UniProtKB - Q9USQ7 (DPH6_SCHPO)
Protein
Diphthine--ammonia ligase
Gene
mug71
Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Functioni
Amidase that catalyzes the last step of diphthamide biosynthesis using ammonium and ATP. Diphthamide biosynthesis consists in the conversion of an L-histidine residue in the translation elongation factor eEF-2 (eft201 or eft202) to diphthamide (By similarity). Has a role in meiosis.By similarity1 Publication
Catalytic activityi
- ATP + diphthine-[translation elongation factor 2] + NH4+ = AMP + diphosphate + diphthamide-[translation elongation factor 2] + H+EC:6.3.1.14
: peptidyl-diphthamide biosynthesis Pathwayi
This protein is involved in the pathway peptidyl-diphthamide biosynthesis, which is part of Protein modification.View all proteins of this organism that are known to be involved in the pathway peptidyl-diphthamide biosynthesis and in Protein modification.
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- diphthine-ammonia ligase activity Source: PomBase
GO - Biological processi
- meiotic cell cycle Source: UniProtKB-KW
- peptidyl-diphthamide biosynthetic process from peptidyl-histidine Source: PomBase
Keywordsi
Molecular function | Ligase |
Biological process | Meiosis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-SPO-5358493, Synthesis of diphthamide-EEF2 |
UniPathwayi | UPA00559 |
Names & Taxonomyi
Protein namesi | Recommended name: Diphthine--ammonia ligase (EC:6.3.1.14)Alternative name(s): Diphthamide synthase Diphthamide synthetase |
Gene namesi | Name:mug71 ORF Names:SPBC577.12 |
Organismi | Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) |
Taxonomic identifieri | 284812 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › |
Proteomesi |
|
Organism-specific databases
PomBasei | SPBC577.12 |
VEuPathDBi | FungiDB:SPBC577.12 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000278564 | 1 – 606 | Diphthine--ammonia ligaseAdd BLAST | 606 |
Proteomic databases
MaxQBi | Q9USQ7 |
PaxDbi | Q9USQ7 |
PRIDEi | Q9USQ7 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 277240, 14 interactors |
STRINGi | 4896.SPBC577.12.1 |
Family & Domainsi
Sequence similaritiesi
In the N-terminal section; belongs to the Diphthine--ammonia ligase family.Curated
In the C-terminal section; belongs to the RutC family.Curated
Phylogenomic databases
eggNOGi | KOG2316, Eukaryota KOG2317, Eukaryota |
HOGENOMi | CLU_010289_2_1_1 |
InParanoidi | Q9USQ7 |
OMAi | SNSFGRH |
PhylomeDBi | Q9USQ7 |
Family and domain databases
CDDi | cd01994, Alpha_ANH_like_IV, 1 hit |
Gene3Di | 3.30.1330.40, 2 hits 3.40.50.620, 1 hit |
InterProi | View protein in InterPro IPR002761, Diphthami_syn_dom IPR030662, DPH6/MJ0570 IPR014729, Rossmann-like_a/b/a_fold IPR035959, RutC-like_sf IPR006175, YjgF/YER057c/UK114 |
PANTHERi | PTHR12196, PTHR12196, 1 hit |
Pfami | View protein in Pfam PF01902, Diphthami_syn_2, 1 hit PF01042, Ribonuc_L-PSP, 2 hits |
SUPFAMi | SSF55298, SSF55298, 2 hits |
TIGRFAMsi | TIGR00290, MJ0570_dom, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9USQ7-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKVLGLISGG KDSCFNLMHC VSLGHEVVAL ANLHPEDGKD EIDSFMYQSV
