UniProtKB - Q9V0U0 (GATE_PYRAB)
Protein
Glutamyl-tRNA(Gln) amidotransferase subunit E
Gene
gatE
Organism
Pyrococcus abyssi (strain GE5 / Orsay)
Status
Functioni
Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln). The GatDE system is specific for glutamate and does not act on aspartate.UniRule annotation
Catalytic activityi
- ATP + H2O + L-glutamine + L-glutamyl-tRNAGln = ADP + H+ + L-glutamate + L-glutaminyl-tRNAGln + phosphateUniRule annotation
GO - Molecular functioni
- aminoacyl-tRNA ligase activity Source: InterPro
- ATP binding Source: UniProtKB-KW
- glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity Source: UniProtKB-UniRule
GO - Biological processi
- translation Source: UniProtKB-UniRule
Keywordsi
Molecular function | Ligase |
Biological process | Protein biosynthesis |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Glutamyl-tRNA(Gln) amidotransferase subunit EUniRule annotation (EC:6.3.5.-UniRule annotation)Short name: Glu-ADT subunit EUniRule annotation |
Gene namesi | Name:gatEUniRule annotation Ordered Locus Names:PYRAB06990 ORF Names:PAB1902 |
Organismi | Pyrococcus abyssi (strain GE5 / Orsay) |
Taxonomic identifieri | 272844 [NCBI] |
Taxonomic lineagei | Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000140076 | 1 – 633 | Glutamyl-tRNA(Gln) amidotransferase subunit EAdd BLAST | 633 |
Interactioni
Subunit structurei
Heterodimer of GatD and GatE.
UniRule annotationBinary interactionsi
Q9V0U0
With | #Exp. | IntAct |
---|---|---|
gatD [Q9V0T9] | 3 | EBI-9023893,EBI-9023883 |
Protein-protein interaction databases
DIPi | DIP-48454N |
IntActi | Q9V0U0, 1 interactor |
STRINGi | 272844.PAB1902 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q9V0U0 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9V0U0 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | arCOG01719, Archaea |
HOGENOMi | CLU_030702_0_0_2 |
OMAi | SGFQRTM |
Family and domain databases
DisProti | DP01919 |
Gene3Di | 1.10.10.410, 1 hit 1.10.150.380, 1 hit 3.30.1360.30, 1 hit |
HAMAPi | MF_00588, GatE, 1 hit |
InterProi | View protein in InterPro IPR017959, Asn/Gln-tRNA_amidoTrfase_suB/E IPR006075, Asn/Gln-tRNA_Trfase_suB/E_cat IPR018027, Asn/Gln_amidotransferase IPR003789, Asn/Gln_tRNA_amidoTrase-B-like IPR004115, GAD-like_sf IPR029351, GAD_dom IPR042114, GatB_C_1 IPR023168, GatB_Yqey_C_2 IPR004414, GatE IPR017958, Gln-tRNA_amidoTrfase_suB_CS IPR014746, Gln_synth/guanido_kin_cat_dom |
PANTHERi | PTHR11659, PTHR11659, 1 hit PTHR11659:SF2, PTHR11659:SF2, 1 hit |
Pfami | View protein in Pfam PF02938, GAD, 1 hit PF02934, GatB_N, 1 hit PF02637, GatB_Yqey, 1 hit |
SMARTi | View protein in SMART SM00845, GatB_Yqey, 1 hit |
SUPFAMi | SSF55261, SSF55261, 1 hit SSF55931, SSF55931, 1 hit SSF89095, SSF89095, 1 hit |
TIGRFAMsi | TIGR00134, gatE_arch, 1 hit |
PROSITEi | View protein in PROSITE PS01234, GATB, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9V0U0-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MMVMTDKFNY EELGLKVGLE IHRQLDTKKL FSPVPSELSD KVEFTFQRRL
60 70 80 90 100
RPTMSELGEI DPAALEEFKK GRVYVYEGNY ELTDLVYMDE EPPRGPDREA
110 120 130 140 150
LEVALQIAYL LNAKPVDEVY YMRKIVIDGS NVSGFQRTAI IATDGKVETP
160 170 180 190 200
