UniProtKB - Q9V535 (RBM8A_DROME)
Protein
RNA-binding protein 8A
Gene
tsu
Organism
Drosophila melanogaster (Fruit fly)
Status
Functioni
Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs (PubMed:14973490, PubMed:24967911). Involved in exon definition of genes containing long introns, including the rolled/MAPK gene (PubMed:20946982, PubMed:20946983). The mago-tsu heterodimer interacts with the EJC key regulator Pym leading to EJC disassembly in the cytoplasm (PubMed:24967911). Has a role in oskar mRNA localization to the posterior pole of the developing oocyte (PubMed:11691839).5 Publications
Miscellaneous
'Tsunagi' means 'connection' or 'link' in Japanese.1 Publication
GO - Molecular functioni
- mRNA binding Source: FlyBase
GO - Biological processi
- establishment of pole plasm mRNA localization Source: FlyBase
- germarium-derived oocyte fate determination Source: FlyBase
- microtubule cytoskeleton organization Source: FlyBase
- mRNA splicing, via spliceosome Source: FlyBase
- mRNA transport Source: UniProtKB-KW
- negative regulation of transposition, DNA-mediated Source: FlyBase
- nuclear-transcribed mRNA catabolic process, nonsense-mediated decay Source: GO_Central
- oocyte anterior/posterior axis specification Source: FlyBase
- oocyte dorsal/ventral axis specification Source: FlyBase
- oocyte nucleus localization involved in oocyte dorsal/ventral axis specification Source: FlyBase
- pole plasm oskar mRNA localization Source: FlyBase
- regulation of alternative mRNA splicing, via spliceosome Source: GO_Central
- regulation of pole plasm oskar mRNA localization Source: FlyBase
- RNA splicing Source: FlyBase
Keywordsi
Molecular function | Developmental protein, RNA-binding |
Biological process | mRNA processing, mRNA splicing, mRNA transport, Transport |
Enzyme and pathway databases
Reactomei | R-DME-159236, Transport of Mature mRNA derived from an Intron-Containing Transcript R-DME-72163, mRNA Splicing - Major Pathway R-DME-72187, mRNA 3'-end processing R-DME-73856, RNA Polymerase II Transcription Termination R-DME-975957, Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) |
Names & Taxonomyi
Protein namesi | Recommended name: RNA-binding protein 8AAlternative name(s): Protein tsunagi |
Gene namesi | ORF Names:CG8781Imported |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Holometabola › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0033378, tsu |
Subcellular locationi
Nucleus
- catalytic step 2 spliceosome Source: FlyBase
- exon-exon junction complex Source: FlyBase
- nucleus Source: FlyBase
Other locations
- cytoplasm Source: FlyBase
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Disruption phenotypei
Developing photoreceptor cell clusters express reduced levels of rolled/MAPK and fail to differentiate normally.2 Publications
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000081765 | 1 – 165 | RNA-binding protein 8AAdd BLAST | 165 |
Proteomic databases
PaxDbi | Q9V535 |
PRIDEi | Q9V535 |
Expressioni
Developmental stagei
Expressed throughout development. Localizes to the posterior pole of the oocyte during stages 1-9 of oogenesis.1 Publication
Gene expression databases
Bgeei | FBgn0033378, Expressed in embryo and 27 other tissues |
ExpressionAtlasi | Q9V535, baseline and differential |
Genevisiblei | Q9V535, DM |
Interactioni
Subunit structurei
Heterodimer (via RRM domain) with mago (PubMed:11691839, PubMed:12730685, PubMed:12704080). Part of the mRNA splicing-dependent exon junction complex (EJC) complex; the core complex contains btz/CASC3, eIF4AIII, mago and tsu/RBM8A (PubMed:14973490).
Interacts with Pym (via N-terminus); the interaction is direct (PubMed:24967911, PubMed:14968132).
Interacts with eIF4AIII (PubMed:14973490).
