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Protein

Trafficking kinesin-binding protein 1

Gene

TRAK1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the regulation of endosome-to-lysosome trafficking, including endocytic trafficking of EGF-EGFR complexes and GABA-A receptors (PubMed:18675823). Involved in mitochondrial motility. When O-glycosylated, abolishes mitochondrial motility. Crucial for recruiting OGT to the mitochondrial surface of neuronal processes (PubMed:24995978). TRAK1 and RHOT form an essential protein complex that links KIF5 to mitochondria for light chain-independent, anterograde transport of mitochondria (By similarity).By similarity2 Publications

Miscellaneous

Over-expressed in all investigated carcinomas, especially in gastric adenocarcinoma and signet-ring carcinoma and may serve as a marker of gastric cancer.

GO - Molecular functioni

  • GABA receptor binding Source: GO_Central
  • myosin binding Source: GO_Central
  • signaling receptor binding Source: GO_Central
  • TPR domain binding Source: Ensembl

GO - Biological processi

Enzyme and pathway databases

ReactomeiR-HSA-6802952 Signaling by BRAF and RAF fusions

Names & Taxonomyi

Protein namesi
Recommended name:
Trafficking kinesin-binding protein 1
Alternative name(s):
106 kDa O-GlcNAc transferase-interacting protein
Protein Milton1 Publication
Gene namesi
Name:TRAK1
Synonyms:KIAA1042, OIP106
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

EuPathDBiHostDB:ENSG00000182606.14
HGNCiHGNC:29947 TRAK1
MIMi608112 gene
neXtProtiNX_Q9UPV9

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endosome, Membrane, Mitochondrion, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi447S → A: Reduced O-glycosylation of this protein. 1 Publication1
Mutagenesisi658 – 672Missing : Loss of interaction with OGT, but interacts with KIF5B and RHOT1/2 and is able to localize to mitochondria. Increased O-glycosylation of this protein by OGT. 1 PublicationAdd BLAST15
Mutagenesisi829S → A: Reduced O-glycosylation of this protein; when associated with A-830. 1 Publication1
Mutagenesisi830S → A: Reduced O-glycosylation of this protein; when associated with A-829. 1 Publication1
Mutagenesisi938S → A: Reduced O-glycosylation of this protein. 1 Publication1

Organism-specific databases

DisGeNETi22906
MalaCardsiTRAK1
OpenTargetsiENSG00000182606
Orphaneti442835 Undetermined early-onset epileptic encephalopathy
PharmGKBiPA128394593

Polymorphism and mutation databases

BioMutaiTRAK1
DMDMi13124654

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000580371 – 953Trafficking kinesin-binding protein 1Add BLAST953

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi447O-linked (GlcNAc) serine1 Publication1
Modified residuei537PhosphoserineBy similarity1
Glycosylationi680O-linked (GlcNAc) serine1 Publication1
Modified residuei719PhosphoserineBy similarity1
Glycosylationi719O-linked (GlcNAc) serine1 Publication1
Modified residuei919PhosphoserineCombined sources1
Glycosylationi935O-linked (GalNAc) threonine1 Publication1

Post-translational modificationi

O-glycosylated (PubMed:24995978, PubMed:12435728). Glycosylated by OGT; glycosylation in response to increased extracellular glucose levels is required for and leads to regulation of mitochondrial motility by OGT (PubMed:24995978).2 Publications

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

EPDiQ9UPV9
PaxDbiQ9UPV9
PeptideAtlasiQ9UPV9
PRIDEiQ9UPV9
ProteomicsDBi85456
85457 [Q9UPV9-2]

PTM databases

iPTMnetiQ9UPV9
PhosphoSitePlusiQ9UPV9

Expressioni

Tissue specificityi

High expression in spinal cord and moderate expression in all other tissues and specific brain regions examined. Expressed in all cell lines examined.1 Publication

Gene expression databases

BgeeiENSG00000182606 Expressed in 236 organ(s), highest expression level in secondary oocyte
CleanExiHS_TRAK1
ExpressionAtlasiQ9UPV9 baseline and differential
GenevisibleiQ9UPV9 HS

