UniProtKB - Q9VAC5 (ADA17_DROME)
Protein
ADAM 17-like protease
Gene
Tace
Organism
Drosophila melanogaster (Fruit fly)
Status
Functioni
Cofactori
Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 183 | Zinc; in inhibited formBy similarity | 1 | |
Metal bindingi | 399 | Zinc; catalyticBy similarity | 1 | |
Active sitei | 400 | PROSITE-ProRule annotation | 1 | |
Metal bindingi | 403 | Zinc; catalyticBy similarity | 1 | |
Metal bindingi | 409 | Zinc; catalyticBy similarity | 1 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- metalloendopeptidase activity Source: FlyBase
GO - Biological processi
- membrane protein ectodomain proteolysis Source: FlyBase
- negative regulation of multicellular organism growth Source: FlyBase
- negative regulation of reactive oxygen species biosynthetic process Source: FlyBase
- Notch receptor processing Source: FlyBase
- Notch signaling pathway Source: FlyBase
- positive regulation of cell death Source: FlyBase
- tumor necrosis factor-mediated signaling pathway Source: FlyBase
Keywordsi
Molecular function | Hydrolase, Metalloprotease, Protease |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
SignaLinki | Q9VAC5 |
Protein family/group databases
MEROPSi | M12.217 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Tace ORF Names:CG7908 |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Holometabola › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0039734, Tace |
Subcellular locationi
Other locations
- Membrane Curated; Single-pass type I membrane protein Curated
Plasma Membrane
- plasma membrane Source: GO_Central
Other locations
- integral component of membrane Source: FlyBase
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 212 – 662 | ExtracellularSequence analysisAdd BLAST | 451 | |
Transmembranei | 663 – 683 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 684 – 732 | CytoplasmicSequence analysisAdd BLAST | 49 |
Keywords - Cellular componenti
MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 28 | Sequence analysisAdd BLAST | 28 | |
PropeptideiPRO_0000029094 | 29 – 211 | By similarityAdd BLAST | 183 | |
ChainiPRO_0000029095 | 212 – 732 | ADAM 17-like proteaseAdd BLAST | 521 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 100 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 225 ↔ 328 | By similarity | ||
Glycosylationi | 263 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 360 ↔ 462 | By similarity | ||
Disulfide bondi | 416 ↔ 446 | By similarity | ||
Glycosylationi | 492 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 530 ↔ 551 | By similarity | ||
Disulfide bondi | 569 ↔ 578 | By similarity | ||
Disulfide bondi | 574 ↔ 586 | By similarity | ||
Disulfide bondi | 588 ↔ 595 | By similarity | ||
Glycosylationi | 604 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, ZymogenProteomic databases
PaxDbi | Q9VAC5 |
PRIDEi | Q9VAC5 |
Expressioni
Gene expression databases
Bgeei | FBgn0039734, Expressed in central nervous system and 21 other tissues |
ExpressionAtlasi | Q9VAC5, baseline and differential |
Genevisiblei | Q9VAC5, DM |
Interactioni
Protein-protein interaction databases
BioGRIDi | 68414, 2 interactors |
STRINGi | 7227.FBpp0084917 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 223 – 467 | Peptidase M12BPROSITE-ProRule annotationAdd BLAST | 245 | |