WGAVGIPTIC LEEDAARIIE RKDKEVIYRL DRLGIPLIEI STTPDIHHPE
210 220 230 240 250
QAKVVAKFIG DALRATKKVK RGLGTIRQDL NVSIKGGARI EIKGVQELDM
260 270 280 290 300
IPIIIEREVE RQLNLLKIRD ELRKRGVKPK DIKEEFYDVT DIFENTKSKI
310 320 330 340 350
IARVIKKGGK VLAIKLPKFR GLIGREIQPG RRLGTEFADR AKKYVPGIFH
360 370 380 390 400
IDELPNYGIS QEEVNKVIER LNLSEEDAFV LVAAEEEKAK NALREVIKRA
410 420 430 440 450
REAIEGVPEE TRRALPDGNT EYMRPLPGKA RMYPETDIPP LRIPDDLKKK
460 470 480 490 500
IKENLPELPQ AKVERYVKEY KLDRSLAQTL VDDERDELFE ELVSMGVKPS
510 520 530 540 550
LAASILVVVL KGLRKEVPIE NVTDEHIREA FQLYLEGKIA KEAFEEIFKE
560 570 580 590 600
LARNPSKSAR EVAEEKGLTL LSEEEVTRII EEVIQQNIEV VKAKGMGAMG
610 620 630
LIMGRVMAKV RGKADGKLVS QIVRRKLQEI SGG
Sequence cautioni
The sequence CCE70090 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ248285 Genomic DNA Translation: CAB49613.1 HE613800 Genomic DNA Translation: CCE70090.1 Different initiation. |
PIRi | D75112 |
Genome annotation databases
EnsemblBacteriai | CAB49613; CAB49613; PAB1902 |
KEGGi | pab:PAB1902 |
PATRICi | fig|272844.11.peg.734 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ248285 Genomic DNA Translation: CAB49613.1 HE613800 Genomic DNA Translation: CCE70090.1 Different initiation. |
PIRi | D75112 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1ZQ1 | X-ray | 3.00 | C/D | 1-633 | [»] | |
SMRi | Q9V0U0 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
DIPi | DIP-48454N |
IntActi | Q9V0U0, 1 interactor |
STRINGi | 272844.PAB1902 |
Genome annotation databases
EnsemblBacteriai | CAB49613; CAB49613; PAB1902 |
KEGGi | pab:PAB1902 |
PATRICi | fig|272844.11.peg.734 |
Phylogenomic databases
eggNOGi | arCOG01719, Archaea |
HOGENOMi | CLU_030702_0_0_2 |
OMAi | SGFQRTM |
Miscellaneous databases
EvolutionaryTracei | Q9V0U0 |
Family and domain databases
DisProti | DP01919 |
Gene3Di | 1.10.10.410, 1 hit 1.10.150.380, 1 hit 3.30.1360.30, 1 hit |
HAMAPi | MF_00588, GatE, 1 hit |
InterProi | View protein in InterPro IPR017959, Asn/Gln-tRNA_amidoTrfase_suB/E IPR006075, Asn/Gln-tRNA_Trfase_suB/E_cat IPR018027, Asn/Gln_amidotransferase IPR003789, Asn/Gln_tRNA_amidoTrase-B-like IPR004115, GAD-like_sf IPR029351, GAD_dom IPR042114, GatB_C_1 IPR023168, GatB_Yqey_C_2 IPR004414, GatE IPR017958, Gln-tRNA_amidoTrfase_suB_CS IPR014746, Gln_synth/guanido_kin_cat_dom |
PANTHERi | PTHR11659, PTHR11659, 1 hit PTHR11659:SF2, PTHR11659:SF2, 1 hit |
Pfami | View protein in Pfam PF02938, GAD, 1 hit PF02934, GatB_N, 1 hit PF02637, GatB_Yqey, 1 hit |
SMARTi | View protein in SMART SM00845, GatB_Yqey, 1 hit |
SUPFAMi | SSF55261, SSF55261, 1 hit SSF55931, SSF55931, 1 hit SSF89095, SSF89095, 1 hit |
TIGRFAMsi | TIGR00134, gatE_arch, 1 hit |
PROSITEi | View protein in PROSITE PS01234, GATB, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GATE_PYRAB | |
Accessioni | Q9V0U0Primary (citable) accession number: Q9V0U0 Secondary accession number(s): G8ZJG3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 25, 2002 |
Last sequence update: | May 1, 2000 | |
Last modified: | December 2, 2020 | |
This is version 126 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structureDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families