Swi5-dependent recombination DNA repair protein 1

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functionⁱ

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functionⁱ

• ATPase activator activity Source: PomBase <p>Inferred from Direct Assay</p> <p>Used to indicate a direct assay for the function, process or component indicated by the GO term.</p> <p>More information in the <a href="http://geneontology.org/page/guide-go-evidence-codes#ida">GO evidence code guide</a></p> Inferred from direct assayⁱ
  • "The Swi5-Sfr1 complex stimulates Rhp51/Rad51- and Dmc1-mediated DNA strand exchange in vitro."
    Haruta N., Kurokawa Y., Murayama Y., Akamatsu Y., Unzai S., Tsutsui Y., Iwasaki H.
    Nat Struct Mol Biol 13:823-830(2006) [PubMed] [Europe PMC] [Abstract]
  • "Cooperative interactions facilitate stimulation of Rad51 by the Swi5-Sfr1 auxiliary factor complex."
    Argunhan B., Sakakura M., Afshar N., Kurihara M., Ito K., Maki T., Kanamaru S., Murayama Y., Tsubouchi H., Takahashi M., Takahashi H., Iwasaki H.
    Elife 9:e52566-e52566(2020) [PubMed] [Europe PMC] [Abstract]
• double-stranded DNA binding Source: PomBaseInferred from direct assayⁱ
  • "Mechanistic insights into the activation of Rad51-mediated strand exchange from the structure of a recombination activator, the Swi5-Sfr1 complex."
    Kuwabara N., Murayama Y., Hashimoto H., Kokabu Y., Ikeguchi M., Sato M., Mayanagi K., Tsutsui Y., Iwasaki H., Shimizu T.
    Structure 20:440-449(2012) [PubMed] [Europe PMC] [Abstract]
• single-stranded DNA binding Source: PomBaseInferred from direct assayⁱ
  • "Mechanistic insights into the activation of Rad51-mediated strand exchange from the structure of a recombination activator, the Swi5-Sfr1 complex."
    Kuwabara N., Murayama Y., Hashimoto H., Kokabu Y., Ikeguchi M., Sato M., Mayanagi K., Tsutsui Y., Iwasaki H., Shimizu T.
    Structure 20:440-449(2012) [PubMed] [Europe PMC] [Abstract]

GO - Biological processⁱ

• chromosome segregation Source: UniProtKB-KW
• DNA recombinase assembly Source: PomBaseInferred from direct assayⁱ
  • "The Swi5-Sfr1 complex stimulates Rhp51/Rad51- and Dmc1-mediated DNA strand exchange in vitro."
    Haruta N., Kurokawa Y., Murayama Y., Akamatsu Y., Unzai S., Tsutsui Y., Iwasaki H.
    Nat Struct Mol Biol 13:823-830(2006) [PubMed] [Europe PMC] [Abstract]
  • "Mechanistic insights into the activation of Rad51-mediated strand exchange from the structure of a recombination activator, the Swi5-Sfr1 complex."
    Kuwabara N., Murayama Y., Hashimoto H., Kokabu Y., Ikeguchi M., Sato M., Mayanagi K., Tsutsui Y., Iwasaki H., Shimizu T.
    Structure 20:440-449(2012) [PubMed] [Europe PMC] [Abstract]
• DNA recombination Source: GO_Central <p>Inferred from Biological aspect of Ancestor</p> <p>A type of phylogenetic evidence whereby an aspect of a descendent is inferred through the characterization of an aspect of a ancestral gene.</p> <p>More information in the <a href="http://geneontology.org/page/guide-go-evidence-codes#iba">GO evidence code guide</a></p> Inferred from biological aspect of ancestorⁱ
  • "Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium."
    Gaudet P., Livstone M.S., Lewis S.E., Thomas P.D.
    Brief Bioinform 12:449-462(2011) [PubMed] [Europe PMC] [Abstract]
• double-strand break repair involved in meiotic recombination Source: PomBase <p>Inferred from Mutant Phenotype</p> <p>Describes annotations that are concluded from looking at variations or changes in a gene product such as mutations or abnormal levels and includes techniques such as knockouts, overexpression, anti-sense experiments and use of specific protein inhibitors.</p> <p>More information in the <a href="http://geneontology.org/page/guide-go-evidence-codes#imp">GO evidence code guide</a></p> Inferred from mutant phenotypeⁱ
  • "Genetic analysis reveals different roles of Schizosaccharomyces pombe sfr1/dds20 in meiotic and mitotic DNA recombination and repair."
    Khasanov F.K., Salakhova A.F., Khasanova O.S., Grishchuk A.L., Chepurnaja O.V., Korolev V.G., Kohli J., Bashkirov V.I.
    Curr Genet 54:197-211(2008) [PubMed] [Europe PMC] [Abstract]
• double-strand break repair via homologous recombination Source: PomBase <p>Inferred from Genetic Interaction</p> <p>Used to describe "traditional" genetic interactions such as suppressors and synthetic lethals as well as other techniques such as functional complementation, rescue experiments, or inferences about a gene drawn from the phenotype of a mutation in a different gene.</p> <p>More information in the <a href="http://geneontology.org/page/guide-go-evidence-codes#igi">GO evidence code guide</a></p> Inferred from genetic interactionⁱ
  • Ref.1

    "Two different Swi5-containing protein complexes are involved in mating-type switching and recombination repair in fission yeast."
    Akamatsu Y., Dziadkowiec D., Ikeguchi M., Shinagawa H., Iwasaki H.
    Proc. Natl. Acad. Sci. U.S.A. 100:15770-15775(2003) [PubMed] [Europe PMC] [Abstract]

