UniProtKB - Q9VA69 (Q9VA69_DROME)
Protein
Procollagen-proline 4-dioxygenase
Gene
PH4alphaEFB
Organism
Drosophila melanogaster (Fruit fly)
Status
Functioni
Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.ARBA annotation
Catalytic activityi
- 2-oxoglutarate + L-prolyl-[procollagen] + O2 = CO2 + succinate + trans-4-hydroxy-L-prolyl-[procollagen]ARBA annotationEC:1.14.11.2ARBA annotation
Cofactori
L-ascorbateARBA annotation
GO - Molecular functioni
- iron ion binding Source: InterPro
- L-ascorbic acid binding Source: InterPro
- oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen Source: InterPro
- procollagen-proline 4-dioxygenase activity Source: UniProtKB-EC
GO - Biological processi
- peptidyl-proline hydroxylation to 4-hydroxy-L-proline Source: GO_Central
Keywordsi
Molecular function | DioxygenaseARBA annotation, Oxidoreductase |
Ligand | IronARBA annotation, Metal-bindingARBA annotation |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:PH4alphaEFBImported Synonyms:BcDNA:SD05564Imported, CG12088Imported, CG1473Imported, Dmel\CG31022Imported, PH4-aEFBImported, PH4-alpha-EFBImported, PH4alpha-EFBImported ORF Names:CG31022Imported, Dmel_CG31022Imported |
Organismi | Drosophila melanogaster (Fruit fly)Imported |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Holometabola › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0039776, PH4alphaEFB |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum lumen ARBA annotation
Endoplasmic reticulum
- endoplasmic reticulum Source: GO_Central
- endoplasmic reticulum lumen Source: UniProtKB-SubCell
Other locations
- endomembrane system Source: FlyBase
Keywords - Cellular componenti
Endoplasmic reticulumARBA annotationPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 23 | Sequence analysisAdd BLAST | 23 | |
ChainiPRO_5015100063 | 24 – 550 | Procollagen-proline 4-dioxygenaseSequence analysisAdd BLAST | 527 |
Keywords - PTMi
GlycoproteinARBA annotationExpressioni
Gene expression databases
Bgeei | FBgn0039776, Expressed in spermathecum and 46 other tissues |
Interactioni
Protein-protein interaction databases
IntActi | Q9VA69, 2 interactors |
STRINGi | 7227.FBpp0085012 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 422 – 530 | Fe2OG dioxygenaseInterPro annotationAdd BLAST | 109 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 50 – 77 | Sequence analysisAdd BLAST | 28 |
Sequence similaritiesi
Belongs to the P4HA family.ARBA annotation
Keywords - Domaini
Coiled coilSequence analysis, SignalSequence analysisPhylogenomic databases
eggNOGi | KOG1591, Eukaryota |
GeneTreei | ENSGT00940000163795 |
HOGENOMi | CLU_024155_1_1_1 |
OMAi | MYDSEID |
OrthoDBi | 391515at2759 |
Family and domain databases
Gene3Di | 1.25.40.10, 1 hit |
InterProi | View protein in InterPro IPR005123, Oxoglu/Fe-dep_dioxygenase IPR006620, Pro_4_hyd_alph IPR013547, Pro_4_hyd_alph_N IPR011990, TPR-like_helical_dom_sf |
Pfami | View protein in Pfam PF13640, 2OG-FeII_Oxy_3, 1 hit PF08336, P4Ha_N, 1 hit |
SMARTi | View protein in SMART SM00702, P4Hc, 1 hit |
SUPFAMi | SSF48452, SSF48452, 1 hit |
