MCM

Functionⁱ

Presumptive replicative helicase. Has ATPase and DNA helicase activities. The latter preferentially melts 5'-tailed oligonucleotides and is stimulated by the SSB protein (single-stranded DNA binding protein). The active ATPase sites in the MCM ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The helicase function is proposed to use a partially sequential mode of ATP hydrolysis; the complex appears to tolerate multiple catalytically inactive subunits.1 Publication

Catalytic activityⁱ

ATP + H₂O = ADP + phosphate.2 Publications

Activity regulationⁱ

Inhibited by the cdc6-2 protein. The ATPase activity is not stimulated in the presence of DNA molecules.

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Nucleotide bindingⁱ	340 – 347	ATPSequence analysis		8

GO - Molecular functionⁱ

ATP binding Source: UniProtKB-KW
DNA binding Source: UniProtKB-KW
helicase activity Source: UniProtKB-KW
identical protein binding
Source: IntAct
Inferred from physical interactionⁱ

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

DNA replication initiation Source: InterPro

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	DNA-binding, Helicase, Hydrolase
Biological process	DNA replication
Ligand	ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAⁱ	3.6.4.12 6163

BRENDAⁱ

3.6.4.12 6163

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Minichromosome maintenance protein MCM (EC:3.6.4.12)
Gene namesⁱ	Name:MCM Ordered Locus Names:SSO0774
Organismⁱ	Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Taxonomic identifierⁱ	273057 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Archaea › TACK group › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Sulfolobus › Sulfolobus solfataricus
Proteomesⁱ	UP000001974 Componentⁱ: Chromosome

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Length
Mutagenesisⁱ	189	L → D: Predominantly monomeric and loss of helicase activity; when associated with R-191. 1 Publication	1
Mutagenesisⁱ	191	D → R: Predominantly monomeric and loss of helicase activity; when associated with D-189. 1 Publication	1
Mutagenesisⁱ	202 – 204	EEV → GGG: Loss of helicase activity. 1 Publication	3
Mutagenesisⁱ	318	F → A: No effect on helicase and ATPase activity. 1 Publication	1
Mutagenesisⁱ	326 – 327	ED → AA: Impairs helicase activity; when associated with A-329. 1 Publication	2
Mutagenesisⁱ	329	R → A: Impairs helicase activity; when associated with 326-A-A-327. 1 Publication	1
Mutagenesisⁱ	331	R → A: Loss of helicase and ATPase activity. 1 Publication	1
Mutagenesisⁱ	346	K → A: Loss of helicase and ATPase activity. 2 Publications	1
Mutagenesisⁱ	346	K → A: Sharp decrease in ATPase activity. Almost devoid of helicase activity. 2 Publications	1
Mutagenesisⁱ	359	R → A: Loss of helicase and reduction of ATPase activity. 1 Publication	1
Mutagenesisⁱ	366	K → E: Loss of helicase and reduction of ATPase activity. 1 Publication	1
Mutagenesisⁱ	374	T → E: Reduction of helicase and gain of ATPase activity. 1 Publication	1
Mutagenesisⁱ	404	D → A: Loss of helicase and ATPase activity. 1 Publication	1
Mutagenesisⁱ	416	A → R: Predominantly monomeric and loss of helicase activity; when associated with R-420. 1 Publication	1
Mutagenesisⁱ	420	A → R: Predominantly monomeric and loss of helicase activity; when associated with R-416. 1 Publication	1
Mutagenesisⁱ	423 – 424	QQ → AA: Loss of helicase and ATPase activity. 1 Publication	2
Mutagenesisⁱ	448	N → A: Loss of helicase and ATPase activity. 1 Publication	1
Mutagenesisⁱ	473	R → A: Loss of helicase and ATPase activity. 1 Publication	1
Mutagenesisⁱ	488	D → A: No effect on helicase and reduction of ATPase activity. 1 Publication	1
Mutagenesisⁱ	550 – 554	TPDSP → GGGGG: Predominantly monomeric and loss of helicase activity. 1 Publication	5
Mutagenesisⁱ	555 – 557	ILI → DSD: Exclusively monomeric and loss of helicase activity, ATPase activity and DNA binding. 1 Publication	3
Mutagenesisⁱ	560	R → A: Loss of helicase and ATPase activity. 1 Publication	1

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000194128	1 – 686	Minichromosome maintenance protein MCMAdd BLAST		686

Interactionⁱ

Subunit structureⁱ

Homohexamers. Forms a complex with SSB.2 Publications

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
itself		9	EBI-8081298,EBI-8081298
ORF-c40_009	Q9UXG0	5	EBI-8081298,EBI-8081318	From Sulfolobus solfataricus.

GO - Molecular functionⁱ

identical protein binding
Source: IntAct
Inferred from physical interactionⁱ

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

Database of interacting proteins More...DIPⁱ	DIP-42884N
IntActⁱ	Q9UXG1, 1 interactor
Molecular INTeraction database More...MINTⁱ	Q9UXG1
STRINGⁱ	273057.SSO0774

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Helixⁱ	10 – 20	Combined sources	11
Beta strandⁱ	24 – 27	Combined sources	4
Helixⁱ	29 – 39	Combined sources	11
Beta strandⁱ	43 – 48	Combined sources	6
Helixⁱ	49 – 55	Combined sources	7
Helixⁱ	57 – 65	Combined sources	9
Helixⁱ	67 – 85	Combined sources	19
Helixⁱ	89 – 92	Combined sources	4
Beta strandⁱ	97 – 102	Combined sources	6
Helixⁱ	109 – 111	Combined sources	3
Helixⁱ	114 – 116	Combined sources	3
Beta strandⁱ	119 – 130	Combined sources	12
Beta strandⁱ	134 – 139	Combined sources	6
Beta strandⁱ	141 – 145	Combined sources	5
Turnⁱ	147 – 149	Combined sources	3
Beta strandⁱ	152 – 156	Combined sources	5
Turnⁱ	172 – 174	Combined sources	3
Beta strandⁱ	179 – 182	Combined sources	4
Helixⁱ	184 – 186	Combined sources	3
Beta strandⁱ	187 – 198	Combined sources	12
Helixⁱ	201 – 203	Combined sources	3
Beta strandⁱ	212 – 218	Combined sources	7
Helixⁱ	219 – 221	Combined sources	3
Beta strandⁱ	230 – 240	Combined sources	11
Beta strandⁱ	251 – 264	Combined sources	14
Helixⁱ	612 – 615	Combined sources	4
Helixⁱ	621 – 637	Combined sources	17
Beta strandⁱ	640 – 644	Combined sources	5
Helixⁱ	645 – 655	Combined sources	11
Helixⁱ	659 – 672	Combined sources	14
Beta strandⁱ	674 – 679	Combined sources	6
Beta strandⁱ	682 – 685	Combined sources	4

Show more details

3D structure databases

ProteinModelPortalⁱ	Q9UXG1
SMRⁱ	Q9UXG1
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ	Q9UXG1

EvolutionaryTraceⁱ

Q9UXG1

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	291 – 495	MCMAdd BLAST		205

Motif

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Motifⁱ	472 – 475	Arginine finger		4

Sequence similaritiesⁱ

Belongs to the MCM family.Curated

Phylogenomic databases

eggNOGⁱ	arCOG00439 Archaea COG1241 LUCA
HOGENOMⁱ	HOG000224127
InParanoidⁱ	Q9UXG1
KEGG Orthology (KO) More...KOⁱ	K10726
OMAⁱ	IHKVLQL
Database of Orthologous Groups More...OrthoDBⁱ	POG093Z00UV