    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INTERACTION WITH SWI5 AND RHP51.
  • "Genetic analysis reveals different roles of Schizosaccharomyces pombe sfr1/dds20 in meiotic and mitotic DNA recombination and repair."
    Khasanov F.K., Salakhova A.F., Khasanova O.S., Grishchuk A.L., Chepurnaja O.V., Korolev V.G., Kohli J., Bashkirov V.I.
    Curr Genet 54:197-211(2008) [PubMed] [Europe PMC] [Abstract]
• meiotic DNA recombinase assembly involved in reciprocal meiotic recombination Source: PomBaseInferred from direct assayⁱ
  • "Dual regulation of Dmc1-driven DNA strand exchange by Swi5-Sfr1 activation and Rad22 inhibition."
    Murayama Y., Kurokawa Y., Tsutsui Y., Iwasaki H.
    Genes Dev 27:2299-2304(2013) [PubMed] [Europe PMC] [Abstract]
• meiotic joint molecule formation Source: PomBaseInferred from direct assayⁱ
  • "Two auxiliary factors promote Dmc1-driven DNA strand exchange via stepwise mechanisms."
    Tsubouchi H., Argunhan B., Ito K., Takahashi M., Iwasaki H.
    Proc Natl Acad Sci U S A 117:12062-12070(2020) [PubMed] [Europe PMC] [Abstract]
• meiotic strand invasion Source: PomBaseInferred from direct assayⁱ
  • "Two three-strand intermediates are processed during Rad51-driven DNA strand exchange."
    Ito K., Murayama Y., Takahashi M., Iwasaki H.
    Nat Struct Mol Biol 25:29-36(2018) [PubMed] [Europe PMC] [Abstract]
• mitotic intra-S DNA damage checkpoint signaling Source: PomBaseInferred from mutant phenotypeⁱ
  • "Mus81, Rhp51(Rad51), and Rqh1 form an epistatic pathway required for the S-phase DNA damage checkpoint."
    Willis N., Rhind N.
    Mol Biol Cell 20:819-833(2009) [PubMed] [Europe PMC] [Abstract]
• negative regulation of DNA recombinase disassembly Source: PomBaseInferred from direct assayⁱ
  • "Swi5-Sfr1 stimulates Rad51 recombinase filament assembly by modulating Rad51 dissociation."
    Lu C.H., Yeh H.Y., Su G.C., Ito K., Kurokawa Y., Iwasaki H., Chi P., Li H.W.
    Proc Natl Acad Sci U S A 115:E10059-E10068(2018) [PubMed] [Europe PMC] [Abstract]
• reciprocal meiotic recombination Source: PomBaseInferred from mutant phenotypeⁱ
  • "Rad51/Dmc1 paralogs and mediators oppose DNA helicases to limit hybrid DNA formation and promote crossovers during meiotic recombination."
    Lorenz A., Mehats A., Osman F., Whitby M.C.
    Nucleic Acids Res 42:13723-13735(2014) [PubMed] [Europe PMC] [Abstract]
• strand invasion Source: PomBaseInferred from direct assayⁱ
  • "Multiple regulation of Rad51-mediated homologous recombination by fission yeast Fbh1."
    Tsutsui Y., Kurokawa Y., Ito K., Siddique M.S., Kawano Y., Yamao F., Iwasaki H.
    PLoS Genet 10:e1004542-e1004542(2014) [PubMed] [Europe PMC] [Abstract]
  • "Cooperative interactions facilitate stimulation of Rad51 by the Swi5-Sfr1 auxiliary factor complex."
    Argunhan B., Sakakura M., Afshar N., Kurihara M., Ito K., Maki T., Kanamaru S., Murayama Y., Tsubouchi H., Takahashi M., Takahashi H., Iwasaki H.
    Elife 9:e52566-e52566(2020) [PubMed] [Europe PMC] [Abstract]

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsⁱ

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyⁱ

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationⁱ

• UniProt annotation
• GO - Cellular component

Keywords - Cellular componentⁱ

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingⁱ

Molecule processing

Proteomic databases

PTM databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactionⁱ

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structureⁱ

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction%5Fsection">Interaction</a>' section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="https://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated at every <a href="http://www.uniprot.org/help/synchronization">UniProt release</a>.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsⁱ

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structureⁱ

Secondary structure

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsⁱ

Region

Coiled coil

Compositional bias

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesⁱ

Keywords - Domainⁱ

Phylogenomic databases

Family and domain databases

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequenceⁱ

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusⁱ: Complete.

        10         20         30         40         50
MSQTINSELN ENATSQCKED LKVSLSESDL RDSQGQLGIE NPPKCNNSGN 
        60         70         80         90        100
HSDNLGFIEQ SETVHPENEK ALTPDLRDTK IHTSLPITTP FSKKRAREAK 
       110        120        130        140        150
NILLKPFKSP LRQTASPQVA DTNLKPSLAV TNLNSDETNT SSEPVTSPLR 
       160        170        180        190        200
TTPNSIKRQK RLFKSPISNC LNPKSDPEIT QLLSRRLKLE KEVRNLQEQL 
       210        220        230        240        250
ITAETARKVE AKNEDKDLQT LIQKWKNAAQ QAAEVLFKPM AERIRLAGGV 
       260        270        280        290 
TQSFRIEEGE NKGQIQEVRT EFTMSMFLNQ FGVPVHLMSF DEENGDWKS  

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesⁱ

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationⁱ

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousⁱ

Keywords - Technical termⁱ

Documents

1. Schizosaccharomyces pombe
   Schizosaccharomyces pombe: entries and gene names
2. PDB cross-references
   Index of Protein Data Bank (PDB) cross-references
3. SIMILARITY comments
   Index of protein domains and families