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UniProtKB - Q9UT61 (MAN1_SCHPO)
Protein
Alpha-mannosidase
Gene
ams1
Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Functioni
Degrades free oligosaccharides in the vacuole.
By similarityCatalytic activityi
- Hydrolysis of terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides. EC:3.2.1.24
Cofactori
Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 290 | ZincBy similarity | 1 | |
Metal bindingi | 292 | ZincBy similarity | 1 | |
Active sitei | 402 | NucleophileBy similarity | 1 | |
Metal bindingi | 402 | ZincBy similarity | 1 | |
Metal bindingi | 615 | ZincBy similarity | 1 |
GO - Molecular functioni
- alpha-mannosidase activity Source: GO_Central
- carbohydrate binding Source: InterPro
- mannosyl-oligosaccharide 1,6-alpha-mannosidase activity Source: PomBase
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- mannose metabolic process Source: InterPro
- oligosaccharide catabolic process Source: GO_Central
Keywordsi
Molecular function | Glycosidase, Hydrolase |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-SPO-8853383, Lysosomal oligosaccharide catabolism |
Protein family/group databases
CAZyi | GH38, Glycoside Hydrolase Family 38 |
Names & Taxonomyi
Protein namesi | Recommended name: Alpha-mannosidase (EC:3.2.1.24)Alternative name(s): Alpha-D-mannoside mannohydrolase |
Gene namesi | Name:ams1 Synonyms:mns2 ORF Names:SPAC513.05 |
Organismi | Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) |
Taxonomic identifieri | 284812 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › |
Proteomesi |
|
Organism-specific databases
PomBasei | SPAC513.05, ams1 |
VEuPathDBi | FungiDB:SPAC513.05 |
Subcellular locationi
Cytosol
- cytosol Source: PomBase
Vacuole
- fungal-type vacuole lumen Source: PomBase
- fungal-type vacuole membrane Source: PomBase
Keywords - Cellular componenti
Cytoplasm, VacuolePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000363389 | 1 – 1077 | Alpha-mannosidaseAdd BLAST | 1077 |
Proteomic databases
MaxQBi | Q9UT61 |
PaxDbi | Q9UT61 |
PTM databases
iPTMneti | Q9UT61 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 279905, 1 interactor |
STRINGi | 4896.SPAC513.05.1 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q9UT61 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Belongs to the glycosyl hydrolase 38 family.Curated
Phylogenomic databases
eggNOGi | KOG4342, Eukaryota |
HOGENOMi | CLU_003442_0_1_1 |
InParanoidi | Q9UT61 |
OMAi | GQYWDAW |
PhylomeDBi | Q9UT61 |
Family and domain databases
Gene3Di | 1.20.1270.50, 1 hit 3.20.110.10, 1 hit |
InterProi | View protein in InterPro IPR011013, Gal_mutarotase_sf_dom IPR041147, GH38_C IPR011330, Glyco_hydro/deAcase_b/a-brl IPR011682, Glyco_hydro_38_C IPR015341, Glyco_hydro_38_cen IPR037094, Glyco_hydro_38_cen_sf IPR000602, Glyco_hydro_38_N IPR027291, Glyco_hydro_38_N_sf IPR028995, Glyco_hydro_57/38_cen_sf |
Pfami | View protein in Pfam PF09261, Alpha-mann_mid, 1 hit PF17677, Glyco_hydro38C2, 1 hit PF07748, Glyco_hydro_38C, 1 hit PF01074, Glyco_hydro_38N, 1 hit |
SMARTi | View protein in SMART SM00872, Alpha-mann_mid, 1 hit |
SUPFAMi | SSF74650, SSF74650, 1 hit SSF88688, SSF88688, 1 hit SSF88713, SSF88713, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9UT61-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTLFPVLNNT PVGKQVDSIY ESRLDQFLSE GQYRDFNLPS VYDHARIDNP
60 70 80 90 100
SGDVNNDLSK GFVDLKVYRV PDLSRPSFNE VVGHKKFDET ASKGDTFGPS
110 120 130 140 150
WATFWFEVHI RLPKSWAKYE QVIFQWNCDN EGLVYSQDGV PLQAFSGSER
160 170 180 190 200
TDFILPDSWK TTEDTFYIEM ACNGMFGTGA GSQIAPPDPN RYFTLTKADL
210 220 230 240 250
VAPNLPAMAL AYDFLLMQQC VKQLPSNCWQ KYKARQICND IMNTFHPNDL
260 270 280 290 300
STINECRNLA KAFLGNDIDS EAVFEKNNDK ANVFAIGHCH IDTAWLWPFA
310 320 330 340 350
ETRRKIVRSW ATQMNIMDRY PEYQFVCSQA LQYLWLKEDH PDVFEKLKEY
