UniProtKB - Q9VCP0 (ELOA1_DROME)
Protein
Transcription elongation factor B polypeptide 3
Gene
EloA
Organism
Drosophila melanogaster (Fruit fly)
Status
Functioni
SIII, also known as elongin, is a general transcription elongation factor that increases the RNA polymerase II transcription elongation past template-encoded arresting sites. Subunit A is transcriptionally active and its transcription activity is strongly enhanced by binding to the dimeric complex of the SIII regulatory subunits B and C (elongin BC complex). May play an important role in metamorphosis.1 Publication
GO - Biological processi
- imaginal disc-derived wing vein specification Source: FlyBase
- positive regulation of transcription elongation from RNA polymerase II promoter Source: FlyBase
- transcription elongation from RNA polymerase II promoter Source: UniProtKB
Keywordsi
Molecular function | Developmental protein |
Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
Reactomei | R-DME-112382, Formation of RNA Pol II elongation complex R-DME-674695, RNA Polymerase II Pre-transcription Events R-DME-6796648, TP53 Regulates Transcription of DNA Repair Genes R-DME-75955, RNA Polymerase II Transcription Elongation |
Names & Taxonomyi
Protein namesi | Recommended name: Transcription elongation factor B polypeptide 3Alternative name(s): Elongin-A RNA polymerase II transcription factor SIII subunit A dEloA |
Gene namesi | Name:EloA ORF Names:CG6755 |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Holometabola › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0039066, EloA |
Subcellular locationi
Nucleus
- Nucleus PROSITE-ProRule annotation
Nucleus
- elongin complex Source: FlyBase
- nucleus Source: FlyBase
- transcription elongation factor complex Source: UniProtKB
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000086959 | 1 – 643 | Transcription elongation factor B polypeptide 3Add BLAST | 643 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 120 | Phosphoserine1 Publication | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | Q9VCP0 |
PRIDEi | Q9VCP0 |
PTM databases
iPTMneti | Q9VCP0 |
Expressioni
Developmental stagei
Expression at low levels during embryonic expression and peaks during mid larval stages.1 Publication
Gene expression databases
Bgeei | FBgn0039066, Expressed in embryo and 49 other tissues |
ExpressionAtlasi | Q9VCP0, baseline and differential |
Genevisiblei | Q9VCP0, DM |
Interactioni
Protein-protein interaction databases
BioGRIDi | 67692, 15 interactors |
IntActi | Q9VCP0, 5 interactors |
STRINGi | 7227.FBpp0083766 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 9 – 82 | TFIIS N-terminalPROSITE-ProRule annotationAdd BLAST | 74 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 413 – 571 | Activation domainBy similarityAdd BLAST | 159 | |
Regioni | 439 – 448 | Interacting with Elongin BC complexBy similarity | 10 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 118 – 348 | Lys/Ser-richAdd BLAST | 231 |
Domaini
The elongin BC complex binding domain is also known as BC-box with the consensus [APST]-L-x3-C-x3-[AILV].By similarity
Phylogenomic databases
eggNOGi | KOG2821, Eukaryota |
GeneTreei | ENSGT00390000002428 |
HOGENOMi | CLU_021679_1_0_1 |
InParanoidi | Q9VCP0 |
OMAi | SNQCFTE |
PhylomeDBi | Q9VCP0 |
Family and domain databases
Gene3Di | 1.20.930.10, 1 hit |
InterProi | View protein in InterPro IPR010684, RNA_pol_II_trans_fac_SIII_A IPR003617, TFIIS/CRSP70_N_sub IPR035441, TFIIS/LEDGF_dom_sf IPR017923, TFIIS_N |
Pfami | View protein in Pfam PF06881, Elongin_A, 1 hit PF08711, Med26, 1 hit |
SMARTi | View protein in SMART SM00509, TFS2N, 1 hit |
SUPFAMi | SSF47676, SSF47676, 1 hit |
PROSITEi | View protein in PROSITE PS51319, TFIIS_N, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketIsoform A1 Publication (identifier: Q9VCP0-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MASTSNLLDV VRHYQRSIEK HGEDEQRLLH CITKLFNLPI KFEHLQETGI
60 70 80 90 100
