UniProtKB - Q9VA63 (Q9VA63_DROME)
Protein
Procollagen-proline 4-dioxygenase
Gene
PH4alphaSG1
Organism
Drosophila melanogaster (Fruit fly)
Status
Functioni
Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.ARBA annotation
Catalytic activityi
- 2-oxoglutarate + L-prolyl-[collagen] + O2 = CO2 + succinate + trans-4-hydroxy-L-prolyl-[collagen]ARBA annotationEC:1.14.11.2ARBA annotation
Cofactori
L-ascorbateARBA annotation
GO - Molecular functioni
- iron ion binding Source: InterPro
- L-ascorbic acid binding Source: InterPro
- oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen Source: InterPro
- procollagen-proline 4-dioxygenase activity Source: UniProtKB-EC
GO - Biological processi
- peptidyl-proline hydroxylation to 4-hydroxy-L-proline Source: GO_Central
- salivary gland morphogenesis Source: FlyBase
Keywordsi
Molecular function | DioxygenaseARBA annotation, Oxidoreductase |
Ligand | IronARBA annotation, Metal-bindingARBA annotation |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:PH4alphaSG1Imported Synonyms:alphaSG1Imported, CG15542Imported, Dmel\CG31014Imported, PH4-alpha-SG1Imported, Ph4alphaSG1Imported, PHalphaSG1Imported, SG1Imported ORF Names:CG31014Imported, Dmel_CG31014Imported |
Organismi | Drosophila melanogaster (Fruit fly)Imported |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Holometabola › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0051014, PH4alphaSG1 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum lumen ARBA annotation
Endoplasmic reticulum
- endoplasmic reticulum Source: FlyBase
- endoplasmic reticulum lumen Source: UniProtKB-SubCell
Keywords - Cellular componenti
Endoplasmic reticulumARBA annotationPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 18 | Sequence analysisAdd BLAST | 18 | |
ChainiPRO_5015100060 | 19 – 540 | Procollagen-proline 4-dioxygenaseSequence analysisAdd BLAST | 522 |
Keywords - PTMi
GlycoproteinARBA annotationExpressioni
Gene expression databases
Bgeei | FBgn0051014, Expressed in saliva-secreting gland and 17 other tissues |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 410 – 516 | Fe2OG dioxygenaseInterPro annotationAdd BLAST | 107 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 41 – 75 | Sequence analysisAdd BLAST | 35 |
Sequence similaritiesi
Belongs to the P4HA family.ARBA annotation
Keywords - Domaini
Coiled coilSequence analysis, SignalSequence analysisPhylogenomic databases
eggNOGi | KOG1591, Eukaryota |
GeneTreei | ENSGT00940000163795 |
HOGENOMi | CLU_024155_1_1_1 |
OMAi | QGSKWIC |
OrthoDBi | 391515at2759 |
Family and domain databases
Gene3Di | 1.25.40.10, 1 hit |
InterProi | View protein in InterPro IPR005123, Oxoglu/Fe-dep_dioxygenase IPR006620, Pro_4_hyd_alph IPR013547, Pro_4_hyd_alph_N IPR011990, TPR-like_helical_dom_sf |
Pfami | View protein in Pfam PF13640, 2OG-FeII_Oxy_3, 1 hit PF08336, P4Ha_N, 1 hit |
SMARTi | View protein in SMART SM00702, P4Hc, 1 hit |
PROSITEi | View protein in PROSITE PS51471, FE2OG_OXY, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9VA63-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MFRLSIILSL LCVFPVNGDY YSAISELERL LDVEAFIVEK FDEYLERAQQ
60 70 80 90 100
EQENLKRFLD QIDEQQNDRG DLEEYFGNPI NAFITIKRLV HDWKFNVFDP
110 120 130 140 150
VFDSSNFETY KNNLSDSLEK INFKGPTQED LHGATRALLR LQNMYQLNTD
160 170 180 190 200
HLASGVLHPG EKNALGTSLS ASDCFELGKN LCEIKEYSYG SEWLLEARKR
210 220 230 240 250
LHGKPLGFIS PNVSDVEILE HLSSAFNGLG NLKLAYKLNN EILDKKPDHE
260 270 280 290 300
EALKNKILYE GQLARERSFA PRKQVELPQI AEKEQKESYK LYTQVCRGEL
310 320 330 340 350
HQSPREQRNL RCWLYHQGVP YYRLSPFKIE QLNVDPYVAY VHEVLWDSEI
360 370 380 390 400
DTIMEHGKGN MERSKVGQSE NSTTSEVRIS RNTWLWYDAN PWLSKIKQRL
410 420 430 440 450
EDVTGLSTES AEPLQLVNYG IGGQYEPHFD FVEDDGQSVF SWKGNRLLTA
460 470 480 490 500
LFYLNDVALG GATAFPFLRL AVPPVKGSLL IWYNLHSSTH KDFRTKHAGC
510 520 530 540
PVLQGSKWIC NEWFHVGAQE FRRPCGLSSD EGKFLHLKDI
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE014297 Genomic DNA Translation: AAF57059.2 BT023117 mRNA Translation: AAY55533.1 |
RefSeqi | NP_733376.1, NM_170497.4 |
Genome annotation databases
EnsemblMetazoai | FBtr0085686; FBpp0085048; FBgn0051014 |
GeneIDi | 326112 |
KEGGi | dme:Dmel_CG31014 |
UCSCi | CG31014-RA, d. melanogaster |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE014297 Genomic DNA Translation: AAF57059.2 BT023117 mRNA Translation: AAY55533.1 |
RefSeqi | NP_733376.1, NM_170497.4 |
3D structure databases
SMRi | Q9VA63 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 7227.FBpp0085048 |
Protocols and materials databases
DNASUi | 326112 |
Genome annotation databases
EnsemblMetazoai | FBtr0085686; FBpp0085048; FBgn0051014 |
GeneIDi | 326112 |
KEGGi | dme:Dmel_CG31014 |
UCSCi | CG31014-RA, d. melanogaster |
Organism-specific databases
CTDi | 326112 |
FlyBasei | FBgn0051014, PH4alphaSG1 |
Phylogenomic databases
eggNOGi | KOG1591, Eukaryota |
GeneTreei | ENSGT00940000163795 |
HOGENOMi | CLU_024155_1_1_1 |
OMAi | QGSKWIC |
OrthoDBi | 391515at2759 |
Miscellaneous databases
BioGRID-ORCSi | 326112, 0 hits in 1 CRISPR screen |
GenomeRNAii | 326112 |
Gene expression databases
Bgeei | FBgn0051014, Expressed in saliva-secreting gland and 17 other tissues |
Family and domain databases
Gene3Di | 1.25.40.10, 1 hit |
InterProi | View protein in InterPro IPR005123, Oxoglu/Fe-dep_dioxygenase IPR006620, Pro_4_hyd_alph IPR013547, Pro_4_hyd_alph_N IPR011990, TPR-like_helical_dom_sf |
Pfami | View protein in Pfam PF13640, 2OG-FeII_Oxy_3, 1 hit PF08336, P4Ha_N, 1 hit |
SMARTi | View protein in SMART SM00702, P4Hc, 1 hit |
PROSITEi | View protein in PROSITE PS51471, FE2OG_OXY, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | Q9VA63_DROME | |
Accessioni | Q9VA63Primary (citable) accession number: Q9VA63 | |
Entry historyi | Integrated into UniProtKB/TrEMBL: | May 1, 2000 |
Last sequence update: | October 1, 2002 | |
Last modified: | April 7, 2021 | |
This is version 172 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Unreviewed (UniProtKB/TrEMBL) |