360 370 380 390 400
VNQNKFIPIG GSWVEHDTNI PNGESLIRQF LLGQHFFEKE FGVRCRTFWL
410 420 430 440 450
PDTFGYSSQI PQICRLCGMD RFLTQKLSWN NINSFPTSTF NWVALDGSQV
460 470 480 490 500
ICHMPPANTY TADTNVNDVL HSIDQHKNLV NDQAGLLVFG IGDGGGGPTP
510 520 530 540 550
EMLEKLRRCK GIANTVGYLP NVKLGNTVDE FFDGILKRTN AGQTLPSWNG
560 570 580 590 600
ELYFEFHRGT YTTQAELKKL MRKVEIALHD AEYVSTLASI FSKDYSYPKE
610 620 630 640 650
SLQDLWRDTL LCQFHDVLPG SCIEMVYKDA IPIMSKVLKN TEALLWQAIE
660 670 680 690 700
QLGFKKASSS DNKEQLCLLN TLPWNVRGVI TETEENKLVY FESCDGKGIL
710 720 730 740 750
TAAHTSLKHP AAAYQKDDNF ILVNDHLRVT IAPNGLILSL FDLHKEREIL
760 770 780 790 800
DLKSGKNHAG ANQYVLFEDT PLSWQAWDTE VFSLEKYEVL DKGKVSIKES
810 820 830 840 850
GPLRASVVVD IPISELSHMK ATISLEGYND CSEFTGVNFT CEVDWHESCK
860 870 880 890 900
FLKVEFPVDI HSEFASYETQ FGITKRPTHY NTSWDVAKFE VCHQKFADYS
910 920 930 940 950
DFTYGVSVLN DCKYGFSTHG NLMRLSLLRS PKQPDAHADM GKHTIRYAVY
960 970 980 990 1000
PHSKPLDSST VRAAHKFNSN FRLLTRASDT ANLDIFDAFQ LVGEPNVILS
1010 1020 1030 1040 1050
HIKMAEKGKS IILRVYESLG GKSRARLVIK SLTVASVTKC NGLEEDLEEL
1060 1070
CTLKSNDYYE VPIELRAFEI ATFKVNL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CU329670 Genomic DNA Translation: CAB58728.1 |
PIRi | T38900 |
RefSeqi | NP_593979.1, NM_001019405.2 |
Genome annotation databases
EnsemblFungii | SPAC513.05.1; SPAC513.05.1:pep; SPAC513.05 |
GeneIDi | 2543485 |
KEGGi | spo:SPAC513.05 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CU329670 Genomic DNA Translation: CAB58728.1 |
PIRi | T38900 |
RefSeqi | NP_593979.1, NM_001019405.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6LZ1 | electron microscopy | 3.20 | A/B/C/D | 1-1077 | [»] | |
7DD9 | electron microscopy | 2.40 | A/C/E/G | 1-1077 | [»] | |
SMRi | Q9UT61 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 279905, 1 interactor |
STRINGi | 4896.SPAC513.05.1 |
Protein family/group databases
CAZyi | GH38, Glycoside Hydrolase Family 38 |
PTM databases
iPTMneti | Q9UT61 |
Proteomic databases
MaxQBi | Q9UT61 |
PaxDbi | Q9UT61 |
Genome annotation databases
EnsemblFungii | SPAC513.05.1; SPAC513.05.1:pep; SPAC513.05 |
GeneIDi | 2543485 |
KEGGi | spo:SPAC513.05 |
Organism-specific databases
PomBasei | SPAC513.05, ams1 |
VEuPathDBi | FungiDB:SPAC513.05 |
Phylogenomic databases
eggNOGi | KOG4342, Eukaryota |
HOGENOMi | CLU_003442_0_1_1 |
InParanoidi | Q9UT61 |
OMAi | GQYWDAW |
PhylomeDBi | Q9UT61 |
Enzyme and pathway databases
Reactomei | R-SPO-8853383, Lysosomal oligosaccharide catabolism |
Miscellaneous databases
PROi | PR:Q9UT61 |
Family and domain databases
Gene3Di | 1.20.1270.50, 1 hit 3.20.110.10, 1 hit |
InterProi | View protein in InterPro IPR011013, Gal_mutarotase_sf_dom IPR041147, GH38_C IPR011330, Glyco_hydro/deAcase_b/a-brl IPR011682, Glyco_hydro_38_C IPR015341, Glyco_hydro_38_cen IPR037094, Glyco_hydro_38_cen_sf IPR000602, Glyco_hydro_38_N IPR027291, Glyco_hydro_38_N_sf IPR028995, Glyco_hydro_57/38_cen_sf |
Pfami | View protein in Pfam PF09261, Alpha-mann_mid, 1 hit PF17677, Glyco_hydro38C2, 1 hit PF07748, Glyco_hydro_38C, 1 hit PF01074, Glyco_hydro_38N, 1 hit |
SMARTi | View protein in SMART SM00872, Alpha-mann_mid, 1 hit |
SUPFAMi | SSF74650, SSF74650, 1 hit SSF88688, SSF88688, 1 hit SSF88713, SSF88713, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MAN1_SCHPO | |
Accessioni | Q9UT61Primary (citable) accession number: Q9UT61 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 10, 2009 |
Last sequence update: | May 1, 2000 | |
Last modified: | February 23, 2022 | |
This is version 120 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Schizosaccharomyces pombe
Schizosaccharomyces pombe: entries and gene names - Glycosyl hydrolases
Classification of glycosyl hydrolase families and list of entries - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families