GKTVNALRKI SGEVGVAAKT LVTKWKAMVA KEDPSIASTP TAIHNEEDSG
110 120 130 140 150
KTKSSDEDPD QENKGGNSSS GEDLNTSKHK SKHAKSTKHE RSSSSRSHSK
160 170 180 190 200
SRSDSDKKHK SSRHDKSKDR DKDREGQKEA KEHKEKKSNG EHKSKDSSKS
210 220 230 240 250
SSSHKSSKSE SHKSEHTKSK HEKDKTSHSE LKEVKDKSSK HKSSSSKSSK
260 270 280 290 300
RSHSPPRHEE ESQKAKIPKV KSKSEEDSAD GFDSSMGANF DDVLGLLNIP
310 320 330 340 350
ISSKKSSSNS KSKFVAKPTA APSSSALSAP TTAGSSKEAL STSSRPTSKK
360 370 380 390 400
PELLASTAKL EPLDPNIALE LPTISNNYKP MPLNQTVMDV VFNQGGSHKA
410 420 430 440 450
QASRYFNESE ALAQGISSKT MRTKIYSGVR TGQILQVPSL FDLCTRVLQK
460 470 480 490 500
NIDALEYTGG VPFEVLRPVL ERATPQQLLN FEEYNPYLMD DSDVLWQQHV
510 520 530 540 550
QRHCRSQRRE EMETWREMFL RCQEEKDRKL SILAESIKAS QKISEAPVRK
560 570 580 590 600
TQLAFVDSMV KPPRSVQRKQ EQYGTKGKLI ATPAARVAAL SSVTPNAAKV
610 620 630 640
GDARLRVLAA ARDTAQVGAG PARSKKAPLM AKTLQFMRGR LKR
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 485 | N → D in AAO41456 (Ref. 4) Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_050782 | 1 – 77 | Missing in isoform B. 2 PublicationsAdd BLAST | 77 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE014297 Genomic DNA Translation: AAF56117.1 AE014297 Genomic DNA Translation: AAN13934.1 AY061363 mRNA Translation: AAL28911.1 BT003775 mRNA Translation: AAO41456.1 |
RefSeqi | NP_651135.1, NM_142878.4 [Q9VCP0-2] NP_732846.1, NM_170061.3 [Q9VCP0-1] |
Genome annotation databases
EnsemblMetazoai | FBtr0084374; FBpp0083766; FBgn0039066 [Q9VCP0-1] FBtr0084375; FBpp0083767; FBgn0039066 [Q9VCP0-2] |
GeneIDi | 42749 |
KEGGi | dme:Dmel_CG6755 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE014297 Genomic DNA Translation: AAF56117.1 AE014297 Genomic DNA Translation: AAN13934.1 AY061363 mRNA Translation: AAL28911.1 BT003775 mRNA Translation: AAO41456.1 |
RefSeqi | NP_651135.1, NM_142878.4 [Q9VCP0-2] NP_732846.1, NM_170061.3 [Q9VCP0-1] |
3D structure databases
SMRi | Q9VCP0 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 67692, 15 interactors |
IntActi | Q9VCP0, 5 interactors |
STRINGi | 7227.FBpp0083766 |
PTM databases
iPTMneti | Q9VCP0 |
Proteomic databases
PaxDbi | Q9VCP0 |
PRIDEi | Q9VCP0 |
Genome annotation databases
EnsemblMetazoai | FBtr0084374; FBpp0083766; FBgn0039066 [Q9VCP0-1] FBtr0084375; FBpp0083767; FBgn0039066 [Q9VCP0-2] |
GeneIDi | 42749 |
KEGGi | dme:Dmel_CG6755 |
Organism-specific databases
CTDi | 6924 |
FlyBasei | FBgn0039066, EloA |
Phylogenomic databases
eggNOGi | KOG2821, Eukaryota |
GeneTreei | ENSGT00390000002428 |
HOGENOMi | CLU_021679_1_0_1 |
InParanoidi | Q9VCP0 |
OMAi | SNQCFTE |
PhylomeDBi | Q9VCP0 |
Enzyme and pathway databases
Reactomei | R-DME-112382, Formation of RNA Pol II elongation complex R-DME-674695, RNA Polymerase II Pre-transcription Events R-DME-6796648, TP53 Regulates Transcription of DNA Repair Genes R-DME-75955, RNA Polymerase II Transcription Elongation |
Miscellaneous databases
BioGRID-ORCSi | 42749, 0 hits in 3 CRISPR screens |
GenomeRNAii | 42749 |
PROi | PR:Q9VCP0 |
Gene expression databases
Bgeei | FBgn0039066, Expressed in embryo and 49 other tissues |
ExpressionAtlasi | Q9VCP0, baseline and differential |
Genevisiblei | Q9VCP0, DM |
Family and domain databases
Gene3Di | 1.20.930.10, 1 hit |
InterProi | View protein in InterPro IPR010684, RNA_pol_II_trans_fac_SIII_A IPR003617, TFIIS/CRSP70_N_sub IPR035441, TFIIS/LEDGF_dom_sf IPR017923, TFIIS_N |
Pfami | View protein in Pfam PF06881, Elongin_A, 1 hit PF08711, Med26, 1 hit |
SMARTi | View protein in SMART SM00509, TFS2N, 1 hit |
SUPFAMi | SSF47676, SSF47676, 1 hit |
PROSITEi | View protein in PROSITE PS51319, TFIIS_N, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ELOA1_DROME | |
Accessioni | Q9VCP0Primary (citable) accession number: Q9VCP0 Secondary accession number(s): Q86NR0, Q95RH9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 16, 2004 |
Last sequence update: | May 1, 2000 | |
Last modified: | December 2, 2020 | |
This is version 153 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase