES LKDDTINGKD LADVEKKPTS EETQLDKDSK
1360 1370 1380 1390 1400
DDKVEKSAIE LIIEGEEKLQ EADDFIEIGT WSNDMADDIA SFNQEDDDED
1410 1420 1430 1440 1450
DAFHLPVLPA NVKFCDQQTR YGSPTFWDVS NGESGFKGPK SSQNKQGGKG
1460 1470 1480 1490 1500
KAKGPAKPTF NYYDSDNSLG SSYDDDDNAG PLNYMHHNYS SDDDDVADDI
1510 1520 1530 1540 1550
DAGRIAFTSK NEAETIETAQ EVEKRQKQLS IIQATNANER QYQQTKNLLI
1560 1570 1580 1590 1600
GFNFKHEDQK EPATIRYEES IAKLKTEIES GGMLVPHDQQ LVLKRSDAAE
1610 1620 1630 1640 1650
AQVAKVSMME LLKQLLPYVN EDVLAKKLGD RRELLLSDLV ELNADWVARH
1660 1670 1680 1690 1700
EQETYNVMGC GDSFDNYNDH HRLNLDEKQL KLQYERIEIE PPTSTKAITS
1710 1720 1730 1740 1750
AILPAGFSFD RQPDLVGHPG PSPSIILQAL TMSNANDGIN LERLETIGDS
1760 1770 1780 1790 1800
FLKYAITTYL YITYENVHEG KLSHLRSKQV ANLNLYRLGR RKRLGEYMIA
1810 1820 1830 1840 1850
TKFEPHDNWL PPCYYVPKEL EKALIEAKIP THHWKLADLL DIKNLSSVQI
1860 1870 1880 1890 1900
CEMVREKADA LGLEQNGGAQ NGQLDDSNDS CNDFSCFIPY NLVSQHSIPD
1910 1920 1930 1940 1950
KSIADCVEAL IGAYLIECGP RGALLFMAWL GVRVLPITRQ LDGGNQEQRI
1960 1970 1980 1990 2000
PGSTKPNAEN VVTVYGAWPT PRSPLLHFAP NATEELDQLL SGFEEFEESL
2010 2020 2030 2040 2050
GYKFRDRSYL LQAMTHASYT PNRLTDCYQR LEFLGDAVLD YLITRHLYED
2060 2070 2080 2090 2100
PRQHSPGALT DLRSALVNNT IFASLAVRHG FHKFFRHLSP GLNDVIDRFV
2110 2120 2130 2140 2150
RIQQENGHCI SEEYYLLSEE ECDDAEDVEV PKALGDVFES IAGAIFLDSN
2160 2170 2180 2190 2200
MSLDVVWHVY SNMMSPEIEQ FSNSVPKSPI RELLELEPET AKFGKPEKLA
2210 2220 2230 2240
DGRRVRVTVD VFCKGTFRGI GRNYRIAKCT AAKCALRQLK KQGLIAKKD
Sequence cautioni
The sequence AAK84929 differs from that shown. Reason: Erroneous initiation.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 1338 – 1339 | PT → AI (PubMed:12537569).Curated | 2 | |
Sequence conflicti | 1345 | L → I (PubMed:12537569).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE014297 Genomic DNA Translation: AAF56056.1 AY050230 mRNA Translation: AAK84929.1 Different initiation. |
RefSeqi | NP_524453.1, NM_079729.3 |
Genome annotation databases
EnsemblMetazoai | FBtr0084324; FBpp0083717; FBgn0039016 |
GeneIDi | 42693 |
KEGGi | dme:Dmel_CG4792 |
UCSCi | CG4792-RA, d. melanogaster |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE014297 Genomic DNA Translation: AAF56056.1 AY050230 mRNA Translation: AAK84929.1 Different initiation. |
RefSeqi | NP_524453.1, NM_079729.3 |
3D structure databases
SMRi | Q9VCU9 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 67642, 24 interactors |
DIPi | DIP-23028N |
IntActi | Q9VCU9, 11 interactors |
STRINGi | 7227.FBpp0083717 |
PTM databases
iPTMneti | Q9VCU9 |
Proteomic databases
PaxDbi | Q9VCU9 |
PRIDEi | Q9VCU9 |
Genome annotation databases
EnsemblMetazoai | FBtr0084324; FBpp0083717; FBgn0039016 |
GeneIDi | 42693 |
KEGGi | dme:Dmel_CG4792 |
UCSCi | CG4792-RA, d. melanogaster |
Organism-specific databases
CTDi | 42693 |
FlyBasei | FBgn0039016, Dcr-1 |
Phylogenomic databases
eggNOGi | KOG0701, Eukaryota |
GeneTreei | ENSGT00940000156287 |
HOGENOMi | CLU_000907_4_4_1 |
InParanoidi | Q9VCU9 |
OMAi | YLMLFDP |
OrthoDBi | 1337630at2759 |
PhylomeDBi | Q9VCU9 |
Enzyme and pathway databases
Reactomei | R-DME-203927, MicroRNA (miRNA) biogenesis R-DME-426486, Small interfering RNA (siRNA) biogenesis |
Miscellaneous databases
BioGRID-ORCSi | 42693, 0 hits in 1 CRISPR screen |
GenomeRNAii | 42693 |
PROi | PR:Q9VCU9 |
Gene expression databases
Bgeei | FBgn0039016, Expressed in eye disc (Drosophila) and 34 other tissues |
ExpressionAtlasi | Q9VCU9, baseline and differential |
Genevisiblei | Q9VCU9, DM |
Family and domain databases
CDDi | cd10843, DSRM_DICER, 1 hit cd00593, RIBOc, 2 hits |
Gene3Di | 1.10.1520.10, 1 hit 3.30.160.380, 1 hit |
InterProi | View protein in InterPro IPR038248, Dicer_dimer_sf IPR005034, Dicer_dimerisation_dom IPR044441, DICER_DSRM IPR014720, dsRBD_dom IPR001650, Helicase_C IPR027417, P-loop_NTPase IPR003100, PAZ_dom IPR036085, PAZ_dom_sf IPR000999, RNase_III_dom IPR036389, RNase_III_sf |
Pfami | View protein in Pfam PF00271, Helicase_C, 1 hit PF02170, PAZ, 1 hit PF00636, Ribonuclease_3, 2 hits |
SMARTi | View protein in SMART SM00490, HELICc, 1 hit SM00949, PAZ, 1 hit SM00535, RIBOc, 2 hits |
SUPFAMi | SSF101690, SSF101690, 1 hit SSF52540, SSF52540, 1 hit SSF69065, SSF69065, 2 hits |
PROSITEi | View protein in PROSITE PS51327, DICER_DSRBF, 1 hit PS50137, DS_RBD, 1 hit PS51194, HELICASE_CTER, 1 hit PS50821, PAZ, 1 hit PS00517, RNASE_3_1, 1 hit PS50142, RNASE_3_2, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DCR1_DROME | |
Accessioni | Q9VCU9Primary (citable) accession number: Q9VCU9 Secondary accession number(s): Q961S7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 16, 2004 |
Last sequence update: | May 1, 2000 | |
Last modified: | April 7, 2021 | |
This is version 162 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - SIMILARITY comments
Index of protein domains and families