60 70 80 90 100
GHDVIPLYAE CFDLPLYREK IGGQSINQNL DYQFTEKDET EDLYRLIKRV
110 120 130 140 150
LTNHPDLEAV STGAILSTYQ RTRVENVCKR LGLKSLSFLW QKDQEKLLND
160 170 180 190 200
MVVSGLNAIL IKVAAIGLTR KDLGKSLAEM QDKLLTLNKK FELHPCGEGG
210 220 230 240 250
EYETLVLDCP LFKKRIVLTD KEVVEHSSGE VCYLKVKACV KDKPEWQPIS
260 270 280 290 300
LKSELVPNEE LLGEEYSHIY HTISKKYELI DDQEETPTSL IPIPLRESAF
310 320 330 340 350
QQKKGSFLVL GNVVATKGSY NTFQGEAESA INNLNELLGT YGYSNKNVYF
360 370 380 390 400
VTVILSSMSK FAEFNSVYNK YFDFTNPPSR SCVAAPLASE YRIVMSCIVG
410 420 430 440 450
DVTEKRALHV QGQSYWAPAN IGPYSQSICA NGVVFISGQI GLIPSVMELK
460 470 480 490 500
LHDKIFEMVL ALQHANRVAK AMRVGSLIAC LAYVCDSRDA DCVVKIWSEY
510 520 530 540 550
TKNTGESSPV LVALVDALPR NASVEWQLLY NDSSCDVPLL SSLVTNQTLF
560 570 580 590 600
GSDTAWDVAL LNQNGLRMES SFIHHEHPSA YAIVLNNAFP NSQLLHVRYT
ARDQNV
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CU329671 Genomic DNA Translation: CAB54820.1 |
PIRi | T40556 |
RefSeqi | NP_595310.1, NM_001021217.2 |
Genome annotation databases
EnsemblFungii | SPBC577.12.1; SPBC577.12.1:pep; SPBC577.12 |
GeneIDi | 2540717 |
KEGGi | spo:SPBC577.12 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CU329671 Genomic DNA Translation: CAB54820.1 |
PIRi | T40556 |
RefSeqi | NP_595310.1, NM_001021217.2 |
3D structure databases
SMRi | Q9USQ7 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 277240, 14 interactors |
STRINGi | 4896.SPBC577.12.1 |
Proteomic databases
MaxQBi | Q9USQ7 |
PaxDbi | Q9USQ7 |
PRIDEi | Q9USQ7 |
Genome annotation databases
EnsemblFungii | SPBC577.12.1; SPBC577.12.1:pep; SPBC577.12 |
GeneIDi | 2540717 |
KEGGi | spo:SPBC577.12 |
Organism-specific databases
PomBasei | SPBC577.12 |
VEuPathDBi | FungiDB:SPBC577.12 |
Phylogenomic databases
eggNOGi | KOG2316, Eukaryota KOG2317, Eukaryota |
HOGENOMi | CLU_010289_2_1_1 |
InParanoidi | Q9USQ7 |
OMAi | SNSFGRH |
PhylomeDBi | Q9USQ7 |
Enzyme and pathway databases
UniPathwayi | UPA00559 |
Reactomei | R-SPO-5358493, Synthesis of diphthamide-EEF2 |
Miscellaneous databases
PROi | PR:Q9USQ7 |
Family and domain databases
CDDi | cd01994, Alpha_ANH_like_IV, 1 hit |
Gene3Di | 3.30.1330.40, 2 hits 3.40.50.620, 1 hit |
InterProi | View protein in InterPro IPR002761, Diphthami_syn_dom IPR030662, DPH6/MJ0570 IPR014729, Rossmann-like_a/b/a_fold IPR035959, RutC-like_sf IPR006175, YjgF/YER057c/UK114 |
PANTHERi | PTHR12196, PTHR12196, 1 hit |
Pfami | View protein in Pfam PF01902, Diphthami_syn_2, 1 hit PF01042, Ribonuc_L-PSP, 2 hits |
SUPFAMi | SSF55298, SSF55298, 2 hits |
TIGRFAMsi | TIGR00290, MJ0570_dom, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DPH6_SCHPO | |
Accessioni | Q9USQ7Primary (citable) accession number: Q9USQ7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 20, 2007 |
Last sequence update: | May 1, 2000 | |
Last modified: | February 10, 2021 | |
This is version 110 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Schizosaccharomyces pombe
Schizosaccharomyces pombe: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families