Family and domain databases

Gene3Dⁱ	1.10.10.10, 1 hit
InterProⁱ	View protein in InterPro IPR003593 AAA+_ATPase IPR031327 MCM IPR001208 MCM_dom IPR027925 MCM_N IPR033762 MCM_OB IPR012340 NA-bd_OB-fold IPR027417 P-loop_NTPase IPR036388 WH-like_DNA-bd_sf
PANTHERⁱ	PTHR11630 PTHR11630, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00493 MCM, 1 hit PF14551 MCM_N, 1 hit PF17207 MCM_OB, 1 hit
PRINTSⁱ	PR01657 MCMFAMILY
SMARTⁱ	View protein in SMART SM00382 AAA, 1 hit SM00350 MCM, 1 hit
SUPFAMⁱ	SSF50249 SSF50249, 1 hit SSF52540 SSF52540, 1 hit
PROSITEⁱ	View protein in PROSITE PS50051 MCM_2, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Q9UXG1-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MEIPSKQIDY RDVFIEFLTT FKGNNNQNKY IERINELVAY RKKSLIIEFS 
        60         70         80         90        100
DVLSFNENLA YEIINNTKII LPILEGALYD HILQLDPTYQ RDIEKVHVRI 
       110        120        130        140        150
VGIPRVIELR KIRSTDIGKL ITIDGILVKV TPVKERIYKA TYKHIHPDCM 
       160        170        180        190        200
QEFEWPEDEE MPEVLEMPTI CPKCGKPGQF RLIPEKTKLI DWQKAVIQER 
       210        220        230        240        250
PEEVPSGQLP RQLEIILEDD LVDSARPGDR VKVTGILDIK QDSPVKRGSR 
       260        270        280        290        300
AVFDIYMKVS SIEVSQKVLD EVIISEEDEK KIKDLAKDPW IRDRIISSIA 
       310        320        330        340        350
PSIYGHWELK EALALALFGG VPKVLEDTRI RGDIHILIIG DPGTAKSQML 
       360        370        380        390        400
QFISRVAPRA VYTTGKGSTA AGLTAAVVRE KGTGEYYLEA GALVLADGGI 
       410        420        430        440        450
AVIDEIDKMR DEDRVAIHEA MEQQTVSIAK AGIVAKLNAR AAVIAAGNPK 
       460        470        480        490        500
FGRYISERPV SDNINLPPTI LSRFDLIFIL KDQPGEQDRE LANYILDVHS 
       510        520        530        540        550
GKSTKNIIDI DTLRKYIAYA RKYVTPKITS EAKNLITDFF VEMRKKSSET 
       560        570        580        590        600
PDSPILITPR QLEALIRISE AYAKMALKAE VTREDAERAI NIMRLFLESV 
       610        620        630        640        650
GVDMESGKID IDTIMTGKPK SAREKMMKII EIIDSLAVSS ECAKVKDILK 
       660        670        680 
EAQQVGIEKS NIEKLLTDMR KSGIIYEAKP ECYKKV

Length:686

Mass (Da):77,428

Last modified:May 1, 2000 - v1

Checksum:ⁱC3ECD380F272E77E

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	Y18930 Genomic DNA Translation: CAB57529.1 AE006641 Genomic DNA Translation: AAK41071.1
PIRⁱ	H90226
NCBI Reference Sequences More...RefSeqⁱ	WP_009991364.1, NC_002754.1

Genome annotation databases

EnsemblBacteriaⁱ	AAK41071; AAK41071; SSO0774
GeneIDⁱ	27427101
KEGGⁱ	sso:SSO0774
PATRICⁱ	fig\|273057.12.peg.774

Protein	Similar proteins		Species	Score	Length	Source
Q9UXG1	Minichromosome maintenance protein MCM		SULSF		686	UniRef100_Q9UXG1
MCM family protein		SULS9		686
UPI0002B8CC1E		SULSF		692
UPI000181D049		SULSF		268
UPI00018A86A0		SULSF		595

Protein	Similar proteins		Species	Score	Length	Source
Q9UXG1	Minichromosome maintenance protein MCM		SULSF		686	UniRef90_Q9UXG1
MCM family protein		SULS9		686
MCM family protein		SULIY		686
Putative ATPase involved in replication control, Cdc46/Mcm family		Sulfolobus islandicus LAL14/1		686
Minichromosome maintenance protein MCM		SULIS		686
+11

Protein	Similar proteins		Species	Score	Length	Source
Q9UXG1	Minichromosome maintenance protein MCM		SULSF		686	UniRef50_Q9UXG1
MCM family protein		SULS9		686
Minichromosome maintenance protein MCM		SULIS		686
Putative ATPase involved in replication control, Cdc46/Mcm family		Sulfolobus islandicus LAL14/1		686
MCM family protein		SULID		686
+12

Entry informationⁱ

Entry nameⁱ	MCM_SULSO
Accessionⁱ	Q9UXG1Primary (citable) accession number: Q9UXG1
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	March 29, 2005
	Last sequence update:	May 1, 2000
	Last modified:	September 12, 2018
	This is version 109 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

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Supporting data

UniProtKB - Q9UXG1 (MCM_SULSO)

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Minichromosome maintenance protein MCM

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Motif

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