Domaini | 468 – 559 | DisintegrinPROSITE-ProRule annotationAdd BLAST | 92 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 598 – 662 | Crambin-likeAdd BLAST | 65 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 181 – 188 | Cysteine switchBy similarity | 8 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 560 – 599 | Cys-richAdd BLAST | 40 |
Domaini
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Keywords - Domaini
Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3658, Eukaryota |
GeneTreei | ENSGT00940000155443 |
HOGENOMi | CLU_004602_2_0_1 |
InParanoidi | Q9VAC5 |
OMAi | FYHGRVF |
PhylomeDBi | Q9VAC5 |
Family and domain databases
CDDi | cd14246, ADAM17_MPD, 1 hit cd04270, ZnMc_TACE_like, 1 hit |
Gene3Di | 3.40.390.10, 1 hit 4.10.70.10, 1 hit |
InterProi | View protein in InterPro IPR034025, ADAM10_ADAM17 IPR032029, ADAM17_MPD IPR001762, Disintegrin_dom IPR036436, Disintegrin_dom_sf IPR024079, MetalloPept_cat_dom_sf IPR001590, Peptidase_M12B IPR002870, Peptidase_M12B_N |
Pfami | View protein in Pfam PF16698, ADAM17_MPD, 1 hit PF00200, Disintegrin, 1 hit PF01562, Pep_M12B_propep, 1 hit |
SMARTi | View protein in SMART SM00050, DISIN, 1 hit |
SUPFAMi | SSF57552, SSF57552, 1 hit |
PROSITEi | View protein in PROSITE PS50215, ADAM_MEPRO, 1 hit PS50214, DISINTEGRIN_2, 1 hit PS00142, ZINC_PROTEASE, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketIsoform A (identifier: Q9VAC5-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MFTKCISCCG LAIISVFFAC LFVENCAALQ KTLRHYEIFH KDDVVHRVVK
60 70 80 90 100
RGAKHSTNPF NTIKEVEFTT LGKNFRLILH PHRDVLHSKF RAYAVDADGN
110 120 130 140 150
ETVVHMDHDS FYSGRVFGEL ESSVRAHIED GTMTMSIHLP EETYHIEPSW
160 170 180 190 200
RHLPEAKKDT MVAYKASDVK VHKNEAGATP KTCGYIKEGL ELEDKEHGDT
210 220 230 240 250
LDNELHTREK RQSDQYEYTP TKTRCPLLLV ADYRFFQEMG GGNTKTTINY
260 270 280 290 300
LISLIDRVHK IYNDTVWQDR SDQEGFKGMG FVIKKIVVHS EPTRLRGGEA
310 320 330 340 350
HYNMIREKWD VRNLLEVFSR EYSHKDFCLA HLFTDLKFEG GILGLAYVGS
360 370 380 390 400
PRRNSVGGIC TPEYFKNGYT LYLNSGLSSS RNHYGQRVIT READLVTAHE
410 420 430 440 450
FGHNWGSEHD PDIPECSPSA SQGGSFLMYT YSVSGYDVNN KKFSPCSLRS
460 470 480 490 500
IRKVLQAKSG RCFSEPEESF CGNLRVEGDE QCDAGLLGTE DNDSCCDKNC
510 520 530 540 550
KLRRNQGAMC SDKNSPCCQN CQFMASGMKC REAQYATCEQ EARCTGAHAE
560 570 580 590 600
CPKSPAMADG TTCQERGQCR NGKCVPYCET QGLQSCMCDI IADACKRCCR
610 620 630 640 650
MSINETCFPV EPPDVLPDGT PCITGFCNKG VCEKTIQDVV ERFWDIIEEI
660 670 680 690 700
NVAKTLRFLK DNIVMAVVLV TAVFWIPISC VISYFDRKKL RHEMKLIEWS
710 720 730
QKLDLIHPSD ERRRVIHIRV PRQKISVARA CN
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 208 | R → K in AAS48650 (Ref. 2) Curated | 1 | |
Sequence conflicti | 428 – 431 | MYTY → IHT in AAS48650 (Ref. 2) Curated | 4 | |
Sequence conflicti | 449 | R → A in AAS48650 (Ref. 2) Curated | 1 | |
Sequence conflicti | 463 | F → L in AAL28578 (Ref. 5) Curated | 1 | |
Sequence conflicti | 554 | S → F in AAS48650 (Ref. 2) Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_037658 | 252 – 262 | ISLIDRVHKIY → VSFLKNPRSII in isoform B. 1 PublicationAdd BLAST | 11 | |