Organism-specific databases

HPAiHPA005853
HPA011367

Interactioni

Subunit structurei

Interacts with RHOT1 and RHOT2 (PubMed:16630562). Found in a complex with KIF5B, OGT, RHOT1 and RHOT2 (PubMed:24995978). Interacts with HGS (PubMed:18675823). Interacts with GABRA1 (By similarity). Interacts with KIF5C (PubMed:15644324). Interacts with OGT; stable interaction is not required for glycosylation of this protein by OGT. Isoform 1 interacts with OGT (PubMed:24995978, PubMed:12435728).By similarity5 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi116570, 10 interactors
CORUMiQ9UPV9
IntActiQ9UPV9, 16 interactors
MINTiQ9UPV9
STRINGi9606.ENSP00000328998

Structurei

3D structure databases

ProteinModelPortaliQ9UPV9
SMRiQ9UPV9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini47 – 354HAP1 N-terminalAdd BLAST308

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni359 – 509Interaction with HGS1 PublicationAdd BLAST151
Regioni658 – 672Interaction with OGT1 PublicationAdd BLAST15

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili104 – 356Sequence analysisAdd BLAST253
Coiled coili492 – 532Sequence analysisAdd BLAST41

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi200 – 203Poly-Ser4

Domaini

The C-terminal region is required for the early endosomal and mitochondrial localization.

Sequence similaritiesi

Belongs to the milton family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG4360 Eukaryota
ENOG410XSWD LUCA
GeneTreeiENSGT00390000003132
HOGENOMiHOG000143414
HOVERGENiHBG069248
InParanoidiQ9UPV9
KOiK15369
OMAiQRKVNTL
OrthoDBiEOG091G0LLD
PhylomeDBiQ9UPV9
TreeFamiTF323495

Family and domain databases

InterProiView protein in InterPro
IPR006933 HAP1_N
IPR022154 Traffickng_kinesin-bd_prot_dom
PfamiView protein in Pfam
PF04849 HAP1_N, 1 hit
PF12448 Milton, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 6 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q9UPV9-1) [UniParc]FASTAAdd to basket
Also known as: Milton11 Publication

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MALVFQFGQP VRAQPLPGLC HGKLIRTNAC DVCNSTDLPE VEIISLLEEQ
60 70 80 90 100
LPHYKLRADT IYGYDHDDWL HTPLISPDAN IDLTTEQIEE TLKYFLLCAE
110 120 130 140 150
RVGQMTKTYN DIDAVTRLLE EKERDLELAA RIGQSLLKKN KTLTERNELL
160 170 180 190 200
EEQVEHIREE VSQLRHELSM KDELLQFYTS AAEESEPESV CSTPLKRNES
210 220 230 240 250
SSSVQNYFHL DSLQKKLKDL EEENVVLRSE ASQLKTETIT YEEKEQQLVN
260 270 280 290 300
DCVKELRDAN VQIASISEEL AKKTEDAARQ QEEITHLLSQ IVDLQKKAKA
310 320 330 340 350
CAVENEELVQ HLGAAKDAQR QLTAELRELE DKYAECMEML HEAQEELKNL
360 370 380 390 400
RNKTMPNTTS RRYHSLGLFP MDSLAAEIEG TMRKELQLEE AESPDITHQK
410 420 430 440 450
RVFETVRNIN QVVKQRSLTP SPMNIPGSNQ SSAMNSLLSS CVSTPRSSFY
460 470 480 490 500
GSDIGNVVLD NKTNSIILET EAADLGNDER SKKPGTPGTP GSHDLETALR
510 520 530 540 550
RLSLRRENYL SERRFFEEEQ ERKLQELAEK GELRSGSLTP TESIMSLGTH
560 570 580 590 600
SRFSEFTGFS GMSFSSRSYL PEKLQIVKPL EGSATLHHWQ QLAQPHLGGI
610 620 630 640 650
LDPRPGVVTK GFRTLDVDLD EVYCLNDFEE DDTGDHISLP RLATSTPVQH
660 670 680 690 700
PETSAHHPGK CMSQTNSTFT FTTCRILHPS DELTRVTPSL NSAPTPACGS
710 720 730 740 750
TSHLKSTPVA TPCTPRRLSL AESFTNTRES TTTMSTSLGL VWLLKERGIS
760 770 780 790 800
AAVYDPQSWD RAGRGSLLHS YTPKMAVIPS TPPNSPMQTP TSSPPSFEFK
810 820 830 840 850
CTSPPYDNFL ASKPASSILR EVREKNVRSS ESQTDVSVSN LNLVDKVRRF
860 870 880 890 900
GVAKVVNSGR AHVPTLTEEQ GPLLCGPPGP APALVPRGLV PEGLPLRCPT
910 920 930 940 950
VTSAIGGLQL NSGIRRNRSF PTMVGSSMQM KAPVTLTSGI LMGAKLSKQT

SLR
Length:953
Mass (Da):106,040
Last modified:May 1, 2000 - v1
Checksum:iE834BE07CF41D9A4
GO
Isoform 2 (identifier: Q9UPV9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-96: MALVFQFGQP...QIEETLKYFL → MSLRDKGGEE...HDTQDLLEEV
     655-744: AHHPGKCMSQ...STSLGLVWLL → GERSQARVTV...LSVASARLCG
     745-953: Missing.

Show »
Length:686
Mass (Da):77,256
Checksum:iE14EC7B6B460E042
GO
Isoform 3 (identifier: Q9UPV9-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-97: MALVFQFGQP...IEETLKYFLL → MQKFIEADYYELDWYYEECSDVL
     583-630: SATLHHWQQL...EVYCLNDFEE → DHAGPRPLSV...SHPRCWFEFL
     631-953: Missing.

Note: No experimental confirmation available.
Show »
Length:556
Mass (Da):63,642
Checksum:i9837FDB279C91758
GO

Computationally mapped potential isoform sequencesi

There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
C9JC32C9JC32_HUMAN
Trafficking kinesin-binding protein...
TRAK1 hCG_2000014
895Annotation score:
A0A087X0N0A0A087X0N0_HUMAN
Trafficking kinesin-binding protein...
TRAK1
670Annotation score:
H7C3T3H7C3T3_HUMAN
Trafficking kinesin-binding protein...
TRAK1
300Annotation score:
A0A0D9SFL5A0A0D9SFL5_HUMAN
Trafficking kinesin-binding protein...
TRAK1
849Annotation score:
A0A0D9SGH2A0A0D9SGH2_HUMAN
Trafficking kinesin-binding protein...
TRAK1
526Annotation score:
F8WDH2F8WDH2_HUMAN
Trafficking kinesin-binding protein...
TRAK1
44Annotation score:

Sequence cautioni

The sequence BAA82994 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti503S → P in CAH56169 (PubMed:17974005).Curated1
Sequence conflicti638S → F in CAH56169 (PubMed:17974005).Curated1
Isoform 2 (identifier: Q9UPV9-2)
Sequence conflicti631E → EE in CAH56169 (PubMed:17974005).Curated1
Sequence conflicti631E → EEE in AAH15922 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0455581 – 97MALVF…KYFLL → MQKFIEADYYELDWYYEECS DVL in isoform 3. 1 PublicationAdd BLAST97
Alternative sequenceiVSP_0108391 – 96MALVF…LKYFL → MSLRDKGGEEECFEYDCQDE ERKPTHRQHDTQDLLEEV in isoform 2. 2 PublicationsAdd BLAST96
Alternative sequenceiVSP_045559583 – 630SATLH…NDFEE → DHAGPRPLSVLLGDSLWSLI HLRKAGHLCHAYSFFFRDSH PRCWFEFL in isoform 3. 1 PublicationAdd BLAST48
Alternative sequenceiVSP_045560631 – 953Missing in isoform 3. 1 PublicationAdd BLAST323
Alternative sequenceiVSP_010840655 – 744AHHPG…LVWLL → GERSQARVTVSGSRSYPSRP QASPEEMQEPPAATEEEEEE EEEEGSGEGTTISPVNLAPF PEAEFWAILTSVPGTIRSGS LSVASARLCG in isoform 2. 2 PublicationsAdd BLAST90
Alternative sequenceiVSP_010841745 – 953Missing in isoform 2. 2 PublicationsAdd BLAST209

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB028965 mRNA Translation: BAA82994.2 Different initiation.
BX647199 mRNA Translation: CAH56169.1
AL713787 mRNA Translation: CAH56394.1
AC018358 Genomic DNA No translation available.
AC093414 Genomic DNA No translation available.
AC137935 Genomic DNA No translation available.
BC015922 mRNA Translation: AAH15922.1
CCDSiCCDS2695.1 [Q9UPV9-2]
CCDS43072.1 [Q9UPV9-1]
CCDS58826.1 [Q9UPV9-3]
RefSeqiNP_001036111.1, NM_001042646.2 [Q9UPV9-1]
NP_001252537.1, NM_001265608.1
NP_001252539.1, NM_001265610.1 [Q9UPV9-3]
NP_055780.2, NM_014965.4 [Q9UPV9-2]
UniGeneiHs.535711

