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Protein

5-formaminoimidazole-4-carboxamide-1-(beta)-D-ribofuranosyl 5'-monophosphate synthetase

Gene

purP

Organism
Pyrococcus abyssi (strain GE5 / Orsay)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the ATP- and formate-dependent formylation of 5-aminoimidazole-4-carboxamide-1-beta-d-ribofuranosyl 5'-monophosphate (AICAR) to 5-formaminoimidazole-4-carboxamide-1-beta-d-ribofuranosyl 5'-monophosphate (FAICAR) in the absence of folates.UniRule annotation

Catalytic activityi

ATP + formate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = ADP + phosphate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide.UniRule annotation

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 1 Mg2+ or Mn2+ ion per subunit.By similarity

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (formate route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. 5-formaminoimidazole-4-carboxamide-1-(beta)-D-ribofuranosyl 5'-monophosphate synthetase (purP)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (formate route), the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei14SubstrateUniRule annotation1
Binding sitei74SubstrateUniRule annotation1
Binding sitei207ATPUniRule annotation1
Binding sitei235SubstrateUniRule annotation1
Metal bindingi273Magnesium or manganeseUniRule annotation1
Metal bindingi286Magnesium or manganeseUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi125 – 185ATPUniRule annotationAdd BLAST61

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processPurine biosynthesis
LigandATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayi
UPA00074;UER00134

Names & Taxonomyi

Protein namesi
Recommended name:
5-formaminoimidazole-4-carboxamide-1-(beta)-D-ribofuranosyl 5'-monophosphate synthetaseUniRule annotation (EC:6.3.4.23UniRule annotation)
Alternative name(s):
5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranosyl 5'-monophosphate--formate ligaseUniRule annotation
Gene namesi
Name:purPUniRule annotation
Ordered Locus Names:PYRAB08010
ORF Names:PAB0547
OrganismiPyrococcus abyssi (strain GE5 / Orsay)
Taxonomic identifieri272844 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000810 Componenti: Chromosome
  • UP000009139 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003486351 – 3375-formaminoimidazole-4-carboxamide-1-(beta)-D-ribofuranosyl 5'-monophosphate synthetaseAdd BLAST337

Interactioni

Protein-protein interaction databases

STRINGi272844.PAB0547

Structurei

3D structure databases

ProteinModelPortaliQ9V0I8
SMRiQ9V0I8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini81 – 328ATP-graspUniRule annotationAdd BLAST248

Sequence similaritiesi

Belongs to the phosphohexose mutase family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG04346 Archaea
COG1759 LUCA
HOGENOMiHOG000228474
KOiK06863
OMAiCLEGVFT
OrthoDBiPOG093Z04BP

Family and domain databases

Gene3Di3.30.1490.20, 1 hit
HAMAPiMF_01163 IMP_biosynth_PurP, 1 hit
InterProiView protein in InterPro
IPR011761 ATP-grasp
IPR013815 ATP_grasp_subdomain_1
IPR023656 IMP_biosynth_PurP
IPR009720 IMP_biosynth_PurP_C
IPR010672 IMP_biosynth_PurP_N
IPR016185 PreATP-grasp_dom_sf
PANTHERiPTHR38147 PTHR38147, 1 hit
PfamiView protein in Pfam
PF06849 DUF1246, 1 hit
PF06973 DUF1297, 1 hit
PIRSFiPIRSF004602 ATPgrasp_PurP, 1 hit
SUPFAMiSSF52440 SSF52440, 1 hit
PROSITEiView protein in PROSITE
PS50975 ATP_GRASP, 1 hit

Sequencei

Sequence statusi: Complete.

Q9V0I8-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MVKIVVRIAT YASHSALQIL KGAKDEGFET IAFGSERVKP LYTKYFPVAD
60 70 80 90 100
YFLVGKYPED ELLELNAVVI PTGSFVAHLG VELVERMKVP YFGNKRVLKW
110 120 130 140 150
ESDRNLERKW LEKAKLKLPR VYDDPDDIDR PVIVKPHGAK GGRGYFIAKD
160 170 180 190 200
PQDFWTKVEK FLGIKDKEDL KNVQIQEYVI GVPVYPHYFY SKLTRELELM
210 220 230 240 250
SIDRRYESNV DAIGRIPSKD QLELELDITY TVIGNIPLVL RESLLMDVIE
260 270 280 290 300
AGERTVKAAE ELMGGLWGPF CLEGVFTPDL DFVVFEISAR IVAGTNPFIN
310 320 330
GSPYTWLKYD EPMSTGRRIA REIRLAIEED KLDEVVS
Length:337
Mass (Da):38,529
Last modified:May 1, 2000 - v1
Checksum:i0876B8E0D865C1C9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ248285 Genomic DNA Translation: CAB49715.1
HE613800 Genomic DNA Translation: CCE70201.1
PIRiB75125

Genome annotation databases

EnsemblBacteriaiCAB49715; CAB49715; PAB0547
KEGGipab:PAB0547
PATRICifig|272844.11.peg.844

Similar proteinsi

Entry informationi

Entry nameiPURP_PYRAB
AccessioniPrimary (citable) accession number: Q9V0I8
Secondary accession number(s): G8ZH06
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: May 1, 2000
Last modified: March 28, 2018
This is version 98 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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