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Protein

Sialidase-3

Gene

NEU3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a role in modulating the ganglioside content of the lipid bilayer at the level of membrane-bound sialyl glycoconjugates.2 Publications

Catalytic activityi

Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.2 Publications

pH dependencei

Optimum pH is 3.8.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei25SubstrateBy similarity1
Binding sitei45SubstrateCurated1
Active sitei50Proton acceptor1 Publication1
Binding sitei179SubstrateCurated1
Binding sitei181SubstrateCurated1
Binding sitei225SubstrateCurated1
Binding sitei245SubstrateCurated1
Binding sitei340SubstrateCurated1
Active sitei370Nucleophile1 Publication1
Active sitei387Sequence analysis1

GO - Molecular functioni

GO - Biological processi

  • carbohydrate metabolic process Source: MGI
  • ganglioside catabolic process Source: UniProtKB
  • glycosphingolipid metabolic process Source: Reactome
  • oligosaccharide catabolic process Source: UniProtKB

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism, Lipid degradation, Lipid metabolism

Enzyme and pathway databases

BRENDAi3.2.1.18 2681
ReactomeiR-HSA-1660662 Glycosphingolipid metabolism
R-HSA-4085001 Sialic acid metabolism
SABIO-RKiQ9UQ49

Protein family/group databases

CAZyiGH33 Glycoside Hydrolase Family 33

Chemistry databases

SwissLipidsiSLP:000001372 [Q9UQ49-1]

Names & Taxonomyi

Protein namesi
Recommended name:
Sialidase-3 (EC:3.2.1.18)
Alternative name(s):
Ganglioside sialidase
Membrane sialidase
N-acetyl-alpha-neuraminidase 3
Gene namesi
Name:NEU3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

EuPathDBiHostDB:ENSG00000162139.9
HGNCiHGNC:7760 NEU3
MIMi604617 gene
neXtProtiNX_Q9UQ49

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi45R → V: Loss of enzyme activity. 1 Publication1
Mutagenesisi50D → S: Nearly abolishes enzyme activity. 1 Publication1
Mutagenesisi179Y → F: Loss of enzyme activity. 1 Publication1
Mutagenesisi181Y → F: Nearly abolishes enzyme activity. 1 Publication1
Mutagenesisi225E → S: Loss of enzyme activity. 1 Publication1
Mutagenesisi245R → A: Loss of enzyme activity. 1 Publication1
Mutagenesisi340R → A: Loss of enzyme activity. 1 Publication1
Mutagenesisi370Y → F: Loss of enzyme activity. 1 Publication1

Organism-specific databases

DisGeNETi10825
OpenTargetsiENSG00000162139
PharmGKBiPA31562

Chemistry databases

ChEMBLiCHEMBL3046

Polymorphism and mutation databases

DMDMi17369720

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002089031 – 428Sialidase-3Add BLAST428

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei313PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9UQ49
PaxDbiQ9UQ49
PeptideAtlasiQ9UQ49
PRIDEiQ9UQ49
ProteomicsDBi85507

PTM databases

iPTMnetiQ9UQ49
PhosphoSitePlusiQ9UQ49
SwissPalmiQ9UQ49

Expressioni

Tissue specificityi

Highly expressed in skeletal muscle, testis, adrenal gland and thymus, followed by pancreas, liver, heart and thymus. Weakly expressed in kidney, placenta, brain and lung.1 Publication

Gene expression databases

BgeeiENSG00000162139
CleanExiHS_NEU3
ExpressionAtlasiQ9UQ49 baseline and differential
GenevisibleiQ9UQ49 HS

Organism-specific databases

HPAiHPA038729
HPA038730
HPA070381

Interactioni

Protein-protein interaction databases

BioGridi116038, 7 interactors
STRINGi9606.ENSP00000294064

Chemistry databases

BindingDBiQ9UQ49

Structurei

3D structure databases

ProteinModelPortaliQ9UQ49
SMRiQ9UQ49
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati129 – 140BNR 1Add BLAST12
Repeati203 – 214BNR 2Add BLAST12
Repeati254 – 265BNR 3Add BLAST12