Alternative sequenceiVSP_037659 | 263 – 732 | Missing in isoform B. 1 PublicationAdd BLAST | 470 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY212802 mRNA Translation: AAO53296.1 AY525768 mRNA Translation: AAS48650.1 AE014297 Genomic DNA Translation: AAN14205.3 AE014297 Genomic DNA Translation: AAF56986.2 AY061030 mRNA Translation: AAL28578.1 BT003184 mRNA Translation: AAO24939.1 Sequence problems. |
RefSeqi | NP_651759.4, NM_143502.4 [Q9VAC5-2] NP_733334.1, NM_170455.3 [Q9VAC5-1] |
Genome annotation databases
EnsemblMetazoai | FBtr0085550; FBpp0084916; FBgn0039734 [Q9VAC5-2] FBtr0085551; FBpp0084917; FBgn0039734 [Q9VAC5-1] |
GeneIDi | 43558 |
KEGGi | dme:Dmel_CG7908 |
UCSCi | CG7908-RA, d. melanogaster [Q9VAC5-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY212802 mRNA Translation: AAO53296.1 AY525768 mRNA Translation: AAS48650.1 AE014297 Genomic DNA Translation: AAN14205.3 AE014297 Genomic DNA Translation: AAF56986.2 AY061030 mRNA Translation: AAL28578.1 BT003184 mRNA Translation: AAO24939.1 Sequence problems. |
RefSeqi | NP_651759.4, NM_143502.4 [Q9VAC5-2] NP_733334.1, NM_170455.3 [Q9VAC5-1] |
3D structure databases
SMRi | Q9VAC5 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 68414, 2 interactors |
STRINGi | 7227.FBpp0084917 |
Protein family/group databases
MEROPSi | M12.217 |
Proteomic databases
PaxDbi | Q9VAC5 |
PRIDEi | Q9VAC5 |
Genome annotation databases
EnsemblMetazoai | FBtr0085550; FBpp0084916; FBgn0039734 [Q9VAC5-2] FBtr0085551; FBpp0084917; FBgn0039734 [Q9VAC5-1] |
GeneIDi | 43558 |
KEGGi | dme:Dmel_CG7908 |
UCSCi | CG7908-RA, d. melanogaster [Q9VAC5-1] |
Organism-specific databases
CTDi | 43558 |
FlyBasei | FBgn0039734, Tace |
Phylogenomic databases
eggNOGi | KOG3658, Eukaryota |
GeneTreei | ENSGT00940000155443 |
HOGENOMi | CLU_004602_2_0_1 |
InParanoidi | Q9VAC5 |
OMAi | FYHGRVF |
PhylomeDBi | Q9VAC5 |
Enzyme and pathway databases
SignaLinki | Q9VAC5 |
Miscellaneous databases
BioGRID-ORCSi | 43558, 0 hits in 1 CRISPR screen |
GenomeRNAii | 43558 |
PROi | PR:Q9VAC5 |
Gene expression databases
Bgeei | FBgn0039734, Expressed in central nervous system and 21 other tissues |
ExpressionAtlasi | Q9VAC5, baseline and differential |
Genevisiblei | Q9VAC5, DM |
Family and domain databases
CDDi | cd14246, ADAM17_MPD, 1 hit cd04270, ZnMc_TACE_like, 1 hit |
Gene3Di | 3.40.390.10, 1 hit 4.10.70.10, 1 hit |
InterProi | View protein in InterPro IPR034025, ADAM10_ADAM17 IPR032029, ADAM17_MPD IPR001762, Disintegrin_dom IPR036436, Disintegrin_dom_sf IPR024079, MetalloPept_cat_dom_sf IPR001590, Peptidase_M12B IPR002870, Peptidase_M12B_N |
Pfami | View protein in Pfam PF16698, ADAM17_MPD, 1 hit PF00200, Disintegrin, 1 hit PF01562, Pep_M12B_propep, 1 hit |
SMARTi | View protein in SMART SM00050, DISIN, 1 hit |
SUPFAMi | SSF57552, SSF57552, 1 hit |
PROSITEi | View protein in PROSITE PS50215, ADAM_MEPRO, 1 hit PS50214, DISINTEGRIN_2, 1 hit PS00142, ZINC_PROTEASE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ADA17_DROME | |
Accessioni | Q9VAC5Primary (citable) accession number: Q9VAC5 Secondary accession number(s): Q868A7, Q8IMJ9, Q95RZ4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 20, 2001 |
Last sequence update: | October 10, 2003 | |
Last modified: | April 7, 2021 | |
This is version 160 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase