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Protein

Proteoglycan Cow

Gene

Cow

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Binds to the Wnt signaling protein wg, stabilizes it and promotes its extracellular distribution. This is required for establishment of a wg gradient during development to allow for regulation of target genes at different levels.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei233 – 234Reactive bondPROSITE-ProRule annotation2

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi481 – 492PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

  • calcium ion binding Source: InterPro
  • serine-type endopeptidase inhibitor activity Source: UniProtKB-KW
  • Wnt-protein binding Source: FlyBase

GO - Biological processi

  • establishment of protein localization to extracellular region Source: FlyBase
  • extracellular transport Source: FlyBase
  • negative regulation of canonical Wnt signaling pathway Source: FlyBase
  • positive regulation of canonical Wnt signaling pathway Source: FlyBase
  • segment polarity determination Source: FlyBase
  • wing disc dorsal/ventral pattern formation Source: FlyBase

Keywordsi

Molecular functionProtease inhibitor, Serine protease inhibitor
LigandCalcium

Names & Taxonomyi

Protein namesi
Recommended name:
Proteoglycan CowCurated
Alternative name(s):
Carrier of wingless1 Publication
Short name:
Carrier of wg1 Publication
Gene namesi
Name:CowImported
ORF Names:CG13830Imported
OrganismiDrosophila melanogaster (Fruit fly)Imported
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0039054 Cow

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Disruption phenotypei

Predominantly embryonic lethal with 50% of embryos dying before cuticle formation and 10% showing a weak denticle belt fusion phenotype. RNAi-mediated knockdown in the developing wing results in development of ectopic chemosensory bristles along or near the wing margin on both the anterior and posterior sides.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi287 – 288SG → AA: Abolishes interaction with wg and causes significant reduction of 100 kDa form which is likely to be the heparan sulfate-modified form; when associated with A-331 and A-332. 1 Publication2
Mutagenesisi331 – 332SG → AA: Abolishes interaction with wg and causes significant reduction of 100 kDa form which is likely to be the heparan sulfate-modified form; when associated with A-287 and A-288. 1 Publication2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 27Sequence analysisAdd BLAST27
ChainiPRO_500721623028 – 629Proteoglycan CowSequence analysisAdd BLAST602

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi142N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Disulfide bondi228 ↔ 258PROSITE-ProRule annotation
Disulfide bondi231 ↔ 251PROSITE-ProRule annotation
Disulfide bondi240 ↔ 272PROSITE-ProRule annotation
Glycosylationi244N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Disulfide bondi536 ↔ 555PROSITE-ProRule annotation
Disulfide bondi566 ↔ 573PROSITE-ProRule annotation
Disulfide bondi575 ↔ 594PROSITE-ProRule annotation

Post-translational modificationi

Contains heparan sulfate O-linked oligosaccharides.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Heparan sulfate, Proteoglycan

Proteomic databases

PaxDbiQ9VCR3
PRIDEiQ9VCR3

Expressioni

Tissue specificityi

In the wing disk, detected throughout the disk where it is localized primarily to the apical surface but is also present at the basal surface (at protein level).1 Publication

Gene expression databases

BgeeiFBgn0039054
ExpressionAtlasiQ9VCR3 baseline and differential

Interactioni

Subunit structurei

Interacts (in heparan sulfate-bound form) with wg.1 Publication

GO - Molecular functioni

  • Wnt-protein binding Source: FlyBase

Protein-protein interaction databases

IntActiQ9VCR3, 6 interactors
STRINGi7227.FBpp0291233

Structurei

3D structure databases

ProteinModelPortaliQ9VCR3
SMRiQ9VCR3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini222 – 273Kazal-likePROSITE-ProRule annotationAdd BLAST52
Domaini468 – 503EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini508 – 535EF-hand 2PROSITE-ProRule annotationAdd BLAST28
Domaini533 – 594Thyroglobulin type-1PROSITE-ProRule annotationAdd BLAST62

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi301 – 317Gln-richPROSITE-ProRule annotationAdd BLAST17
Compositional biasi323 – 360Asn-richPROSITE-ProRule annotationAdd BLAST38

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiKOG3555 Eukaryota
ENOG410Y6A8 LUCA
GeneTreeiENSGT00510000046429
OMAiFCPCKEN
OrthoDBiEOG091G0LMB
PhylomeDBiQ9VCR3

Family and domain databases

CDDicd00191 TY, 1 hit
Gene3Di4.10.800.10, 1 hit
InterProiView protein in InterPro
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR002350 Kazal_dom
IPR036058 Kazal_dom_sf
IPR019577 SPARC/Testican_Ca-bd-dom
IPR000716 Thyroglobulin_1
IPR036857 Thyroglobulin_1_sf
PfamiView protein in Pfam
PF07648 Kazal_2, 1 hit
PF10591 SPARC_Ca_bdg, 1 hit
PF00086 Thyroglobulin_1, 1 hit
SMARTiView protein in SMART
SM00280 KAZAL, 1 hit
SM00211 TY, 1 hit
SUPFAMiSSF100895 SSF100895, 1 hit
SSF47473 SSF47473, 1 hit
SSF57610 SSF57610, 1 hit
PROSITEiView protein in PROSITE
PS00018 EF_HAND_1, 1 hit
PS51465 KAZAL_2, 1 hit
PS00484 THYROGLOBULIN_1_1, 1 hit
PS51162 THYROGLOBULIN_1_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9VCR3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKHSPLIASA CLALVLMSSS LIGSTEARNK KKYVGETGGD FEFIDEINKN
60 70 80 90 100
TQSNKNLGEH KRWIHDPSSD LCRPLNCKKR EICLLEDEFS AVCVSKKELH
110 120 130 140 150
KNRDEIITKA KYLEEEAKRR VNQQDNQDSQ DAEDINNDDE DNSSDGGSSN
160 170 180 190 200
SSPTGTNNAQ ASVQGNEETD DEDKSLSLGD DDESKEDDVF YENSIAAGDK
210 220 230 240 250
QQQQQQLSQP AAGPSVIQQD DDEELDNCKP CPVAKPTFLC GADNRTYSSL
260 270 280 290 300
CRLDYHNCIH STSIRIACKG FCPCKEIVDG KRLQRISGYN NKYNKKISLD
310 320 330 340 350
QQQQQQQQQQ QQQQQQQAYK DSNNNNIMMN SGNIMGGNNN DFNTIMNDKE
360 370 380 390 400
DNNRHNNHIN AQYTFTPEEI KYDNKHYKYL KYTAYKKDSK YQEDKHKMRN
410 420 430 440 450
YNEVVEKQQQ KFNKNSNLNA YPSKSAECKP QQLTAIGNRL LDWFSVIMAD
460 470 480 490 500
SKKRRQHSQK SKAHFPPACK TEAKWMFGHL DLNNDGQLSL QEMYDLEHDQ
510 520 530 540 550
NERCIKPFID TCDLDTDSSI NTREWCRCFE KTDRPCAAVR RRIAGDFAGA
560 570 580 590 600
YAPDCDIQGF YKPTQCHNSV GVCWCVDKHG VEFANTRTRG KPNCESVVNN
610 620
AASLTSDDED EGADDEDSAE GSADQMLVF
Length:629
Mass (Da):71,474
Last modified:November 30, 2010 - v3
Checksum:iFB8B3537D41FB17A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014297 Genomic DNA Translation: AAF56093.3
AE014297 Genomic DNA Translation: ACZ94990.1
BT044452 mRNA Translation: ACH92517.1
RefSeqiNP_001097883.2, NM_001104413.3
NP_001163695.1, NM_001170224.2
UniGeneiDm.16579

Genome annotation databases

EnsemblMetazoaiFBtr0302022; FBpp0291232; FBgn0039054
FBtr0302023; FBpp0291233; FBgn0039054
GeneIDi42733
KEGGidme:Dmel_CG13830
UCSCiCG13830-RB d. melanogaster

Similar proteinsi

Entry informationi

Entry nameiCOW_DROME
AccessioniPrimary (citable) accession number: Q9VCR3
Secondary accession number(s): B5RJH8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 7, 2016
Last sequence update: November 30, 2010
Last modified: July 18, 2018
This is version 159 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase

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