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi24 – 27FRIP motif4

Sequence similaritiesi

Belongs to the glycosyl hydrolase 33 family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IFVF Eukaryota
ENOG410Y74Z LUCA
GeneTreeiENSGT00390000011171
HOGENOMiHOG000233778
HOVERGENiHBG052608
InParanoidiQ9UQ49
KOiK12357
OMAiHSLMIYS
OrthoDBiEOG091G08BI
PhylomeDBiQ9UQ49

Family and domain databases

InterProiView protein in InterPro
IPR011040 Sialidase
IPR026944 Sialidase-3
IPR026856 Sialidase_fam
IPR036278 Sialidase_sf
PANTHERiPTHR10628 PTHR10628, 1 hit
PTHR10628:SF23 PTHR10628:SF23, 1 hit
PfamiView protein in Pfam
PF13088 BNR_2, 1 hit
SUPFAMiSSF50939 SSF50939, 2 hits

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9UQ49-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEEVTTCSFN SPLFRQEDDR GITYRIPALL YIPPTHTFLA FAEKRSTRRD
60 70 80 90 100
EDALHLVLRR GLRIGQLVQW GPLKPLMEAT LPGHRTMNPC PVWEQKSGCV
110 120 130 140 150
FLFFICVRGH VTERQQIVSG RNAARLCFIY SQDAGCSWSE VRDLTEEVIG
160 170 180 190 200
SELKHWATFA VGPGHGIQLQ SGRLVIPAYT YYIPSWFFCF QLPCKTRPHS
210 220 230 240 250
LMIYSDDLGV TWHHGRLIRP MVTVECEVAE VTGRAGHPVL YCSARTPNRC
260 270 280 290 300
RAEALSTDHG EGFQRLALSR QLCEPPHGCQ GSVVSFRPLE IPHRCQDSSS
310 320 330 340 350
KDAPTIQQSS PGSSLRLEEE AGTPSESWLL YSHPTSRKQR VDLGIYLNQT
360 370 380 390 400
PLEAACWSRP WILHCGPCGY SDLAALEEEG LFGCLFECGT KQECEQIAFR
410 420
LFTHREILSH LQGDCTSPGR NPSQFKSN
Length:428
Mass (Da):48,252
Last modified:May 1, 2000 - v1
Checksum:i35D1DD9359A78C98
GO
Isoform 2 (identifier: Q9UQ49-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MRPADLPPRPMEESPASSSAPTETEEPGSSAEVM

Show »
Length:461
Mass (Da):51,675
Checksum:i37D6EBA8FD7458AD
GO

Sequence cautioni

The sequence CAB96131 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05583915R → Q. Corresponds to variant dbSNP:rs7115499Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0541451M → MRPADLPPRPMEESPASSSA PTETEEPGSSAEVM in isoform 2. 2 Publications1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB008185 mRNA Translation: BAA82611.1
Y18563 mRNA Translation: CAB96131.1 Different initiation.
AK022450 mRNA Translation: BAG51074.1
AK290442 mRNA Translation: BAF83131.1
AP001992 Genomic DNA No translation available.
CH471076 Genomic DNA Translation: EAW74953.1
BC136397 mRNA Translation: AAI36398.1
BC144059 mRNA Translation: AAI44060.1
CCDSiCCDS44682.1 [Q9UQ49-2]
RefSeqiNP_006647.3, NM_006656.5 [Q9UQ49-2]
UniGeneiHs.191074

Genome annotation databases

EnsembliENST00000294064; ENSP00000294064; ENSG00000162139 [Q9UQ49-2]
ENST00000531509; ENSP00000432097; ENSG00000162139 [Q9UQ49-2]
GeneIDi10825
KEGGihsa:10825
UCSCiuc001ovw.4 human [Q9UQ49-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiNEUR3_HUMAN
AccessioniPrimary (citable) accession number: Q9UQ49
Secondary accession number(s): A8K327, Q9NQE1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 16, 2001
Last sequence update: May 1, 2000
Last modified: June 20, 2018
This is version 142 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. SIMILARITY comments
    Index of protein domains and families

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