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Protein

Alkyltransferase-like protein 1

Gene

atl1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in DNA damage recognition. Binds DNA containing O6-methylguanine and larger O6-alkylguanine adducts. The DNA is bent, the damaged base is rotated out of the DNA duplex into a hydrophobic binding pocket (nucleotide flipping), with Arg-39 donating a hydrogen bond to the orphaned cytosine to stabilize the extrahelical DNA conformation. This structural change in DNA presents the lesion to the nucleotide excision repair (NER) pathway (PubMed:16679453, PubMed:19516334, PubMed:23112169). The affinity for O6-alkylguanine adducts increases with the size of the alkyl group. Low affinity small O6-alkylguanines are directed to the global genome repair pathway of NER via rhp7-rhp16 and rhp41-rhp23, while strong binding to bulky O6-alkylguanines stalls the transcription machinery and diverts the damage to the transcription-coupled repair pathway of NER via rhp26 (PubMed:22658721).4 Publications

Miscellaneous

Does not have alkyltransferase activity. A tryptophan residue replaces the cysteine at the known active site of MGMT.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei25Required for phosphate rotation/nucleotide flipping1 Publication1
Sitei39Arg finger, required for nucleotide flipping1 Publication1
Sitei69Critical for recognition of O(6)-alkylguanines, probes the electrostatic potential of the flipped base to distinguish between O(6)-alkylguanine and guanine1 Publication1

GO - Molecular functioni

  • catalytic activity Source: InterPro
  • damaged DNA binding Source: PomBase
  • O6-alkylguanine-DNA binding Source: PomBase

GO - Biological processi

Keywordsi

Molecular functionDNA-binding
Biological processDNA damage, DNA repair

Names & Taxonomyi

Protein namesi
Recommended name:
Alkyltransferase-like protein 11 Publication
Gene namesi
Name:atl11 Publication
ORF Names:SPAC1250.04cImported
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC1250.04c
PomBaseiSPAC1250.04c atl1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi69R → A or F: Reduces discrimination of modified bases 10-100-fold and increases sensitivity toward alkylating agents. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002492371 – 108Alkyltransferase-like protein 1Add BLAST108

Proteomic databases

MaxQBiQ9UTN9
PaxDbiQ9UTN9
PRIDEiQ9UTN9

Interactioni

Protein-protein interaction databases

BioGridi278199, 18 interactors
DIPiDIP-60450N
IntActiQ9UTN9, 1 interactor
STRINGi4896.SPAC1250.04c.1

Structurei

Secondary structure

1108
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9UTN9
SMRiQ9UTN9
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9UTN9

Family & Domainsi

Sequence similaritiesi

Belongs to the MGMT family. ATL subfamily.Curated

Phylogenomic databases

HOGENOMiHOG000244138
InParanoidiQ9UTN9
KOiK07443
OMAiWHRVINA
OrthoDBiEOG092C5L5L
PhylomeDBiQ9UTN9

Family and domain databases

CDDicd06445 ATase, 1 hit
Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR014048 MethylDNA_cys_MeTrfase_DNA-bd
IPR036217 MethylDNA_cys_MeTrfase_DNAb
IPR036388 WH-like_DNA-bd_sf
PfamiView protein in Pfam
PF01035 DNA_binding_1, 1 hit
SUPFAMiSSF46767 SSF46767, 1 hit
TIGRFAMsiTIGR00589 ogt, 1 hit

Sequencei

Sequence statusi: Complete.

Q9UTN9-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MRMDEFYTKV YDAVCEIPYG KVSTYGEIAR YVGMPSYARQ VGQAMKHLHP
60 70 80 90 100
ETHVPWHRVI NSRGTISKRD ISAGEQRQKD RLEEEGVEIY QTSLGEYKLN

LPEYMWKP
Length:108
Mass (Da):12,670
Last modified:May 1, 2000 - v1
Checksum:i7F8B3BA12CED07F1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA Translation: CAB54827.1
PIRiT37558
RefSeqiNP_594858.1, NM_001020287.2

Genome annotation databases

EnsemblFungiiSPAC1250.04c.1; SPAC1250.04c.1:pep; SPAC1250.04c
GeneIDi2541704
KEGGispo:SPAC1250.04c

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA Translation: CAB54827.1
PIRiT37558
RefSeqiNP_594858.1, NM_001020287.2

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3GVAX-ray2.00A/B1-108[»]
3GX4X-ray2.70X1-108[»]
3GYHX-ray2.80X1-108[»]
4ENJX-ray3.10A1-108[»]
4ENKX-ray3.04A1-108[»]
4ENMX-ray2.84A1-108[»]
4ENNX-ray2.84A/B1-108[»]
4HDUX-ray2.85A1-108[»]
4HDVX-ray2.70A1-108[»]
ProteinModelPortaliQ9UTN9
SMRiQ9UTN9
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi278199, 18 interactors
DIPiDIP-60450N
IntActiQ9UTN9, 1 interactor
STRINGi4896.SPAC1250.04c.1

Proteomic databases

MaxQBiQ9UTN9
PaxDbiQ9UTN9
PRIDEiQ9UTN9

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC1250.04c.1; SPAC1250.04c.1:pep; SPAC1250.04c
GeneIDi2541704
KEGGispo:SPAC1250.04c

Organism-specific databases

EuPathDBiFungiDB:SPAC1250.04c
PomBaseiSPAC1250.04c atl1

Phylogenomic databases

HOGENOMiHOG000244138
InParanoidiQ9UTN9
KOiK07443
OMAiWHRVINA
OrthoDBiEOG092C5L5L
PhylomeDBiQ9UTN9

Miscellaneous databases

EvolutionaryTraceiQ9UTN9
PROiPR:Q9UTN9

Family and domain databases

CDDicd06445 ATase, 1 hit
Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR014048 MethylDNA_cys_MeTrfase_DNA-bd
IPR036217 MethylDNA_cys_MeTrfase_DNAb
IPR036388 WH-like_DNA-bd_sf
PfamiView protein in Pfam
PF01035 DNA_binding_1, 1 hit
SUPFAMiSSF46767 SSF46767, 1 hit
TIGRFAMsiTIGR00589 ogt, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiATL1_SCHPO
AccessioniPrimary (citable) accession number: Q9UTN9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: May 1, 2000
Last modified: September 12, 2018
This is version 103 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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