6 PublicationsBinary interactionsi
Q9V535
With | #Exp. | IntAct |
---|---|---|
mago [P49028] | 6 | EBI-172458,EBI-159609 |
Protein-protein interaction databases
BioGRIDi | 61762, 13 interactors |
ComplexPortali | CPX-3100, mago-y14 exon-exon junction subcomplex CPX-3147, PYM-mago-Y14 complex CPX-3171, cdm-mago-Y14 complex |
DIPi | DIP-24075N |
IntActi | Q9V535, 2 interactors |
STRINGi | 7227.FBpp0087648 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q9V535 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9V535 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 73 – 151 | RRMPROSITE-ProRule annotationAdd BLAST | 79 |
Domaini
The RNA recognition motif (RRM) is involved in the interaction with mago. The RNA-binding activity of such domains is therefore uncertain.1 Publication
Sequence similaritiesi
Belongs to the RBM8A family.Curated
Phylogenomic databases
eggNOGi | KOG0130, Eukaryota |
GeneTreei | ENSGT00730000111185 |
HOGENOMi | CLU_012062_18_3_1 |
InParanoidi | Q9V535 |
OMAi | RDDVGHY |
OrthoDBi | 1444995at2759 |
PhylomeDBi | Q9V535 |
Family and domain databases
CDDi | cd12324, RRM_RBM8, 1 hit |
Gene3Di | 3.30.70.330, 1 hit |
InterProi | View protein in InterPro IPR012677, Nucleotide-bd_a/b_plait_sf IPR035979, RBD_domain_sf IPR008111, RNA-bd_8 IPR000504, RRM_dom IPR033744, RRM_RBM8 |
Pfami | View protein in Pfam PF00076, RRM_1, 1 hit |
PRINTSi | PR01738, RNABINDINGM8 |
SMARTi | View protein in SMART SM00360, RRM, 1 hit |
SUPFAMi | SSF54928, SSF54928, 1 hit |
PROSITEi | View protein in PROSITE PS50102, RRM, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9V535-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MADVLDIDNA EEFEVDEDGD QGIVRLKEKA KHRKGRGFGS DSNTREAIHS
60 70 80 90 100
YERVRNEDDD ELEPGPQRSV EGWILFVTSI HEEAQEDEIQ EKFCDYGEIK
110 120 130 140 150
NIHLNLDRRT GFSKGYALVE YETHKQALAA KEALNGAEIM GQTIQVDWCF
160
VKGPKRVKKS EKRRR
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 86 | E → G in AAL29185 (PubMed:11691839).Curated | 1 | |
Sequence conflicti | 101 | N → S in AAL48627 (PubMed:12537569).Curated | 1 | |
Sequence conflicti | 135 | N → R in AAL29185 (PubMed:11691839).Curated | 1 | |
Sequence conflicti | 159 | K → R in AAL48627 (PubMed:12537569).Curated | 1 | |
Sequence conflicti | 162 | K → M in AAL29185 (PubMed:11691839).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF173550 mRNA Translation: AAL29185.1 AE013599 Genomic DNA Translation: AAF58987.1 AY071005 mRNA Translation: AAL48627.1 |
RefSeqi | NP_610454.2, NM_136610.4 |
Genome annotation databases
EnsemblMetazoai | FBtr0088567; FBpp0087648; FBgn0033378 |
GeneIDi | 35924 |
KEGGi | dme:Dmel_CG8781 |
UCSCi | CG8781-RA, d. melanogaster |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF173550 mRNA Translation: AAL29185.1 AE013599 Genomic DNA Translation: AAF58987.1 AY071005 mRNA Translation: AAL48627.1 |
RefSeqi | NP_610454.2, NM_136610.4 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1HL6 | X-ray | 2.50 | A/C | 1-165 | [»] | |
1OO0 | X-ray | 1.85 | B | 47-156 | [»] | |
1RK8 | X-ray | 1.90 | A | 1-165 | [»] | |
2X1G | X-ray | 3.35 | A/C | 1-165 | [»] | |
SMRi | Q9V535 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 61762, 13 interactors |
ComplexPortali | CPX-3100, mago-y14 exon-exon junction subcomplex CPX-3147, PYM-mago-Y14 complex CPX-3171, cdm-mago-Y14 complex |
DIPi | DIP-24075N |
IntActi | Q9V535, 2 interactors |
STRINGi | 7227.FBpp0087648 |
Proteomic databases
PaxDbi | Q9V535 |
PRIDEi | Q9V535 |
Protocols and materials databases
ABCDi | Q9V535, 4 sequenced antibodies |
Genome annotation databases
EnsemblMetazoai | FBtr0088567; FBpp0087648; FBgn0033378 |
GeneIDi | 35924 |
KEGGi | dme:Dmel_CG8781 |
UCSCi | CG8781-RA, d. melanogaster |
Organism-specific databases
CTDi | 110124 |
FlyBasei | FBgn0033378, tsu |
Phylogenomic databases
eggNOGi | KOG0130, Eukaryota |
GeneTreei | ENSGT00730000111185 |
HOGENOMi | CLU_012062_18_3_1 |
InParanoidi | Q9V535 |
OMAi | RDDVGHY |
OrthoDBi | 1444995at2759 |
PhylomeDBi | Q9V535 |
Enzyme and pathway databases
Reactomei | R-DME-159236, Transport of Mature mRNA derived from an Intron-Containing Transcript R-DME-72163, mRNA Splicing - Major Pathway R-DME-72187, mRNA 3'-end processing R-DME-73856, RNA Polymerase II Transcription Termination R-DME-975957, Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) |
Miscellaneous databases
BioGRID-ORCSi | 35924, 1 hit in 1 CRISPR screen |
EvolutionaryTracei | Q9V535 |
GenomeRNAii | 35924 |
PROi | PR:Q9V535 |
Gene expression databases
Bgeei | FBgn0033378, Expressed in embryo and 27 other tissues |
ExpressionAtlasi | Q9V535, baseline and differential |
Genevisiblei | Q9V535, DM |
Family and domain databases
CDDi | cd12324, RRM_RBM8, 1 hit |
Gene3Di | 3.30.70.330, 1 hit |
InterProi | View protein in InterPro IPR012677, Nucleotide-bd_a/b_plait_sf IPR035979, RBD_domain_sf IPR008111, RNA-bd_8 IPR000504, RRM_dom IPR033744, RRM_RBM8 |
Pfami | View protein in Pfam PF00076, RRM_1, 1 hit |
PRINTSi | PR01738, RNABINDINGM8 |
SMARTi | View protein in SMART SM00360, RRM, 1 hit |
SUPFAMi | SSF54928, SSF54928, 1 hit |
PROSITEi | View protein in PROSITE PS50102, RRM, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RBM8A_DROME | |
Accessioni | Q9V535Primary (citable) accession number: Q9V535 Secondary accession number(s): Q8SZA6, Q95X08 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 25, 2004 |
Last sequence update: | May 1, 2000 | |
Last modified: | December 2, 2020 | |
This is version 153 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families