PROSITEi | View protein in PROSITE PS51471, FE2OG_OXY, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9VA69-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAADWRLMLL LGILLLVGGP ANGEVYTALA EMEELLETES VLITNLEGYI
60 70 80 90 100
RVQEDKLNFL KNKMDEYQRE HSDASHDITA YVSNPINAYL LTKRLTTDWR
110 120 130 140 150
QVENLMEHDV GTDFLQNITQ YRSLLKFPSD EDLNGAAVAL LRLQDTYQLD
160 170 180 190 200
TSSVARGKLN GIQYSTEMSS DDCFELGRQS YVNHDYYHTV LWMNEAMARM
210 220 230 240 250
LEEPTNHTQS FTKADILEYL AFSTYKEGNI ESALTMTNEL LQLLPHHERA
260 270 280 290 300
NGNKRFYEKE IAQQLQLRKM KGDDGTDEMP KSDLPVAKSD PAIFDMTERR
310 320 330 340 350
AYEMLCRGEL KPSPSDLRSL RCRYVTNRVP FLRLGPLKLE EVHADPYIVI
360 370 380 390 400
YHDAMYDSEI DLIKRMARPR FRRATVQNSV TGALETANYR ISKSAWLKTQ
410 420 430 440 450
EDRVIETVVQ RTADMTGLDM DSAEELQVVN YGIGGHYEPH FDFARKEEQR
460 470 480 490 500
AFEGLNLGNR IATVLFYMSD VEQGGATVFT SLHTALFPKK GTAAFWMNLH
510 520 530 540 550
RDGQGDVRTR HAACPVLTGT KWVSNKWIHE RGQEFRRPCD LEEDHGEFAI
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE014297 Genomic DNA Translation: AAF57053.2 AY052109 mRNA Translation: AAK93533.1 AF495536 mRNA Translation: AAM18058.1 |
RefSeqi | NP_733371.1, NM_170492.2 |
Genome annotation databases
EnsemblMetazoai | FBtr0085650; FBpp0085012; FBgn0039776 |
GeneIDi | 43620 |
KEGGi | dme:Dmel_CG31022 |
UCSCi | CG31022-RA, d. melanogaster |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE014297 Genomic DNA Translation: AAF57053.2 AY052109 mRNA Translation: AAK93533.1 AF495536 mRNA Translation: AAM18058.1 |
RefSeqi | NP_733371.1, NM_170492.2 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
IntActi | Q9VA69, 2 interactors |
STRINGi | 7227.FBpp0085012 |
Genome annotation databases
EnsemblMetazoai | FBtr0085650; FBpp0085012; FBgn0039776 |
GeneIDi | 43620 |
KEGGi | dme:Dmel_CG31022 |
UCSCi | CG31022-RA, d. melanogaster |
Organism-specific databases
CTDi | 43620 |
FlyBasei | FBgn0039776, PH4alphaEFB |
Phylogenomic databases
eggNOGi | KOG1591, Eukaryota |
GeneTreei | ENSGT00940000163795 |
HOGENOMi | CLU_024155_1_1_1 |
OMAi | MYDSEID |
OrthoDBi | 391515at2759 |
Miscellaneous databases
BioGRID-ORCSi | 43620, 0 hits in 3 CRISPR screens |
GenomeRNAii | 43620 |
Gene expression databases
Bgeei | FBgn0039776, Expressed in spermathecum and 46 other tissues |
Family and domain databases
Gene3Di | 1.25.40.10, 1 hit |
InterProi | View protein in InterPro IPR005123, Oxoglu/Fe-dep_dioxygenase IPR006620, Pro_4_hyd_alph IPR013547, Pro_4_hyd_alph_N IPR011990, TPR-like_helical_dom_sf |
Pfami | View protein in Pfam PF13640, 2OG-FeII_Oxy_3, 1 hit PF08336, P4Ha_N, 1 hit |
SMARTi | View protein in SMART SM00702, P4Hc, 1 hit |
SUPFAMi | SSF48452, SSF48452, 1 hit |
PROSITEi | View protein in PROSITE PS51471, FE2OG_OXY, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | Q9VA69_DROME | |
Accessioni | Q9VA69Primary (citable) accession number: Q9VA69 Secondary accession number(s): Q960D8 | |
Entry historyi | Integrated into UniProtKB/TrEMBL: | May 1, 2000 |
Last sequence update: | October 1, 2002 | |
Last modified: | February 10, 2021 | |
This is version 174 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Unreviewed (UniProtKB/TrEMBL) |