Genome annotation databases

EnsembliENST00000327628; ENSP00000328998; ENSG00000182606 [Q9UPV9-1]
ENST00000341421; ENSP00000340702; ENSG00000182606 [Q9UPV9-2]
ENST00000449246; ENSP00000410717; ENSG00000182606 [Q9UPV9-3]
GeneIDi22906
KEGGihsa:22906
UCSCiuc003cky.5 human [Q9UPV9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB028965 mRNA Translation: BAA82994.2 Different initiation.
BX647199 mRNA Translation: CAH56169.1
AL713787 mRNA Translation: CAH56394.1
AC018358 Genomic DNA No translation available.
AC093414 Genomic DNA No translation available.
AC137935 Genomic DNA No translation available.
BC015922 mRNA Translation: AAH15922.1
CCDSiCCDS2695.1 [Q9UPV9-2]
CCDS43072.1 [Q9UPV9-1]
CCDS58826.1 [Q9UPV9-3]
RefSeqiNP_001036111.1, NM_001042646.2 [Q9UPV9-1]
NP_001252537.1, NM_001265608.1
NP_001252539.1, NM_001265610.1 [Q9UPV9-3]
NP_055780.2, NM_014965.4 [Q9UPV9-2]
UniGeneiHs.535711

3D structure databases

ProteinModelPortaliQ9UPV9
SMRiQ9UPV9
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116570, 10 interactors
CORUMiQ9UPV9
IntActiQ9UPV9, 16 interactors
MINTiQ9UPV9
STRINGi9606.ENSP00000328998

PTM databases

iPTMnetiQ9UPV9
PhosphoSitePlusiQ9UPV9

Polymorphism and mutation databases

BioMutaiTRAK1
DMDMi13124654

Proteomic databases

EPDiQ9UPV9
PaxDbiQ9UPV9
PeptideAtlasiQ9UPV9
PRIDEiQ9UPV9
ProteomicsDBi85456
85457 [Q9UPV9-2]

Protocols and materials databases

DNASUi22906
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000327628; ENSP00000328998; ENSG00000182606 [Q9UPV9-1]
ENST00000341421; ENSP00000340702; ENSG00000182606 [Q9UPV9-2]
ENST00000449246; ENSP00000410717; ENSG00000182606 [Q9UPV9-3]
GeneIDi22906
KEGGihsa:22906
UCSCiuc003cky.5 human [Q9UPV9-1]

Organism-specific databases

CTDi22906
DisGeNETi22906
EuPathDBiHostDB:ENSG00000182606.14
GeneCardsiTRAK1
HGNCiHGNC:29947 TRAK1
HPAiHPA005853
HPA011367
MalaCardsiTRAK1
MIMi608112 gene
neXtProtiNX_Q9UPV9
OpenTargetsiENSG00000182606
Orphaneti442835 Undetermined early-onset epileptic encephalopathy
PharmGKBiPA128394593
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4360 Eukaryota
ENOG410XSWD LUCA
GeneTreeiENSGT00390000003132
HOGENOMiHOG000143414
HOVERGENiHBG069248
InParanoidiQ9UPV9
KOiK15369
OMAiQRKVNTL
OrthoDBiEOG091G0LLD
PhylomeDBiQ9UPV9
TreeFamiTF323495

Enzyme and pathway databases

ReactomeiR-HSA-6802952 Signaling by BRAF and RAF fusions

Miscellaneous databases

ChiTaRSiTRAK1 human
GeneWikiiTRAK1
GenomeRNAii22906
PROiPR:Q9UPV9
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000182606 Expressed in 236 organ(s), highest expression level in secondary oocyte
CleanExiHS_TRAK1
ExpressionAtlasiQ9UPV9 baseline and differential
GenevisibleiQ9UPV9 HS

Family and domain databases

InterProiView protein in InterPro
IPR006933 HAP1_N
IPR022154 Traffickng_kinesin-bd_prot_dom
PfamiView protein in Pfam
PF04849 HAP1_N, 1 hit
PF12448 Milton, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTRAK1_HUMAN
AccessioniPrimary (citable) accession number: Q9UPV9
Secondary accession number(s): E9PDS2
, J3KNT7, Q63HR0, Q659B5, Q96B69
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: May 1, 2000
Last modified: November 7, 2018
This is version 146 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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